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Millogo Nicolas Alfred - One of the best experts on this subject based on the ideXlab platform.
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Structure démographique et mouvements saisonniers des populations d'hippopotame commun, Hippopotamus amphibius Linné 1758 dans la zone sud soudanienne du Burkina faso:
Tropical Conservation Science, 2010Co-Authors: Théophile Ollo Dibloni, Cédric Vermeulen, Wendengoudi Guenda, Millogo Nicolas AlfredAbstract:The study was conducted in the “Mare aux Hippopotames” Biosphere Reserve of Burkina Faso and aimed at assessing the number, age structures and seasonal mobility of Hippopotamus amphibius. Methodology used was based on three consecutive-year (2006-2008) monitoring and ground inventory inside and outside the reserve. The results of the inventories have led to the identification of 41 Hippopotamus in 2008 versus 35 in 2006 which are divided into 3 distinct herds. The structure in age group of that population was composed of 32 were adults, 5 sub-adults and 4 juveniles. Location of places where they take a rest varies with the level of the water in the pond. The inventory has identified eight sites on each side of the pond as used by hippos to harvest food. In addition, exploration of the reserve has identified four temporary ponds located near the water meadows used by hippos during the rainy season. These areas were surrounded by agricultural fields that were often raided by these mammals for food. It is urgent to establish agricultural zoning and to grant a special conservation status to the four additional ponds to minimize conflicts between humans and Hippopotamus. Finally, local participation in conservation activities is probably greatly responsible for an increase in the size of the hippo population.
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Structure démographique et mouvements saisonniers des populations d’hippopotame commun, Hippopotamus amphibius Linné 1758 dans la Réserve de Biosphère de la Mare aux Hippopotames en zone sud soudanienne du Burkina Faso
Mongabay.com Open Access Journal, 2010Co-Authors: Dibloni, Ollo Théophile, Vermeulen Cédric, Guenda Wendengoudi, Millogo Nicolas AlfredAbstract:peer reviewedaudience: researcher, professional, student, popularizationL’étude de l’effectif, de la structure en classes d’âges et des mouvements de Hippopotamus amphibius a été conduite dans la Réserve de Biosphère de la Mare aux Hippopotames au Burkina Faso. Pendant 3 années d’affilée (2006, 2007 et 2008), des prospections et des inventaires de terrain ont été menés à l’intérieur et à la lisière de la réserve. Les résultats des inventaires ont permis de dénombrer 41 hippopotames en 2008 contre 35 têtes en 2006, répartis en 3 troupeaux distincts. La structure en classes d’âges de cette population était de 32 adultes, 5 subadultes et 4 juvéniles. L’emplacement de leurs aires de repos dans la mare variait suivant le niveau de l’eau. L’inventaire a identifié 8 sites de sorties, sur chaque rive de la mare, utilisées par les hippopotames pour se rendre dans les gagnages. De même, la prospection de la réserve a identifié 4 mares temporaires situées à proximité des prairies aquatiques qui constituaient des zones de migration où les hippopotames se réfugiaient pendant la saison pluvieuse. Ces zones étaient entourées par des champs qui étaient souvent saccagés par ces mammifères pour leur alimentation. Il est urgent de négocier un zonage de l’activité agricole et de conférer un statut particulier de conservation à ces 4 sites afin de minimiser les conflits homme-hippopotame. Les récentes actions de surveillance menées en partenariat avec les communautés locales ont probablement permis l’accroissement de l’effectif de 6 hippopotames en 3 ans.The study was conducted in the “Mare aux Hippopotames” Biosphere Reserve of Burkina Faso and aimed at assessing the number, age structures and seasonal mobility of Hippopotamus amphibius. Methodology used was based on three consecutive-year (2006-2008) monitoring and ground inventory inside and outside the reserve. The results of the inventories have led to the identification of 41 Hippopotamus in 2008 versus 