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Mg Cottingham - One of the best experts on this subject based on the ideXlab platform.

  • differential immunogeniCity between hadv 5 and Chimpanzee Adenovirus veCtor Chadox1 is independent of fiber and penton rgd loop sequenCes in miCe
    Scientific Reports, 2015
    Co-Authors: Matthew D J Dicks, Alexandra J Spencer, Lynda Coughlan, Karolis Bauza, Sarah C Gilbert, Adrian V S Hill, Mg Cottingham
    Abstract:

    RepliCation defeCtive Adenoviruses are promising veCtors for the delivery of vaCCine antigens. However, the potential of a veCtor to eliCit transgene-speCifiC adaptive immune responses is largely dependent on the viral serotype used. HAdV-5 (Human Adenovirus C) veCtors are more immunogeniC than Chimpanzee Adenovirus veCtors from speCies Human Adenovirus E (ChAdOx1 and AdC68) in miCe, though the meChanisms responsible for these differenCes in immunogeniCity remain poorly understood. In this study, superior immunogeniCity was assoCiated with markedly higher levels of transgene expression in vivo, partiCularly within draining lymph nodes. To investigate the viral faCtors Contributing to these phenotypes, we generated reCombinant ChAdOx1 veCtors by exChanging Components of the viral Capsid reported to be prinCipally involved in Cell entry with the Corresponding sequenCes from HAdV-5. Remarkably, pseudotyping with the HAdV-5 fiber and/or penton RGD loop had little to no effeCt on in vivo transgene expression or transgene-speCifiC adaptive immune responses despite Considerable speCies-speCifiC sequenCe heterogeneity in these Components. Our results suggest that meChanisms governing veCtor transduCtion after intramusCular administration in miCe may be different from those desCribed in vitro.

  • Differential immunogeniCity between HAdV-5 and Chimpanzee Adenovirus veCtor ChAdOx1 is independent of fiber and penton RGD loop sequenCes in miCe
    'Springer Science and Business Media LLC', 2015
    Co-Authors: Aj Spencer, Coughlan L, Bauza K, Sc Gilbert, Av Hill, Mg Cottingham
    Abstract:

    RepliCation defeCtive Adenoviruses are promising veCtors for the delivery of vaCCine antigens. However, the potential of a veCtor to eliCit transgene-speCifiC adaptive immune responses is largely dependent on the viral serotype used. HAdV-5 (Human Adenovirus C) veCtors are more immunogeniC than Chimpanzee Adenovirus veCtors from speCies Human Adenovirus E (ChAdOx1 and AdC68) in miCe, though the meChanisms responsible for these differenCes in immunogeniCity remain poorly understood. In this study, superior immunogeniCity was assoCiated with markedly higher levels of transgene expression in vivo, partiCularly within draining lymph nodes. To investigate the viral faCtors Contributing to these phenotypes, we generated reCombinant ChAdOx1 veCtors by exChanging Components of the viral Capsid reported to be prinCipally involved in Cell entry with the Corresponding sequenCes from HAdV-5. Remarkably, pseudotyping with the HAdV-5 fiber and/or penton RGD loop had little to no effeCt on in vivo transgene expression or transgene-speCifiC adaptive immune responses despite Considerable speCies-speCifiC sequenCe heterogeneity in these Components. Our results suggest that meChanisms governing veCtor transduCtion after intramusCular administration in miCe may be different from those desCribed in vitro

Henrique Ortêncio Filho - One of the best experts on this subject based on the ideXlab platform.

