The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Jan Stenlid - One of the best experts on this subject based on the ideXlab platform.
-
Spread of Heterobasidion Annosum s.s. and Heterobasidion parviporum in Picea abies 15 years after stump inoculation.
FEMS microbiology ecology, 2011Co-Authors: Jonàs Oliva, Malin Bendz-hellgren, Jan StenlidAbstract:The tree pathogenic fungi Heterobasidion Annosum s.s. and Heterobasidion parviporum cause root and butt rot in Norway spruce (Picea abies) and produce serious economic losses to the forest sector in Europe. We experimentally studied inter- and intraspecific differences between H. parviporum and H. Annosum s.s. in the way they infect stumps and spread into neighbouring trees. Eleven H. parviporum and nine H. Annosum s.s. isolates were artificially inoculated on stumps of two spruce stands after first thinning. After 15 years, the same isolates were reisolated from neighbouring trees. Heterobasidion parviporum spread more frequently from the inoculated stumps to the neighbouring trees than H. Annosum s.s. The surroundings of H. Annosum s.s. stumps that did not spread were often colonized by H. parviporum. Heterobasidion Annosum s.s. spread was restricted mainly to the areas of the plot where no other Heterobasidion genotypes had been inoculated. In such cases, H. Annosum s.s. tended to develop into bigger genets than H. parviporum. The probability of stump-to-tree spread of H. parviporum depended on the diameter of the stumps, suggesting that H. parviporum spread may relate to the presence of heartwood. Both H. parviporum and H. Annosum s.s. proved to be strong pathogens on Norway spruce; however, when competing for the same trees, H. parviporum seemed capable of excluding H. Annosum s.s. from the stand.
-
Two hydrophobin genes from the conifer pathogen Heterobasidion Annosum are expressed in aerial hyphae.
Mycologia, 2007Co-Authors: Magnus Karlsson, Jan Stenlid, Åke OlsonAbstract:Two hydrophobin genes (HAH1 and HAH2) have been identified in a Heterobasidion Annosum infection-stage cDNA-library. Comparisons of their nucleotide and amino acid sequences show similarity to the coh1 hydrophobin from Coprinopsis cinerea and the sc3 hydrophobin from Schizophyllum commune. Both HAH1 and HAH2 display the amino acid consensus pattern of class I hydrophobins, including the spacing of eight conserved cysteine residues. Real-time quantitative RT-PCR showed high expression of both genes in aerial hyphae but low expression in submerged hyphae and during in vitro infection of pine seedlings. Segregation analysis of HAH1 and HAH2 in a defined cross of Heterobasidion Annosum localised HAH1 to linkage group 3 but did not positioned HAH2 in the genetic linkage map. Sequence characteristics and expression patterns of HAH1 and HAH2 suggest a role in aerial growth of mycelia, but not during pathogenesis.
-
development of a rapid and simple agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion Annosum
Fems Microbiology Letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Development of a rapid and simple Agrobacterium tumefaciens‐mediated transformation system for the fungal pathogen Heterobasidion Annosum
FEMS microbiology letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Identification of a superoxide dismutase gene from the conifer pathogen Heterobasidion Annosum
Physiological and Molecular Plant Pathology, 2005Co-Authors: Magnus Karlsson, Jan Stenlid, Åke OlsonAbstract:Identification of a superoxide dismutase gene from the conifer pathogen Heterobasidion Annosum.
Daniel L. Czederpiltz - One of the best experts on this subject based on the ideXlab platform.
-
development of a rapid and simple agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion Annosum
Fems Microbiology Letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Development of a rapid and simple Agrobacterium tumefaciens‐mediated transformation system for the fungal pathogen Heterobasidion Annosum
FEMS microbiology letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
Nicklas Samils - One of the best experts on this subject based on the ideXlab platform.
-
development of a rapid and simple agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion Annosum
Fems Microbiology Letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Development of a rapid and simple Agrobacterium tumefaciens‐mediated transformation system for the fungal pathogen Heterobasidion Annosum
FEMS microbiology letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
Malin Elfstrand - One of the best experts on this subject based on the ideXlab platform.
