The Experts below are selected from a list of 47223 Experts worldwide ranked by ideXlab platform
Petri Auvinen - One of the best experts on this subject based on the ideXlab platform.
-
Lactobacillus oligofermentans glucose, ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism-induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Background Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose.
-
lactobacillus oligofermentans glucose ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose. The genome was manually annotated and its main functional features were examined. L. oligofermentans was confirmed to be able to efficiently utilize several Hexoses and maltose, which is, presumably, induced by its repeated cultivation with glucose in vitro. Unexpectedly, in the beginning of the exponential growth phase, glucose- and xylose-induced transcriptome responses were more similar, whereas toward the end of the growth phase xylose and ribose transcriptomes became more alike. The promoter regions of genes simultaneously upregulated both on glucose and xylose in comparison with ribose (particularly, Hexose and xylose utilization genes) were found to be enriched in the CcpA- binding site. Transcriptionally, no glucose-induced carbon catabolite repression was detected. The catabolism of glucose, which requires initial oxidation, led to significant overexpression of the NAD(P)H re-oxidation genes, the upstream regions of which were found to contain a motif, which was highly similar to a Rex repressor binding site. This paper presents the second complete genome and the first study of carbohydrate catabolism-dependent transcriptome response for a member of the L. vaccinostercus group. The transcriptomic changes detected in L. oligofermentans for growth with different carbohydrates differ significantly from those of facultative heterofermentative lactobacilli. The mechanism of CcpA regulation, putatively contributing to the observed similarities between glucose- and xylose-induced transcriptome responses and the absence of stringent carbon catabolite control, requires further studies. Finally, the cell redox balance maintenance, in terms of the NAD(P)+/NAD(P)H ratio, was predicted to be regulated by the Rex transcriptional regulator, supporting the previously made inference of Rex-regulons for members of the Lactobacillaceae family.
Jarmo Ritari - One of the best experts on this subject based on the ideXlab platform.
-
Lactobacillus oligofermentans glucose, ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism-induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Background Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose.
-
lactobacillus oligofermentans glucose ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose. The genome was manually annotated and its main functional features were examined. L. oligofermentans was confirmed to be able to efficiently utilize several Hexoses and maltose, which is, presumably, induced by its repeated cultivation with glucose in vitro. Unexpectedly, in the beginning of the exponential growth phase, glucose- and xylose-induced transcriptome responses were more similar, whereas toward the end of the growth phase xylose and ribose transcriptomes became more alike. The promoter regions of genes simultaneously upregulated both on glucose and xylose in comparison with ribose (particularly, Hexose and xylose utilization genes) were found to be enriched in the CcpA- binding site. Transcriptionally, no glucose-induced carbon catabolite repression was detected. The catabolism of glucose, which requires initial oxidation, led to significant overexpression of the NAD(P)H re-oxidation genes, the upstream regions of which were found to contain a motif, which was highly similar to a Rex repressor binding site. This paper presents the second complete genome and the first study of carbohydrate catabolism-dependent transcriptome response for a member of the L. vaccinostercus group. The transcriptomic changes detected in L. oligofermentans for growth with different carbohydrates differ significantly from those of facultative heterofermentative lactobacilli. The mechanism of CcpA regulation, putatively contributing to the observed similarities between glucose- and xylose-induced transcriptome responses and the absence of stringent carbon catabolite control, requires further studies. Finally, the cell redox balance maintenance, in terms of the NAD(P)+/NAD(P)H ratio, was predicted to be regulated by the Rex transcriptional regulator, supporting the previously made inference of Rex-regulons for members of the Lactobacillaceae family.
Tanja Rämö - One of the best experts on this subject based on the ideXlab platform.
-
Lactobacillus oligofermentans glucose, ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism-induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Background Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose.
