The Experts below are selected from a list of 6321 Experts worldwide ranked by ideXlab platform
Guijun Yan - One of the best experts on this subject based on the ideXlab platform.
-
qtl mapping using a high density genetic map to identify candidate genes associated with metribuzin Tolerance in hexaploid wheat triticum aestivum l
Frontiers in Plant Science, 2020Co-Authors: Hui Liu, Andrzej Kilian, Roopali Bhoite, Guannan Liu, Jing Wang, Weijun Zhou, Guijun YanAbstract:Tolerance to metribuzin, a broad-spectrum Herbicide, is an important trait for weed control in wheat breeding. However, the genetics of metribuzin Tolerance in relation to the underlying quantitative trait loci (QTL) and genes is limited. This study developed F8 recombinant inbred lines (RILs) from a cross between a highly resistant genotype (Chuan Mai 25) and highly susceptible genotype (Ritchie), which were used for QTL mapping of metribuzin Tolerance. Genotyping was done using a diversity arrays technology sequencing (DArTseq) platform, and phenotyping was done in controlled environments. Herbicide Tolerance was measured using three traits, visual score (VS), reduction of chlorophyll content (RCC), and mean value of chlorophyll content for metribuzin-treated plants (MCC). A high-density genetic linkage map was constructed using 2,129 DArTseq markers. Inclusive composite interval mapping (ICIM) identified seven QTL, one each on chromosomes 2A, 2D, 3A, 3B, 4A, 5A, and 6A. Three major QTL—Qrcc.uwa.2AS, Qrcc.uwa.5AL, and Qrcc.uwa.6AL—explained 11.39%, 11.06%, and 11.45% of the phenotypic variation, respectively. The 5A QTL was further validated using kompetitive allele-specific PCR (KASP) assays in an F3 validation population developed from Chuan Mai 25 × Dagger. Blasting the single nucleotide polymorphisms (SNPs) flanking the QTL in the wheat reference genome RefV1.0 revealed SNP markers within or very close to annotated genes which could be candidate genes responsible for metribuzin Tolerance. Most of the candidate genes were related to metabolic detoxification, especially those of P450 pathway and xenobiotic transmembrane transporter activity, which are reportedly key molecules responsible for Herbicide Tolerance. This study is the first to use specially developed populations to conduct QTL mapping on the metribuzin Tolerance trait. The three major QTL and candidate genes identified in this study could facilitate marker-assisted metribuzin breeding in wheat. The QTL could be fine-mapped to locate the genes responsible for metribuzin Tolerance, which could be introgressed into elite wheat cultivars.
-
identification and validation of qtl and their associated genes for pre emergent metribuzin Tolerance in hexaploid wheat triticum aestivum l
BMC Genetics, 2018Co-Authors: Roopali Bhoite, Ifeyinwa Onyemaobi, Kadambot H M Siddique, Guijun YanAbstract:Herbicide Tolerance is an important trait that allows effective weed management in wheat crops. Genetic knowledge of metribuzin Tolerance in wheat is needed to develop new cultivars for the industry. Here, we evaluated metribuzin Tolerance in a recombinant inbred line (RIL) mapping population derived from Synthetic W7984 and Opata 85 over two consecutive years to identify quantitative trait loci (QTL) contributing to the trait. Herbicide Tolerance was measured by two chlorophyll traits, SPAD chlorophyll content index (CCI) and visual senescence score (SNS). The markers associated with major QTL from Synthetic W7984, positively contributing to reduced phytotoxic effects under Herbicide treatment were validated in two F3/4 recombinant inbred populations developed from crosses of Synthetic W7984 × Westonia and Synthetic W7984 × Lang. Composite interval mapping (CIM) identified four QTL, two on chromosome 4A and one each on chromosomes 2D and 1A. The chromosomal position of the two QTL mapped on 4A within 10 cM intervals was refined and validated by multiple interval mapping (MIM). The major QTL affecting both measures of Tolerance jointly explained 42 and 45% of the phenotypic variation by percentage CCI reduction and SNS, respectively. The identified QTL have a pure additive effect. The metribuzin tolerant allele of markers, Xgwm33 and Xbarc343, conferred lower phytotoxicity and explained the maximum phenotypic variation of 28.8 and 24.5%, respectively. The approximate physical localization of the QTL revealed the presence of five candidate genes (ribulose-bisphosphate carboxylase, oxidoreductase (rbcS), glycosyltransferase, serine/threonine-specific protein kinase and phosphotransferase) with a direct role in photosynthesis and/or metabolic detoxification pathways. Metribuzin causes photo-inhibition by interrupting electron flow in PSII. Consequently, chlorophyll traits enabled the measure of high proportion of genetic variability in the mapping population. The validated molecular markers associated with metribuzin Tolerance mediating QTL may be used in marker-assisted breeding to select metribuzin tolerant lines. Alternatively, validated favourable alleles could be introgressed into elite wheat cultivars to enhance metribuzin Tolerance and improve grain yield in dryland farming for sustainable wheat production.
