The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Anthony N. Hollenberg - One of the best experts on this subject based on the ideXlab platform.
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The Nuclear Receptor Corepressor (NCoR) Controls Thyroid Hormone Sensitivity and the Set Point of the Hypothalamic-Pituitary-Thyroid Axis
Molecular Endocrinology, 2011Co-Authors: Inna Astapova, Kristen R. Vella, Kaila A. Holtz, Michael A. Rosenberg, Preeti Ramadoss, Benjamin A. Rodwin, Xiao Hui Liao, Roy E Weiss, Anthony Rosenzweig, Anthony N. HollenbergAbstract:The role of nuclear receptor corepressor (NCoR) in thyroid Hormone (TH) action has been difficult to discern because global deletion of NCoR is embryonic lethal. To circumvent this, we developed mice that globally express a modified NCoR protein (NCoRΔID) that cannot be recruited to the thyroid Hormone receptor (TR). These mice present with low serum T4 and T3 concentrations accompanied by normal TSH levels, suggesting central hypothyroidism. However, they grow normally and have increased energy expenditure and normal or elevated TR-target gene expression across multiple tissues, which is not consistent with hypothyroidism. Although these findings imply an increased peripheral Sensitivity to TH, the hypothalamic-pituitary-thyroid axis is not more sensitive to acute changes in TH concentrations but appears to be reset to recognize the reduced TH levels as normal. Furthermore, the thyroid gland itself, although normal in size, has reduced levels of nonthyroglobulin-bound T4 and T3 and demonstrates decreased responsiveness to TSH. Thus, the TR-NCoR interaction controls systemic TH Sensitivity as well as the set point at all levels of the hypothalamic-pituitary-thyroid axis. These findings suggest that NCoR levels could alter cell-specific TH action that would not be reflected by the serum TSH.
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the nuclear receptor corepressor ncor controls thyroid Hormone Sensitivity and the set point of the hypothalamic pituitary thyroid axis
Endocrine Reviews, 2011Co-Authors: Inna Astapova, Kristen R. Vella, Kaila A. Holtz, Michael A. Rosenberg, Preeti Ramadoss, Benjamin A. Rodwin, Xiao Hui Liao, Roy E Weiss, Anthony Rosenzweig, Anthony N. HollenbergAbstract:This article appears in Molecular Endocrinology, published January 14, 2011, 10.1210/me.2010-0462
Robert Kiss - One of the best experts on this subject based on the ideXlab platform.
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Characterization of steroid Hormone Sensitivity in human breast cancers maintained ex vivo under organotypical culture conditions.
Journal of cancer research and clinical oncology, 2000Co-Authors: Francis Darro, Christine Decaestecker, Isabelle Salmon, Gasto Schwarz, Michel Petein, Sheila Schwarz, Carole Chaboteaux, Jean-marie Nogaret, Robert KissAbstract:Purpose: The methodology we propose combines the immunohistochemical determination of the oestrogen and progesterone receptors (ER and PgR) with the characterization of the oestradiol- and progesterone-induced influence on cell proliferation in breast cancers in order to characterize their steroid Hormone Sensitivity at both the “static” and “dynamic” level. Methods: ER and PgR have been immunohistochemically quantified by means of computer-assisted microscopy. Cell proliferation has been determined by means of tritiated thymidine autoradiography in tumour samples maintained in vitro as organotypic cultures. A series of 14 patients was investigated. Results: Of the 14 breast cancers under study, one with an unequivocally “very ER-rich”/“very PgR-rich” immunohistochemical phenotype totally failed to exhibit any modification in its cell proliferation level after both oestradiol and progesterone stimulation. Two cases definitively associated with an “ER-poor”/“PgR-poor” immunohistochemical phenotype nevertheless responded noticeably to the dynamic stimulation of their cell proliferation by oestradiol and progesterone. While our series of cases covers 14 patients only, it suffices to demonstrate the limits of ER and PgR determination in characterizing steroid Hormone Sensitivity in breast cancer. Discussion: The present work therefore presents an in vitro approach to test growth regulation of human breast cancer by steroid Hormones. The clinical value of the present approach should be further determined by showing that steroid Hormone-induced modifications in cell proliferation level are actually associated with clinical response.
