The Experts below are selected from a list of 71964 Experts worldwide ranked by ideXlab platform
Caroline Costedoat - One of the best experts on this subject based on the ideXlab platform.
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cross species amplification of 41 microsatellites in european cyprinids a tool for evolutionary population genetics and Hybridization Studies
BMC Research Notes, 2010Co-Authors: Vincent Dubut, André Gilles, Rémi Chappaz, Jean-francois Martin, Melthide Sinama, Emese Meglecz, Juliette Fernandez, Caroline CostedoatAbstract:Background Cyprinids display the most abundant and widespread species among the European freshwater Teleostei and are known to hybridize quite commonly. Nevertheless, a limited number of markers for conducting comparative differentiation, evolutionary and Hybridization dynamics Studies are available to date.
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Cross-species amplification of 41 microsatellites in European cyprinids: A tool for evolutionary, population genetics and Hybridization Studies
BMC Research Notes, 2010Co-Authors: Vincent Dubut, André Gilles, Rémi Chappaz, Jean-francois Martin, Melthide Sinama, Emese Meglecz, Juliette Fernandez, Caroline CostedoatAbstract:Background: Cyprinids display the most abundant and widespread species among the European freshwater Teleostei and are known to hybridize quite commonly. Nevertheless, a limited number of markers for conducting comparative differentiation, evolutionary and Hybridization dynamics Studies are available to date. Findings: Five multiplex PCR sets were optimized in order to assay 41 cyprinid-specific polymorphic microsatellite loci (including 10 novel loci isolated from Chondrostoma nasus nasus, Chondrostoma toxostoma toxostoma and Leuciscus leuciscus) for 503 individuals (440 purebred specimens and 63 hybrids) from 15 European cyprinid species. The level of genetic diversity was assessed in Alburnus alburnus, Alburnoides bipunctatus, C. genei, C. n. nasus, C. soetta, C. t. toxostoma, L. idus, L. leuciscus, Pachychilon pictum, Rutilus rutilus, Squalius cephalus and Telestes souffia. The applicability of the markers was also tested on Abramis brama, Blicca bjoerkna and Scardinius erythrophtalmus specimens. Overall, between 24 and 37 of these markers revealed polymorphic for the investigated species and 23 markers amplified for all the 15 European cyprinid species. Conclusions: The developed set of markers demonstrated its performance in discriminating European cyprinid species. Furthermore, it allowed detecting and characterizing hybrid individuals. These microsatellites will therefore be useful to perform comparative evolutionary and population genetics Studies dealing with European cyprinids, what is of particular interest in conservation issues and constitutes a tool of choice to conduct Hybridization Studies.
Mitsunori Yagame - One of the best experts on this subject based on the ideXlab platform.
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in situ Hybridization Studies of matrix metalloproteinase 3 tissue inhibitor of metalloproteinase 1 and type iv collagen in diabetic nephropathy
Kidney International, 1997Co-Authors: Daisuke Suzuki, Masanobu Miyazaki, Kiichiro Jinde, Takehiko Koji, Mitsunori YagameAbstract:In situ Hybridization Studies of matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-1 and type IV collagen in diabetic nephropathy. Progressive expansion of the mesangial matrix is one of the most characteristic histological features of diabetic nephropathy (DN). To determine the balance between the turnover and degradation of extracellular matrix (ECM) in renal tissue of patients with DN, we examined the expression of matrix metalloproteinase-3 (MMP-3), tissue inhibitor of metalloproteinase-1 (TIMP-1) and type IV collagen (IV-C) mRNAs using a high-resolution in situ Hybridization. Patients were divided into three grades: mild (grade I), moderate (grade II) and severe (grade III) mesangial expansion and tubulointerstitial injury. The relationship between the expression of these mRNAs and degree of glomerular mesangial expansion and interstitial injury was also examined. Cells positive for each mRNA were observed in glomerular resident cells, including glomerular mesangial, epithelial and endothelial cells and cells of Bowman's capsule. A number of tubular epithelial cells and some infiltrating cells in the interstitium also expressed these mRNAs. The expression of MMP-3 mRNA and TIMP-1 mRNA was strongest in glomeruli of grade I and inversely correlated with mesangial expansion. In contrast, the expression of all three types of mRNA was correlated with the degree of interstitial injury. Our results indicate that IV-C, MMP-3 and TIMP-1 mRNAs are expressed in glomerular resident cells, tubular epithelial cells and infiltrating cells in renal tissue of DN, and suggest that their expression changes with the degree of mesangial expansion and interstitial injury. Altered expression of MMP-3 and TIMP-1 may be associated with the progression of DN.