  • DeteCtion of Adenovirus, papillomavirus and parvovirus in Brazilian bats of the speCies Artibeus lituratus and Sturnira lilium
    Archives of Virology, 2019
    Co-Authors: Fernando...[et Al.] Finoketti, Raíssa Nunes Santos, Aline Alves Scarpellini Campos, André Luís Da Silva Zani, Camila Mosca Barboza, Marcélia Emanuele Sad Fernandes, Tatiane De Cassia Pardo Souza, Driele Delanira Santos, Giovana Werneck Bortolanza, Henrique Ortêncio Filho
    Abstract:

    Bats play a signifiCant role in maintaining their eCosystems through pollination, dispersal of seeds, and Control of inseCt populations, but they are also known to host many miCroorganisms and have been desCribed as natural reservoirs for viruses with zoonotiC potential. The diversity of viruses in these animals remains largely unknown, however, beCause studies are limited by speCies, loCation, virus target, or sample type. Therefore, the aim of this study was to deteCt fragments of viral genomes in bat samples. We performed high-throughput sequenCing analysis and speCifiC PCR and RT-PCR on pools of anal and oropharyngeal swabs from Artibeus lituratus and Sturnira lilium ColleCted in southern Brazil. As a result, a member of the family Adenoviridae related to Human Adenovirus C was deteCted in anal swabs from S. lilium . In addition, we deteCted a papillomavirus in an anal swab from A. lituratus . Our analyses also allowed the deteCtion of Adenoviruses and parvoviruses in oropharyngeal swabs ColleCted from A. lituratus . These results inCrease our knowledge about viral diversity and illustrate the importanCe of ConduCting virus surveillanCe in bats.

Fernando...[et Al.] Finoketti - One of the best experts on this subject based on the ideXlab platform.

  • DeteCtion of Adenovirus, papillomavirus and parvovirus in Brazilian bats of the speCies Artibeus lituratus and Sturnira lilium
    Archives of Virology, 2019
    Co-Authors: Fernando...[et Al.] Finoketti, Raíssa Nunes Santos, Aline Alves Scarpellini Campos, André Luís Da Silva Zani, Camila Mosca Barboza, Marcélia Emanuele Sad Fernandes, Tatiane De Cassia Pardo Souza, Driele Delanira Santos, Giovana Werneck Bortolanza, Henrique Ortêncio Filho
    Abstract:

    Bats play a signifiCant role in maintaining their eCosystems through pollination, dispersal of seeds, and Control of inseCt populations, but they are also known to host many miCroorganisms and have been desCribed as natural reservoirs for viruses with zoonotiC potential. The diversity of viruses in these animals remains largely unknown, however, beCause studies are limited by speCies, loCation, virus target, or sample type. Therefore, the aim of this study was to deteCt fragments of viral genomes in bat samples. We performed high-throughput sequenCing analysis and speCifiC PCR and RT-PCR on pools of anal and oropharyngeal swabs from Artibeus lituratus and Sturnira lilium ColleCted in southern Brazil. As a result, a member of the family Adenoviridae related to Human Adenovirus C was deteCted in anal swabs from S. lilium . In addition, we deteCted a papillomavirus in an anal swab from A. lituratus . Our analyses also allowed the deteCtion of Adenoviruses and parvoviruses in oropharyngeal swabs ColleCted from A. lituratus . These results inCrease our knowledge about viral diversity and illustrate the importanCe of ConduCting virus surveillanCe in bats.

  • DeteCtion of Adenovirus, papillomavirus and parvovirus in Brazilianbats of the speCies Artibeus lituratus and Sturnira lilium
    Springer, 2019
    Co-Authors: Fernando...[et Al.] Finoketti
    Abstract:

    AbstraCtBats play a signifiCant role in maintaining their eCosystems through pollination, dispersal of seeds, and Control of inseCtpopulations, but they are also known to host many miCroorganisms and have been desCribed as natural reservoirs for viruseswith zoonotiC potential. The diversity of viruses in these animals remains largely unknown, however, beCause studies arelimited by speCies, loCation, virus target, or sample type. Therefore, the aim of this study was to deteCt fragments of viralgenomes in bat samples. We performed high-throughput sequenCing analysis and speCifiC PCR and RT-PCR on pools of analand oropharyngeal swabs from Artibeus lituratus and Sturnira lilium ColleCted in southern Brazil. As a result, a member ofthe family Adenoviridae related to Human Adenovirus C was deteCted in anal swabs from S. lilium. In addition, we deteCted apapillomavirus in an anal swab from A. lituratus. Our analyses also allowed the deteCtion of Adenoviruses and parvovirusesin oropharyngeal swabs ColleCted from A. lituratus. These results inCrease our knowledge about viral diversity and illustratethe importanCe of ConduCting virus surveillanCe in bats

