The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform

Ali Bagherian - One of the best experts on this subject based on the ideXlab platform.

  • ASSOCIATION BETWEEN HLADRB1 04* AND HLAADQB106* WITH SEVERE EARLY CHILDHOOD CARIES
    2007
    Co-Authors: Hossein Nematollahi, J Tavakol Afshari, Ali Bagherian
    Abstract:

    Introduction: Severe Early Childhood Caries (SECC) is one of the most common diseases in childhood. Etiology of SECC is multifactorial and both genetic and environmental factors play important roles in the pathogenesis of the disease. Genetic variation of the host may contribute to susceptibility for dental caries. Genetic factors such as Human Leukocyte Antigen (HLA) have been recently introduced as a predisposing factor. The aim of this study was to look for an association between HLA-DRB1*04 and HLADQB1*06 with SECC for early diagnosis as well as prevention of the disease. Materials & Methods: In this cross-sectional study we extracted the genomic DNAS from the whole blood samples of 44 patients with SECC and 35 caries free children (control group) by salting out method. We amplified the genomic DNA by PCR sequence specific primer (PCR-SSP) and then HLA-typing was performed for both alleles. The data were analyzed using Logistic Regression, Fisher's exact, chi-square and Student t test with 95% significance level. Results: The results revealed a significant increase in the frequency of HLADRB1*04 in the patient group (P-value=0.019). The odds ratio for this allele was detected to be 10. Frequency of HLA-DQB1*06 allele was not significantly different between the two groups (P-value=0.37). Conclusion: The above results suggest that HLA-DRB1*04 maybe related to the susceptibility to SECC. Thus HLADRB1*04 detection as a molecular marker for early diagnosis of SECC can be recommended. Key words: HLA-DRB1 Antigen, HLA-DQB1 Antigen, early childhood caries.

Hossein Nematollahi - One of the best experts on this subject based on the ideXlab platform.

  • ASSOCIATION BETWEEN HLADRB1 04* AND HLAADQB106* WITH SEVERE EARLY CHILDHOOD CARIES
    2007
    Co-Authors: Hossein Nematollahi, J Tavakol Afshari, Ali Bagherian
    Abstract:

    Introduction: Severe Early Childhood Caries (SECC) is one of the most common diseases in childhood. Etiology of SECC is multifactorial and both genetic and environmental factors play important roles in the pathogenesis of the disease. Genetic variation of the host may contribute to susceptibility for dental caries. Genetic factors such as Human Leukocyte Antigen (HLA) have been recently introduced as a predisposing factor. The aim of this study was to look for an association between HLA-DRB1*04 and HLADQB1*06 with SECC for early diagnosis as well as prevention of the disease. Materials & Methods: In this cross-sectional study we extracted the genomic DNAS from the whole blood samples of 44 patients with SECC and 35 caries free children (control group) by salting out method. We amplified the genomic DNA by PCR sequence specific primer (PCR-SSP) and then HLA-typing was performed for both alleles. The data were analyzed using Logistic Regression, Fisher's exact, chi-square and Student t test with 95% significance level. Results: The results revealed a significant increase in the frequency of HLADRB1*04 in the patient group (P-value=0.019). The odds ratio for this allele was detected to be 10. Frequency of HLA-DQB1*06 allele was not significantly different between the two groups (P-value=0.37). Conclusion: The above results suggest that HLA-DRB1*04 maybe related to the susceptibility to SECC. Thus HLADRB1*04 detection as a molecular marker for early diagnosis of SECC can be recommended. Key words: HLA-DRB1 Antigen, HLA-DQB1 Antigen, early childhood caries.

J Tavakol Afshari - One of the best experts on this subject based on the ideXlab platform.

  • ASSOCIATION BETWEEN HLADRB1 04* AND HLAADQB106* WITH SEVERE EARLY CHILDHOOD CARIES
    2007
    Co-Authors: Hossein Nematollahi, J Tavakol Afshari, Ali Bagherian
    Abstract:

