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Yunlong Zhao - One of the best experts on this subject based on the ideXlab platform.
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effects of exposure to waterborne polystyrene microspheres on lipid metabolism in the Hepatopancreas of juvenile redclaw crayfish cherax quadricarinatus
Aquatic Toxicology, 2020Co-Authors: Qiang Chen, Yang Jiao, Zhiquan Liu, Mingqi Cai, Wenzong Zhou, Yunlong ZhaoAbstract:Previous research has identified microplastics as new environmental pollutants that are widely distributed in a variety of environments, including aquaculture environments. However, the potential hazard of microplastics to aquaculture animals, especially toward lipid metabolism involved with the survival and growth of aquatic animal, has not yet been investigated. In the present study, redclaw crayfish (Cherax quadricarinatus) were exposed to different concentrations of 200 nm-sized polystyrene microspheres (0, 0.5, and 5 mg/L) for 21 days, to investigate the effects of microplastics on lipid metabolism. After ingestion, the microplastics were distributed in the intestines and Hepatopancreas, and appeared to inhibit the growth of Cherax quadricarinatus. Subsequently, the lipid levels in the Hepatopancreas and hemolymph was detected, and found that after 21 days of exposure, the lipid content and free fatty acids in the Hepatopancreas and hemolymph decreased significantly, and total cholesterol and triglycerides levels increased significantly in the hemolymph. This might have been caused by insufficient intake of exogenous fat. A significant decrease in lipase activity also supported this view. The activity of lipoprotein lipase related to lipolysis in the Hepatopancreas increased significantly, while the activity of fatty acid synthase related to fat synthesis increased, and the activity of acetyl-CoA carboxylase decreased. These results indicated disturbed lipid metabolism in the Hepatopancreas. The significant increase of lipid transport-related low-density lipoprotein indicated that the lipolytic capacity was higher than the lipid synthesis capacity. The expression levels of fatty acid metabolism-related genes FAD6 and FABP decreased significantly, indicating that the fatty acid utilization ability of Hepatopancreas cells was inhibited, which was consistent with the results of enzyme activities. Thus, microplastics represent a potential hazard to redclaw crayfish, at least on lipid metabolism. This study provided basic data on the ecotoxicological effects of microplastics on crustaceans.
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toxic effects of copper on antioxidative and metabolic enzymes of the marine gastropod onchidium struma
Archives of Environmental Contamination and Toxicology, 2009Co-Authors: Xueli Hou, Yongxu Cheng, Qian Mao, Yunlong Zhao, Qun WangAbstract:The goals of this study were to evaluate the acute and sublethal toxicity of copper (Cu2+) on the marine gastropod, Onchidium struma, and to examine the utility of enzymatic parameters as indicators of Cu2+ exposure. In a semistatic renewal test, the 96-hour median lethal concentration of Cu2+ for O. struma, 74.80 mg/L, was higher than that for other intertidal species. The activities of the antioxidative enzymes, Cu/Zn superoxide dismutase (Cu/Zn-SOD) and catalase (CAT), and those of the metabolic enzymes-acid phosphatase (ACP), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) -in both Hepatopancreas and muscle were determined after a 1-week exposure to Cu2+ (range 1.35 to 4.20 mg/L). The activities of both Cu/Zn-SOD and CAT were higher in Hepatopancreas than muscle. In addition, there was a negative correlation between Cu2+ concentration and Cu/Zn-SOD activity in Hepatopancreas, whereas a positive correlation was observed for CAT activity. Concentration-dependent changes in ACP and AKP activity showed a similar trend in Hepatopancreas, increasing then decreasing and, finally, a slight increase. In contrast, ACP activity was positively correlated with Cu2+ across the concentration range tested. In both Hepatopancreas and muscle, both GOT and GPT were activated by lower concentrations of Cu2+ and inhibited at higher concentrations.
Chris G. Carter - One of the best experts on this subject based on the ideXlab platform.