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in situ Hybridization Studies of matrix metalloproteinase 3 tissue inhibitor of metalloproteinase 1 and type iv collagen in diabetic nephropathy
The Renal Association. Meeting, 1997Co-Authors: Daisuke Suzuki, Masanobu Miyazaki, Kiichiro Jinde, Takehiko Koji, Mitsunori YagameAbstract:Progressive expansion of the mesangial matrix is one of the most characteristic histological features of diabetic nephropathy (DN). To determine the balance between the turnover and degradation of extracellular matrix (ECM) in renal tissue of patients with DN, we examined the expression of matrix metalloproteinase-3 (MMP-3), tissue inhibitor of metalloproteinase-1 (TIMP-1) and type IV collagen (IV-C) mRNAs using a high-resolution in situ Hybridization. Patients were divided into three grades: mild (grade I), moderate (grade II) and severe (grade III) mesangial expansion and tubulointerstitial injury. The relationship between the expression of these mRNAs and degree of glomerular mesangial expansion and interstitial injury was also examined. Cells positive for each mRNA were observed in glomerular resident cells, including glomerular mesangial, epithelial and endothelial cells and cells of Bowman's capsule. A number of tubular epithelial cells and some infiltrating cells in the interstitium also expressed these mRNAs. The expression of MMP-3 mRNA and TIMP-1 mRNA was strongest in glomeruli of grade I and inversely correlated with mesangial expansion. In contrast, the expression of all three types of mRNA was correlated with the degree of interstitial injury. Our results indicate that IV-C, MMP-3 and TIMP-1 mRNAs are expressed in glomerular resident cells, tubular epithelial cells and infiltrating cells in renal tissue of DN, and suggest that their expression changes with the degree of mesangial expansion and interstitial injury. Altered expression of MMP-3 and TIMP-1 may be associated with the progression of DN.
Gary W Saunders - One of the best experts on this subject based on the ideXlab platform.
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an evaluation of methods used to assess intergeneric Hybridization in kelp using pacific laminariales phaeophyceae
Journal of Phycology, 2005Co-Authors: Louis D Druehl, Julie D Collins, Christopher E Lane, Gary W SaundersAbstract:Kelp intergeneric laminarialean Hybridizations and Hybridization protocol were assessed using seven northeast Pacific kelp species: Alaria marginata Postels and Ruprecht, Costaria costata (C. A. Agardh) Saunders, Eisenia arborea Areschoug, Laminaria saccharina (L) Lamouroux, Lessoniopsis littoralis (Tilden) Reinke, Macrocystis integrifolia Bory, and Nereocystis leutkeana (Mertens) Postels and Ruprecht. Survival and development of sporophyte morphologies derived from selfings, separate males and females, and reciprocal crosses were evaluated over 30 weeks- of cultivation. All cultures were initiated from cloned gametophytes. Two closely related species, Laminaria angustata Kjellman and L. japonica Areschoug, demonstrated the efficacy of long-term (up to 30 years) cloned gametophytes in Hybridization Studies. Sporophyte morphologies appeared in 34%-69% of control and Hybridization trials, and 6%-16% of all trials produced sporophytes in control and Hybridization conditions that persisted through 30 weeks of cultivation. Sporophytes in control and Hybridization conditions could appear normal or abnormal. Usually, the morphology of sporophytes in Hybridizations and female controls resembled the female parent, whereas the sporophytes in male controls often had an abbreviated morphology, lacking definitive generic features. Species-specific rDNA internal transcribed spacer molecular primers were used to determine the parentage of five putative hybrids. Only the L. japonica♀/L. angustata ♂ hybrid bore both parental genomes. That negative controls could produce persistent and normal-appearing sporophytes negates their value and emphasizes the importance of molecular confirmation in Hybridization Studies. These findings were applied to critique the only known wild intergeneric hybrid, Pelagophycus/Macrocystis.
