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Mitsuo Oshimura - One of the best experts on this subject based on the ideXlab platform.
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transmission of genomic instability from a single irradiated Human Chromosome to the progeny of unirradiated cells
Radiation Research, 2007Co-Authors: Mitsuo Oshimura, Naoki Mukaida, Seiji Kodama, Keiji Suzuki, Masami WatanabeAbstract:Abstract Mukaida, N., Kodama, S., Suzuki, K., Oshimura, M. and Watanabe, M. Transmission of Genomic Instability from a Single Irradiated Human Chromosome to the Progeny of Unirradiated Cells. Radiat. Res. 167, 675–681 (2007). Ionizing radiation can induce Chromosome instability that is transmitted over many generations after irradiation in the progeny of surviving cells, but it remains unclear why this instability can be transmitted to the progeny. To acquire knowledge about the transmissible nature of genomic instability, we transferred an irradiated Human Chromosome into unirradiated mouse recipient cells by microcell fusion and examined the stability of the transferred Human Chromosome in the microcell hybrids. The transferred Chromosome was stable in all six microcell hybrids in which an unirradiated Human Chromosome had been introduced. In contrast, the transferred Chromosome was unstable in four out of five microcell hybrids in which an irradiated Human Chromosome had been introduced. The aberration...
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Human Chromosome 5 carries a putative telomerase repressor gene.
Genes Chromosomes and Cancer, 2002Co-Authors: Hiroyuki Kugoh, Katsuyo Shigenami, Kenji Funaki, J Carl Barrett, Mitsuo OshimuraAbstract:Telomerase, the ribonucleoprotein enzyme that maintains the telomere, is active in Human germ and stem cells and in a majority of tumor tissues and immortalized cell lines. In contrast, telomerase activity is not detected in most somatic cells, suggesting that normal Human cells contain a regulatory factor(s) to repress this activity. To identify which Human Chromosomes carry a gene or genes that function as telomerase repressors, we investigated telomerase activity in hybrids of the B16-F10 cell line, which contain individual Human Chromosomes transferred previously by microcell fusion and therefore represent a hybrid panel for the entire genome except for the Y Chromosome. Microcell hybrids with an introduced normal Human Chromosome 5 showed inhibition of telomerase activity, but clones at a late passage exhibited reactivation of telomerase activity. Reactivation of telomerase activity was accompanied by deletion and/or rearrangement of the transferred Human Chromosome 5. The introduction of other Human Chromosomes did not significantly affect the telomerase activity of B16-F10 cells. The effect of suppression of telomerase activity in microcell hybrids containing Chromosome 5 was accompanied by a reduction in the level of mTERT mRNA, which encodes a component of the telomerase complex. The putative telomerase repressor gene was mapped to Human Chromosome bands 5p11–p13 by a combination of functional analysis using transfer of subchromosomal transferable fragments of Chromosome 5 into B16-F10 cells and deletion mapping of revertant clones with reactivated telomerase activity. Thus, these results suggest that loss of a gene(s) on this Chromosome was responsible for telomerase reactivation, indicating that Human Chromosome 5 contains a gene or genes that can regulate the expression of mTERT in B16-F10 cells. © 2002 Wiley-Liss, Inc.
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suppression of metastasis of rat prostate cancer by introduction of Human Chromosome 13
Asian Journal of Andrology, 2002Co-Authors: S Hosoki, Naoki Nihei, Mitsuo Oshimura, Hiroyoshi Suzuki, Yayoi Ichikawa, Takeshi Ueda, Yukio Naya, Koichiro Akakura, Tatsuo Igarashi, J C BarrettAbstract:Aim: Chromosome 13 is one of the most frequently altered Chromosomes in prostate cancer. The present study was undertaken to examine the role of Human Chromosome 13 in the progression of prostate cancer. Methods: Human Chromosome 13 was introduced into highly metastatic rat prostate cancer cells via microcell-mediated Chromosome transfer. Results: Microcell hybrid clones containing Human Chromosome 13 showed suppression of metastasis to the lung without any suppression of tumorigenicity, except for one clone, which contained the smallest sized Human Chromosome 13 and did not show any suppression on lung metastasis. Expression of two known tumor suppressor genes, BRCA2 and RB1, which map to Chromosome 13, was examined by reverse transcription- polymerase chain reaction analysis. BRCA2 was expressed only in the metastasis-suppressed microcell-hybrid clones, whereas RB1 was expressed in all clones. Conclusion: Human Chromosome 13 contains metastasis suppressor gene (s) for prostate cancer derived from rat. Furthermore, the RB1 gene is unlikely to be involved in the suppression of metastasis evident in this system.