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Relationship between abdominal muscle carbon content (AMN) and Hepatopancreas dry matter content (HPDM), Hepatopancreas total lipid content (HPTL), and haemolymph total protein (TP) for all three species, Sagmariasus verreauxi, Jasus edwardsii, and P
2016Co-Authors: Cedric J. Simon, Thomas Rodemann, Chris G. CarterAbstract:Relationship between abdominal muscle carbon content (AMN) and Hepatopancreas dry matter content (HPDM), Hepatopancreas total lipid content (HPTL), and haemolymph total protein (TP) for all three species, Sagmariasus verreauxi, Jasus edwardsii, and Panilurus ornatus.
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Relationship between abdominal muscle nitrogen content (AMC) and Hepatopancreas dry matter content (HPDM), Hepatopancreas total lipid content (HPTL), and haemolymph total protein (TP) for all three species, Sagmariasus verreauxi, Jasus edwardsii, and
2016Co-Authors: Cedric J. Simon, Thomas Rodemann, Chris G. CarterAbstract:Relationship between abdominal muscle nitrogen content (AMC) and Hepatopancreas dry matter content (HPDM), Hepatopancreas total lipid content (HPTL), and haemolymph total protein (TP) for all three species, Sagmariasus verreauxi, Jasus edwardsii, and Panilurus ornatus.
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Relationship between abdominal muscle dry matter content (AMDM) and Hepatopancreas dry matter content (HPDM), Hepatopancreas total lipid content (HPTL), and haemolymph total protein (TP) for all three species, Sagmariasus verreauxi, Jasus edwardsii,
2016Co-Authors: Cedric J. Simon, Thomas Rodemann, Chris G. CarterAbstract:Relationship between abdominal muscle dry matter content (AMDM) and Hepatopancreas dry matter content (HPDM), Hepatopancreas total lipid content (HPTL), and haemolymph total protein (TP) for all three species, Sagmariasus verreauxi, Jasus edwardsii, and Panilurus ornatus.
Yongxu Cheng - One of the best experts on this subject based on the ideXlab platform.
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immunolocalization and changes of 17beta estradiol during ovarian development of chinese mitten crab eriocheir sinensis
Cell and Tissue Research, 2018Co-Authors: Jie Pan, Meimei Liu, Ting Chen, Yongxu ChengAbstract:17beta-estradiol (E2) is important for crustacean ovarian development. This study aims to investigate the distribution and change pattern of E2 in the ovary, Hepatopancreas, thoracic ganglion and brain ganglion as well as Vg-mRNA expression level during ovarian development of Chinese mitten crab Eriocheir sinensis. Results showed that strongly positive signals of E2 were mainly distributed in follicle cells of ovaries for all developmental stages as well as oocyte cytoplasm of stages III to V ovaries. In Hepatopancreas, the E2-positive signal was mainly detected in the cytoplasm and nucleus of fibrillar cells and the nucleus of resorptive cells, while the maximum fluorescence intensity was observed in stage III Hepatopancreas. On the contrary, the E2 immunoreactivities in nervous tissues were relatively stable during ovarian development. Moreover, the changing pattern of E2 concentration was similar within hemolymph, ovary and Hepatopancreas during the ovarian development. From stages I to III, the E2 content in three tissues increased significantly, then decreased gradually until stage V. As for the Vg-mRNA expression level in Hepatopancreas and ovaries, an increasing trend was found in ovaries but no significant difference was detected during the period of ovarian stages III to V. Hepatopancreatic Vg-mRNA expression level increased significantly during stages I to IV and dramatically decreased at stage V. In conclusion, our study suggests that ovary, Hepatopancreas, hemolymph and nervous tissues are the target organs of E2 in E. sinensis and E2 concentrations in different tissues are closely related to vitellogenesis in ovary and Hepatopancreas during ovarian development.
