The Experts below are selected from a list of 10062 Experts worldwide ranked by ideXlab platform
Joshua D. Nosanchuk - One of the best experts on this subject based on the ideXlab platform.
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Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus.
Cellular microbiology, 2020Co-Authors: Levi G. Cleare, Daniel Zamith, Heino M. Heyman, Sneha P. Couvillion, Leonardo Nimrichter, Marcio L. Rodrigues, Ernesto S. Nakayasu, Joshua D. NosanchukAbstract:Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. Histoplasma capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. Histoplasma capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In this study, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.
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Histoplasma capsulatum: Mechanisms for Pathogenesis.
Current Topics in Microbiology and Immunology, 2018Co-Authors: Jamie Mittal, Maria G Ponce, Inessa Gendlina, Joshua D. NosanchukAbstract:Histoplasmosis, caused by the dimorphic environmental fungus Histoplasma capsulatum, is a major mycosis on the global stage. Acquisition of the fungus by mammalian hosts can be clinically silent or it can lead to life-threatening systemic disease, which can occur in immunologically intact or deficient hosts, albeit severe disease is more likely in the setting of compromised cellular immunity. H. capsulatum yeast cells are highly adapted to the mammalian host as they can effectively survive within intracellular niches in select phagocytic cells. Understanding the biological response by both the host and H. capsulatum will facilitate improved approaches to prevent and/or modify disease. This review presents our current understanding of the major pathogenic mechanisms involved in histoplasmosis.
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Surface architecture of Histoplasma capsulatum
Frontiers in Microbiology, 2011Co-Authors: Allan J. Guimarães, Mariana Duarte De Cerqueira, Joshua D. NosanchukAbstract:The dimorphic fungal pathogen Histoplasma capsulatum is the most frequent cause of clinically significant fungal pneumonia in humans. H. capsulatum virulence is achieved, in part, through diverse and dynamic alterations to the fungal cell surface. Surface components associated with H. capsulatum pathogenicity include carbohydrates, lipids, proteins, and melanins. Here, we describe the various structures comprising the cell surface of H. capsulatum that have been associated with virulence and discuss their involvement on the pathobiology of disease.
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Histoplasma capsulatum at the host–pathogen interface
Microbes and Infection, 2008Co-Authors: Joshua D. Nosanchuk, Attila GácserAbstract:Histoplasma capsulatum is the most common cause of invasive fungal pulmonary disease worldwide. The interaction of H. capsulatum with a host is a complex, dynamic process. Severe disease most commonly occurs in individuals with compromised immunity, and the increasing utilization of immunomodulators in medicine has revealed significant risks for reactivation disease in patients with latent histoplasmosis. Fortunately, there are well developed molecular tools and excellent animal models for studying H. capsulatum virulence and numerous recent advances have been made regarding the pathogenesis of this fungus that will improve our capacity to combat disease.
Gary W. Procop - One of the best experts on this subject based on the ideXlab platform.
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Identification of Histoplasma capsulatum from Culture Extracts by Real-Time PCR
Journal of Clinical Microbiology, 2003Co-Authors: Jose Martagon-villamil, Nabin K. Shrestha, Mary Sholtis, Carlos M. Isada, Gerri S. Hall, Terry Bryne, Barbara Alexander Lodge, L. Barth Reller, Gary W. ProcopAbstract:We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
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Rapid Diagnosis of Histoplasma capsulatum Endocarditis Using the AccuProbe on an Excised Valve
Journal of Clinical Microbiology, 2001Co-Authors: Roy F. Chemaly, Mary Sholtis, Gerri S. Hall, J. W. Tomford, Jimmy Chua, Gary W. ProcopAbstract:Histoplasma capsulatum is an infrequent but serious cause of endocarditis. The definitive diagnosis requires culture, which may require a long incubation. We demonstrated the ability of the Histoplasma capsulatum AccuProbe to accurately identify this organism when applied directly on an excised valve that contained abundant yeast forms consistent with H. capsulatum.
Michael R. Jacobs - One of the best experts on this subject based on the ideXlab platform.
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Histoplasma capsulatum Prosthetic Valve Endocarditis with Negative Fungal Blood Cultures and Negative Histoplasma Antigen Assay in an Immunocompetent Patient
Journal of clinical microbiology, 2010Co-Authors: Sadao Jinno, Barbara M. Gripshover, Tracy L. Lemonovich, James M. Anderson, Michael R. JacobsAbstract:We report a case of Histoplasma capsulatum endocarditis in which Histoplasma antigen assay and fungal blood cultures were negative. The diagnosis was made by microscopic examination and culture of the excised valve. Histoplasma capsulatum should be considered in the differential diagnosis of culture-negative endocarditis in regions where it is endemic and in travelers.
Sadao Jinno - One of the best experts on this subject based on the ideXlab platform.
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Histoplasma capsulatum Prosthetic Valve Endocarditis with Negative Fungal Blood Cultures and Negative Histoplasma Antigen Assay in an Immunocompetent Patient
Journal of clinical microbiology, 2010Co-Authors: Sadao Jinno, Barbara M. Gripshover, Tracy L. Lemonovich, James M. Anderson, Michael R. JacobsAbstract:We report a case of Histoplasma capsulatum endocarditis in which Histoplasma antigen assay and fungal blood cultures were negative. The diagnosis was made by microscopic examination and culture of the excised valve. Histoplasma capsulatum should be considered in the differential diagnosis of culture-negative endocarditis in regions where it is endemic and in travelers.
Gerri S. Hall - One of the best experts on this subject based on the ideXlab platform.
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Identification of Histoplasma capsulatum from Culture Extracts by Real-Time PCR
Journal of Clinical Microbiology, 2003Co-Authors: Jose Martagon-villamil, Nabin K. Shrestha, Mary Sholtis, Carlos M. Isada, Gerri S. Hall, Terry Bryne, Barbara Alexander Lodge, L. Barth Reller, Gary W. ProcopAbstract:We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
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Rapid Diagnosis of Histoplasma capsulatum Endocarditis Using the AccuProbe on an Excised Valve
Journal of Clinical Microbiology, 2001Co-Authors: Roy F. Chemaly, Mary Sholtis, Gerri S. Hall, J. W. Tomford, Jimmy Chua, Gary W. ProcopAbstract:Histoplasma capsulatum is an infrequent but serious cause of endocarditis. The definitive diagnosis requires culture, which may require a long incubation. We demonstrated the ability of the Histoplasma capsulatum AccuProbe to accurately identify this organism when applied directly on an excised valve that contained abundant yeast forms consistent with H. capsulatum.