The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
Christiane Bormann - One of the best experts on this subject based on the ideXlab platform.
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Histidine aminotransferase activity in Streptomyces tendae and its correlation with nikkomycin production
Fems Microbiology Letters, 1992Co-Authors: Ulrich Roos, Sibylle Mattern, Hildgund Schrempf, Christiane BormannAbstract:Streptomyces tendae Tu901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in Histidine catabolism were isolated and characterized with regard to their Histidine ammonium-lyase activity and antibiotic synthesis. In the Histidine Ammonialyase-negative mutant hut-11 which was unimpaired in nikkomycin production Histidine amino-transferase activity was detected as an additional Histidine metabolizing enzyme. A protein exhibiting Histidine aminotransferase activity could be demonstrated on non-denaturing gels of hut-11 crude extracts. Using optimized assay conditions, Histidine aminotransferase activity was investigated in the strain hut-11 during growth in nikkomycin production medium. Maximal activity was reached at the end of exponential growth prior to nikkomycin production. In the presence of bromopyruvate, an effective inhibitor of Histidine aminotransferase activity in vitro, production of nikkomycin Z and X was markedly reduced in hut-11.
Ulrich Roos - One of the best experts on this subject based on the ideXlab platform.
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Histidine aminotransferase activity in Streptomyces tendae and its correlation with nikkomycin production
Fems Microbiology Letters, 1992Co-Authors: Ulrich Roos, Sibylle Mattern, Hildgund Schrempf, Christiane BormannAbstract:Streptomyces tendae Tu901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in Histidine catabolism were isolated and characterized with regard to their Histidine ammonium-lyase activity and antibiotic synthesis. In the Histidine Ammonialyase-negative mutant hut-11 which was unimpaired in nikkomycin production Histidine amino-transferase activity was detected as an additional Histidine metabolizing enzyme. A protein exhibiting Histidine aminotransferase activity could be demonstrated on non-denaturing gels of hut-11 crude extracts. Using optimized assay conditions, Histidine aminotransferase activity was investigated in the strain hut-11 during growth in nikkomycin production medium. Maximal activity was reached at the end of exponential growth prior to nikkomycin production. In the presence of bromopyruvate, an effective inhibitor of Histidine aminotransferase activity in vitro, production of nikkomycin Z and X was markedly reduced in hut-11.
János Rétey - One of the best experts on this subject based on the ideXlab platform.
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Formation of the Michaelis complex without involvement of the prosthetic group dehydroalanine of Histidine Ammonialyase
Bioorganic & Medicinal Chemistry Letters, 1997Co-Authors: Birgid Langer, Julia Starck, Martin Langer, János RéteyAbstract:Abstract The dehydroalanine-less S143G mutant of Histidine ammonia-lyase was constructed and used for kinetic measurements with 5′-nitro-Histidine as a substrate. The natural substrate Histidine turned out to be a competitive inhibitor of the mutant enzyme and exhibited a K i value which was similar to its K m value with the wild-type enzyme. Thus the dehydroalanine prosthetic group does not play a role in formation of the Michaelis complex.
Sibylle Mattern - One of the best experts on this subject based on the ideXlab platform.
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Histidine aminotransferase activity in Streptomyces tendae and its correlation with nikkomycin production
Fems Microbiology Letters, 1992Co-Authors: Ulrich Roos, Sibylle Mattern, Hildgund Schrempf, Christiane BormannAbstract:Streptomyces tendae Tu901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in Histidine catabolism were isolated and characterized with regard to their Histidine ammonium-lyase activity and antibiotic synthesis. In the Histidine Ammonialyase-negative mutant hut-11 which was unimpaired in nikkomycin production Histidine amino-transferase activity was detected as an additional Histidine metabolizing enzyme. A protein exhibiting Histidine aminotransferase activity could be demonstrated on non-denaturing gels of hut-11 crude extracts. Using optimized assay conditions, Histidine aminotransferase activity was investigated in the strain hut-11 during growth in nikkomycin production medium. Maximal activity was reached at the end of exponential growth prior to nikkomycin production. In the presence of bromopyruvate, an effective inhibitor of Histidine aminotransferase activity in vitro, production of nikkomycin Z and X was markedly reduced in hut-11.
Hildgund Schrempf - One of the best experts on this subject based on the ideXlab platform.
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Histidine aminotransferase activity in Streptomyces tendae and its correlation with nikkomycin production
Fems Microbiology Letters, 1992Co-Authors: Ulrich Roos, Sibylle Mattern, Hildgund Schrempf, Christiane BormannAbstract:Streptomyces tendae Tu901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in Histidine catabolism were isolated and characterized with regard to their Histidine ammonium-lyase activity and antibiotic synthesis. In the Histidine Ammonialyase-negative mutant hut-11 which was unimpaired in nikkomycin production Histidine amino-transferase activity was detected as an additional Histidine metabolizing enzyme. A protein exhibiting Histidine aminotransferase activity could be demonstrated on non-denaturing gels of hut-11 crude extracts. Using optimized assay conditions, Histidine aminotransferase activity was investigated in the strain hut-11 during growth in nikkomycin production medium. Maximal activity was reached at the end of exponential growth prior to nikkomycin production. In the presence of bromopyruvate, an effective inhibitor of Histidine aminotransferase activity in vitro, production of nikkomycin Z and X was markedly reduced in hut-11.