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Isolation and mapping of 186 new DNA markers on Human Chromosome 1.
Genomics, 1995Co-Authors: Hiroyuki Kugoh, Kota Mitsuya, T Mita, Hiromitsu Uejima, Y. Yuasa, N. Suzuki, Y. Nakagawa, M. Suzuki, Mitsuo OshimuraAbstract:To obtain DNA markers on Human Chromosome 1, we first isolated 500 cosmid clones from mouse A9 cells containing a Human Chromosome 1 tagged with pSV2neo. Of these, 186 were localized on each band of Human Chromosome 1 by R-banding fluorescence in situ hybridization; 118 and 68 were on the short and long arms, respectively. We performed restriction fragment length polymorphism (RFLP) analysis of these cosmid clones, and polymorphism was recognized with one or more enzyme in 43 of them. Two markers proved to have variable numbers of tandem repeats. Since several tumor suppressor genes, as well as genes responsible for hereditary disorders, may be located on this Human Chromosome, the DNA markers will be useful for RFLP analysis or the isolation of new genes related to various disorders.
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suppression of metastasis of rat prostatic cancer by introducing Human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Isamu Hayata, Naoki Nihei, Mitsuo Oshimura, John T. Isaacs, Hiroyoshi Suzuki, Yusuke Nakamura, Jun ShimazakiAbstract:In previous allelotype analyses of Human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of Human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of Human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of Human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that Human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that Human Chromosome 8 has an important role in development of prostatic cancer.
Naoki Nihei - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostate cancer by introduction of Human Chromosome 13
Asian Journal of Andrology, 2002Co-Authors: S Hosoki, Naoki Nihei, Mitsuo Oshimura, Hiroyoshi Suzuki, Yayoi Ichikawa, Takeshi Ueda, Yukio Naya, Koichiro Akakura, Tatsuo Igarashi, J C BarrettAbstract:Aim: Chromosome 13 is one of the most frequently altered Chromosomes in prostate cancer. The present study was undertaken to examine the role of Human Chromosome 13 in the progression of prostate cancer. Methods: Human Chromosome 13 was introduced into highly metastatic rat prostate cancer cells via microcell-mediated Chromosome transfer. Results: Microcell hybrid clones containing Human Chromosome 13 showed suppression of metastasis to the lung without any suppression of tumorigenicity, except for one clone, which contained the smallest sized Human Chromosome 13 and did not show any suppression on lung metastasis. Expression of two known tumor suppressor genes, BRCA2 and RB1, which map to Chromosome 13, was examined by reverse transcription- polymerase chain reaction analysis. BRCA2 was expressed only in the metastasis-suppressed microcell-hybrid clones, whereas RB1 was expressed in all clones. Conclusion: Human Chromosome 13 contains metastasis suppressor gene (s) for prostate cancer derived from rat. Furthermore, the RB1 gene is unlikely to be involved in the suppression of metastasis evident in this system.
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suppression of metastasis of rat prostatic cancer by introducing Human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Isamu Hayata, Naoki Nihei, Mitsuo Oshimura, John T. Isaacs, Hiroyoshi Suzuki, Yusuke Nakamura, Jun ShimazakiAbstract:In previous allelotype analyses of Human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of Human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of Human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of Human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that Human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that Human Chromosome 8 has an important role in development of prostatic cancer.
