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S Feki - One of the best experts on this subject based on the ideXlab platform.

  • ab0016 association between the HLA B51 Antigen and profiles of peripheral t cell subsets in tunisian patients with behcet s disease
    Annals of the Rheumatic Diseases, 2013
    Co-Authors: K Sakly, C Lambert, M Maatouk, S Hammami, N Sakly, O Harzallah, S Mahjoub, Ibtissem Ghedira, S Feki
    Abstract:

    Background Behcet’s disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1]. An association between Behcet’s disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2]. Objectives The aim of this study was to assess possible associations between the expression of HLA-B51 Antigen and particular profiles of peripheral T cell subsets in patients with BD. Methods 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique. Results Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naive T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naive T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells. Conclusions Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 Antigen expression in the pathogenesis of BD. References Kaneko, F., et al., Behcet’s disease (Adamantiades-Behcet’s disease). Clin Dev Immunol, 2011. 2011: p. 681956. Disclosure of Interest None Declared

K Sakly - One of the best experts on this subject based on the ideXlab platform.

  • ab0016 association between the HLA B51 Antigen and profiles of peripheral t cell subsets in tunisian patients with behcet s disease
    Annals of the Rheumatic Diseases, 2013
    Co-Authors: K Sakly, C Lambert, M Maatouk, S Hammami, N Sakly, O Harzallah, S Mahjoub, Ibtissem Ghedira, S Feki
    Abstract:

    Background Behcet’s disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1]. An association between Behcet’s disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2]. Objectives The aim of this study was to assess possible associations between the expression of HLA-B51 Antigen and particular profiles of peripheral T cell subsets in patients with BD. Methods 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique. Results Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naive T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naive T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells. Conclusions Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 Antigen expression in the pathogenesis of BD. References Kaneko, F., et al., Behcet’s disease (Adamantiades-Behcet’s disease). Clin Dev Immunol, 2011. 2011: p. 681956. Disclosure of Interest None Declared

O Harzallah - One of the best experts on this subject based on the ideXlab platform.

  • ab0016 association between the HLA B51 Antigen and profiles of peripheral t cell subsets in tunisian patients with behcet s disease
    Annals of the Rheumatic Diseases, 2013
    Co-Authors: K Sakly, C Lambert, M Maatouk, S Hammami, N Sakly, O Harzallah, S Mahjoub, Ibtissem Ghedira, S Feki
    Abstract:

    Background Behcet’s disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1]. An association between Behcet’s disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2]. Objectives The aim of this study was to assess possible associations between the expression of HLA-B51 Antigen and particular profiles of peripheral T cell subsets in patients with BD. Methods 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique. Results Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naive T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naive T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells. Conclusions Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 Antigen expression in the pathogenesis of BD. References Kaneko, F., et al., Behcet’s disease (Adamantiades-Behcet’s disease). Clin Dev Immunol, 2011. 2011: p. 681956. Disclosure of Interest None Declared

Ibtissem Ghedira - One of the best experts on this subject based on the ideXlab platform.

  • ab0016 association between the HLA B51 Antigen and profiles of peripheral t cell subsets in tunisian patients with behcet s disease
    Annals of the Rheumatic Diseases, 2013
    Co-Authors: K Sakly, C Lambert, M Maatouk, S Hammami, N Sakly, O Harzallah, S Mahjoub, Ibtissem Ghedira, S Feki
    Abstract:

    Background Behcet’s disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1]. An association between Behcet’s disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2]. Objectives The aim of this study was to assess possible associations between the expression of HLA-B51 Antigen and particular profiles of peripheral T cell subsets in patients with BD. Methods 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique. Results Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naive T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naive T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells. Conclusions Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 Antigen expression in the pathogenesis of BD. References Kaneko, F., et al., Behcet’s disease (Adamantiades-Behcet’s disease). Clin Dev Immunol, 2011. 2011: p. 681956. Disclosure of Interest None Declared

S Mahjoub - One of the best experts on this subject based on the ideXlab platform.

  • ab0016 association between the HLA B51 Antigen and profiles of peripheral t cell subsets in tunisian patients with behcet s disease
    Annals of the Rheumatic Diseases, 2013
    Co-Authors: K Sakly, C Lambert, M Maatouk, S Hammami, N Sakly, O Harzallah, S Mahjoub, Ibtissem Ghedira, S Feki
    Abstract:

    Background Behcet’s disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1]. An association between Behcet’s disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2]. Objectives The aim of this study was to assess possible associations between the expression of HLA-B51 Antigen and particular profiles of peripheral T cell subsets in patients with BD. Methods 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique. Results Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naive T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naive T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells. Conclusions Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 Antigen expression in the pathogenesis of BD. References Kaneko, F., et al., Behcet’s disease (Adamantiades-Behcet’s disease). Clin Dev Immunol, 2011. 2011: p. 681956. Disclosure of Interest None Declared