-
Identification and validation of QTL and their associated genes for pre-emergent metribuzin Tolerance in hexaploid wheat (Triticum aestivum L.)
BMC, 2018Co-Authors: Roopali Bhoite, Ifeyinwa Onyemaobi, Kadambot H M Siddique, Guijun YanAbstract:Abstract Background Herbicide Tolerance is an important trait that allows effective weed management in wheat crops. Genetic knowledge of metribuzin Tolerance in wheat is needed to develop new cultivars for the industry. Here, we evaluated metribuzin Tolerance in a recombinant inbred line (RIL) mapping population derived from Synthetic W7984 and Opata 85 over two consecutive years to identify quantitative trait loci (QTL) contributing to the trait. Herbicide Tolerance was measured by two chlorophyll traits, SPAD chlorophyll content index (CCI) and visual senescence score (SNS). The markers associated with major QTL from Synthetic W7984, positively contributing to reduced phytotoxic effects under Herbicide treatment were validated in two F3/4 recombinant inbred populations developed from crosses of Synthetic W7984 × Westonia and Synthetic W7984 × Lang. Results Composite interval mapping (CIM) identified four QTL, two on chromosome 4A and one each on chromosomes 2D and 1A. The chromosomal position of the two QTL mapped on 4A within 10 cM intervals was refined and validated by multiple interval mapping (MIM). The major QTL affecting both measures of Tolerance jointly explained 42 and 45% of the phenotypic variation by percentage CCI reduction and SNS, respectively. The identified QTL have a pure additive effect. The metribuzin tolerant allele of markers, Xgwm33 and Xbarc343, conferred lower phytotoxicity and explained the maximum phenotypic variation of 28.8 and 24.5%, respectively. The approximate physical localization of the QTL revealed the presence of five candidate genes (ribulose-bisphosphate carboxylase, oxidoreductase (rbcS), glycosyltransferase, serine/threonine-specific protein kinase and phosphotransferase) with a direct role in photosynthesis and/or metabolic detoxification pathways. Conclusion Metribuzin causes photo-inhibition by interrupting electron flow in PSII. Consequently, chlorophyll traits enabled the measure of high proportion of genetic variability in the mapping population. The validated molecular markers associated with metribuzin Tolerance mediating QTL may be used in marker-assisted breeding to select metribuzin tolerant lines. Alternatively, validated favourable alleles could be introgressed into elite wheat cultivars to enhance metribuzin Tolerance and improve grain yield in dryland farming for sustainable wheat production
-
genotypic variation of metribuzin and carfentrazone ethyl Tolerance among yellow lupin lupinus luteus l germplasm
New Zealand Journal of Crop and Horticultural Science, 2012Co-Authors: Guijun Yan, M A Kamsan, Kedar AdhikariAbstract:Yellow lupin (Lupinus luteus L.) is a high-protein grain legume growing well in a range of acid soil types. However, the lack of Herbicide Tolerance for effective weed control has limited its adoption in broad acre farming systems. In order to breed Herbicide-tolerant cultivars, a source of resistance needs to be identified. This paper reports the identification of a number of highly tolerant yellow lupin genotypes to metribuzin and carfentrazone-ethyl Herbicides. Suitable dosages (LD50) of the two Herbicides were identified as 150 g ai ha−1 (ai = active ingredient) for metribuzin and 200 g ai ha−1 for carfentrazone-ethyl. These dosages were used to screen diverse yellow lupin landraces and wild types to quantify the diversity of the germplasm. Considerable variation in response to the two Herbicides was observed among the genotypes screened, ranging from complete plant death to no symptoms. Yellow lupin cv. Pootallong was susceptible to metribuzin, but three accessions (P20873, P23291 and P28800) were id...
