The Experts below are selected from a list of 786 Experts worldwide ranked by ideXlab platform
Rudi F. Vogel - One of the best experts on this subject based on the ideXlab platform.
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Structure/function relationship of Homopolysaccharide producing glycansucrases and therapeutic potential of their synthesised glycans
Applied Microbiology and Biotechnology, 2006Co-Authors: Maher Korakli, Rudi F. VogelAbstract:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have additional medical applications. In the last years, many LAB have been screened for their ability to produce exopoly- and oligosaccharides, and several glycosyltransferases involved in their biosynthesis have been characterised at biochemical and genetic levels. These research efforts aim to exploit the full potential of these organisms and to understand the structure/function relationship of glycosyltransferases. The latter knowledge is a prerequisite for the production of tailored exopoly- and oligosaccharides for the diverse applications. This review will survey the results of recent works on the structure/function relationship of Homopolysaccharide producing glycosyltransferases and the therapeutic potential of their synthesised exopoly- and oligosaccharides.
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structure function relationship of Homopolysaccharide producing glycansucrases and therapeutic potential of their synthesised glycans
Applied Microbiology and Biotechnology, 2006Co-Authors: Maher Korakli, Rudi F. VogelAbstract:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have additional medical applications. In the last years, many LAB have been screened for their ability to produce exopoly- and oligosaccharides, and several glycosyltransferases involved in their biosynthesis have been characterised at biochemical and genetic levels. These research efforts aim to exploit the full potential of these organisms and to understand the structure/function relationship of glycosyltransferases. The latter knowledge is a prerequisite for the production of tailored exopoly- and oligosaccharides for the diverse applications. This review will survey the results of recent works on the structure/function relationship of Homopolysaccharide producing glycosyltransferases and the therapeutic potential of their synthesised exopoly- and oligosaccharides.
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Extracellular Homopolysaccharides and oligosaccharides from intestinal lactobacilli
Journal of Applied Microbiology, 2005Co-Authors: Markus Tieking, Maher Korakli, Rudi F. Vogel, Susanne Kaditzky, Rosica Valcheva, Michael G. GänzleAbstract:Aims: To characterize lactobacilli isolated from the intestines of ducks or pigs with respect to the production of extracellular Homopolysaccharides (HoPS) and oligosaccharides. Methods and Results: Lactobacillus strains of duck or pig origin were screened for HoPS synthesis and >25% of the isolates produced fructans or glucans from sucrose. Glucan-forming strains were found within the species Lactobacillus reuteri and Lactobacillus animalis and fructan-forming strains were found within Lactobacillus mucosae, Lactobacillus crispatus and Lactobacillus acidophilus. The glucan-forming strains of L. reuteri but not L. animalis produced glucose-oligosaccharides in additon to the respective polymers, and two fructan-forming strains of L. acidophilus produced kestose. Genes coding for glycosyltransferases were detected by PCR and partially characterized by sequence analysis. Conclusions: A large proportion of lactobacilli from intestinal habitats produce HoPS from sucrose and polysaccharide formation is generally associated with the formation of glucose- and fructose oligosaccharides. Significance and Impact of the Study: The characterization of the metabolic potential of intestinal lactobacilli contributes to the understanding of the molecular basis of autochthony in intestinal habitats. Moreover, this is the first report of glucose-oligosaccharide production during growth of lactobacilli, and one novel fructosyltransferase and one novel glucansucrase were partially characterized on the genetic level.
Maher Korakli - One of the best experts on this subject based on the ideXlab platform.
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Structure/function relationship of Homopolysaccharide producing glycansucrases and therapeutic potential of their synthesised glycans
Applied Microbiology and Biotechnology, 2006Co-Authors: Maher Korakli, Rudi F. VogelAbstract:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have additional medical applications. In the last years, many LAB have been screened for their ability to produce exopoly- and oligosaccharides, and several glycosyltransferases involved in their biosynthesis have been characterised at biochemical and genetic levels. These research efforts aim to exploit the full potential of these organisms and to understand the structure/function relationship of glycosyltransferases. The latter knowledge is a prerequisite for the production of tailored exopoly- and oligosaccharides for the diverse applications. This review will survey the results of recent works on the structure/function relationship of Homopolysaccharide producing glycosyltransferases and the therapeutic potential of their synthesised exopoly- and oligosaccharides.
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structure function relationship of Homopolysaccharide producing glycansucrases and therapeutic potential of their synthesised glycans
Applied Microbiology and Biotechnology, 2006Co-Authors: Maher Korakli, Rudi F. VogelAbstract:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have additional medical applications. In the last years, many LAB have been screened for their ability to produce exopoly- and oligosaccharides, and several glycosyltransferases involved in their biosynthesis have been characterised at biochemical and genetic levels. These research efforts aim to exploit the full potential of these organisms and to understand the structure/function relationship of glycosyltransferases. The latter knowledge is a prerequisite for the production of tailored exopoly- and oligosaccharides for the diverse applications. This review will survey the results of recent works on the structure/function relationship of Homopolysaccharide producing glycosyltransferases and the therapeutic potential of their synthesised exopoly- and oligosaccharides.
