The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Nigel Dunn-coleman - One of the best experts on this subject based on the ideXlab platform.
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Transformation of the thermophilic fungus Humicola grisea var. thermoidea and overproduction of Humicola glucoamylase
Current Genetics, 1992Co-Authors: Daniel S. Allison, Michael W. Rey, Randy M. Berka, Gale L. Armstrong, Nigel Dunn-colemanAbstract:The thermophilic fungus Humicola grisea var. thermoidea was successfully transformed using a bleomycin-phleomycin resistance gene linked to regulatory sequences from Aspergillus nidulans. Transformation was achieved using the lithium acetate method with young mycelia, and transformants were obtained at a frequency of 0.5–2 per μg of plasmid DNA. Vector DNA used in transformations was integrated in the genome of Humicola in varying patterns and copy number, and transformants were mitotically stable. Extra copies of an Humicola gla1 gene encoding glucoamylase (GAM) were introduced into the genome of several Humicola strains by transformation, with the result that some transformants produced almost 3-fold more GAM in comparison to the untransformed parental strains.
Daniel S. Allison - One of the best experts on this subject based on the ideXlab platform.
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Transformation of the thermophilic fungus Humicola grisea var. thermoidea and overproduction of Humicola glucoamylase
Current Genetics, 1992Co-Authors: Daniel S. Allison, Michael W. Rey, Randy M. Berka, Gale L. Armstrong, Nigel Dunn-colemanAbstract:The thermophilic fungus Humicola grisea var. thermoidea was successfully transformed using a bleomycin-phleomycin resistance gene linked to regulatory sequences from Aspergillus nidulans. Transformation was achieved using the lithium acetate method with young mycelia, and transformants were obtained at a frequency of 0.5–2 per μg of plasmid DNA. Vector DNA used in transformations was integrated in the genome of Humicola in varying patterns and copy number, and transformants were mitotically stable. Extra copies of an Humicola gla1 gene encoding glucoamylase (GAM) were introduced into the genome of several Humicola strains by transformation, with the result that some transformants produced almost 3-fold more GAM in comparison to the untransformed parental strains.
Gyula Zaray - One of the best experts on this subject based on the ideXlab platform.
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Investigation of the toxic effect of cadmium on Candida Humicola and Bacillus subtilis using a microcalorimetric method.
Journal of hazardous materials, 2008Co-Authors: Hai-yan Chen, Jun Yao, Yong Zhou, Hui-lun Chen, Fei Wang, Nan Gai, Ren-sheng Zhuang, Brunello Ceccanti, Thomas Maskow, Gyula ZarayAbstract:In this study, the technique of microcalorimetry based on heat-output by aerobic bacterial respiration was explored to evaluate the toxic effect of cadmium on Candida Humicola, Bacillus subtilis, singularly or in a mixture of both. Power-time curves of the growth metabolism of C. Humicola and B. subtilis and the effect of Cd(2+) were studied using the TAM III (the third generation thermal activity monitor) multi-channel microcalorimetric system, isothermal mode, at 28 degrees C. The differences in shape of the power-time curves and the thermodynamic and kinetic characteristics of microorganisms growth were compared. The effect of cadmium added into microorganism would significantly reduce the life cycle and change the thermal effect of microbial metabolic process with different concentrations of Cd(2+). The experimental results revealed that at the same concentration, the sequence of inhibitory ratio (I) and maximum thermal power (P(max)) of the Cd(2+) was: mixed microorganisms>C. Humicola>B. subtilis. The sequence of total thermal effect (Q(total)) and growth rate constant (k) is mixed microorganisms>B. subtilis>C. Humicola. These results are important to further studies of the physiology and pharmacology of C. Humicola and B. subtilis and may support the theory of restoring contaminated soil.