-
lactobacillus oligofermentans glucose ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose. The genome was manually annotated and its main functional features were examined. L. oligofermentans was confirmed to be able to efficiently utilize several Hexoses and maltose, which is, presumably, induced by its repeated cultivation with glucose in vitro. Unexpectedly, in the beginning of the exponential growth phase, glucose- and xylose-induced transcriptome responses were more similar, whereas toward the end of the growth phase xylose and ribose transcriptomes became more alike. The promoter regions of genes simultaneously upregulated both on glucose and xylose in comparison with ribose (particularly, Hexose and xylose utilization genes) were found to be enriched in the CcpA- binding site. Transcriptionally, no glucose-induced carbon catabolite repression was detected. The catabolism of glucose, which requires initial oxidation, led to significant overexpression of the NAD(P)H re-oxidation genes, the upstream regions of which were found to contain a motif, which was highly similar to a Rex repressor binding site. This paper presents the second complete genome and the first study of carbohydrate catabolism-dependent transcriptome response for a member of the L. vaccinostercus group. The transcriptomic changes detected in L. oligofermentans for growth with different carbohydrates differ significantly from those of facultative heterofermentative lactobacilli. The mechanism of CcpA regulation, putatively contributing to the observed similarities between glucose- and xylose-induced transcriptome responses and the absence of stringent carbon catabolite control, requires further studies. Finally, the cell redox balance maintenance, in terms of the NAD(P)+/NAD(P)H ratio, was predicted to be regulated by the Rex transcriptional regulator, supporting the previously made inference of Rex-regulons for members of the Lactobacillaceae family.
Margarita Andreevskaya - One of the best experts on this subject based on the ideXlab platform.
-
Lactobacillus oligofermentans glucose, ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism-induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Background Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose.
-
lactobacillus oligofermentans glucose ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism induced responses in the early exponential growth phase
BMC Genomics, 2016Co-Authors: Margarita Andreevskaya, Tanja Rämö, Johanna Björkroth, Jarmo Ritari, Elina Jaaskelainen, Per Johansson, Lars Paulin, Petri AuvinenAbstract:Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than Hexoses (e.g. glucose). However, more efficient Hexose utilization can be induced. The regulation mechanisms of the carbohydrate catabolism in such bacteria have been scarcely studied. To address this question, we provided the complete genome sequence of L. oligofermentans LMG 22743T and generated time course transcriptomes during its growth on glucose, ribose and xylose. The genome was manually annotated and its main functional features were examined. L. oligofermentans was confirmed to be able to efficiently utilize several Hexoses and maltose, which is, presumably, induced by its repeated cultivation with glucose in vitro. Unexpectedly, in the beginning of the exponential growth phase, glucose- and xylose-induced transcriptome responses were more similar, whereas toward the end of the growth phase xylose and ribose transcriptomes became more alike. The promoter regions of genes simultaneously upregulated both on glucose and xylose in comparison with ribose (particularly, Hexose and xylose utilization genes) were found to be enriched in the CcpA- binding site. Transcriptionally, no glucose-induced carbon catabolite repression was detected. The catabolism of glucose, which requires initial oxidation, led to significant overexpression of the NAD(P)H re-oxidation genes, the upstream regions of which were found to contain a motif, which was highly similar to a Rex repressor binding site. This paper presents the second complete genome and the first study of carbohydrate catabolism-dependent transcriptome response for a member of the L. vaccinostercus group. The transcriptomic changes detected in L. oligofermentans for growth with different carbohydrates differ significantly from those of facultative heterofermentative lactobacilli. The mechanism of CcpA regulation, putatively contributing to the observed similarities between glucose- and xylose-induced transcriptome responses and the absence of stringent carbon catabolite control, requires further studies. Finally, the cell redox balance maintenance, in terms of the NAD(P)+/NAD(P)H ratio, was predicted to be regulated by the Rex transcriptional regulator, supporting the previously made inference of Rex-regulons for members of the Lactobacillaceae family.
Eckhard Boles - One of the best experts on this subject based on the ideXlab platform.