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DIRECT RELATIONSHIP BETWEEN Hormone Sensitivity LEVEL AND GROWTH PATTERN: EVIDENCE IN 18 GASTROINTESTINAL NEOPLASTIC CELL LINES
Analytical and Quantitative Cytology and Histology, 1998Co-Authors: Robert Kiss, Isabelle Camby, Francis Darro, Christine Decaestecker, Isabelle Salmon, Jean-lambert Pasteels, Andre Danguy, Paul YeatonAbstract:OBJECTIVE: We investigated whether a relationship exists in terms of growth pattern and Hormone Sensitivity in 18 gastrointestinal neoplastic cell lines. Hormones studied included gastrin, epidermal growth factor, estradiol and luteinizing Hormone-releasing Hormone. STUDY DESIGN: The growth patterns were assessed by means of computer-assisted microscope analysis of Feulgen-stained nuclei combined with the mathematical Delaunay triangulation and Voronoi paving techniques. This methodology enabled four variables characterizing the cell colony patterns to be computed. The information contributed by these variables was analyzed by means of discriminant analysis and the decision tree technique. RESULTS: Each phenotype (Sensitivity level) exhibited distinct growth pattern (or cell colony) characteristics in the case of each Hormone and/or growth factor under study. Furthermore, the Sensitivity of the gastrointestinal cell lines to a given Hormone (or growth factor) appeared to be peculiar to the Hormone (or growth factor). CONCLUSION: A direct relationship seems to exist between growth pattern and Hormone Sensitivity levels in gastrointestinal cancers, particularly colorectal.
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Ploidy level determination and quantitative chromatin pattern description in pregnancy-associated breast cancers
Breast Cancer Research and Treatment, 1997Co-Authors: Vinicius Budel, Francis Darro, Christine Decaestecker, Isabelle Salmon, Michel Petein, Plinio Gasperin, Sergio Hatschbach, Teresa Cs Cavalcanti, Robert KissAbstract:The present study deals with the characterization of Hormone-Sensitivity in pregnancy-associated breast cancers (PBCs). Thischaracterization was carried out in 22 PBCs as opposed to 88non-pregnancy-associated breast cancers (NPBCs). For this study, we used thedigital cell image analysis of Feulgen-stained nuclei to assess the type ofHormone-Sensitivity. In a previous study it was demonstrated that thechromatin pattern in breast cancers is related to the amounts of estrogenreceptors they contain. Our results demonstrated that the quantitative description of the chromatin pattern by means of 15 parameters (relating tomorphometric, densitometric, and textural features) made it possible toidentify typical cell nuclei populations in the PBC and NPBC groups. Theuse of specific statistical analyses (principal-components anddiscriminant) made it possible to quantify the proportion of each cellnucleus type in the PBCs. Furthermore, of the 22 PBCs under study, 13contained a large majority of cell nuclei whose chromatin pattern wascharacteristic of Hormone-sensitive cells, while 5 cases contained a largemajority of typically Hormone-insensitive ones. The remaining 4 casescontained a relatively similar proportion of typically Hormone-sensitive and insensitive cell nuclei. The quantitative chromatin pattern descriptionthus made it possible to characterize the Hormone-Sensitivity level inPBCs, whereas DNA ploidy level determination did not enable any suchcharacterization to be carried out. The chromatin pattern assay describedhere, which enables Hormone-sensitive pregnancy-associated breast cancersto be identified from Hormone-insensitive ones independently frombiochemical assays, should help the physician regarding therapy adaptation.
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Characterization of the Hormone-Sensitivity of three human non-small-cell lung cancers grafted onto nude mice.
