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Alfredo Pineyrolopez - One of the best experts on this subject based on the ideXlab platform.
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comparison of the hepatotoxicity of toxin t 514 of karwinskia Humboldtiana and its diastereoisomer in primary liver cell cultures
Toxicon, 1994Co-Authors: Lourdes Garzaocanas, T Jiang, Daniel Acosta, O Torresalanis, Waksman N De Torres, Alfredo PineyrolopezAbstract:Toxin T-514 of Karwinskia Humboldtiana has been demonstrated to be hepatotoxic in vivo and in vitro. Recently a diastereoisomer of T-514 has been isolated. In the present study we have evaluated and compared the in vitro hepatoxicity of the diastereoisomer of T-514 using primary cultures of rat hepatocytes. Cytotoxicity was evaluated by release of cytoplasmic enzyme lactate dehydrogenase (LDH), and mitochondrial metabolic function (MTT reduction). The diastereoisomer was shown to be almost as hepatoxic in vitro as toxin T-514.
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effect of toxins 544 and 514 from karwinskia Humboldtiana buckthorn plant upon fetal development of the mouse
Toxicon, 1993Co-Authors: R Valadezalvizo, A Rodriguezalvarado, Martinez L De Villarreal, Rogerio Gonzalezalanis, Alfredo PineyrolopezAbstract:R. Valadez-Alvizo, A. Rodriguez-Alvarado, L. Martinez de Villarreal, R. Gonzalez-Alanis and A. Pineyro-Lopez. Effect of toxins 544 and 514 from Karwinskia Humboldtiana (buckthorn) plant upon fetal development of the mouse. Toxicon31, 1329–1332, 1993.—The teratogenic effect of toxins 544 and 514 from K. Humboldtiana upon the mouse embryo was evaluated. One half of the ld50 dose for the mouse was administered at day 8 of gestation. At the end of pregnancy, reproduction and fetal data were recorded. Dams treated with toxin 544, but not with toxin 514, showed a higher incidence of reabsorptions, malformations, as well as lower fetal length compared to the control group.
Alfredo Pineyro Lopez - One of the best experts on this subject based on the ideXlab platform.
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frecuencia de intoxicacion con karwinskia Humboldtiana en mexico
Salud Publica De Mexico, 1995Co-Authors: Maria Victoria Bermudez De Rocha, Fabiola Eugenia Lozano Melendez, Victor Armando Tamezrodriguez, Genaro Diaz Cuello, Alfredo Pineyro LopezAbstract:Intoxication produced by Karwinskia Humboldtiana presents a neurological picture similar to that of poliomyelitis,Guillain-Barre syndrome or other polyradiculoneuritis with which it is frequently confused. The purpose of this paper is to report the frequency of this intoxication, by means of the antecedent of ingestion of the fruit and the detection of toxins in blood using a thin layer chromatography method. One hundred fifty four samples of cases with acute flaccid paralysis from 18 states of the country were received. The antecedent of ingestion in 56 of them was corroborated and the detection was positive in 50 of these. In 98 patients there was not antecedent of ingestion and detection was negative in 95 of them. We estimated that the sensibility and specificity of detection method are 89% and 96.9% respectively.
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tissue culture ofkarwinskia Humboldtiana a plant producing toxins with antitumoural effects
Plant Cell Tissue and Organ Culture, 1994Co-Authors: Desana Li Skova, Jose Ruiz Ordonez, Alfredo Pineyro LopezAbstract:Isolated embryos ofKarwinskia Humboldtiana were cultured in vitro. The growth of embryos and development to plantlets on woody plant medium supplemented with indole-3-acetic acid 6.10-2 μmol l−1, gibberellic acid (GA3) 3.10-2 μmol l−1, and 6-benzylaminopurine (BA) 2 μmol l−1 was obtained. Multiplication of shoots and rooting of excised shoots has been achieved. Callus formation on modified Murashige-Skoog medium supplemented with 1-naphthaleneacetic acid 10 μmol l−1, GA3 14 μmol l−1, and kinetin 5 μmol l−1 on hypocotyls, or on root cultures on medium supplemented with 2.4-dichlorophenoxyacetic acid 10 μmol l−1 and BA 10 μmol l−1 was induced.
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Tissue culture ofKarwinskia Humboldtiana—a plant producing toxins with antitumoural effects
Plant Cell Tissue and Organ Culture, 1994Co-Authors: Desana Li Skova, Jose Ruiz Ordonez, Alfredo Pineyro LopezAbstract:Isolated embryos ofKarwinskia Humboldtiana were cultured in vitro. The growth of embryos and development to plantlets on woody plant medium supplemented with indole-3-acetic acid 6.10-2 μmol l−1, gibberellic acid (GA3) 3.10-2 μmol l−1, and 6-benzylaminopurine (BA) 2 μmol l−1 was obtained. Multiplication of shoots and rooting of excised shoots has been achieved. Callus formation on modified Murashige-Skoog medium supplemented with 1-naphthaleneacetic acid 10 μmol l−1, GA3 14 μmol l−1, and kinetin 5 μmol l−1 on hypocotyls, or on root cultures on medium supplemented with 2.4-dichlorophenoxyacetic acid 10 μmol l−1 and BA 10 μmol l−1 was induced.