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Extracellular Homopolysaccharides and oligosaccharides from intestinal lactobacilli
Journal of Applied Microbiology, 2005Co-Authors: Markus Tieking, Maher Korakli, Rudi F. Vogel, Susanne Kaditzky, Rosica Valcheva, Michael G. GänzleAbstract:Aims: To characterize lactobacilli isolated from the intestines of ducks or pigs with respect to the production of extracellular Homopolysaccharides (HoPS) and oligosaccharides. Methods and Results: Lactobacillus strains of duck or pig origin were screened for HoPS synthesis and >25% of the isolates produced fructans or glucans from sucrose. Glucan-forming strains were found within the species Lactobacillus reuteri and Lactobacillus animalis and fructan-forming strains were found within Lactobacillus mucosae, Lactobacillus crispatus and Lactobacillus acidophilus. The glucan-forming strains of L. reuteri but not L. animalis produced glucose-oligosaccharides in additon to the respective polymers, and two fructan-forming strains of L. acidophilus produced kestose. Genes coding for glycosyltransferases were detected by PCR and partially characterized by sequence analysis. Conclusions: A large proportion of lactobacilli from intestinal habitats produce HoPS from sucrose and polysaccharide formation is generally associated with the formation of glucose- and fructose oligosaccharides. Significance and Impact of the Study: The characterization of the metabolic potential of intestinal lactobacilli contributes to the understanding of the molecular basis of autochthony in intestinal habitats. Moreover, this is the first report of glucose-oligosaccharide production during growth of lactobacilli, and one novel fructosyltransferase and one novel glucansucrase were partially characterized on the genetic level.
Hiroshi Nakanishi - One of the best experts on this subject based on the ideXlab platform.
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rhamnan sulfate from cell walls of monostroma latissimum
Phytochemistry, 1998Co-Authors: Tohru Yamagaki, Masaakira Maeda, Hiroshi NakanishiAbstract:Abstract Rhamnan sulfate from cell walls of Monostroma latissimum was purified through subsequent chromatographic systems. The purified polysaccharide consisted of large amounts of rhamnose residues and appeared to be an entire Homopolysaccharide. Antithrombin activity was lower than rhamnan sulfate previously obtained from M. nitidum but similar to standard heparin. Studies of the major structural parts of the rhamnan sulfate by periodate oxidation, Smith degradation and permethylation, showed it to consist of 1,3- and 1,2-linked rhamnose residues in a ratio of 3 : 2. Sulfate was mainly substituted at C-3 or C-4 in the 1,2-linked rhamnose residue. The detection of oligosaccharides by matrix-assisted laser desorption\ionization time-of-flight mass spectrometry supports this structure. These results suggested that rhamnan sulfate from the Monostromaceae has quite different structural properties from ulvan from the Ulvaceae.
Masaakira Maeda - One of the best experts on this subject based on the ideXlab platform.
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rhamnan sulfate from cell walls of monostroma latissimum
Phytochemistry, 1998Co-Authors: Tohru Yamagaki, Masaakira Maeda, Hiroshi NakanishiAbstract:Abstract Rhamnan sulfate from cell walls of Monostroma latissimum was purified through subsequent chromatographic systems. The purified polysaccharide consisted of large amounts of rhamnose residues and appeared to be an entire Homopolysaccharide. Antithrombin activity was lower than rhamnan sulfate previously obtained from M. nitidum but similar to standard heparin. Studies of the major structural parts of the rhamnan sulfate by periodate oxidation, Smith degradation and permethylation, showed it to consist of 1,3- and 1,2-linked rhamnose residues in a ratio of 3 : 2. Sulfate was mainly substituted at C-3 or C-4 in the 1,2-linked rhamnose residue. The detection of oligosaccharides by matrix-assisted laser desorption\ionization time-of-flight mass spectrometry supports this structure. These results suggested that rhamnan sulfate from the Monostromaceae has quite different structural properties from ulvan from the Ulvaceae.
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Chemical structure of antithrombin-active rhamnan sulfate from Monostrom nitidum
Bioscience Biotechnology and Biochemistry, 1998Co-Authors: Naoki Harada, Masaakira MaedaAbstract:Rhamnan sulfate was extracted with boiling water from the cell wall of Monostroma nitidum, and the resulting extract was purified by ion-exchange and size-exclusion chromatography. The polysaccharide, which is regarded as a Homopolysaccharide, was 6-fold more antithrombin-active than the heparin standard. The antithrombin activity was decreased, by desulfation, although the non-active product still retained 8% of the sulfate ester. A comparative structural study of the intact rhamnan sulfate and rhamnan, its desulfated product, by periodate oxidation, Smith degradation and methylation analysis revealed the rhamnan sulfate to consist of α-1,3-linked L-rhamnose residues, some of which were substituted with sulfate groups mainly at position O-2. Minor amounts of internal 1,2-linked rhamnose and branched rhamnose linkages were also detected.
Tohru Yamagaki - One of the best experts on this subject based on the ideXlab platform.
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rhamnan sulfate from cell walls of monostroma latissimum
Phytochemistry, 1998Co-Authors: Tohru Yamagaki, Masaakira Maeda, Hiroshi NakanishiAbstract:Abstract Rhamnan sulfate from cell walls of Monostroma latissimum was purified through subsequent chromatographic systems. The purified polysaccharide consisted of large amounts of rhamnose residues and appeared to be an entire Homopolysaccharide. Antithrombin activity was lower than rhamnan sulfate previously obtained from M. nitidum but similar to standard heparin. Studies of the major structural parts of the rhamnan sulfate by periodate oxidation, Smith degradation and permethylation, showed it to consist of 1,3- and 1,2-linked rhamnose residues in a ratio of 3 : 2. Sulfate was mainly substituted at C-3 or C-4 in the 1,2-linked rhamnose residue. The detection of oligosaccharides by matrix-assisted laser desorption\ionization time-of-flight mass spectrometry supports this structure. These results suggested that rhamnan sulfate from the Monostromaceae has quite different structural properties from ulvan from the Ulvaceae.