The Experts below are selected from a list of 969 Experts worldwide ranked by ideXlab platform
Conggang Li - One of the best experts on this subject based on the ideXlab platform.
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measurement of amide proton chemical shift anisotropy in perdeuterated proteins using csa amplification
Journal of Magnetic Resonance, 2017Co-Authors: Yuwei Ge, Ivan Hung, Conggang LiAbstract:Abstract Measuring 1 H chemical shift anisotropy (CSA) is useful for probing proton environments and dynamics but remains a challenge due to strong Homonuclear Interaction and relatively small shift anisotropy, especially in proteins with multiple proton sites. Here the extended chemical shift anisotropy amplification (xCSA) method is applied for amide proton CSA measurement in uniformly 2 H enriched proteins under fast magic angle spinning. The xCSA method is capable of distinguishing the sign of the CSA asymmetry parameter, complimenting other multiple-pulse recoupling methods. A three-dimensional xCSA experiment is demonstrated for measuring the proton CSA of amide sites in a GB1 protein sample and the possible correlation of amide proton CSA with protein secondary structure is discussed.
Jerry C. C. Chan - One of the best experts on this subject based on the ideXlab platform.
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Time displacement rotational echo double resonance: Heteronuclear dipolar recoupling with suppression of Homonuclear Interaction under fast magic-angle spinning
Journal of Magnetic Resonance, 2012Co-Authors: Tim W. T. Tsai, Yun Mou, Jerry C. C. ChanAbstract:Abstract We have developed a novel variant of REDOR which is applicable to multiple-spin systems without proton decoupling. The pulse sequence is constructed based on a systematic time displacement of the pi pulses of the conventional REDOR sequence. This so-called time displacement REDOR (td-REDOR) is insensitive to the effect of Homonuclear dipole–dipole Interaction when the higher order effects are negligible. The validity of td-REDOR has been verified experimentally by the P-31{C-13} measurements on glyphosate at a spinning frequency of 25 kHz. The experimental dephasing curve is in favorable agreement with the simulation data without considering the Homonuclear dipole–dipole Interactions.
Yuwei Ge - One of the best experts on this subject based on the ideXlab platform.
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measurement of amide proton chemical shift anisotropy in perdeuterated proteins using csa amplification
Journal of Magnetic Resonance, 2017Co-Authors: Yuwei Ge, Ivan Hung, Conggang LiAbstract:Abstract Measuring 1 H chemical shift anisotropy (CSA) is useful for probing proton environments and dynamics but remains a challenge due to strong Homonuclear Interaction and relatively small shift anisotropy, especially in proteins with multiple proton sites. Here the extended chemical shift anisotropy amplification (xCSA) method is applied for amide proton CSA measurement in uniformly 2 H enriched proteins under fast magic angle spinning. The xCSA method is capable of distinguishing the sign of the CSA asymmetry parameter, complimenting other multiple-pulse recoupling methods. A three-dimensional xCSA experiment is demonstrated for measuring the proton CSA of amide sites in a GB1 protein sample and the possible correlation of amide proton CSA with protein secondary structure is discussed.
Tim W. T. Tsai - One of the best experts on this subject based on the ideXlab platform.
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Time displacement rotational echo double resonance: Heteronuclear dipolar recoupling with suppression of Homonuclear Interaction under fast magic-angle spinning
Journal of Magnetic Resonance, 2012Co-Authors: Tim W. T. Tsai, Yun Mou, Jerry C. C. ChanAbstract:Abstract We have developed a novel variant of REDOR which is applicable to multiple-spin systems without proton decoupling. The pulse sequence is constructed based on a systematic time displacement of the pi pulses of the conventional REDOR sequence. This so-called time displacement REDOR (td-REDOR) is insensitive to the effect of Homonuclear dipole–dipole Interaction when the higher order effects are negligible. The validity of td-REDOR has been verified experimentally by the P-31{C-13} measurements on glyphosate at a spinning frequency of 25 kHz. The experimental dephasing curve is in favorable agreement with the simulation data without considering the Homonuclear dipole–dipole Interactions.
Ivan Hung - One of the best experts on this subject based on the ideXlab platform.
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measurement of amide proton chemical shift anisotropy in perdeuterated proteins using csa amplification
Journal of Magnetic Resonance, 2017Co-Authors: Yuwei Ge, Ivan Hung, Conggang LiAbstract:Abstract Measuring 1 H chemical shift anisotropy (CSA) is useful for probing proton environments and dynamics but remains a challenge due to strong Homonuclear Interaction and relatively small shift anisotropy, especially in proteins with multiple proton sites. Here the extended chemical shift anisotropy amplification (xCSA) method is applied for amide proton CSA measurement in uniformly 2 H enriched proteins under fast magic angle spinning. The xCSA method is capable of distinguishing the sign of the CSA asymmetry parameter, complimenting other multiple-pulse recoupling methods. A three-dimensional xCSA experiment is demonstrated for measuring the proton CSA of amide sites in a GB1 protein sample and the possible correlation of amide proton CSA with protein secondary structure is discussed.