The Experts below are selected from a list of 1512 Experts worldwide ranked by ideXlab platform
Susan Bane - One of the best experts on this subject based on the ideXlab platform.
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benzocoumarin Hydrazine a large stokes shift fluorogenic sensor for detecting carbonyls in isolated biomolecules and in live cells
ACS Sensors, 2017Co-Authors: Kamalika Mukherjee, Tak Ian Chio, Abhijit Banerjee, Anthony M Sorrentino, Dan L Sackett, Susan BaneAbstract:Detection and quantification of biomolecule carbonylation, a critical manifestation of oxidative stress, allows better understanding of associated disease states. Existing approaches for such analyses require further processing of cells and tissues, which leads to loss of both spatial and temporal information about carbonylated biomolecules in cells. Live cell detection of these species requires sensors that are nontoxic, sufficiently reactive with the biocarbonyl in the intracellular milieu, and detectable with commonly available instrumentation. Presented here is a new fluorescent sensor for biomolecule carbonyl detection: a Hydrazine Derivative of a benzocoumarin, 7-hydrazinyl-4-methyl-2H-benzo[h]chromen-2-one (BzCH), which meets these requirements. This probe is especially well suited for live cell studies. It can be excited by a laser line common to many fluorescence microscopes. The emission maximum of BzCH undergoes a substantial red shift upon hydrazone formation (from ∼430 to ∼550 nm), which is t...
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Benzocoumarin Hydrazine: A Large Stokes Shift Fluorogenic Sensor for Detecting Carbonyls in Isolated Biomolecules and in Live Cells
2017Co-Authors: Kamalika Mukherjee, Tak Ian Chio, Abhijit Banerjee, Anthony M Sorrentino, Dan L Sackett, Susan BaneAbstract:Detection and quantification of biomolecule carbonylation, a critical manifestation of oxidative stress, allows better understanding of associated disease states. Existing approaches for such analyses require further processing of cells and tissues, which leads to loss of both spatial and temporal information about carbonylated biomolecules in cells. Live cell detection of these species requires sensors that are nontoxic, sufficiently reactive with the biocarbonyl in the intracellular milieu, and detectable with commonly available instrumentation. Presented here is a new fluorescent sensor for biomolecule carbonyl detection: a Hydrazine Derivative of a benzocoumarin, 7-hydrazinyl-4-methyl-2H-benzo[h]chromen-2-one (BzCH), which meets these requirements. This probe is especially well suited for live cell studies. It can be excited by a laser line common to many fluorescence microscopes. The emission maximum of BzCH undergoes a substantial red shift upon hydrazone formation (from ∼430 to ∼550 nm), which is the result of fluorophore disaggregation. Additionally, the hydrazone exhibits an exceptionally large Stokes shift (∼195 nm). The latter properties eliminate self-quenching of the probe and the need to remove unreacted fluorophore for reliable carbonyl detection. Thus, biomolecule carbonylation can be detected and quantified in cells and in cell extracts in a one-step procedure using this probe
Seham Y Hassan - One of the best experts on this subject based on the ideXlab platform.
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synthesis antibacterial and antifungal activity of some new pyrazoline and pyrazole Derivatives
Molecules, 2013Co-Authors: Seham Y HassanAbstract:A series of 2-pyrazolines 5-9 have been synthesized from α,β-unsaturated ketones 2-4. New 2-pyrazoline Derivatives 13-15 bearing benzenesulfonamide moieties were then synthesized by condensing the appropriate chalcones 2-4 with 4-hydrazinyl benzenesulfonamide hydrochloride. Ethyl [1,2,4] triazolo[3,4-c][1,2,4]triazino[5,6-b]-5H-indole-5-ethanoate (26) and 1-(5H-[1,2,4]triazino[5,6-b] indol-3-yl)-3-methyl-1H-pyrazol-5(4H)-one (32) were synthesized from 3-hydrazinyl-5H-[1,2,4]triazino[5,6-b]indole (24). On the other hand ethyl[1,2,4]triazolo[3,4-c][1,2,4]triazino[5,6-b]-5,10-dihydroquinoxaline- 5-ethanoate (27) and 1-(5,10-dihydro-[1,2,4]triazino[5,6-b]quinoxalin-3-yl)-3-methyl-1H-pyrazol-5(4H)-one (33) were synthesized from 3-hydrazinyl-5,10-dihydro-[1,2,4]triazino[5,6-b]quinoxaline (25) by reaction with diethyl malonate or ethyl acetoacetate, respectively. Condensation of 6,6-dimethyl-4-oxo-4,5,6,7-tetrahydro-1H-indole-2-carbaldehyde (1') with compound 24 or 25 afforded the corresponding Schiff's bases 36 and 37, respectively. Reaction of the Schiff's base 37 with benzoyl Hydrazine or acetic anhydride afforded benzohydrazide Derivative 39 and the cyclized compound 40, respectively. Furthermore, the pyrazole Derivatives 42-44 were synthesized by cyclization of Hydrazine Derivative 25 with the prepared chalcones 2-4. All the newly synthesized compounds have been characterized on the basis of IR and 1H-NMR spectral data as well as physical data. Antimicrobial activity against the organisms E. coli ATCC8739 and P. aeruginosa ATCC 9027 as examples of Gram-negative bacteria, S. aureus ATCC 6583P as an example of Gram-positive bacteria and C. albicans ATCC 2091 as an example of a yeast-like fungus have been studied using the Nutrient Agar (NA) and Sabouraud Dextrose Agar (SDA) diffusion methods. The best performance was found for the compounds 16, 17, 19 and 20.