35 in 2006 which are divided into 3 distinct herds. The structure in age group of that population was composed of 32 were adults, 5 sub-adults and 4 juveniles. Location of places where they take a rest varies with the level of the water in the pond. The inventory has identified eight sites on each side of the pond as used by hippos to harvest food. In addition, exploration of the reserve has identified four temporary ponds located near the water meadows used by hippos during the rainy season. These areas were surrounded by agricultural fields that were often raided by these mammals for food. It is urgent to zone agricultural activity in the area and to grant a special conservation status to the four additional ponds to minimize conflicts between humans and Hippopotamus. Finally, local participation in conservation activities is probably greatly responsible for an increase in the size of the hippo population
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Structure démographique et mouvements saisonniers des populations d’hippopotame commun, Hippopotamus amphibius Linné 1758 dans la Réserve de Biosphère de la Mare aux Hippopotames en zone sud soudanienne du Burkina Faso
2010Co-Authors: Dibloni, Ollo Théophile, Vermeulen Cédric, Guenda Wendengoudi, Millogo Nicolas AlfredAbstract:L’étude de l’effectif, de la structure en classes d’âges et des mouvements de Hippopotamus amphibius a été conduite dans la Réserve de Biosphère de la Mare aux Hippopotames au Burkina Faso. Pendant 3 années d’affilée (2006, 2007 et 2008), des prospections et des inventaires de terrain ont été menés à l’intérieur et à la lisière de la réserve. Les résultats des inventaires ont permis de dénombrer 41 hippopotames en 2008 contre 35 têtes en 2006, répartis en 3 troupeaux distincts. La structure en classes d’âges de cette population était de 32 adultes, 5 subadultes et 4 juvéniles. L’emplacement de leurs aires de repos dans la mare variait suivant le niveau de l’eau. L’inventaire a identifié 8 sites de sorties, sur chaque rive de la mare, utilisées par les hippopotames pour se rendre dans les gagnages. De même, la prospection de la réserve a identifié 4 mares temporaires situées à proximité des prairies aquatiques qui constituaient des zones de migration où les hippopotames se réfugiaient pendant la saison pluvieuse. Ces zones étaient entourées par des champs qui étaient souvent saccagés par ces mammifères pour leur alimentation. Il est urgent de négocier un zonage de l’activité agricole et de conférer un statut particulier de conservation à ces 4 sites afin de minimiser les conflits homme-hippopotame. Les récentes actions de surveillance menées en partenariat avec les communautés locales ont probablement permis l’accroissement de l’effectif de 6 hippopotames en 3 ans.The study was conducted in the “Mare aux Hippopotames” Biosphere Reserve of Burkina Faso and aimed at assessing the number, age structures and seasonal mobility of Hippopotamus amphibius. Methodology used was based on three consecutive-year (2006-2008) monitoring and ground inventory inside and outside the reserve. The results of the inventories have led to the identification of 41 Hippopotamus in 2008 versus 35 in 2006 which are divided into 3 distinct herds. The structure in age group of that population was composed of 32 were adults, 5 sub-adults and 4 juveniles. Location of places where they take a rest varies with the level of the water in the pond. The inventory has identified eight sites on each side of the pond as used by hippos to harvest food. In addition, exploration of the reserve has identified four temporary ponds located near the water meadows used by hippos during the rainy season. These areas were surrounded by agricultural fields that were often raided by these mammals for food. It is urgent to zone agricultural activity in the area and to grant a special conservation status to the four additional ponds to minimize conflicts between humans and Hippopotamus. Finally, local participation in conservation activities is probably greatly responsible for an increase in the size of the hippo population.Peer reviewe
Sharon Joseph - One of the best experts on this subject based on the ideXlab platform.