Swapan K. Datta - One of the best experts on this subject based on the ideXlab platform.
-
The development of Macrophomina phaseolina (fungus) resistant and glufosinate (Herbicide) tolerant transgenic jute.
Frontiers in plant science, 2018Co-Authors: Shuvobrata Majumder, Karabi Datta, Chirabrata Sarkar, Subhas C. Saha, Swapan K. DattaAbstract:The worldwide demand for natural bast fibres is met aptly by the long, golden and silky fibres of jute. This highest bast fibre producing crop is of great applicability and is extensively used in paper and textile industry. Macrophomina phaseolina (Tassi) Goid is a severely devastating necrotrophic fungal pathogen causing stem rot, root rot and charcoal rot diseases in both the cultivated species of jute – Corchorus capsularis and Corchorus olitorius. Another major problem faced in jute cultivation is profuse weed infestation in the fields. Huge losses in quality fibre production is caused by this pathogenic fungi and cultivation cost increases as well due to weed management expenditure during cropping season. To solve these long persisting jute cultivation challenges, the chitinase (chi11) gene (to provide fungus resistance) and the bar gene (to provide Herbicide Tolerance) have been incorporated in C. capsularis JRC-321 via Agrobacterium transformation and analyzed upto T2 generation. Stable integration and expression of these two genes in the jute genome was confirmed upon extensive analyses. Transgenic plants showed higher chitinase expression and chitin degrading activity than nontransgenic control plants. Antifungal activity significantly increased in transgenic plants as confirmed by detached leaf and whole plant M. phaseolina bioassay. Herbicide Tolerance was analyzed by growing transgenic plants in 10 mg/l glufosinate ammonium containing media and by spraying 0.25 % (v/v) glufosinate Herbicide Basta® on them. Assessment of residual phytotoxicity effects of Basta® on soil confirmed no negative impact on growth of indicator plants corn and cucumber. Transgenic jute plants were at par with non-transgenic (control) jute plants in all phenotypic aspects. Non-transgenic (control) jute plants suffered significant losses in fibre yield and quality due to M. phaseolina infection whereas the transgenic lines maintained the quality of fiber even after the infection.
-
Table_3_The Development of Macrophomina phaseolina (Fungus) Resistant and Glufosinate (Herbicide) Tolerant Transgenic Jute.pdf
2018Co-Authors: Shuvobrata Majumder, Karabi Datta, Chirabrata Sarkar, Subhas C. Saha, Swapan K. DattaAbstract:The worldwide demand for natural bast fibers is met aptly by the long, golden and silky fibers of jute. This highest bast fiber producing crop is of great applicability and is extensively used in paper and textile industry. Macrophomina phaseolina (Tassi) Goid is a severely devastating necrotrophic fungal pathogen causing stem rot, root rot, and charcoal rot diseases in both the cultivated species of jute – Corchorus capsularis and Corchorus olitorius. Another major problem faced in jute cultivation is profuse weed infestation in the fields. Huge losses in quality fiber production is caused by this pathogenic fungi and cultivation cost increases as well due to weed management expenditure during cropping season. To solve these long persisting jute cultivation challenges, the chitinase (chi11) gene (to provide fungus resistance) and the bar gene (to provide Herbicide Tolerance) have been incorporated in C. capsularis JRC-321 via Agrobacterium transformation and analyzed up to T2 generation. Stable integration and expression of these two genes in the jute genome was confirmed upon extensive analyses. Transgenic plants showed higher chitinase expression and chitin degrading activity than non-transgenic control plants. Antifungal activity significantly increased in transgenic plants as confirmed by detached leaf and whole plant M. phaseolina bioassay. Herbicide Tolerance was analyzed by growing transgenic plants in 10 mg/l glufosinate ammonium containing media and by spraying 0.25% (v/v) glufosinate Herbicide Basta® on them. Assessment of residual phytotoxicity effects of Basta® on soil confirmed no negative impact on growth of indicator plants corn and cucumber. Transgenic jute plants were at par with non-transgenic (control) jute plants in all phenotypic aspects. Non-transgenic (control) jute plants suffered significant losses in fiber yield and quality due to M. phaseolina infection whereas the transgenic lines maintained the quality of fiber even after the infection.