Urmila H Patel - One of the best experts on this subject based on the ideXlab platform.
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crystal structure and hirshfeld surface analysis of methyl 4 e 2 5 bromo 2 meth oxy benzyl idene hydrazin yl 3 nitro benzoate
Acta Crystallographica Section E: Crystallographic Communications, 2018Co-Authors: Tanvirbanu J. Malek, Sahaj A. Gandhi, Vijay Barot, Mukesh Patel, Urmila H PatelAbstract:: The title compound, C16H14BrN3O5, is a novel halogen (Br) substituted Hydrazine Derivative. The Hydrazine Derivatives were the group of compounds with the general structure, R1R2C=NNH2 (Uppal et al., 2011 ▸), with the central RC=NNH2 moiety bridging two different groups on both sides. An all-trans configuration of the backbone (RC=NNH2) results in an extended mol-ecular conformation. The dihedral angle between the 5-bromo-2-meth-oxy-phenyl ring and the nitrophenyl ring is 4.4 (3)°. Intra-molecular N-H⋯O inter-actions form S(6) graph-set motifs, while C-H⋯O and C-H⋯N inter-actions form S(5) graph-set motifs. Symmetry-related mol-ecules are linked by C-H⋯O inter-molecular inter-actions forming an R21(10) graph-set motif. There are nearly face-to-face directional specific π-π stacking inter-actions between the centroids of the nitrophenyl ring and the benzene ring of the 5-bromo-2-meth-oxy group [centroid-centroid distance = 3.6121 (5) A and slippage = 1.115 A], which also contributes to the mol-ecular packing. The Hirshfeld surface analysis was performed in order to visualize, explore and qu-antify the inter-molecular inter-actions in the crystal lattice of the title compound.
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Crystal structure and Hirshfeld surface analysis of methyl 4-[(E)-2-(5-bromo-2-methoxybenzylidene)hydrazinyl]-3-nitrobenzoate
International Union of Crystallography, 2018Co-Authors: Tanvirbanu J. Malek, Sahaj A. Gandhi, Vijay Barot, Mukesh Patel, Urmila H PatelAbstract:The title compound, C16H14BrN3O5, is a novel halogen (Br) substituted Hydrazine Derivative. The Hydrazine Derivatives were the group of compounds with the general structure, R1R2C=NNH2 (Uppal et al., 2011), with the central RC=NNH2 moiety bridging two different groups on both sides. An all-trans configuration of the backbone (RC=NNH2) results in an extended molecular conformation. The dihedral angle between the 5-bromo-2-methoxyphenyl ring and the nitrophenyl ring is 4.4 (3)°. Intramolecular N—H...O interactions form S(6) graph-set motifs, while C—H...O and C—H...N interactions form S(5) graph-set motifs. Symmetry-related molecules are linked by C—H...O intermolecular interactions forming an R21(10) graph-set motif. There are nearly face-to-face directional specific π–π stacking interactions between the centroids of the nitrophenyl ring and the benzene ring of the 5-bromo-2-methoxy group [centroid–centroid distance = 3.6121 (5) Å and slippage = 1.115 Å], which also contributes to the molecular packing. The Hirshfeld surface analysis was performed in order to visualize, explore and quantify the intermolecular interactions in the crystal lattice of the title compound
Kamalika Mukherjee - One of the best experts on this subject based on the ideXlab platform.