-
arsenic trioxide uptake by Hexose permeases in saccharomyces cerevisiae
Journal of Biological Chemistry, 2004Co-Authors: Zijuan Liu, Eckhard Boles, Barry P. RosenAbstract:Arsenic trioxide is a toxic metalloid and carcinogen that is also used as an anticancer drug, and for this reason it is important to identify the routes of arsenite uptake by cells. In this study the ability of Hexose transporters to facilitate arsenic trioxide uptake in Saccharomyces cerevisiae was examined. In the absence of glucose, strains with disruption of the arsenite efflux gene ACR3 accumulated high levels of (73)As(OH)(3). The addition of glucose inhibited uptake by approximately 80%. Disruption of FPS1, the aquaglyceroporin gene, reduced glucose-independent uptake by only about 25%, and the residual uptake was nearly completely inhibited by Hexoses, including glucose, galactose, mannose, and fructose but not pentoses or disaccharides. A strain lacking FPS1, ACR3, and all genes for Hexose permeases except for HXT3, HXT6, HXT7, and GAL2 exhibited Hexose-inhibitable (73)As(OH)(3) uptake, whereas a strain lacking all 18 Hexose transport-related genes (HXT1 to HXT17 and GAL2), FPS1 and ACR3, exhibited <10% of wild type (73)As(OH)(3) transport. When HXT1, HXT3, HXT4, HXT5, HXT7, or HXT9 was individually expressed in that strain, Hexose-inhibitable (73)As(OH)(3) uptake was restored. In addition, the transport of [(14)C]glucose was inhibited by As(OH)(3). These results clearly demonstrate that Hexose permeases catalyze the majority of the transport of the trivalent metalloid arsenic trioxide.
-
Arsenic trioxide uptake by Hexose permeases in Saccharomyces cerevisiae.
The Journal of biological chemistry, 2004Co-Authors: Zijuan Liu, Eckhard Boles, Barry P. RosenAbstract:Arsenic trioxide is a toxic metalloid and carcinogen that is also used as an anticancer drug, and for this reason it is important to identify the routes of arsenite uptake by cells. In this study the ability of Hexose transporters to facilitate arsenic trioxide uptake in Saccharomyces cerevisiae was examined. In the absence of glucose, strains with disruption of the arsenite efflux gene ACR3 accumulated high levels of (73)As(OH)(3). The addition of glucose inhibited uptake by approximately 80%. Disruption of FPS1, the aquaglyceroporin gene, reduced glucose-independent uptake by only about 25%, and the residual uptake was nearly completely inhibited by Hexoses, including glucose, galactose, mannose, and fructose but not pentoses or disaccharides. A strain lacking FPS1, ACR3, and all genes for Hexose permeases except for HXT3, HXT6, HXT7, and GAL2 exhibited Hexose-inhibitable (73)As(OH)(3) uptake, whereas a strain lacking all 18 Hexose transport-related genes (HXT1 to HXT17 and GAL2), FPS1 and ACR3, exhibited
-
concurrent knock out of at least 20 transporter genes is required to block uptake of Hexoses in saccharomyces cerevisiae
FEBS Letters, 1999Co-Authors: Roman Wieczorke, Stefanie Krampe, Thomas Weierstall, Kerstin Freidel, Cornelis P Hollenberg, Eckhard BolesAbstract:The Hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1–17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport Hexoses. In a yeast strain deleted for HXT1–17, GAL2, AGT1, YDL247w and YJR160c, glucose consumption and transport activity were completely abolished. However, as additional deletion of the glucose sensor gene SNF3 partially restored growth on Hexoses, our data indicate the existence of even more proteins able to transport Hexoses in yeast.
-
The molecular genetics of Hexose transport in yeasts
FEMS microbiology reviews, 1997Co-Authors: Eckhard Boles, Cornelis P HollenbergAbstract:Transport across the plasma membrane is the first, obligatory step of Hexose utilization. In yeast cells the uptake of Hexoses is mediated by a large family of related transporter proteins. In baker's yeast Saccharomyces cerevisiae the genes of 20 different Hexose transporter-related proteins have been identified. Six of these transmembrane proteins mediate the metabolically relevant uptake of glucose, fructose and mannose for growth, two others catalyze the transport of only small amounts of these sugars, one protein is a galactose transporter but also able to transport glucose, two transporters act as glucose sensors, two others are involved in the pleiotropic drug resistance process, and the functions of the remaining Hexose transporter-related proteins are not yet known. The catabolic Hexose transporters exhibit different affinities for their substrates, and expression of their corresponding genes is controlled by the glucose sensors according to the availability of carbon sources. In contrast, milk yeast Kluyveromyces lactis contains only a few different Hexose transporters. Genes of other monosaccharide transporter-related proteins have been found in fission yeast Schizosaccharomyces pombe and in the xylose-fermenting yeast Pichia stipitis. However, the molecular genetics of Hexose transport in many other yeasts remains to be established. The further characterization of this multigene family of Hexose transporters should help to elucidate the role of transport in yeast sugar metabolism.