-
Image_1_The Development of Macrophomina phaseolina (Fungus) Resistant and Glufosinate (Herbicide) Tolerant Transgenic Jute.pdf
2018Co-Authors: Shuvobrata Majumder, Karabi Datta, Chirabrata Sarkar, Subhas C. Saha, Swapan K. DattaAbstract:The worldwide demand for natural bast fibers is met aptly by the long, golden and silky fibers of jute. This highest bast fiber producing crop is of great applicability and is extensively used in paper and textile industry. Macrophomina phaseolina (Tassi) Goid is a severely devastating necrotrophic fungal pathogen causing stem rot, root rot, and charcoal rot diseases in both the cultivated species of jute – Corchorus capsularis and Corchorus olitorius. Another major problem faced in jute cultivation is profuse weed infestation in the fields. Huge losses in quality fiber production is caused by this pathogenic fungi and cultivation cost increases as well due to weed management expenditure during cropping season. To solve these long persisting jute cultivation challenges, the chitinase (chi11) gene (to provide fungus resistance) and the bar gene (to provide Herbicide Tolerance) have been incorporated in C. capsularis JRC-321 via Agrobacterium transformation and analyzed up to T2 generation. Stable integration and expression of these two genes in the jute genome was confirmed upon extensive analyses. Transgenic plants showed higher chitinase expression and chitin degrading activity than non-transgenic control plants. Antifungal activity significantly increased in transgenic plants as confirmed by detached leaf and whole plant M. phaseolina bioassay. Herbicide Tolerance was analyzed by growing transgenic plants in 10 mg/l glufosinate ammonium containing media and by spraying 0.25% (v/v) glufosinate Herbicide Basta® on them. Assessment of residual phytotoxicity effects of Basta® on soil confirmed no negative impact on growth of indicator plants corn and cucumber. Transgenic jute plants were at par with non-transgenic (control) jute plants in all phenotypic aspects. Non-transgenic (control) jute plants suffered significant losses in fiber yield and quality due to M. phaseolina infection whereas the transgenic lines maintained the quality of fiber even after the infection.
Xiaoran Fu - One of the best experts on this subject based on the ideXlab platform.
-
development of enzymes for robust aryloxyphenoxypropionate and synthetic auxin Herbicide Tolerance traits in maize and soybean crops
Pest Management Science, 2019Co-Authors: Clayton T Larue, Michael Goley, Artem G Evdokimov, Oscar C Sparks, Christine M Ellis, Andrew M Wollacott, Coralie Halls, Brook Van Scoyoc, Timothy J. Rydel, Xiaoran FuAbstract:BACKGROUND: Effective management of weedy species in agricultural fields is essential for maintaining favorable growing conditions and crop yields. The introduction of genetically modified crops containing Herbicide Tolerance traits has been a successful additional tool available to farmers to better control weeds. However, weed resistance challenges present a need for additional Herbicide Tolerance trait options. RESULTS: To help meet this challenge, a new trait that provides Tolerance to an aryloxyphenoxypropionate (FOP) Herbicide and members of the synthetic auxin Herbicide family, such as 2,4-dichlorophenoxyacetic acid (2,4-D), was developed. Development of this Herbicide Tolerance trait employed an enzyme engineered with robust and specific enzymatic activity for these two Herbicide families. This engineering effort utilized a microbial-sourced dioxygenase scaffold to generate variants with improved enzymatic parameters. Additional optimization to enhance in-plant stability of the enzyme enabled an efficacious trait that can withstand the higher temperature conditions often found in field environments. CONCLUSION: Optimized Herbicide Tolerance enzyme variants with enhanced enzymatic and temperature stability parameters enabled robust Herbicide Tolerance for two Herbicide families in transgenic maize and soybeans. This Herbicide Tolerance trait for FOP and synthetic auxin Herbicides such as 2,4-D could be useful in weed management systems, providing additional tools for farmers to control weeds. © 2019 Society of Chemical Industry.
David S Douches - One of the best experts on this subject based on the ideXlab platform.
