The Experts below are selected from a list of 12513 Experts worldwide ranked by ideXlab platform
Guang-jin Yuan - One of the best experts on this subject based on the ideXlab platform.
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Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line
International Journal of Molecular Sciences, 2006Co-Authors: He-sheng Luo, Shelley C. Paul, Tao Tang, Guang-jin YuanAbstract:The regulation of glucose and sodium butyrate transporters(glucose transporter1-5 and Monocarboxylate transporter 1) and their relationship with Cell apoptosis induced bysodium butyrate in colonic caner Cell Line HT-29 were studied. Cell apoptosis was detectedby flow cytometric assay. The expression of MCT1 and GLUT1-5 mRNA were detected byRT-PCR and the uptake of glucose was detected using 2-deoxy-[3H]glucose. The expressionof bax and bcl-x/l were detected by westernblot assay. We found that sodium butyrateinduced apoptosis in HT-29 Cell Line. The expression of GLUT1 mRNA, bcl-x/l, as well theuptake of glucose was inhibited by sodium butyrate. The expression of MCT1 and GLUT2,GLUT3, GLUT5 was not regulated by sodium butyrate. However, the concentration ofglucose had positive correlation with the expression of bcl-x/l protein and negativecorrelation with the apoptosis induced by sodium butyrate. All the results suggested thatdownregulation of the expression of GLUT1 was associated with the apoptosis induced bysodium butyrate in HT-29 Cell Line.
Samaneh Khorrami - One of the best experts on this subject based on the ideXlab platform.
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differential microrna expression profile of colon cancer stem Cells derived from primary tumor and ht 29 Cell Line
International Journal of Cancer Management, 2017Co-Authors: Samaneh Khorrami, Ahmad Zavaran Hosseini, Seyed Javad Mowla, Reza GhanbariAbstract:BackgroundColorectal cancer (CRC) is one of the most prevalent cancers in the world. Cancer stem Cells (CSC) have been reported in many human tumors and are thought to be responsible for tumor initiation, therapy resistance, progression, relapse, and metastasis. Recent studies revealed that microRNAs (miRNA) play important roles in maintaining stemness of embryonic stem Cells and CSCs.ObjectivesWith regard to the crucial role of cancer stem Cells in colon cancer initiation and maintenance, the miRNA expression profile in primary and Cell Line cancer stem Cells compared to non-stem Cells cancer Cells were evaluated.MethodsIn this study, a population of colon CSCs from primary colon cancer and human HT-29 colonic adenocarcinoma Cell Line was isolated from serum free medium and their microRNA profiles were evaluated using miRNA PCR array.ResultsThe isolated Cells with high expression of EPCAMﰠ markers showed greater colony-forming efficiency and higher tumorigenic potential. Furthermore, expression of stemness genes including C-myc, Klf4, Nanog, Sox2 and Oct4 was higher in isolated cancer stem Cells. Moreover, miRNA expression profile of colon CSCs was performed using miRNA PCR array. There are 39 differentially expressed miRNAs, 24 of which had lower mean expression in the cancer stem Cells samples. By contrast, 15 miRNAs had higher expression levels in CSCs samples. Of these, miR-495, miR-125, and miR-199a were the most significantly up-regulated miRNAs.ConclusionsOur results suggest that miRNAs might play important roles in maintenance and regulation of colon CSCs and specific miRNA expression signatures may contribute to cancer initiation and expansion.