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benzocoumarin Hydrazine a large stokes shift fluorogenic sensor for detecting carbonyls in isolated biomolecules and in live cells
ACS Sensors, 2017Co-Authors: Kamalika Mukherjee, Tak Ian Chio, Abhijit Banerjee, Anthony M Sorrentino, Dan L Sackett, Susan BaneAbstract:Detection and quantification of biomolecule carbonylation, a critical manifestation of oxidative stress, allows better understanding of associated disease states. Existing approaches for such analyses require further processing of cells and tissues, which leads to loss of both spatial and temporal information about carbonylated biomolecules in cells. Live cell detection of these species requires sensors that are nontoxic, sufficiently reactive with the biocarbonyl in the intracellular milieu, and detectable with commonly available instrumentation. Presented here is a new fluorescent sensor for biomolecule carbonyl detection: a Hydrazine Derivative of a benzocoumarin, 7-hydrazinyl-4-methyl-2H-benzo[h]chromen-2-one (BzCH), which meets these requirements. This probe is especially well suited for live cell studies. It can be excited by a laser line common to many fluorescence microscopes. The emission maximum of BzCH undergoes a substantial red shift upon hydrazone formation (from ∼430 to ∼550 nm), which is t...
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Benzocoumarin Hydrazine: A Large Stokes Shift Fluorogenic Sensor for Detecting Carbonyls in Isolated Biomolecules and in Live Cells
2017Co-Authors: Kamalika Mukherjee, Tak Ian Chio, Abhijit Banerjee, Anthony M Sorrentino, Dan L Sackett, Susan BaneAbstract:Detection and quantification of biomolecule carbonylation, a critical manifestation of oxidative stress, allows better understanding of associated disease states. Existing approaches for such analyses require further processing of cells and tissues, which leads to loss of both spatial and temporal information about carbonylated biomolecules in cells. Live cell detection of these species requires sensors that are nontoxic, sufficiently reactive with the biocarbonyl in the intracellular milieu, and detectable with commonly available instrumentation. Presented here is a new fluorescent sensor for biomolecule carbonyl detection: a Hydrazine Derivative of a benzocoumarin, 7-hydrazinyl-4-methyl-2H-benzo[h]chromen-2-one (BzCH), which meets these requirements. This probe is especially well suited for live cell studies. It can be excited by a laser line common to many fluorescence microscopes. The emission maximum of BzCH undergoes a substantial red shift upon hydrazone formation (from ∼430 to ∼550 nm), which is the result of fluorophore disaggregation. Additionally, the hydrazone exhibits an exceptionally large Stokes shift (∼195 nm). The latter properties eliminate self-quenching of the probe and the need to remove unreacted fluorophore for reliable carbonyl detection. Thus, biomolecule carbonylation can be detected and quantified in cells and in cell extracts in a one-step procedure using this probe
Yinlong Guo - One of the best experts on this subject based on the ideXlab platform.
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carbon nanotubes 2 5 dihydroxybenzoyl Hydrazine Derivative as ph adjustable enriching reagent and matrix for maldi analysis of trace peptides
Journal of the American Society for Mass Spectrometry, 2006Co-Authors: Shifang Ren, Yinlong GuoAbstract:A functionalized carbon nanotube (CNT), CNT 2,5-dihydroxybenzoyl Hydrazine Derivative, was synthesized and used as both pH adjustable enriching reagent and matrix in matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis of trace peptides. The Derivative reagent, 2,5-dihydroxybenzoyl Hydrazine, introduced phenolic hydroxyl and phenyl groups to the surface of the CNT. The former group can provide adjustable surface charge and a source of protons for chemical ionization, and the latter helps to keep strong ultraviolet absorption for enhancing pulsed laser desorption and ionization. It was found that the functionalized CNT was less twisted in a basic condition (pH 10.5), which afforded an increased surface area to volume ratio for adsorption towards trace peptides. However, functionalized CNT becomes deposited in an acidic condition (pH 5) and can be isolated readily from the sample solutions once the nanoparticles have trapped the target analytes, thus providing a novel and convenient alternative method for quick isolation. Compared with the previously reported method on enriching analytes using the pristine CNT, it is observed that the detection limit for analytes can be greatly improved due to enhancing adsorption capacity of the functionalized CNT. Moreover, peptide mixture at concentration as low as 0.01 pg/µL still can be detected after enrichment mediated by the functionalized CNT, while it is difficult to be detected without enrichment at concentration 0.1 pg/µL using α-cyano-4-hydroxycinnamic acid (CHCA) as matrix. Therefore, high efficiency of adsorption and enrichment towards trace peptides can be achieved by adjusting pH value of the functionalized CNT dispersion.