Hiroyoshi Suzuki - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostate cancer by introduction of Human Chromosome 13
Asian Journal of Andrology, 2002Co-Authors: S Hosoki, Naoki Nihei, Mitsuo Oshimura, Hiroyoshi Suzuki, Yayoi Ichikawa, Takeshi Ueda, Yukio Naya, Koichiro Akakura, Tatsuo Igarashi, J C BarrettAbstract:Aim: Chromosome 13 is one of the most frequently altered Chromosomes in prostate cancer. The present study was undertaken to examine the role of Human Chromosome 13 in the progression of prostate cancer. Methods: Human Chromosome 13 was introduced into highly metastatic rat prostate cancer cells via microcell-mediated Chromosome transfer. Results: Microcell hybrid clones containing Human Chromosome 13 showed suppression of metastasis to the lung without any suppression of tumorigenicity, except for one clone, which contained the smallest sized Human Chromosome 13 and did not show any suppression on lung metastasis. Expression of two known tumor suppressor genes, BRCA2 and RB1, which map to Chromosome 13, was examined by reverse transcription- polymerase chain reaction analysis. BRCA2 was expressed only in the metastasis-suppressed microcell-hybrid clones, whereas RB1 was expressed in all clones. Conclusion: Human Chromosome 13 contains metastasis suppressor gene (s) for prostate cancer derived from rat. Furthermore, the RB1 gene is unlikely to be involved in the suppression of metastasis evident in this system.
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suppression of metastasis of rat prostatic cancer by introducing Human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Isamu Hayata, Naoki Nihei, Mitsuo Oshimura, John T. Isaacs, Hiroyoshi Suzuki, Yusuke Nakamura, Jun ShimazakiAbstract:In previous allelotype analyses of Human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of Human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of Human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of Human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that Human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that Human Chromosome 8 has an important role in development of prostatic cancer.
J C Barrett - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostate cancer by introduction of Human Chromosome 13
Asian Journal of Andrology, 2002Co-Authors: S Hosoki, Naoki Nihei, Mitsuo Oshimura, Hiroyoshi Suzuki, Yayoi Ichikawa, Takeshi Ueda, Yukio Naya, Koichiro Akakura, Tatsuo Igarashi, J C BarrettAbstract:Aim: Chromosome 13 is one of the most frequently altered Chromosomes in prostate cancer. The present study was undertaken to examine the role of Human Chromosome 13 in the progression of prostate cancer. Methods: Human Chromosome 13 was introduced into highly metastatic rat prostate cancer cells via microcell-mediated Chromosome transfer. Results: Microcell hybrid clones containing Human Chromosome 13 showed suppression of metastasis to the lung without any suppression of tumorigenicity, except for one clone, which contained the smallest sized Human Chromosome 13 and did not show any suppression on lung metastasis. Expression of two known tumor suppressor genes, BRCA2 and RB1, which map to Chromosome 13, was examined by reverse transcription- polymerase chain reaction analysis. BRCA2 was expressed only in the metastasis-suppressed microcell-hybrid clones, whereas RB1 was expressed in all clones. Conclusion: Human Chromosome 13 contains metastasis suppressor gene (s) for prostate cancer derived from rat. Furthermore, the RB1 gene is unlikely to be involved in the suppression of metastasis evident in this system.
Jun Shimazaki - One of the best experts on this subject based on the ideXlab platform.
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suppression of metastasis of rat prostatic cancer by introducing Human Chromosome 8
Cancer Research, 1994Co-Authors: Tomohiko Ichikawa, Isamu Hayata, Naoki Nihei, Mitsuo Oshimura, John T. Isaacs, Hiroyoshi Suzuki, Yusuke Nakamura, Jun ShimazakiAbstract:In previous allelotype analyses of Human prostatic cancer specimens, allelic loss on the short arm of Chromosome 8 is frequently observed. However, it is still unclear whether this allelic loss is an initial event or a later one in development of prostatic cancer. Our previous studies demonstrate that introduction of Human Chromosome 11 into highly metastatic rat prostatic cancer cells results in suppression of metastatic ability without suppression of the in vivo growth rate or tumorigenicity of the hybrid cells (T. Ichikawa et al. Cancer Res., 52: 3486–3490, 1992). To clarify the role of Human Chromosome 8 in prostatic cancer, this Chromosome was introduced into highly metastatic rat prostatic cancer cells using microcell-mediated Chromosome transfer. Introduction of Human Chromosome 8 resulted in suppression of metastatic ability of the microcell hybrids, whereas no suppression of the in vivo growth rate or tumorigenicity was observed. These results demonstrate that Human Chromosome 8 contains metastasls suppressor gene(s) for prostatic cancer derived from a rat. These also suggest that Human Chromosome 8 has an important role in development of prostatic cancer.