-
genome editing for crop improvement applications in clonally propagated polyploids with a focus on potato solanum tuberosum l
Frontiers in Plant Science, 2018Co-Authors: Satya Swathi Nadakuduti, Robin C Buell, Daniel F Voytas, Colby G Starker, David S DouchesAbstract:Genome-editing has revolutionized biology. When coupled with a recently streamlined regulatory process by the U.S. Department of Agriculture and the potential to generate transgene-free varieties, genome-editing provides a new avenue for crop improvement. For heterozygous, polyploid and vegetatively propagated crops such as cultivated potato, Solanum tuberosum Group Tuberosum L., genome-editing presents tremendous opportunities for trait improvement. In potato, traits such as improved resistance to cold-induced sweetening, processing efficiency, Herbicide Tolerance, modified starch quality and self-incompatibility have been targeted utilizing CRISPR/Cas9 and TALEN reagents in diploid and tetraploid clones. However, limited progress has been made in other such crops including sweetpotato, strawberry, grapes, citrus, banana etc., In this review we summarize the developments in genome-editing platforms, delivery mechanisms applicable to plants and then discuss the recent developments in regulation of genome-edited crops in the United States and The European Union. Next, we provide insight into the challenges of genome-editing in clonally propagated polyploid crops, their current status for trait improvement with future prospects focused on potato, a global food security crop.
-
genome editing for crop improvement applications in clonally propagated polyploids with a focus on potato solanum tuberosum l
Frontiers in Plant Science, 2018Co-Authors: Satya Swathi Nadakuduti, Robin C Buell, Daniel F Voytas, Colby G Starker, David S DouchesAbstract:Genome-editing has revolutionized biology. When coupled with a recently streamlined regulatory process by the U.S. Department of Agriculture and the potential to generate transgene-free varieties, genome-editing provides a new avenue for crop improvement. For heterozygous, polyploid and vegetatively propagated crops such as cultivated potato, Solanum tuberosum Group Tuberosum L., genome-editing presents tremendous opportunities for trait improvement. In potato, traits such as improved resistance to cold-induced sweetening, processing efficiency, Herbicide Tolerance, modified starch quality and self-incompatibility have been targeted utilizing CRISPR/Cas9 and TALEN reagents in diploid and tetraploid clones. However, limited progress has been made in other such crops including sweetpotato, strawberry, grapes, citrus, banana etc., In this review we summarize the developments in genome-editing platforms, delivery mechanisms applicable to plants and then discuss the recent developments in regulation of genome-edited crops in the U.S. and The European Union. Next, we provide insight into the challenges of genome-editing in clonally propagated polyploid crops, their current status for trait improvement with future prospects focused on potato, a global food security crop.
Shuvobrata Majumder - One of the best experts on this subject based on the ideXlab platform.
-
The development of Macrophomina phaseolina (fungus) resistant and glufosinate (Herbicide) tolerant transgenic jute.
Frontiers in plant science, 2018Co-Authors: Shuvobrata Majumder, Karabi Datta, Chirabrata Sarkar, Subhas C. Saha, Swapan K. DattaAbstract:The worldwide demand for natural bast fibres is met aptly by the long, golden and silky fibres of jute. This highest bast fibre producing crop is of great applicability and is extensively used in paper and textile industry. Macrophomina phaseolina (Tassi) Goid is a severely devastating necrotrophic fungal pathogen causing stem rot, root rot and charcoal rot diseases in both the cultivated species of jute – Corchorus capsularis and Corchorus olitorius. Another major problem faced in jute cultivation is profuse weed infestation in the fields. Huge losses in quality fibre production is caused by this pathogenic fungi and cultivation cost increases as well due to weed management expenditure during cropping season. To solve these long persisting jute cultivation challenges, the chitinase (chi11) gene (to provide fungus resistance) and the bar gene (to provide Herbicide Tolerance) have been incorporated in C. capsularis JRC-321 via Agrobacterium transformation and analyzed upto T2 generation. Stable integration and expression of these two genes in the jute genome was confirmed upon extensive analyses. Transgenic plants showed higher chitinase expression and chitin degrading activity than nontransgenic control plants. Antifungal activity significantly increased in transgenic plants as confirmed by detached leaf and whole plant M. phaseolina bioassay. Herbicide Tolerance was analyzed by growing transgenic plants in 10 mg/l glufosinate ammonium containing media and by spraying 0.25 % (v/v) glufosinate Herbicide Basta® on them. Assessment of residual phytotoxicity effects of Basta® on soil confirmed no negative impact on growth of indicator plants corn and cucumber. Transgenic jute plants were at par with non-transgenic (control) jute plants in all phenotypic aspects. Non-transgenic (control) jute plants suffered significant losses in fibre yield and quality due to M. phaseolina infection whereas the transgenic lines maintained the quality of fiber even after the infection.
