The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Ana Rita Coimbra Motta-castro - One of the best experts on this subject based on the ideXlab platform.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Flavio Augusto Pádua Milagres, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Erotildes Leal, Ana Rita Coimbra Motta-castroAbstract:Background<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies.MethodsOral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization.ResultsIn optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded.ConclusionsIt was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.
Bo L. Lönnerdal - One of the best experts on this subject based on the ideXlab platform.
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Maternal zinc supplementation improves <B>HepatitisB> B antiBody responses in infants But decreases plasma zinc level
European Journal of Nutrition, 2016Co-Authors: Shaikh Meshbahuddin Ahmad, Mohammad Bakhtiar Hossain, Md. Monirujjaman, Sharmin Islam, Md. Nazmul Huda, Yearul Kabir, Rubhana Raqib, Bo L. LönnerdalAbstract:Purpose The World Health Report identifies zinc deficiency as one of the major causes of disease in developing countries, and infants are at particular risk. We aimed to investigate the effect of maternal zinc supplementation on the infant’s immune function in a population at risk of deficiency. Methods In a randomized, douBle-Blind placeBo-controlled trial, mothers were supplemented either with 20 mg/day of elemental zinc ( n = 20) or placeBo ( n = 19) at the Beginning of second trimester, which continued until 6 months postpartum. Indicators of the infants’ immune function measured included interleukin (IL)-7, thymic size and response to <B>HepatitisB> B vaccination. Results Infants Born from mothers receiving zinc supplements during pregnancy and postpartum had significantly lower plasma zinc ( p
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Maternal zinc supplementation improves <B>HepatitisB> B antiBody responses in infants But decreases plasma zinc level
European Journal of Nutrition, 2015Co-Authors: Meshbahuddin Ahmad, Mohammad Bakhtiar Hossain, Sharmin Islam, Yearul Kabir, Rubhana Raqib, Monirujjaman, Nazmul Huda, Bo L. LönnerdalAbstract:Purpose The World Health Report identifies zinc deficiency as one of the major causes of disease in developing countries, and infants are at particular risk. We aimed to investigate the effect of maternal zinc supplementation on the infant’s immune function in a population at risk of deficiency.
Helena Medina Cruz - One of the best experts on this subject based on the ideXlab platform.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Flavio Augusto Pádua Milagres, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Erotildes Leal, Ana Rita Coimbra Motta-castroAbstract:Background<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies.MethodsOral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization.ResultsIn optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded.ConclusionsIt was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Kycia Maria Rodrigues Do Ó, Flavio Augusto De Pádua Milagres, Erotildes Maria LealAbstract:<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies. Oral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization. In optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded. It was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.
Elisangela Ferreira Silva - One of the best experts on this subject based on the ideXlab platform.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Flavio Augusto Pádua Milagres, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Erotildes Leal, Ana Rita Coimbra Motta-castroAbstract:Background<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies.MethodsOral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization.ResultsIn optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded.ConclusionsIt was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Kycia Maria Rodrigues Do Ó, Flavio Augusto De Pádua Milagres, Erotildes Maria LealAbstract:<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies. Oral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization. In optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded. It was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.
Marcelo Santos Cruz - One of the best experts on this subject based on the ideXlab platform.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Flavio Augusto Pádua Milagres, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Erotildes Leal, Ana Rita Coimbra Motta-castroAbstract:Background<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies.MethodsOral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization.ResultsIn optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded.ConclusionsIt was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.
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Utility of oral fluid samples for <B>HepatitisB> B antiBody detection in real life conditions
BMC Infectious Diseases, 2019Co-Authors: Helena Medina Cruz, Vanessa Salete De Paula, Elisangela Ferreira Silva, Marcelo Santos Cruz, Francisco Inácio Bastos, Jurema Corrêa Mota, Priscila Pollo-flores, Kycia Maria Rodrigues Do Ó, Flavio Augusto De Pádua Milagres, Erotildes Maria LealAbstract:<B>HepatitisB> B virus (HBV) testing in oral fluid samples may provide advantages in diagnosis, screening or prevalence studies, especially among individuals with venous access difficulties. This study aims to optimize one commercially availaBle assay for detecting total anti-HBc marker in oral fluid samples and to evaluate its utility under real life conditions in different settings for the purposes of prevalence and diagnostic studies. Oral fluid was collected using a Salivette device and some parameters were initially evaluated: type of elution Buffer and sample volume. Thereafter, the utility of oral fluid samples for detection of anti-HBc was evaluated in real life conditions in which, 1296 individuals gave serum and oral fluid samples. All serum samples were suBmitted to commercial EIAs to detect total anti-HBc, according to the manufacturer’s instructions and oral fluid samples according to previous optimization. In optimization evaluation, PBS/BSA 0.5% and 100 μL of oral fluid (volume was two-fold increased compared to serum in EIA) were chosen as transport Buffer and sample volume. In the field study, anti-HBc was detected in 211 out of 1296 serum samples giving overall oral fluid sensitivity of 52.6% and specificity of 96%. Concordance was higher in amBulatory setting (67.7) compared to general population (31.8). Mean ± standard deviation values of optical density/cutoff (OD/CO) in serum samples were higher in false-negative oral fluid samples than those seen in true positive samples. Sensitivity was higher in those presenting active infection compared to anti-HBc isolate and past infection. Sensitivity also increased in the amBulatory group when HCV individuals were excluded. It was possiBle to optimize a commercial EIA for detecting anti-HBc in oral fluid samples and where the highest concordance was found in amBulatory settings and among individuals with active infection.