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an evaluation of methods used to assess intergeneric Hybridization in kelp using pacific laminariales phaeophyceae
Journal of Phycology, 2005Co-Authors: Louis D Druehl, Julie D Collins, Christopher E Lane, Gary W SaundersAbstract:Kelp intergeneric laminarialean Hybridizations and Hybridization protocol were assessed using seven northeast Pacific kelp species: Alaria marginata Postels and Ruprecht, Costaria costata (C. A. Agardh) Saunders, Eisenia arborea Areschoug, Laminaria saccharina (L) Lamouroux, Lessoniopsis littoralis (Tilden) Reinke, Macrocystis integrifolia Bory, and Nereocystis leutkeana (Mertens) Postels and Ruprecht. Survival and development of sporophyte morphologies derived from selfings, separate males and females, and reciprocal crosses were evaluated over 30 weeks- of cultivation. All cultures were initiated from cloned gametophytes. Two closely related species, Laminaria angustata Kjellman and L. japonica Areschoug, demonstrated the efficacy of long-term (up to 30 years) cloned gametophytes in Hybridization Studies. Sporophyte morphologies appeared in 34%-69% of control and Hybridization trials, and 6%-16% of all trials produced sporophytes in control and Hybridization conditions that persisted through 30 weeks of cultivation. Sporophytes in control and Hybridization conditions could appear normal or abnormal. Usually, the morphology of sporophytes in Hybridizations and female controls resembled the female parent, whereas the sporophytes in male controls often had an abbreviated morphology, lacking definitive generic features. Species-specific rDNA internal transcribed spacer molecular primers were used to determine the parentage of five putative hybrids. Only the L. japonica♀/L. angustata ♂ hybrid bore both parental genomes. That negative controls could produce persistent and normal-appearing sporophytes negates their value and emphasizes the importance of molecular confirmation in Hybridization Studies. These findings were applied to critique the only known wild intergeneric hybrid, Pelagophycus/Macrocystis.
Jesper Wengel - One of the best experts on this subject based on the ideXlab platform.
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unlocked nucleic acids with a pyrene modified uracil synthesis Hybridization Studies fluorescent properties and i motif stability
ChemBioChem, 2014Co-Authors: Kasper K Karlsen, Pavla Perlikova, Erik B Pedersen, Jesper WengelAbstract:The synthesis of two new phosphoramidite building blocks for the incorporation of 5-(pyren-1-yl)uracilyl unlocked nucleic acid (UNA) monomers into oligonucleotides has been developed. Monomers containing a pyrene-modified nucleobase component were found to destabilize an i-motif structure at pH 5.2, both under molecular crowding and noncrowding conditions. The presence of the pyrene-modified UNA monomers in DNA strands led to decreases in the thermal stabilities of DNA*/DNA and DNA*/RNA duplexes, but these duplexes' thermal stabilities were better than those of duplexes containing unmodified UNA monomers. Pyrene-modified UNA monomers incorporated in bulges were able to stabilize DNA*/DNA duplexes due to intercalation of the pyrene moiety into the duplexes. Steady-state fluorescence emission Studies of oligonucleotides containing pyrene-modified UNA monomers revealed decreases in fluorescence intensities upon Hybridization to DNA or RNA. Efficient quenching of fluorescence of pyrene-modified UNA monomers was observed after formation of i-motif structures at pH 5.2. The stabilizing/destabilizing effect of pyrene-modified nucleic acids might be useful for designing antisense oligonucleotides and Hybridization probes.
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synthesis and Hybridization Studies of 2 amino α l lna and tetracyclic locked lna
Journal of Organic Chemistry, 2006Co-Authors: Santhosh T Kumar, Jesper Wengel, Andreas Stahl Madsen, Patrick J HrdlickaAbstract:A convergent route to a new class of locked nucleic acids, i.e., 2‘-amino-α-L-LNA, has been developed. The optimized synthetic route to the corresponding phosphoramidite building block of thymine proceeds in 4% overall yield over 15 steps from the starting diol. Crucial synthetic steps include (a) introduction of a C2-azido group prior to nucleobase coupling, (b) Vorbruggen glycosylation primarily affording the desired α-anomer, (c) separation of α-l-ribo- and β-l-ribo-configured bicyclic nucleosides, and (d) selection of a suitable protecting group to avoid intramolecular Michael addition of the C2‘-amino group onto the C6-position. Incorporation of a 2‘-amino-α-L-LNA monomer into oligodeoxyribonucleotides results in modest changes in thermal stability with complementary DNA, whereas significant increases in thermal stability are observed with RNA complements along with excellent Watson−Crick discrimination. These results, along with the flexibility of the synthetic strategy allowing chemoselective N2‘-f...