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insights into hepatopancreatic functions for nutrition metabolism and ovarian development in the crab portunus trituberculatus gene discovery in the comparative transcriptome of different Hepatopancreas stages
PLOS ONE, 2014Co-Authors: Wei Wang, Zhijun Liu, Huajun Zheng, Yongxu ChengAbstract:The crustacean Hepatopancreas has different functions including absorption, storage of nutrients and vitellogenesis during growth, and ovarian development. However, genetic information on the biological functions of the crustacean Hepatopancreas during such processes is limited. The swimming crab, Portunus trituberculatus, is a commercially important species for both aquaculture and fisheries in the Asia-Pacific region. This study compared the transcriptome in the Hepatopancreas of female P. trituberculatus during the growth and ovarian maturation stages by 454 high-throughput pyrosequencing and bioinformatics. The goal was to discover genes in the Hepatopancreas involved in food digestion, nutrition metabolism and ovarian development, and to identify patterns of gene expression during growth and ovarian maturation. Our transcriptome produced 303,450 reads with an average length of 351 bp, and the high quality reads were assembled into 21,635 contigs and 31,844 singlets. Based on BLASTP searches of the deduced protein sequences, there were 7,762 contigs and 4,098 singlets with functional annotation. Further analysis revealed 33,427 unigenes with ORFs, including 17,388 contigs and 16,039 singlets in the Hepatopancreas, while only 7,954 unigenes (5,691 contigs and 2,263 singlets) with the predicted protein sequences were annotated with biological functions. The deduced protein sequences were assigned to 3,734 GO terms, 25 COG categories and 294 specific pathways. Furthermore, there were 14, 534, and 22 identified unigenes involved in food digestion, nutrition metabolism and ovarian development, respectively. 212 differentially expressed genes (DEGs) were found between the growth and endogenous stage of the Hepatopancreas, while there were 382 DEGs between the endogenous and exogenous stage Hepatopancreas. Our results not only enhance the understanding of crustacean hepatopancreatic functions during growth and ovarian development, but also represent a basis for further research on new genes and functional genomics of P. trituberculatus or closely related species.
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toxic effects of copper on antioxidative and metabolic enzymes of the marine gastropod onchidium struma
Archives of Environmental Contamination and Toxicology, 2009Co-Authors: Xueli Hou, Yongxu Cheng, Qian Mao, Yunlong Zhao, Qun WangAbstract:The goals of this study were to evaluate the acute and sublethal toxicity of copper (Cu2+) on the marine gastropod, Onchidium struma, and to examine the utility of enzymatic parameters as indicators of Cu2+ exposure. In a semistatic renewal test, the 96-hour median lethal concentration of Cu2+ for O. struma, 74.80 mg/L, was higher than that for other intertidal species. The activities of the antioxidative enzymes, Cu/Zn superoxide dismutase (Cu/Zn-SOD) and catalase (CAT), and those of the metabolic enzymes-acid phosphatase (ACP), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) -in both Hepatopancreas and muscle were determined after a 1-week exposure to Cu2+ (range 1.35 to 4.20 mg/L). The activities of both Cu/Zn-SOD and CAT were higher in Hepatopancreas than muscle. In addition, there was a negative correlation between Cu2+ concentration and Cu/Zn-SOD activity in Hepatopancreas, whereas a positive correlation was observed for CAT activity. Concentration-dependent changes in ACP and AKP activity showed a similar trend in Hepatopancreas, increasing then decreasing and, finally, a slight increase. In contrast, ACP activity was positively correlated with Cu2+ across the concentration range tested. In both Hepatopancreas and muscle, both GOT and GPT were activated by lower concentrations of Cu2+ and inhibited at higher concentrations.
Chenghui Wang - One of the best experts on this subject based on the ideXlab platform.