Oncology, 1993Co-Authors: Anna Kruczynski, Jacqueline Astruc, Eric Chazottes, Robert KissAbstract:The in vivo Hormone Sensitivity of three human NSCLCs grafted onto female nude mice (labelled KLX7, KLX9 and KLX14) was characterized on a dynamic level, i.e. on the level of both the macroscopic growth and the proliferative fraction (PF = percentage of cells in the S+G2+M Fractions). Two sets of experiments were performed. The first set showed the influence on the macroscopic growth of the NSCLC xenograft of castration performed either before or after tumor grafting. The second set showed the influence of a pulse of 6 different Hormones or growth factors on the PF index magnitude recorded 36 h after their administration to the xenograft-bearing mice. These 6 Hormones or growth factors were EGF, estradiol-17-beta (E2), gastrin (G), platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF) and bombesin (B). The results show that the KLX7 model grew definitely faster on the nude mice than the other two models. Ovariectomy before or after tumor grafting did not significantly modify the growth pattern of the KLX7 model, while castration before tumor grafting significantly increased the macroscopic growth of both the KLX9 and the KLX14 tumors. In contrast, E2, bFGF, G and B significantly increased the proliferative activity of the KLX7 model 36 h after their administration to the tumor-bearing mice while remaining without any apparent statistically significant effects in both the KLX9 and the KLX14 models.
Inna Astapova - One of the best experts on this subject based on the ideXlab platform.
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The Nuclear Receptor Corepressor (NCoR) Controls Thyroid Hormone Sensitivity and the Set Point of the Hypothalamic-Pituitary-Thyroid Axis
Molecular Endocrinology, 2011Co-Authors: Inna Astapova, Kristen R. Vella, Kaila A. Holtz, Michael A. Rosenberg, Preeti Ramadoss, Benjamin A. Rodwin, Xiao Hui Liao, Roy E Weiss, Anthony Rosenzweig, Anthony N. HollenbergAbstract:The role of nuclear receptor corepressor (NCoR) in thyroid Hormone (TH) action has been difficult to discern because global deletion of NCoR is embryonic lethal. To circumvent this, we developed mice that globally express a modified NCoR protein (NCoRΔID) that cannot be recruited to the thyroid Hormone receptor (TR). These mice present with low serum T4 and T3 concentrations accompanied by normal TSH levels, suggesting central hypothyroidism. However, they grow normally and have increased energy expenditure and normal or elevated TR-target gene expression across multiple tissues, which is not consistent with hypothyroidism. Although these findings imply an increased peripheral Sensitivity to TH, the hypothalamic-pituitary-thyroid axis is not more sensitive to acute changes in TH concentrations but appears to be reset to recognize the reduced TH levels as normal. Furthermore, the thyroid gland itself, although normal in size, has reduced levels of nonthyroglobulin-bound T4 and T3 and demonstrates decreased responsiveness to TSH. Thus, the TR-NCoR interaction controls systemic TH Sensitivity as well as the set point at all levels of the hypothalamic-pituitary-thyroid axis. These findings suggest that NCoR levels could alter cell-specific TH action that would not be reflected by the serum TSH.
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the nuclear receptor corepressor ncor controls thyroid Hormone Sensitivity and the set point of the hypothalamic pituitary thyroid axis
Endocrine Reviews, 2011Co-Authors: Inna Astapova, Kristen R. Vella, Kaila A. Holtz, Michael A. Rosenberg, Preeti Ramadoss, Benjamin A. Rodwin, Xiao Hui Liao, Roy E Weiss, Anthony Rosenzweig, Anthony N. HollenbergAbstract:This article appears in Molecular Endocrinology, published January 14, 2011, 10.1210/me.2010-0462
Kivity Shmuel - One of the best experts on this subject based on the ideXlab platform.
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Safety and value of skin test to sex Hormones and sex Hormone Sensitivity desensitization in women with premenstrual syndrome.