Maria Victoria Bermudez - One of the best experts on this subject based on the ideXlab platform.
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clinical diagnosis in karwinskia Humboldtiana polyneuropathy
Journal of the Neurological Sciences, 1998Co-Authors: Hector R Martinez, Maria Victoria Bermudez, Ricardo A Rangelguerra, Laura De Leon FloresAbstract:Intoxication by Karwinskia Humboldtiana presents a neurological picture similar to that for Guillain-Barre syndrome or other polyradiculoneuropathies. Clinical diagnosis in poisoned humans may be difficult if no evidence of previous fruit ingestion is available. We present our experience in the clinical diagnosis of Karwinskia Humboldtiana polyneuropathy, as confirmed by toxin detection in blood. We designed an open trial at the Pediatric Neurology service and included all cases with acute ascending paralysis that were admitted to our hospital in the last two years. In all cases, we performed hematological, immunological and biochemical profiles, CSF analysis including immunological studies, oligoclonal bands and myelin basic protein determinations. Electrodiagnostic studies were performed, including motor conduction velocities, distal latencies, F-wave latency and compound muscle action potential (CAMP) amplitude. The presence of Karwinskia Humboldtiana toxins in blood were determined by thin layer chromatography. In six cases, T-514 Karwinskia Humboldtiana toxin was detected. These cases had a symmetric motor polyneuropathy with the absence of tendon reflexes and no sensory signs or cranial nerve involvement. Only one patient required assisted ventilation due to bulbar paralysis. In two of these cases, a sural nerve biopsy revealed a segmental demyelination with swelling and phagocytic chambers in Schwann cells and without lymphocytic infiltration. All six cases survived, with complete recovery in five. We conclude that this intoxication is common in Mexico. The availability of toxin detection in blood samples allows the clinician to establish an accurate diagnosis and should be included in the study of children with polyradiculoneuropathy, especially in countries where this poisonous plant grows.
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frecuencia de intoxicacion con karwinskia Humboldtiana en mexico
Salud Publica De Mexico, 1995Co-Authors: Maria Victoria Bermudez, Fabiola Eugenia Lozano, Victor Armando Tamez, Genaro Diaz, Alfredo PineyroAbstract:Ii~toxication produced by Karwinskia Humboldtiana preseirts a neurological pichm similar to that of poliomielitis, Guillain-Basre qndrome or otherpolyr.adi...
Alfredo Pineyro - One of the best experts on this subject based on the ideXlab platform.
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frecuencia de intoxicacion con karwinskia Humboldtiana en mexico
Salud Publica De Mexico, 1995Co-Authors: Maria Victoria Bermudez, Fabiola Eugenia Lozano, Victor Armando Tamez, Genaro Diaz, Alfredo PineyroAbstract:Ii~toxication produced by Karwinskia Humboldtiana preseirts a neurological pichm similar to that of poliomielitis, Guillain-Basre qndrome or otherpolyr.adi...
Martinez L De Villarreal - One of the best experts on this subject based on the ideXlab platform.
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effect of toxins 544 and 514 from karwinskia Humboldtiana buckthorn plant upon fetal development of the mouse
Toxicon, 1993Co-Authors: R Valadezalvizo, A Rodriguezalvarado, Martinez L De Villarreal, Rogerio Gonzalezalanis, Alfredo PineyrolopezAbstract:R. Valadez-Alvizo, A. Rodriguez-Alvarado, L. Martinez de Villarreal, R. Gonzalez-Alanis and A. Pineyro-Lopez. Effect of toxins 544 and 514 from Karwinskia Humboldtiana (buckthorn) plant upon fetal development of the mouse. Toxicon31, 1329–1332, 1993.—The teratogenic effect of toxins 544 and 514 from K. Humboldtiana upon the mouse embryo was evaluated. One half of the ld50 dose for the mouse was administered at day 8 of gestation. At the end of pregnancy, reproduction and fetal data were recorded. Dams treated with toxin 544, but not with toxin 514, showed a higher incidence of reabsorptions, malformations, as well as lower fetal length compared to the control group.
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effects of toxin t 544 from the karwinskia Humboldtiana buckthorn plant upon mouse embryos explanted at 11 days
Toxicon, 1990Co-Authors: Martinez L De Villarreal, R Velazcocampos, Pineyro A Lopez, Gonzalez R AlanisAbstract:Abstract L. Martinez de Villarreal , R. Velazco-Campos , A. Pineyro Lopez and R. Gonzalez Alanis . Effects of toxin T-544 from the Karwinskia Humboldtiana (buckthorn) plant upon mouse embryos explanted at 11 days. Toxicon 28, 449–452, 1990.—Eleven-day mouse embryos were exposed to the K. Humboldtiana toxin T-544 for 24 hr. At the end of the culture period, embryos were examined grossly for malformations and biochemically for altered protein levels. There was a significant difference in malformations in those embryos exposed to 0.05 and 0.2 μg/ml of toxin compared with controls. Embryo protein content was significantly lower in those embryos exposed to 0.1 μg/ml of T-544 compared with control group.