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Verification of ALDH Activity as a Biomarker in Colon Cancer Stem Cells-Derived HT-29 Cell Line
Iranian journal of cancer prevention, 2015Co-Authors: Samaneh Khorrami, Ahmad Zavaran Hosseini, Seyed Javad Mowla, Reza MalekzadehAbstract:Recent evidence has suggested that epithelial cancers including colorectal cancer (CRC) have driven by a small population of self-renewing, multi-potent Cells termed cancer stem Cells (CSCs) which could be responsible for recurrence of cancer. Aldehyde dehydrogenase 1 (ALDH1) activity has used as a functional stem Cell biomarker to isolate CSCs in different cancers such as colorectal cancer.The main aim of this research was to determine the utility of ALDH1 activity along with CD44 and EPCAM in identifying stem Cell-like Cells in human HT-29 colonic adenocarcinoma Cell Line.In this experimental study, colon CSCs biomarkers including CD44, EPCAM and ALDH1 in colonospheres and parent Cells have analyzed by flow cytometry. The expression levels of stemness genes in spheroid and parental Cells have investigated using SYBR Green real-time PCR. In addition, in vivo xenografts assay has performed to determine tumorigenic potential of tumor spheroid Cells in nude mice.According to results, over 92% of spheroids were CD44+/EpCAM+, while parent Cells only have expressed 38% of CD44/EpCAM biomarkers (P < 0.001). Controversially, ALDH activity was about 2-fold higher in the parent Cells than spheroid Cells (P < 0.05). In comparison with the parental Cells, expression levels of ''stemness'' genes, like Sox2, Oct4, Nanog, C-myc, and Klf4 have significantly increased in colonosphere Cells (P < 0.05). Further, administration of 2500 spheroids could be sufficient to initiate tumor growth in nude mice, while 1x106 of parental Cells has needed to form tumor.For the first time, we have shown that colonospheres with low ALDH1 activity has indicated increased tumorigenic potential and stemness properties. So, it hasn't seemed that ALDH1 could become a useful biomarker to identify CSCs population in HT-29 Cell Line.
He-sheng Luo - One of the best experts on this subject based on the ideXlab platform.
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Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line
International Journal of Molecular Sciences, 2006Co-Authors: He-sheng Luo, Shelley C. Paul, Tao Tang, Guang-jin YuanAbstract:The regulation of glucose and sodium butyrate transporters(glucose transporter1-5 and Monocarboxylate transporter 1) and their relationship with Cell apoptosis induced bysodium butyrate in colonic caner Cell Line HT-29 were studied. Cell apoptosis was detectedby flow cytometric assay. The expression of MCT1 and GLUT1-5 mRNA were detected byRT-PCR and the uptake of glucose was detected using 2-deoxy-[3H]glucose. The expressionof bax and bcl-x/l were detected by westernblot assay. We found that sodium butyrateinduced apoptosis in HT-29 Cell Line. The expression of GLUT1 mRNA, bcl-x/l, as well theuptake of glucose was inhibited by sodium butyrate. The expression of MCT1 and GLUT2,GLUT3, GLUT5 was not regulated by sodium butyrate. However, the concentration ofglucose had positive correlation with the expression of bcl-x/l protein and negativecorrelation with the apoptosis induced by sodium butyrate. All the results suggested thatdownregulation of the expression of GLUT1 was associated with the apoptosis induced bysodium butyrate in HT-29 Cell Line.
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Effect of sodium butyrate on the ex- pression of cathepsins D
2003Co-Authors: He-sheng LuoAbstract:AIM: To detect the effect of sodium butyrate on the expression of cathepsins D(Cath-D) in HT-29 colon carcinoma Cell. METHODS: MTT assay, light microscopy and immunocytochemistry were used to observe the growth, apoptosis and expression of Cath-D in HT-29 Cell Line after treated by sodium butyrate RESULTS: Sodium butyrate inhibited the growth of HT-29 Cell Lines, induced apoptosis and increased the expression of Cath-D in HT-29 Cell Line. CONCLUSION: Sodium butyrate can affect the expressoon of Cath-D and may play an important role in apoptosis of HT-29 Cell Line. Li X, Luo HS, Li F. Effect of sodium butyrate on the expression of cathepsins D. Shijie Huaren Xiaohua Zazhi 2003;11(10):1508-1510
G Zupi - One of the best experts on this subject based on the ideXlab platform.
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N-methylformamide as a potential therapeutic approach in colon cancer.