    Introduction: Severe Early Childhood Caries (SECC) is one of the most common diseases in childhood. Etiology of SECC is multifactorial and both genetic and environmental factors play important roles in the pathogenesis of the disease. Genetic variation of the host may contribute to susceptibility for dental caries. Genetic factors such as Human Leukocyte Antigen (HLA) have been recently introduced as a predisposing factor. The aim of this study was to look for an association between HLA-DRB1*04 and HLADQB1*06 with SECC for early diagnosis as well as prevention of the disease. Materials & Methods: In this cross-sectional study we extracted the genomic DNAS from the whole blood samples of 44 patients with SECC and 35 caries free children (control group) by salting out method. We amplified the genomic DNA by PCR sequence specific primer (PCR-SSP) and then HLA-typing was performed for both alleles. The data were analyzed using Logistic Regression, Fisher's exact, chi-square and Student t test with 95% significance level. Results: The results revealed a significant increase in the frequency of HLADRB1*04 in the patient group (P-value=0.019). The odds ratio for this allele was detected to be 10. Frequency of HLA-DQB1*06 allele was not significantly different between the two groups (P-value=0.37). Conclusion: The above results suggest that HLA-DRB1*04 maybe related to the susceptibility to SECC. Thus HLADRB1*04 detection as a molecular marker for early diagnosis of SECC can be recommended. Key words: HLA-DRB1 Antigen, HLA-DQB1 Antigen, early childhood caries.

Gerald Wulf - One of the best experts on this subject based on the ideXlab platform.

  • High resolution HLA-matching in hematopoietic stem cell transplantation: a retrospective collaborative analysis
    Blood, 2013
    Co-Authors: Daniel Fürst, Carlheinz Müller, Vladan Vucinic, Donald Bunjes, Wolfgang Herr, Martin Gramatzki, Rainer Schwerdtfeger, Renate Arnold, Hermann Einsele, Gerald Wulf
    Abstract:

    To validate current donor selection strategies based on previous international studies, we retrospectively analyzed 2646 transplantations performed for hematologic malignancies in 28 German transplant centers. Donors and recipients were high resolution typed for HLA-A, -B, -C, -DRB1, and -DQB1. The highest mortality in overall survival analysis was seen for HLA-A, -B, and DRB1 mismatches. HLA-DQB1 mismatched cases showed a trend toward higher mortality, mostly due to HLA-DQB1 Antigen disparities. HLA incompatibilities at >1 locus showed additive detrimental effects. HLA mismatching had no significant effect on relapse incidence and primary graft failure. Graft source had no impact on survival end points, neither in univariate nor in multivariate analysis. Higher patient age, advanced disease, transplantations before 2004, patient C2C2 killer cell immunoglobulin-like receptor (KIR)-ligand phenotype, and unavailability of a national donor adversely influenced outcomes in multivariate analysis. Our study confirms the association of HLA-A, -B, -C, and -DRB1 incompatibilities with adverse outcome in hematopoietic stem cell transplantation (HSCT). The relevance of HLA-DQB1 disparities in single mismatched transplantations remains unclear. Similar hazard ratios for allele and Antigen mismatches (possibly with an exception for HLA-DQB1) highlight the importance of allele level typing and matching in HSCT. The number of incompatibilities and their type significantly impact survival.

Daniel Fürst - One of the best experts on this subject based on the ideXlab platform.

  • High resolution HLA-matching in hematopoietic stem cell transplantation: a retrospective collaborative analysis
    Blood, 2013
    Co-Authors: Daniel Fürst, Carlheinz Müller, Vladan Vucinic, Donald Bunjes, Wolfgang Herr, Martin Gramatzki, Rainer Schwerdtfeger, Renate Arnold, Hermann Einsele, Gerald Wulf
    Abstract:

    To validate current donor selection strategies based on previous international studies, we retrospectively analyzed 2646 transplantations performed for hematologic malignancies in 28 German transplant centers. Donors and recipients were high resolution typed for HLA-A, -B, -C, -DRB1, and -DQB1. The highest mortality in overall survival analysis was seen for HLA-A, -B, and DRB1 mismatches. HLA-DQB1 mismatched cases showed a trend toward higher mortality, mostly due to HLA-DQB1 Antigen disparities. HLA incompatibilities at >1 locus showed additive detrimental effects. HLA mismatching had no significant effect on relapse incidence and primary graft failure. Graft source had no impact on survival end points, neither in univariate nor in multivariate analysis. Higher patient age, advanced disease, transplantations before 2004, patient C2C2 killer cell immunoglobulin-like receptor (KIR)-ligand phenotype, and unavailability of a national donor adversely influenced outcomes in multivariate analysis. Our study confirms the association of HLA-A, -B, -C, and -DRB1 incompatibilities with adverse outcome in hematopoietic stem cell transplantation (HSCT). The relevance of HLA-DQB1 disparities in single mismatched transplantations remains unclear. Similar hazard ratios for allele and Antigen mismatches (possibly with an exception for HLA-DQB1) highlight the importance of allele level typing and matching in HSCT. The number of incompatibilities and their type significantly impact survival.