Wenjie Tan - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetiC evidenCe for intratypiC reCombinant events in a novel Human Adenovirus C that Causes severe aCute respiratory infeCtion in Children
    Scientific Reports, 2016
    Co-Authors: Yanqun Wang, Yanjie Zhao, Zhengde Xie, Jun Shen, Wenjie Tan
    Abstract:

    Human Adenoviruses (HAdVs) are prevalent in hospitalized Children with severe aCute respiratory infeCtion (SARI). Here, we report a unique reCombinant HAdV strain (CBJ113) isolated from a HAdV-positive Child with SARI. The whole-genome sequenCe was determined using Sanger sequenCing and high-throughput sequenCing. A phylogenetiC analysis of the Complete genome indiCated that the CBJ113 strain shares a Common origin with HAdV-C2, HAdV-C6, HAdV-C1, HAdV-C5, and HAdV-C57 and formed a novel subClade on the same branCh as other HAdV-C subtypes. BootSCan and single nuCleotide polymorphism analyses showed that the CBJ113 genome has an intra-subtype reCombinant struCture and Comprises gene regions mainly originating from two CirCulating viral strains: HAdV-1 and HAdV-2. The parental penton base, pVI, and DBP genes of the reCombinant strain Clustered with the HAdV-1 prototype strain, and the E1B, hexon, fiber, and 100 K genes of the reCombinant Clustered within the HAdV-2 subtype, meanwhile the E4orf1 and DNA polymerase genes of the reCombinant shared the greatest similarity with those of HAdV-5 and HAdV-6, respeCtively. All of these findings provide insight into our understanding of the dynamiCs of the Complexity of the HAdV-C epidemiC. More extensive studies should address the pathogeniCity and CliniCal CharaCteristiCs of the novel reCombinant.

Matthew D J Dicks - One of the best experts on this subject based on the ideXlab platform.

  • differential immunogeniCity between hadv 5 and Chimpanzee Adenovirus veCtor Chadox1 is independent of fiber and penton rgd loop sequenCes in miCe
    Scientific Reports, 2015
    Co-Authors: Matthew D J Dicks, Alexandra J Spencer, Lynda Coughlan, Karolis Bauza, Sarah C Gilbert, Adrian V S Hill, Mg Cottingham
    Abstract:

    RepliCation defeCtive Adenoviruses are promising veCtors for the delivery of vaCCine antigens. However, the potential of a veCtor to eliCit transgene-speCifiC adaptive immune responses is largely dependent on the viral serotype used. HAdV-5 (Human Adenovirus C) veCtors are more immunogeniC than Chimpanzee Adenovirus veCtors from speCies Human Adenovirus E (ChAdOx1 and AdC68) in miCe, though the meChanisms responsible for these differenCes in immunogeniCity remain poorly understood. In this study, superior immunogeniCity was assoCiated with markedly higher levels of transgene expression in vivo, partiCularly within draining lymph nodes. To investigate the viral faCtors Contributing to these phenotypes, we generated reCombinant ChAdOx1 veCtors by exChanging Components of the viral Capsid reported to be prinCipally involved in Cell entry with the Corresponding sequenCes from HAdV-5. Remarkably, pseudotyping with the HAdV-5 fiber and/or penton RGD loop had little to no effeCt on in vivo transgene expression or transgene-speCifiC adaptive immune responses despite Considerable speCies-speCifiC sequenCe heterogeneity in these Components. Our results suggest that meChanisms governing veCtor transduCtion after intramusCular administration in miCe may be different from those desCribed in vitro.