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endocrine patterns associated with reproduction in the nile Hippopotamus Hippopotamus amphibius as assessed by fecal progestagen analysis
General and Comparative Endocrinology, 2002Co-Authors: Laura H Graham, Kelly Reid, Tricia Webster, Mary Richards, Sharon JosephAbstract:Abstract The Hippopotamus ( Hippopotamus amphibius ) is a popular zoo animal and an integral part of wetland ecosystems in its native continent of Africa. However, information about the reproductive physiology of the Hippopotamus is limited compared to the other megaherbivores of Africa such as the elephant and the rhinoceros. This study is the first to report on the endocrine patterns associated with ovulation and pregnancy in the Nile Hippopotamus. Fecal samples were collected 3 times per week from female hippopotami ( N =9) housed in captivity in Florida. Progesterone metabolites were extracted from the feces with 80% MeOH and quantified using a progestagen EIA. Fecal progestagen analysis indicated hippopotami had a cycle length of 35.3±1.3 days and ovulated throughout the year. Fecal progestagen concentrations during pregnancy ( N =11 pregnancies) were higher on average (833.5±115.2 ng/g; P
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Endocrine patterns associated with reproduction in the Nile Hippopotamus (Hippopotamus amphibius) as assessed by fecal progestagen analysis.
General and comparative endocrinology, 2002Co-Authors: Laura H Graham, Kelly Reid, Tricia Webster, Mary Richards, Sharon JosephAbstract:The Hippopotamus (Hippopotamus amphibius) is a popular zoo animal and an integral part of wetland ecosystems in its native continent of Africa. However, information about the reproductive physiology of the Hippopotamus is limited compared to the other megaherbivores of Africa such as the elephant and the rhinoceros. This study is the first to report on the endocrine patterns associated with ovulation and pregnancy in the Nile Hippopotamus. Fecal samples were collected 3 times per week from female hippopotami (N = 9) housed in captivity in Florida. Progesterone metabolites were extracted from the feces with 80% MeOH and quantified using a progestagen EIA. Fecal progestagen analysis indicated hippopotami had a cycle length of 35.3 +/- 1.3 days and ovulated throughout the year. Fecal progestagen concentrations during pregnancy (N = 11 pregnancies) were higher on average (833.5 +/- 115.2 ng/g; P < 0.05) than those observed during non-pregnant luteal phases (309.5 +/- 20.9 ng/g) and remained elevated throughout gestation. Fecal progestagen analysis revealed the onset of puberty in three females between 3 and 4 years of age and all conceived by their fourth cycle. Lactational suppression of ovulation during nursing was usually, but not always, observed and lasted for 33.9 +/- 1.8 weeks. The observed calving interval in females with lactational anovulation was 17 months. The majority of births (63.6%) occurring during the summer rainy season and the inconsistency of lactational anovulation suggested the possibility of environmental influences on reproduction.
Bo Wang - One of the best experts on this subject based on the ideXlab platform.
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Establishment and characterization of fibroblast cultures derived from a female common Hippopotamus (Hippopotamus amphibius) skin biopsy.
Cell biology international, 2021Co-Authors: Tao Wang, Jinpu Wei, Dongmin Zheng, Chen Wang, Wu Chen, Bo WangAbstract:The population decline of the common Hippopotamus (Hippopotamus amphibius) has necessitated the preservation of their genetic resources for species conservation and research. Of all actions, cryopreservation of fibroblast cell cultures derived from animal biopsy is considered a simple but efficient means. Nevertheless, preserving viable cell cultures of the common Hippopotamus has not been achieved to our knowledge. To this end, we established and characterized fibroblast cell cultures from the skin sample of a newborn common Hippopotamus in this study. By combining the tissue explant direct culture and enzymatic digestion methods, we isolated a great number of cells with typical fibroblastic morphology and high viability. Neither bacteria/fungi nor mycoplasma was detectable in the cell cultures throughout the study. The population doubling time was 34 h according to the growth curve. Karyotyping based on Giemsa staining showed that the cultured cells were diploid with 36 chromosomes in all, one pair of which was sex chromosomes. Amplified mitochondrial cytochrome C oxidase subunit I gene sequence of the cultured cells was 99.26% identical with that of the registered Hippopotamus amphibius complete mitochondrial DNA, confirming the species of origin of the cells. Flow cytometry and immunofluorescence staining results revealed that the detected cells were positive for fibroblast markers, S100A4 and Vimentin. In conclusion, we generated the fibroblast cell cultures from a common Hippopotamus and identified their characteristics using multiple techniques. We believe the cryopreserved cells could be useful genetic materials for future research. This article is protected by copyright. All rights reserved.
