The Experts below are selected from a list of 6093 Experts worldwide ranked by ideXlab platform

Lara Lewis Mcgrath - One of the best experts on this subject based on the ideXlab platform.

  • de novo transcriptome assembly for the lobster Homarus americanus and characterization of differential gene expression across nervous system tissues
    BMC Genomics, 2016
    Co-Authors: Lara Lewis Mcgrath, Steven V Vollmer, Stefan T Kaluziak, Joseph Ayers
    Abstract:

    The American lobster, Homarus americanus, is an important species as an economically valuable fishery, a key member in marine ecosystems, and a well-studied model for central pattern generation, the neural networks that control rhythmic motor patterns. Despite multi-faceted scientific interest in this species, currently our genetic resources for the lobster are limited. In this study, we de novo assemble a transcriptome for Homarus americanus using central nervous system (CNS), muscle, and hybrid neurosecretory tissues and compare gene expression across these tissue types. In particular, we focus our analysis on genes relevant to central pattern generation and the identity of the neurons in a neural network, which is defined by combinations of genes distinguishing the neuronal behavior and phenotype, including ion channels, neurotransmitters, neuromodulators, receptors, transcription factors, and other gene products. Using samples from the central nervous system (brain, abdominal ganglia), abdominal muscle, and heart (cardiac ganglia, pericardial organs, muscle), we used RNA-Seq to characterize gene expression patterns across tissues types. We also compared control tissues with those challenged with the neuropeptide proctolin in vivo. Our transcriptome generated 34,813 transcripts with known protein annotations. Of these, 5,000-10,000 of annotated transcripts were significantly differentially expressed (DE) across tissue types. We found 421 transcripts for ion channels and identified receptors and/or proteins for over 20 different neurotransmitters and neuromodulators. Results indicated tissue-specific expression of select neuromodulator (allostatin, myomodulin, octopamine, nitric oxide) and neurotransmitter (glutamate, acetylcholine) pathways. We also identify differential expression of ion channel families, including kainite family glutamate receptors, inward-rectifying K+ (IRK) channels, and transient receptor potential (TRP) A family channels, across central pattern generating tissues. Our transcriptome-wide profiles of the rhythmic pattern generating abdominal and cardiac nervous systems in Homarus americanus reveal candidates for neuronal features that drive the production of motor output in these systems.

Marcia Bowen - One of the best experts on this subject based on the ideXlab platform.

Joseph Ayers - One of the best experts on this subject based on the ideXlab platform.

  • de novo transcriptome assembly for the lobster Homarus americanus and characterization of differential gene expression across nervous system tissues
    BMC Genomics, 2016
    Co-Authors: Lara Lewis Mcgrath, Steven V Vollmer, Stefan T Kaluziak, Joseph Ayers
    Abstract:

    The American lobster, Homarus americanus, is an important species as an economically valuable fishery, a key member in marine ecosystems, and a well-studied model for central pattern generation, the neural networks that control rhythmic motor patterns. Despite multi-faceted scientific interest in this species, currently our genetic resources for the lobster are limited. In this study, we de novo assemble a transcriptome for Homarus americanus using central nervous system (CNS), muscle, and hybrid neurosecretory tissues and compare gene expression across these tissue types. In particular, we focus our analysis on genes relevant to central pattern generation and the identity of the neurons in a neural network, which is defined by combinations of genes distinguishing the neuronal behavior and phenotype, including ion channels, neurotransmitters, neuromodulators, receptors, transcription factors, and other gene products. Using samples from the central nervous system (brain, abdominal ganglia), abdominal muscle, and heart (cardiac ganglia, pericardial organs, muscle), we used RNA-Seq to characterize gene expression patterns across tissues types. We also compared control tissues with those challenged with the neuropeptide proctolin in vivo. Our transcriptome generated 34,813 transcripts with known protein annotations. Of these, 5,000-10,000 of annotated transcripts were significantly differentially expressed (DE) across tissue types. We found 421 transcripts for ion channels and identified receptors and/or proteins for over 20 different neurotransmitters and neuromodulators. Results indicated tissue-specific expression of select neuromodulator (allostatin, myomodulin, octopamine, nitric oxide) and neurotransmitter (glutamate, acetylcholine) pathways. We also identify differential expression of ion channel families, including kainite family glutamate receptors, inward-rectifying K+ (IRK) channels, and transient receptor potential (TRP) A family channels, across central pattern generating tissues. Our transcriptome-wide profiles of the rhythmic pattern generating abdominal and cardiac nervous systems in Homarus americanus reveal candidates for neuronal features that drive the production of motor output in these systems.

R. W. Elner - One of the best experts on this subject based on the ideXlab platform.

  • Temperature and Lobster, Homarus americanus, Yield Relationships
    Canadian Journal of Fisheries and Aquatic Sciences, 1991
    Co-Authors: A. Campbell, D. J. Noakes, R. W. Elner
    Abstract:

    Commercial landings of American lobster (Homarus americanus) have increased dramatically in many areas along the east coast of Canada since the late 1970's. Average annual sea surface temperatures (SST) were analysed to test if lobster landings were related to changes in the marine environment. Time series models were fitted to lobster landings and SST data from Maine (USA), Halifax County (Nova Scotia), and Charlotte County (New Brunswick). Including temperature in the models improved forecasting ability for lobster catches for Maine and Halifax but not Charlotte. In Maine, lobster landings in year t were related to SST in year t. In Halifax, however, landings were related to SST in the previous 4 yr but not by SST of the current year. Lower fishing effort levels for Halifax compared with Maine probably extended the yield from strong year-classes through a number of years. We surmise that an increase in sea temperature near Halifax during the early 1980's increased the survival and growth rates of lobste...

Lewis S. Incze - One of the best experts on this subject based on the ideXlab platform.