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xylo configured oligonucleotides xna xylo nucleic acids synthesis and Hybridization Studies
ChemInform, 2004Co-Authors: Nicolai E Poopeiko, Britta M Dahl, Jesper WengelAbstract:Abstract We report synthesis and high-affinity Hybridization of fully modified home-thymine 2′-deoxy and 2′-deoxy-2′-fluoro xylo nucleic acids.
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the adenine derivative of α l lna α l ribo configured locked nucleic acid synthesis and high affinity Hybridization towards dna rna lna and α l lna complementary sequences
Bioorganic & Medicinal Chemistry Letters, 2001Co-Authors: Anders E Hakansson, Jesper WengelAbstract:Synthesis of a 9-mer α-l-LNA (α-l-ribo configured locked nucleic acid) containing three 9-(2-O,4-C-methylene-α-l-ribofuranosyl)adenine nucleotide monomer(s) has been accomplished. The work involved synthesis of the bicyclic adenine nucleoside via a condensation reaction between l-threo-pentofuranose derivative 1 and 6-N-benzoyladenine followed by C2′-epimerization. Hybridization Studies demonstrated very strong duplex formation with 9-mer complementary DNA, RNA, LNA and α-l-LNA target sequences.
Vincent Dubut - One of the best experts on this subject based on the ideXlab platform.
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cross species amplification of 41 microsatellites in european cyprinids a tool for evolutionary population genetics and Hybridization Studies
BMC Research Notes, 2010Co-Authors: Vincent Dubut, André Gilles, Rémi Chappaz, Jean-francois Martin, Melthide Sinama, Emese Meglecz, Juliette Fernandez, Caroline CostedoatAbstract:Background Cyprinids display the most abundant and widespread species among the European freshwater Teleostei and are known to hybridize quite commonly. Nevertheless, a limited number of markers for conducting comparative differentiation, evolutionary and Hybridization dynamics Studies are available to date.
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Cross-species amplification of 41 microsatellites in European cyprinids: A tool for evolutionary, population genetics and Hybridization Studies
BMC Research Notes, 2010Co-Authors: Vincent Dubut, André Gilles, Rémi Chappaz, Jean-francois Martin, Melthide Sinama, Emese Meglecz, Juliette Fernandez, Caroline CostedoatAbstract:Background: Cyprinids display the most abundant and widespread species among the European freshwater Teleostei and are known to hybridize quite commonly. Nevertheless, a limited number of markers for conducting comparative differentiation, evolutionary and Hybridization dynamics Studies are available to date. Findings: Five multiplex PCR sets were optimized in order to assay 41 cyprinid-specific polymorphic microsatellite loci (including 10 novel loci isolated from Chondrostoma nasus nasus, Chondrostoma toxostoma toxostoma and Leuciscus leuciscus) for 503 individuals (440 purebred specimens and 63 hybrids) from 15 European cyprinid species. The level of genetic diversity was assessed in Alburnus alburnus, Alburnoides bipunctatus, C. genei, C. n. nasus, C. soetta, C. t. toxostoma, L. idus, L. leuciscus, Pachychilon pictum, Rutilus rutilus, Squalius cephalus and Telestes souffia. The applicability of the markers was also tested on Abramis brama, Blicca bjoerkna and Scardinius erythrophtalmus specimens. Overall, between 24 and 37 of these markers revealed polymorphic for the investigated species and 23 markers amplified for all the 15 European cyprinid species. Conclusions: The developed set of markers demonstrated its performance in discriminating European cyprinid species. Furthermore, it allowed detecting and characterizing hybrid individuals. These microsatellites will therefore be useful to perform comparative evolutionary and population genetics Studies dealing with European cyprinids, what is of particular interest in conservation issues and constitutes a tool of choice to conduct Hybridization Studies.