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Establishment and characterization of fibroblast cultures derived from a female common Hippopotamus (Hippopotamus amphibius) skin biopsy
2020Co-Authors: Tao Wang, Jinpu Wei, Dongmin Zheng, Chen Wang, Wu Chen, Bo WangAbstract:Abstract The population decline in the common Hippopotamus (Hippopotamus amphibius) has necessitated the preservation of their genetic resources for species conservation and research. Of all actions, cryopreservation of fibroblast cell cultures derived from animal biopsy is considered a simple but efficient means. Nevertheless, preserving viable cell cultures of the common Hippopotamus has not been achieved to our knowledge. To this end, we detailed a method to establish fibroblast cell cultures from a female common Hippopotamus fetus in this study. By combining the classic tissue explant direct culture and enzymatic digestion methods, we isolated a great number of cells with typical fibroblastic morphology and high viability. Characterization of the fibroblast cultures was carried out using different techniques. In short, neither bacteria/fungi nor mycoplasma was detectable in the cell cultures throughout the study. The population doubling time was 23.9 h according to the growth curve. Karyotyping based on Giemsa staining showed that cultured cells were diploid with 36 chromosomes in all, one pair of which was sex chromosomes. Mitochondrial cytochrome C oxidase subunit I gene sequence of the cultured cells was 99.26% identical with the Hippopotamus amphibius complete mitochondrial DNA sequence registered in GenBank, confirming the cells were derived from a common Hippopotamus. Flow cytometry and immunofluorescence staining results revealed that the detected cells were positive for fibroblast markers, S100A4 and Vimentin. In conclusion, we isolated and characterized a new fibroblast cell culture from a common Hippopotamus skin sample and the cryopreserved cells could be useful genetic materials for the future research.
Laura H Graham - One of the best experts on this subject based on the ideXlab platform.
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endocrine patterns associated with reproduction in the nile Hippopotamus Hippopotamus amphibius as assessed by fecal progestagen analysis
General and Comparative Endocrinology, 2002Co-Authors: Laura H Graham, Kelly Reid, Tricia Webster, Mary Richards, Sharon JosephAbstract:Abstract The Hippopotamus ( Hippopotamus amphibius ) is a popular zoo animal and an integral part of wetland ecosystems in its native continent of Africa. However, information about the reproductive physiology of the Hippopotamus is limited compared to the other megaherbivores of Africa such as the elephant and the rhinoceros. This study is the first to report on the endocrine patterns associated with ovulation and pregnancy in the Nile Hippopotamus. Fecal samples were collected 3 times per week from female hippopotami ( N =9) housed in captivity in Florida. Progesterone metabolites were extracted from the feces with 80% MeOH and quantified using a progestagen EIA. Fecal progestagen analysis indicated hippopotami had a cycle length of 35.3±1.3 days and ovulated throughout the year. Fecal progestagen concentrations during pregnancy ( N =11 pregnancies) were higher on average (833.5±115.2 ng/g; P
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Endocrine patterns associated with reproduction in the Nile Hippopotamus (Hippopotamus amphibius) as assessed by fecal progestagen analysis.
General and comparative endocrinology, 2002Co-Authors: Laura H Graham, Kelly Reid, Tricia Webster, Mary Richards, Sharon JosephAbstract:The Hippopotamus (Hippopotamus amphibius) is a popular zoo animal and an integral part of wetland ecosystems in its native continent of Africa. However, information about the reproductive physiology of the Hippopotamus is limited compared to the other megaherbivores of Africa such as the elephant and the rhinoceros. This study is the first to report on the endocrine patterns associated with ovulation and pregnancy in the Nile Hippopotamus. Fecal samples were collected 3 times per week from female hippopotami (N = 9) housed in captivity in Florida. Progesterone metabolites were extracted from the feces with 80% MeOH and quantified using a progestagen EIA. Fecal progestagen analysis indicated hippopotami had a cycle length of 35.3 +/- 1.3 days and ovulated throughout the year. Fecal progestagen concentrations during pregnancy (N = 11 pregnancies) were higher on average (833.5 +/- 115.2 ng/g; P < 0.05) than those observed during non-pregnant luteal phases (309.5 +/- 20.9 ng/g) and remained elevated throughout gestation. Fecal progestagen analysis revealed the onset of puberty in three females between 3 and 4 years of age and all conceived by their fourth cycle. Lactational suppression of ovulation during nursing was usually, but not always, observed and lasted for 33.9 +/- 1.8 weeks. The observed calving interval in females with lactational anovulation was 17 months. The majority of births (63.6%) occurring during the summer rainy season and the inconsistency of lactational anovulation suggested the possibility of environmental influences on reproduction.