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Investigation of the toxic effect of cadmium on Candida Humicola and Bacillus subtilis using a microcalorimetric method
Journal of Hazardous Materials, 2008Co-Authors: Hai-yan Chen, Jun Yao, Yong Zhou, Hui-lun Chen, Fei Wang, Nan Gai, Ren-sheng Zhuang, Brunello Ceccanti, Thomas Maskow, Gyula ZarayAbstract:Abstract In this study, the technique of microcalorimetry based on heat-output by aerobic bacterial respiration was explored to evaluate the toxic effect of cadmium on Candida Humicola , Bacillus subtilis , singularly or in a mixture of both. Power–time curves of the growth metabolism of C. Humicola and B. subtilis and the effect of Cd 2+ were studied using the TAM III (the third generation thermal activity monitor) multi-channel microcalorimetric system, isothermal mode, at 28 °C. The differences in shape of the power–time curves and the thermodynamic and kinetic characteristics of microorganisms growth were compared. The effect of cadmium added into microorganism would significantly reduce the life cycle and change the thermal effect of microbial metabolic process with different concentrations of Cd 2+ . The experimental results revealed that at the same concentration, the sequence of inhibitory ratio ( I ) and maximum thermal power ( P max ) of the Cd 2+ was: mixed microorganisms > C. Humicola > B. subtilis . The sequence of total thermal effect ( Q total ) and growth rate constant ( k ) is mixed microorganisms > B. subtilis > C. Humicola . These results are important to further studies of the physiology and pharmacology of C. Humicola and B. subtilis and may support the theory of restoring contaminated soil.
Swapandeep Singh Chimni - One of the best experts on this subject based on the ideXlab platform.
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Kinetic Resolution of β- and γ-Hydroxy Sulfides by Fungal Lipase from Humicola lanuginosa
Enantiomer, 2002Co-Authors: Satwinder Singh, Subodh Kumar, Swapandeep Singh ChimniAbstract:Racemic g - and n -hydroxy sulfides were resolved by Humicola lanuginosa lipase catalyzed transesterification using vinyl acetate both as acyl donor and solvent. The effect of substituents and spacer length on rate of reaction and enantioselectivity is observed.
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Kinetic resolution of heteroaryl β-hydroxy sulfides catalyzed by Humicola lanuginosa lipase
Tetrahedron: Asymmetry, 2002Co-Authors: Swapandeep Singh Chimni, Satwinder Singh, Subodh Kumar, Savita MahajanAbstract:Abstract Humicola lanuginosa lipase-catalyzed resolution of heteroaryl substituted β-hydroxy sulfides by irreversible transesterification using vinyl acetate as acylating agent is discussed. The ee of the resolved alcohols was determined from the 1 H NMR of their corresponding ( S )-(+)- O -acetylmandelic acid esters.
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Humicola lanuginosa lipase catalyzed enantioselective resolution of β hydroxy sulfides versatile synthons for enantiopure β hydroxy sulfoxides
Tetrahedron-asymmetry, 2001Co-Authors: Satwinder Singh, Subodh Kumar, Swapandeep Singh ChimniAbstract:Abstract Humicola lanuginosa lipase-catalyzed acylation of β-hydroxy sulfides provides both the ( R )- and ( S )-enantiomers in high enantiomeric purity. In two cases the resolved hydroxy sulfides were oxidized to give β-hydroxy sulfoxides in >99% e.e. The effect of substituents on enantioselectivity is discussed.
Randy M. Berka - One of the best experts on this subject based on the ideXlab platform.
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Transformation of the thermophilic fungus Humicola grisea var. thermoidea and overproduction of Humicola glucoamylase
Current Genetics, 1992Co-Authors: Daniel S. Allison, Michael W. Rey, Randy M. Berka, Gale L. Armstrong, Nigel Dunn-colemanAbstract:The thermophilic fungus Humicola grisea var. thermoidea was successfully transformed using a bleomycin-phleomycin resistance gene linked to regulatory sequences from Aspergillus nidulans. Transformation was achieved using the lithium acetate method with young mycelia, and transformants were obtained at a frequency of 0.5–2 per μg of plasmid DNA. Vector DNA used in transformations was integrated in the genome of Humicola in varying patterns and copy number, and transformants were mitotically stable. Extra copies of an Humicola gla1 gene encoding glucoamylase (GAM) were introduced into the genome of several Humicola strains by transformation, with the result that some transformants produced almost 3-fold more GAM in comparison to the untransformed parental strains.