The Experts below are selected from a list of 10482 Experts worldwide ranked by ideXlab platform
Venugopal P. Menon - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of antioxidant activity of Hesperidin and its protective effect on H_2O_2 induced oxidative damage on pBR322 DNA and RBC cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of Hesperidin and to study its protective effect on H_2O_2 induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of Hesperidin and the results clearly indicate that Hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of Hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H_2O_2 shows that Hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves Hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
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evaluation of antioxidant activity of Hesperidin and its protective effect on h2o2 induced oxidative damage on pbr322 dna and rbc cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of Hesperidin and to study its protective effect on H2O2 induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of Hesperidin and the results clearly indicate that Hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of Hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H2O2 shows that Hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves Hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
K. B. Kalpana - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of antioxidant activity of Hesperidin and its protective effect on H_2O_2 induced oxidative damage on pBR322 DNA and RBC cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of Hesperidin and to study its protective effect on H_2O_2 induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of Hesperidin and the results clearly indicate that Hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of Hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H_2O_2 shows that Hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves Hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
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evaluation of antioxidant activity of Hesperidin and its protective effect on h2o2 induced oxidative damage on pbr322 dna and rbc cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of Hesperidin and to study its protective effect on H2O2 induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of Hesperidin and the results clearly indicate that Hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of Hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H2O2 shows that Hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves Hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
Matthew W. Bernart - One of the best experts on this subject based on the ideXlab platform.
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High-performance liquid chromatography–electrospray mass spectrometry in phytochemical analysis of sour orange (Citrus aurantium L.)
Journal of Chromatography A, 1997Co-Authors: Xian-guo He, Li-zhi Lian, Matthew W. BernartAbstract:Abstract High-performance liquid chromatography coupled with a UV photodiode-array detector and an electrospray mass spectrometer (HPLC–ES-MS) was used to analyze the phytochemical constituents of an extract of sour orange ( Citrus aurantium L., family Rutaceae). Eight flavonoids were identified, as isonaringin ( 1 ), naringin ( 3 ), Hesperidin ( 5 ), neoHesperidin ( 6 ), naringenin ( 2 ), hesperitin ( 4 ), nobiletin ( 8 ) and tangeritin ( 7 ). The permethoxylated flavones tangeritin ( 7 ) and nobiletin ( 8 ) showed the most intense protonated molecules. This method can be used to detect trace amounts of these compounds in an orange extract.
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high performance liquid chromatography electrospray mass spectrometry in phytochemical analysis of sour orange citrus aurantium l
Journal of Chromatography A, 1997Co-Authors: Li-zhi Lian, Longze Lin, Matthew W. BernartAbstract:Abstract High-performance liquid chromatography coupled with a UV photodiode-array detector and an electrospray mass spectrometer (HPLC–ES-MS) was used to analyze the phytochemical constituents of an extract of sour orange ( Citrus aurantium L., family Rutaceae). Eight flavonoids were identified, as isonaringin ( 1 ), naringin ( 3 ), Hesperidin ( 5 ), neoHesperidin ( 6 ), naringenin ( 2 ), hesperitin ( 4 ), nobiletin ( 8 ) and tangeritin ( 7 ). The permethoxylated flavones tangeritin ( 7 ) and nobiletin ( 8 ) showed the most intense protonated molecules. This method can be used to detect trace amounts of these compounds in an orange extract.
M. Srinivasan - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of antioxidant activity of Hesperidin and its protective effect on H_2O_2 induced oxidative damage on pBR322 DNA and RBC cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of Hesperidin and to study its protective effect on H_2O_2 induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of Hesperidin and the results clearly indicate that Hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of Hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H_2O_2 shows that Hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves Hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
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evaluation of antioxidant activity of Hesperidin and its protective effect on h2o2 induced oxidative damage on pbr322 dna and rbc cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of Hesperidin and to study its protective effect on H2O2 induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of Hesperidin and the results clearly indicate that Hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of Hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H2O2 shows that Hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves Hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
Li-zhi Lian - One of the best experts on this subject based on the ideXlab platform.
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High-performance liquid chromatography–electrospray mass spectrometry in phytochemical analysis of sour orange (Citrus aurantium L.)
Journal of Chromatography A, 1997Co-Authors: Xian-guo He, Li-zhi Lian, Matthew W. BernartAbstract:Abstract High-performance liquid chromatography coupled with a UV photodiode-array detector and an electrospray mass spectrometer (HPLC–ES-MS) was used to analyze the phytochemical constituents of an extract of sour orange ( Citrus aurantium L., family Rutaceae). Eight flavonoids were identified, as isonaringin ( 1 ), naringin ( 3 ), Hesperidin ( 5 ), neoHesperidin ( 6 ), naringenin ( 2 ), hesperitin ( 4 ), nobiletin ( 8 ) and tangeritin ( 7 ). The permethoxylated flavones tangeritin ( 7 ) and nobiletin ( 8 ) showed the most intense protonated molecules. This method can be used to detect trace amounts of these compounds in an orange extract.
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high performance liquid chromatography electrospray mass spectrometry in phytochemical analysis of sour orange citrus aurantium l
Journal of Chromatography A, 1997Co-Authors: Li-zhi Lian, Longze Lin, Matthew W. BernartAbstract:Abstract High-performance liquid chromatography coupled with a UV photodiode-array detector and an electrospray mass spectrometer (HPLC–ES-MS) was used to analyze the phytochemical constituents of an extract of sour orange ( Citrus aurantium L., family Rutaceae). Eight flavonoids were identified, as isonaringin ( 1 ), naringin ( 3 ), Hesperidin ( 5 ), neoHesperidin ( 6 ), naringenin ( 2 ), hesperitin ( 4 ), nobiletin ( 8 ) and tangeritin ( 7 ). The permethoxylated flavones tangeritin ( 7 ) and nobiletin ( 8 ) showed the most intense protonated molecules. This method can be used to detect trace amounts of these compounds in an orange extract.