Tao Wang - One of the best experts on this subject based on the ideXlab platform.
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Establishment and characterization of fibroblast cultures derived from a female common Hippopotamus (Hippopotamus amphibius) skin biopsy.
Cell biology international, 2021Co-Authors: Tao Wang, Jinpu Wei, Dongmin Zheng, Chen Wang, Wu Chen, Bo WangAbstract:The population decline of the common Hippopotamus (Hippopotamus amphibius) has necessitated the preservation of their genetic resources for species conservation and research. Of all actions, cryopreservation of fibroblast cell cultures derived from animal biopsy is considered a simple but efficient means. Nevertheless, preserving viable cell cultures of the common Hippopotamus has not been achieved to our knowledge. To this end, we established and characterized fibroblast cell cultures from the skin sample of a newborn common Hippopotamus in this study. By combining the tissue explant direct culture and enzymatic digestion methods, we isolated a great number of cells with typical fibroblastic morphology and high viability. Neither bacteria/fungi nor mycoplasma was detectable in the cell cultures throughout the study. The population doubling time was 34 h according to the growth curve. Karyotyping based on Giemsa staining showed that the cultured cells were diploid with 36 chromosomes in all, one pair of which was sex chromosomes. Amplified mitochondrial cytochrome C oxidase subunit I gene sequence of the cultured cells was 99.26% identical with that of the registered Hippopotamus amphibius complete mitochondrial DNA, confirming the species of origin of the cells. Flow cytometry and immunofluorescence staining results revealed that the detected cells were positive for fibroblast markers, S100A4 and Vimentin. In conclusion, we generated the fibroblast cell cultures from a common Hippopotamus and identified their characteristics using multiple techniques. We believe the cryopreserved cells could be useful genetic materials for future research. This article is protected by copyright. All rights reserved.
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Establishment and characterization of fibroblast cultures derived from a female common Hippopotamus (Hippopotamus amphibius) skin biopsy
2020Co-Authors: Tao Wang, Jinpu Wei, Dongmin Zheng, Chen Wang, Wu Chen, Bo WangAbstract:Abstract The population decline in the common Hippopotamus (Hippopotamus amphibius) has necessitated the preservation of their genetic resources for species conservation and research. Of all actions, cryopreservation of fibroblast cell cultures derived from animal biopsy is considered a simple but efficient means. Nevertheless, preserving viable cell cultures of the common Hippopotamus has not been achieved to our knowledge. To this end, we detailed a method to establish fibroblast cell cultures from a female common Hippopotamus fetus in this study. By combining the classic tissue explant direct culture and enzymatic digestion methods, we isolated a great number of cells with typical fibroblastic morphology and high viability. Characterization of the fibroblast cultures was carried out using different techniques. In short, neither bacteria/fungi nor mycoplasma was detectable in the cell cultures throughout the study. The population doubling time was 23.9 h according to the growth curve. Karyotyping based on Giemsa staining showed that cultured cells were diploid with 36 chromosomes in all, one pair of which was sex chromosomes. Mitochondrial cytochrome C oxidase subunit I gene sequence of the cultured cells was 99.26% identical with the Hippopotamus amphibius complete mitochondrial DNA sequence registered in GenBank, confirming the cells were derived from a common Hippopotamus. Flow cytometry and immunofluorescence staining results revealed that the detected cells were positive for fibroblast markers, S100A4 and Vimentin. In conclusion, we isolated and characterized a new fibroblast cell culture from a common Hippopotamus skin sample and the cryopreserved cells could be useful genetic materials for the future research.