-
development of a rapid and simple agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion Annosum
Fems Microbiology Letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Development of a rapid and simple Agrobacterium tumefaciens‐mediated transformation system for the fungal pathogen Heterobasidion Annosum
FEMS microbiology letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Differential gene expression during interactions between Heterobasidion Annosum and Physisporinus sanguinolentus
FEMS microbiology letters, 2004Co-Authors: Andrei Iakovlev, Malin Elfstrand, Åke Olson, Jan StenlidAbstract:Using mRNA differential display we have identified differentially expressed genes in non-self-interacting vs. single mycelia of the conifer pathogen Heterobasidion Annosum and the wood decomposing basidiomycete Physisporinus sanguinolentus. Altogether 39 differentially displayed bands were cloned and sequenced, corresponding to 21 unique genes, which were confirmed by semi-quantitative RT-PCR to be differentially expressed. Further confirmation of differential gene expression was made by real time RT-PCR. All 10 genes identified from P. sanguinolentus had lower expression, while in H. Annosum three genes had higher and eight lower expression in non-self-interacting mycelia vs. single mycelia. One of the induced genes showed high similarity to the Coprinus cinereus recA/RAD51 homolog (rah1) which is essential for homologous recombination, DNA repair and stress responses.
Åke Olson - One of the best experts on this subject based on the ideXlab platform.
-
Two hydrophobin genes from the conifer pathogen Heterobasidion Annosum are expressed in aerial hyphae.
Mycologia, 2007Co-Authors: Magnus Karlsson, Jan Stenlid, Åke OlsonAbstract:Two hydrophobin genes (HAH1 and HAH2) have been identified in a Heterobasidion Annosum infection-stage cDNA-library. Comparisons of their nucleotide and amino acid sequences show similarity to the coh1 hydrophobin from Coprinopsis cinerea and the sc3 hydrophobin from Schizophyllum commune. Both HAH1 and HAH2 display the amino acid consensus pattern of class I hydrophobins, including the spacing of eight conserved cysteine residues. Real-time quantitative RT-PCR showed high expression of both genes in aerial hyphae but low expression in submerged hyphae and during in vitro infection of pine seedlings. Segregation analysis of HAH1 and HAH2 in a defined cross of Heterobasidion Annosum localised HAH1 to linkage group 3 but did not positioned HAH2 in the genetic linkage map. Sequence characteristics and expression patterns of HAH1 and HAH2 suggest a role in aerial growth of mycelia, but not during pathogenesis.
-
development of a rapid and simple agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion Annosum
Fems Microbiology Letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Development of a rapid and simple Agrobacterium tumefaciens‐mediated transformation system for the fungal pathogen Heterobasidion Annosum
FEMS microbiology letters, 2006Co-Authors: Nicklas Samils, Åke Olson, Malin Elfstrand, Daniel L. Czederpiltz, Jan Fahleson, Christina Dixelius, Jan StenlidAbstract:Heterobasidion Annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. Annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion Annosum TC 32-1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
-
Identification of a superoxide dismutase gene from the conifer pathogen Heterobasidion Annosum
Physiological and Molecular Plant Pathology, 2005Co-Authors: Magnus Karlsson, Jan Stenlid, Åke OlsonAbstract:Identification of a superoxide dismutase gene from the conifer pathogen Heterobasidion Annosum.
-
Differential gene expression during interactions between Heterobasidion Annosum and Physisporinus sanguinolentus
FEMS microbiology letters, 2004Co-Authors: Andrei Iakovlev, Malin Elfstrand, Åke Olson, Jan StenlidAbstract:Using mRNA differential display we have identified differentially expressed genes in non-self-interacting vs. single mycelia of the conifer pathogen Heterobasidion Annosum and the wood decomposing basidiomycete Physisporinus sanguinolentus. Altogether 39 differentially displayed bands were cloned and sequenced, corresponding to 21 unique genes, which were confirmed by semi-quantitative RT-PCR to be differentially expressed. Further confirmation of differential gene expression was made by real time RT-PCR. All 10 genes identified from P. sanguinolentus had lower expression, while in H. Annosum three genes had higher and eight lower expression in non-self-interacting mycelia vs. single mycelia. One of the induced genes showed high similarity to the Coprinus cinereus recA/RAD51 homolog (rah1) which is essential for homologous recombination, DNA repair and stress responses.