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tissue expression profiles unveil the gene interaction of Hepatopancreas eyestalk and ovary in the precocious female chinese mitten crab eriocheir sinensis
BMC Genetics, 2019Co-Authors: Xiaowen Chen, Jun Wang, Xin Hou, Wucheng Yue, Shu Huang, Chenghui WangAbstract:Sexual precocity is a common biological phenomenon in animal species. A large number of precocity individuals were identified in Chinese mitten crab Eriocheir sinensis, which caused huge economic loss annually. However, the underlying genetic basis of precocity in E. sinensis remains unclear to date. In this study, morphological and histological observation and comparative transcriptome analysis were conducted among different stages of precocious one-year-old and normal two-year-old sexually mature E. sinensis. The expression profiles of the ovary, Hepatopancreas, and eyestalk tissues were presented and compared. Genes associated with lipid metabolic process, lipid transport, vitelline membrane formation, vitelline synthesis, and neuropeptide hormone-related genes were upregulated in the ovary, Hepatopancreas, and eyestalk of precocious E. sinensis. Our results indicated that the eyestalk was involved in the neuroendocrine system providing neuropeptide hormones that may induce vitellogenesis in the Hepatopancreas and further stimulate ovary development. The Hepatopancreas is a site for energy storage and vitellogenin synthesis, and it may assist oogenesis through lipid transport in precocious E. sinensis. We provided not only an effective and convenient phenotype measurement method for the identification of potential precocious E. sinensis detection but also valuable genetic resources and novel insights into the molecular mechanism of precocity in E. sinensis. The genetic basis of precocity in E. sinensis is an integrated gene regulatory network of eyestalk, Hepatopancreas, and ovary tissues.
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Tissue expression profiles unveil the gene interaction of Hepatopancreas, eyestalk, and ovary in the precocious female Chinese mitten crab, Eriocheir sinensis
BMC, 2019Co-Authors: Xiaowen Chen, Jun Wang, Xin Hou, Wucheng Yue, Shu Huang, Chenghui WangAbstract:Abstract Background Sexual precocity is a common biological phenomenon in animal species. A large number of precocity individuals were identified in Chinese mitten crab Eriocheir sinensis, which caused huge economic loss annually. However, the underlying genetic basis of precocity in E. sinensis remains unclear to date. Results In this study, morphological and histological observation and comparative transcriptome analysis were conducted among different stages of precocious one-year-old and normal two-year-old sexually mature E. sinensis. The expression profiles of the ovary, Hepatopancreas, and eyestalk tissues were presented and compared. Genes associated with lipid metabolic process, lipid transport, vitelline membrane formation, vitelline synthesis, and neuropeptide hormone-related genes were upregulated in the ovary, Hepatopancreas, and eyestalk of precocious E. sinensis. Our results indicated that the eyestalk was involved in the neuroendocrine system providing neuropeptide hormones that may induce vitellogenesis in the Hepatopancreas and further stimulate ovary development. The Hepatopancreas is a site for energy storage and vitellogenin synthesis, and it may assist oogenesis through lipid transport in precocious E. sinensis. Conclusion We provided not only an effective and convenient phenotype measurement method for the identification of potential precocious E. sinensis detection but also valuable genetic resources and novel insights into the molecular mechanism of precocity in E. sinensis. The genetic basis of precocity in E. sinensis is an integrated gene regulatory network of eyestalk, Hepatopancreas, and ovary tissues
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tissue expression profiles unveils the gene interaction of Hepatopancreas eyestalk and ovary in precocious female chinese mitten crab eriocheir sinensis
bioRxiv, 2018Co-Authors: Jun Wang, Xiaowen Chen, Xin Hou, Wucheng Yue, Shu Huang, Chenghui WangAbstract:Sexual precocity is a serious and common biological phenomenon in animal species. Large amount of precocity individuals was identified in Chinese mitten crab, Eriocheir sinensis , which caused huge economical loss every year. However, the underlying genetic basis of precocity in E. sinensis is still lack. In this study, histology observation, comparative transcriptome was conducted among different stages of precocious one-year old and normal two-year old E. sinensis , tissue-expression profiles of ovary, Hepatopancreas, and eyestalk tissues were presented and compared. Genes associated with lipid metabolic process, lipid transport, vitelline membrane formation, vitelline synthesis and neuropeptide hormone related genes were upregulated in the ovary, Hepatopancreas and eyestalk of precocious E. sinensis . Our results indicated eyestalk involved in neuroendocrine system providing neuropeptide hormone that may induce vitellogenesis in Hepatopancreas and further stimulate ovary development. Hepatopancreas is a site for energy storage, vitellogenin synthesis and may assist to induce oogenesis through lipid transport in precocious E. sinensis . The genetic basis of precocity in E. sinensis is an integrated gene regulatory network of eyestalk, Hepatopancreas, and ovary tissues. Our study provides effective convenient phenotype measurement method for identification of potential precocious E. sinensis detection, and valuable genetic resources and novel insights into the research of molecular mechanism of precocity in E. sinensis .
