The Experts below are selected from a list of 27 Experts worldwide ranked by ideXlab platform

Takeo Juji - One of the best experts on this subject based on the ideXlab platform.

  • HLA A null Allele with A stop codon HLA A 0215n identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele (A*0215N) wAs the sAme As thAt of A*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A*0207.

  • HLA-A null Allele with A stop codon, HLA-A ^* 0215N, identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A ^*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele ( A ^*0215N) wAs the sAme As thAt of A ^*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A ^*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A ^*0207.

Yoshihide Ishikawa - One of the best experts on this subject based on the ideXlab platform.

  • HLA A null Allele with A stop codon HLA A 0215n identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele (A*0215N) wAs the sAme As thAt of A*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A*0207.

  • HLA-A null Allele with A stop codon, HLA-A ^* 0215N, identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A ^*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele ( A ^*0215N) wAs the sAme As thAt of A ^*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A ^*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A ^*0207.

Hidenori Tanaka - One of the best experts on this subject based on the ideXlab platform.

  • HLA A null Allele with A stop codon HLA A 0215n identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele (A*0215N) wAs the sAme As thAt of A*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A*0207.

  • HLA-A null Allele with A stop codon, HLA-A ^* 0215N, identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A ^*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele ( A ^*0215N) wAs the sAme As thAt of A ^*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A ^*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A ^*0207.

Motoko Nishimura - One of the best experts on this subject based on the ideXlab platform.

  • HLA A null Allele with A stop codon HLA A 0215n identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele (A*0215N) wAs the sAme As thAt of A*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A*0207.

  • HLA-A null Allele with A stop codon, HLA-A ^* 0215N, identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A ^*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele ( A ^*0215N) wAs the sAme As thAt of A ^*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A ^*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A ^*0207.

Masato Muraoka - One of the best experts on this subject based on the ideXlab platform.

  • HLA A null Allele with A stop codon HLA A 0215n identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele (A*0215N) wAs the sAme As thAt of A*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A*0207.

  • HLA-A null Allele with A stop codon, HLA-A ^* 0215N, identified in A homozygous stAte in A heAlthy Adult
    Immunogenetics, 1995
    Co-Authors: Yoshihide Ishikawa, Katsushi Tokunaga, Hidenori Tanaka, Motoko Nishimura, Masato Muraoka, Yasuhiko Fujii, Tatsuya Akaza, Kenji Tadokoro, Takeo Juji
    Abstract:

    A heAlthy Adult hAving no serologicAlly detectAble HLA clAss I A locus Antigens wAs identified. The pArents of the individuAl Are consAnguineous. Results of A fAmily study indicAted thAt the individuAl is homozygous for the B46-Cw1-DR8.1 hAplotype, which wAs shown to be positively AssociAted with A ^*0207 in our previous study. The HLA-A null individuAl is heAlthy And exhibits no AppArent immunologicAl AbnormAlity. TotAl RNAs extrActed from peripherAl blood were converted to cDNAs. The reverse trAnscriptAse-polymerAse chAin reAction (PCR) product, which is of the sAme size As the normAlly expressed gene, wAs eAsily obtAined from the cDNAs with HLA-A locus-specific primers. The nucleotide sequence of this null Allele ( A ^*0215N) wAs the sAme As thAt of A ^*0207 except for A single nucleotide substitution which resulted in A stop codon in exon 4. From its nucleotide sequence, A truncAted molecule wAs expected to be produced; however, the immunoprecipitAtion study fAiled to detect the predicted product. Genomic DNAs from 29 unrelAted individuAls who expressed only one HLA-A Antigen with HLA-B46, were AnAlyzed by A PCR-sequence-specific oligonucleotide method. None of the sAmples possessed this stop codon. Therefore, A ^*0215N is likely to be A rAre Allele generAted by A single point mutAtion from A ^*0207.