Diseases of the colon and rectum, 1994Co-Authors: D Del Bufalo, B Bucci, I D'agnano, G ZupiAbstract:The effect of N-methylformamide, used in combination with the antineoplastic drugs adriamycin and cisplatin, on the Cell survival of a colon carcinoma Cell Line (HT-29) was investigated. To better understand the mechanism involved in N-methylformamide-mediated chemosensitization, we evaluated the N-methylformamide effect on Cell volume and surface expression of some integrins molecules (VLA2, VLA5, and VLA6) of the HT-29 Cell Line. The Cell survival was evaluated by clonogenic assay; integrins surface expression was analyzed by means of flow cytometry; Cell volumes were determined using a Coulter Channalyzer. A Noncytotoxic dose of N-methylformamide (170 mM) sensitizes the HT-29 Cell Line to the lethal activity of both adriamycin and cisplatin. The analysis of Cell volume showed that N-methylformamide exposure induces an increase in Cell volume. Flow cytometric analysis of VLA2, VLA5, and VLA6 receptors showed that N-methylformamide increases the expression of the three integrins by 30 to 40 percent. The plasma membrane could constitute one of the N-methylformamide targets and might be involved in the differentiation and chemosensitizing effects caused by this agent. Moreover, N-methylformamide could improve colon cancer treatment when used in combination with antineoplastic drugs.
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N-methylformamide as a potential therapeutic approach in colon cancer.
Diseases of the Colon & Rectum, 1994Co-Authors: D Del Bufalo, B Bucci, I D'agnano, G ZupiAbstract:PURPOSE: The effect of N-methylformamide, used in combination with the antineoplastic drugs adriamycin and cisplatin, on the Cell survival of a colon carcinoma Cell Line (HT-29) was investigated. To better understand the mechanism involved in N-methylformamide-mediated chemosensitization, we evaluated the N-methylformamide effect on Cell volume and surface expression of some integrins molecules (VLA2, VLA5, and VLA6) of the HT-29 Cell Line. METHODS: The Cell survival was evaluated by clonogenic assay; integrins surface expression was analyzed by means of flow cytometry; Cell volumes were determined using a Coulter Channalyzer. RESULTS: A Noncytotoxic dose of N-methylformamide (170 mM) sensitizes the HT-29 Cell Line to the lethal activity of both adriamycin and cisplatin. The analysis of Cell volume showed that N-methylformamide exposure induces an increase in Cell volume. Flow cytometric analysis of VLA2, VLA5, and VLA6 receptors showed that N-methylformamide increases the expression of the three integrins by 30 to 40 percent. CONCLUSION: The plasma membrane could constitute one of the N-methylformamide targets and might be involved in the differentiation and chemosensitizing effects caused by this agent. Moreover,N-methylformamide could improve colon cancer treatment when used in combination with antineoplastic drugs.
Reza Ghanbari - One of the best experts on this subject based on the ideXlab platform.
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differential microrna expression profile of colon cancer stem Cells derived from primary tumor and ht 29 Cell Line
International Journal of Cancer Management, 2017Co-Authors: Samaneh Khorrami, Ahmad Zavaran Hosseini, Seyed Javad Mowla, Reza GhanbariAbstract:BackgroundColorectal cancer (CRC) is one of the most prevalent cancers in the world. Cancer stem Cells (CSC) have been reported in many human tumors and are thought to be responsible for tumor initiation, therapy resistance, progression, relapse, and metastasis. Recent studies revealed that microRNAs (miRNA) play important roles in maintaining stemness of embryonic stem Cells and CSCs.ObjectivesWith regard to the crucial role of cancer stem Cells in colon cancer initiation and maintenance, the miRNA expression profile in primary and Cell Line cancer stem Cells compared to non-stem Cells cancer Cells were evaluated.MethodsIn this study, a population of colon CSCs from primary colon cancer and human HT-29 colonic adenocarcinoma Cell Line was isolated from serum free medium and their microRNA profiles were evaluated using miRNA PCR array.ResultsThe isolated Cells with high expression of EPCAMﰠ markers showed greater colony-forming efficiency and higher tumorigenic potential. Furthermore, expression of stemness genes including C-myc, Klf4, Nanog, Sox2 and Oct4 was higher in isolated cancer stem Cells. Moreover, miRNA expression profile of colon CSCs was performed using miRNA PCR array. There are 39 differentially expressed miRNAs, 24 of which had lower mean expression in the cancer stem Cells samples. By contrast, 15 miRNAs had higher expression levels in CSCs samples. Of these, miR-495, miR-125, and miR-199a were the most significantly up-regulated miRNAs.ConclusionsOur results suggest that miRNAs might play important roles in maintenance and regulation of colon CSCs and specific miRNA expression signatures may contribute to cancer initiation and expansion.