Josef Velisek - One of the best experts on this subject based on the ideXlab platform.
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effects of terbuthylazine desethyl a terbuthylazine degradation product on red swamp crayfish procambarus clarkii
Science of The Total Environment, 2016Co-Authors: Alzbeta Stara, Eliska Zuskova, Antonin Kouba, Josef VelisekAbstract:Abstract Terbuthylazine is a widely used triazine pesticide. This, together with one of its degradation products, terbuthylazine-desethyl (TD), are frequently found in quantities exceeding the EU limit of 0.1 μg/L in aquatic ecosystems where they might constitute a serious risk to non-target organisms. The sub-chronic effects of TD at 2.9 μg/L (real environmental concentration) and at 580 μg/L were investigated in a non-target aquatic species, the red swamp crayfish ( Procambarus clarkii ). Gill and Hepatopancreas histopathology, alterations in biochemical parameters of haemolymph, oxidative damage to Hepatopancreas, and changes in antioxidant biomarkers in muscle and Hepatopancreas were recorded at both tested concentrations after 14 days exposure. A 14 day recovery period in TD-free water was not sufficient for restoration of normal parameters. Chronic terbuthylazine-desethyl exposure affected biochemical profile, and the antioxidant system, caused oxidative stress and histopathological changes in Hepatopancreas of red swamp crayfish.
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the effect of subchronic metribuzin exposure to signal crayfish pacifastacus leniusculus dana 1852
Neuro endocrinology letters, 2014Co-Authors: Dalibor Koutnik, Alzbeta Stara, Eliska Zuskova, Antonin Kouba, Josef VelisekAbstract:OBJECTIVES: The aim of the study was to investigate effects of the triazine herbicide metribuzin on signal crayfish Pacifastacus leniusculus Dana by determining oxidative stress (thiobarbituric acid reactive substances) and antioxidant indices (total superoxide dismutase, catalase, glutathione reductase) in Hepatopancreas, muscle, and gill as well as assessing their histopathology. DESIGN: Crayfish were exposed to metribuzin concentrations of 0.52 μg x l(-1) (realistic environmental concentration) and 3.06 mg x l(-1) (10% 96hLC50) for 10 and 30 days followed by a 30-day depuration period without exposure to metribuzin. RESULTS: In the thiobarbituric acid reactive substances, superoxide dismutase, and catalase were observed differences in all examined tissues compared to the control group. Differences from control were observed in glutathione reductase activity in Hepatopancreas after 10 days for both exposure concentrations and after 30 days at 3.06 mg x l(-1). Histological examination revealed extensive focal autolytic disintegration of tubular epithelium in Hepatopancreas of crayfish exposed to metribuzin for 30 days. CONCLUSIONS: Chronic exposure of metribuzin resulted in oxidative damage to cell lipids, in changes of antioxidant activity in crayfish tissue, and pathological changes in Hepatopancreas. The results suggest that selected oxidative stress biomarkers, antioxidant enzymes, and pathologies of Hepatopancreas may have potential as biomarkers for monitoring residual triazine herbicides in the aquatic environment.