-
Table_3_The Development of Macrophomina phaseolina (Fungus) Resistant and Glufosinate (Herbicide) Tolerant Transgenic Jute.pdf
2018Co-Authors: Shuvobrata Majumder, Karabi Datta, Chirabrata Sarkar, Subhas C. Saha, Swapan K. DattaAbstract:The worldwide demand for natural bast fibers is met aptly by the long, golden and silky fibers of jute. This highest bast fiber producing crop is of great applicability and is extensively used in paper and textile industry. Macrophomina phaseolina (Tassi) Goid is a severely devastating necrotrophic fungal pathogen causing stem rot, root rot, and charcoal rot diseases in both the cultivated species of jute – Corchorus capsularis and Corchorus olitorius. Another major problem faced in jute cultivation is profuse weed infestation in the fields. Huge losses in quality fiber production is caused by this pathogenic fungi and cultivation cost increases as well due to weed management expenditure during cropping season. To solve these long persisting jute cultivation challenges, the chitinase (chi11) gene (to provide fungus resistance) and the bar gene (to provide Herbicide Tolerance) have been incorporated in C. capsularis JRC-321 via Agrobacterium transformation and analyzed up to T2 generation. Stable integration and expression of these two genes in the jute genome was confirmed upon extensive analyses. Transgenic plants showed higher chitinase expression and chitin degrading activity than non-transgenic control plants. Antifungal activity significantly increased in transgenic plants as confirmed by detached leaf and whole plant M. phaseolina bioassay. Herbicide Tolerance was analyzed by growing transgenic plants in 10 mg/l glufosinate ammonium containing media and by spraying 0.25% (v/v) glufosinate Herbicide Basta® on them. Assessment of residual phytotoxicity effects of Basta® on soil confirmed no negative impact on growth of indicator plants corn and cucumber. Transgenic jute plants were at par with non-transgenic (control) jute plants in all phenotypic aspects. Non-transgenic (control) jute plants suffered significant losses in fiber yield and quality due to M. phaseolina infection whereas the transgenic lines maintained the quality of fiber even after the infection.
-
Image_1_The Development of Macrophomina phaseolina (Fungus) Resistant and Glufosinate (Herbicide) Tolerant Transgenic Jute.pdf
2018Co-Authors: Shuvobrata Majumder, Karabi Datta, Chirabrata Sarkar, Subhas C. Saha, Swapan K. DattaAbstract:The worldwide demand for natural bast fibers is met aptly by the long, golden and silky fibers of jute. This highest bast fiber producing crop is of great applicability and is extensively used in paper and textile industry. Macrophomina phaseolina (Tassi) Goid is a severely devastating necrotrophic fungal pathogen causing stem rot, root rot, and charcoal rot diseases in both the cultivated species of jute – Corchorus capsularis and Corchorus olitorius. Another major problem faced in jute cultivation is profuse weed infestation in the fields. Huge losses in quality fiber production is caused by this pathogenic fungi and cultivation cost increases as well due to weed management expenditure during cropping season. To solve these long persisting jute cultivation challenges, the chitinase (chi11) gene (to provide fungus resistance) and the bar gene (to provide Herbicide Tolerance) have been incorporated in C. capsularis JRC-321 via Agrobacterium transformation and analyzed up to T2 generation. Stable integration and expression of these two genes in the jute genome was confirmed upon extensive analyses. Transgenic plants showed higher chitinase expression and chitin degrading activity than non-transgenic control plants. Antifungal activity significantly increased in transgenic plants as confirmed by detached leaf and whole plant M. phaseolina bioassay. Herbicide Tolerance was analyzed by growing transgenic plants in 10 mg/l glufosinate ammonium containing media and by spraying 0.25% (v/v) glufosinate Herbicide Basta® on them. Assessment of residual phytotoxicity effects of Basta® on soil confirmed no negative impact on growth of indicator plants corn and cucumber. Transgenic jute plants were at par with non-transgenic (control) jute plants in all phenotypic aspects. Non-transgenic (control) jute plants suffered significant losses in fiber yield and quality due to M. phaseolina infection whereas the transgenic lines maintained the quality of fiber even after the infection.