The World Allergy Organization journal, 2019Co-Authors: Alek M. Itsekson, Bomstein Yonit, Zolti Matitiyahu, Kivity ShmuelAbstract:Abstract Background HyperSensitivity to one's own sex Hormones is not a new concept but it is an underappreciated one. Such a phenomenon may explain a large proportion of symptoms related to the menstrual cycle (such as premenstrual syndrome, PMS), cyclic pain syndromes or unexplained infertility. In this study we propose a novel diagnostic tool of hormonal skin testing which reveals sex Hormones Sensitivity with high clinical correlation, and a subsequent successful desensitization technique. Methods A group of 35 women with confirmed diagnosis of PMS were enrolled in the study in which they underwent a hormonal skin diagnostic skin testing procedure by intradermal injections of Progesterone (P), Estradiol (E2), Estrone (E1) and Estriol (E3). Skin reaction was monitored, and according to the reaction the patients were treated by serial desensitization by similar serial injections or placebo solvent. Response to treatment was monitored by assessing the change in the severity of PMS related symptoms. Results We show a positive correlation between PMS severity and skin Sensitivity to sex Hormones. Subsequent desensitization procedure led to a significant improvement in the severity of PMS related symptoms. Conclusions The testing and desensitization procedure is safe, sensitive and bares a high therapeutic potential in approach to resistant hormonal cycle related syndromes. ClinicalTrials.gov Identifier NCT00873262 : Evaluation of Safety/Efficacy of Diagnostic Skin Test Panel and Desensitization Hormone Kit for Treatment of Premenstrual Syndrome (PMS) 2009.
Roy E Weiss - One of the best experts on this subject based on the ideXlab platform.
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The Nuclear Receptor Corepressor (NCoR) Controls Thyroid Hormone Sensitivity and the Set Point of the Hypothalamic-Pituitary-Thyroid Axis
Molecular Endocrinology, 2011Co-Authors: Inna Astapova, Kristen R. Vella, Kaila A. Holtz, Michael A. Rosenberg, Preeti Ramadoss, Benjamin A. Rodwin, Xiao Hui Liao, Roy E Weiss, Anthony Rosenzweig, Anthony N. HollenbergAbstract:The role of nuclear receptor corepressor (NCoR) in thyroid Hormone (TH) action has been difficult to discern because global deletion of NCoR is embryonic lethal. To circumvent this, we developed mice that globally express a modified NCoR protein (NCoRΔID) that cannot be recruited to the thyroid Hormone receptor (TR). These mice present with low serum T4 and T3 concentrations accompanied by normal TSH levels, suggesting central hypothyroidism. However, they grow normally and have increased energy expenditure and normal or elevated TR-target gene expression across multiple tissues, which is not consistent with hypothyroidism. Although these findings imply an increased peripheral Sensitivity to TH, the hypothalamic-pituitary-thyroid axis is not more sensitive to acute changes in TH concentrations but appears to be reset to recognize the reduced TH levels as normal. Furthermore, the thyroid gland itself, although normal in size, has reduced levels of nonthyroglobulin-bound T4 and T3 and demonstrates decreased responsiveness to TSH. Thus, the TR-NCoR interaction controls systemic TH Sensitivity as well as the set point at all levels of the hypothalamic-pituitary-thyroid axis. These findings suggest that NCoR levels could alter cell-specific TH action that would not be reflected by the serum TSH.
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the nuclear receptor corepressor ncor controls thyroid Hormone Sensitivity and the set point of the hypothalamic pituitary thyroid axis
Endocrine Reviews, 2011Co-Authors: Inna Astapova, Kristen R. Vella, Kaila A. Holtz, Michael A. Rosenberg, Preeti Ramadoss, Benjamin A. Rodwin, Xiao Hui Liao, Roy E Weiss, Anthony Rosenzweig, Anthony N. HollenbergAbstract:This article appears in Molecular Endocrinology, published January 14, 2011, 10.1210/me.2010-0462