The Experts below are selected from a list of 6489 Experts worldwide ranked by ideXlab platform
Nathalie Rochet - One of the best experts on this subject based on the ideXlab platform.
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Ectopic bone formation using an injectable biphasic calcium phosphate/Si-HPMC Hydrogel Composite loaded with undifferentiated bone marrow stromal cells
Biomaterials, 2006Co-Authors: Christophe Trojani, Florian Boukhechba, Jean-claude Scimeca, Fanny Vandenbos, Jean-françois Michiels, Guy Daculsi, Pascal Boileau, Pierre Weiss, Georges Carle, Nathalie RochetAbstract:We have used a new synthetic injectable Composite constituted of hydroxyapatite/tricalcium phosphate (HA/TCP) particles in suspension in a self-hardening Si-hydroxypropylmethylcellulose (HPMC) Hydrogel. The aim of this study was to evaluate in vivo the biocompatibility and the new bone formation efficacy of this scaffold loaded with undifferentiated bone marrow stromal cells (BMSCs). This biomaterial was mixed extemporaneously with BMSCs prepared from C57BL/6 mice, injected in subcutaneous and intramuscular sites and retrieved 4 and 8 weeks after implantation. Dissection of the implants revealed a hard consistency and the absence of a fibrous capsule reflecting a good integration into the host tissues. Histological analysis showed mineralized woven bone in the granule inter-space with numerous active osteoclasts attached to the particles as assessed by the presence of multinucleated cells positively stained for TRAP activity and for the a3 subunit of the V-ATPase. Small vessels were homogenously distributed in the whole implants. Similar results were obtained in SC and IM sites and no bone formation was observed in the control groups when cell-free and particle-free transplants were injected. These results indicate that this injectable biphasic calcium phosphate-Hydrogel Composite mixed with undifferentiated BMSCs is a new promising osteoinductive bone substitute. It also provides with an original in vivo model of osteoclast differentiation and function.
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ectopic bone formation using an injectable biphasic calcium phosphate si hpmc Hydrogel Composite loaded with undifferentiated bone marrow stromal cells
Biomaterials, 2006Co-Authors: Christophe Trojani, Florian Boukhechba, Jean-claude Scimeca, Fanny Vandenbos, Jean-françois Michiels, Guy Daculsi, Pascal Boileau, Pierre Weiss, Georges F Carle, Nathalie RochetAbstract:We have used a new synthetic injectable Composite constituted of hydroxyapatite/tricalcium phosphate (HA/TCP) particles in suspension in a self-hardening Si–hydroxypropylmethylcellulose (HPMC) Hydrogel. The aim of this study was to evaluate in vivo the biocompatibility and the new bone formation efficacy of this scaffold loaded with undifferentiated bone marrow stromal cells (BMSCs). This biomaterial was mixed extemporaneously with BMSCs prepared from C57BL/6 mice, injected in subcutaneous and intramuscular sites and retrieved 4 and 8 weeks after implantation. Dissection of the implants revealed a hard consistency and the absence of a fibrous capsule reflecting a good integration into the host tissues. Histological analysis showed mineralized woven bone in the granule inter-space with numerous active osteoclasts attached to the particles as assessed by the presence of multinucleated cells positively stained for TRAP activity and for the a3 subunit of the V-ATPase. Small vessels were homogenously distributed in the whole implants. Similar results were obtained in SC and IM sites and no bone formation was observed in the control groups when cell-free and particle-free transplants were injected. These results indicate that this injectable biphasic calcium phosphate–Hydrogel Composite mixed with undifferentiated BMSCs is a new promising osteoinductive bone substitute. It also provides with an original in vivo model of osteoclast differentiation and function.
Rocky S. Tuan - One of the best experts on this subject based on the ideXlab platform.
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multilayered polycaprolactone gelatin fiber Hydrogel Composite for tendon tissue engineering
Acta Biomaterialia, 2016Co-Authors: Guang Yang, Hang Lin, Benjamin B. Rothrauff, Rocky S. TuanAbstract:Abstract Regeneration of injured tendon and ligament (T&L) remains a clinical challenge due to their poor intrinsic healing capacity. Tissue engineering provides a promising alternative treatment approach to facilitate T&L healing and regeneration. Successful tendon tissue engineering requires the use of three-dimensional (3D) biomimetic scaffolds that possess the physical and biochemical features of native tendon tissue. We report here the development and characterization of a novel Composite scaffold fabricated by co-electrospinning of poly-e-caprolactone (PCL) and methacrylated gelatin (mGLT). We found that photocrosslinking retained mGLT, resulted in a uniform distribution of mGLT throughout the depth of scaffold and also preserved scaffold mechanical strength. Moreover, photocrosslinking was able to integrate stacked scaffold sheets to form multilayered constructs that mimic the structure of native tendon tissues. Importantly, cells impregnated into the constructs remained responsive to topographical cues and exogenous tenogenic factors, such as TGF-β3. The excellent biocompatibility and highly integrated structure of the scaffold developed in this study will allow the creation of a more advanced tendon graft that possesses the architecture and cell phenotype of native tendon tissues. Statement of Significance The clinical challenges in tendon repair have spurred the development of tendon tissue engineering approaches to create functional tissue replacements. In this study, we have developed a novel Composite scaffold as a tendon graft consisting of aligned poly-e-caprolactone (PCL) microfibers and methacrylated gelatin (mGLT). Cell seeding and photocrosslinking between scaffold layers can be performed simultaneously to create cell impregnated multilayered constructs. This cell-scaffold construct combines the advantages of PCL nanofibrous scaffolds and photocrosslinked gelatin Hydrogels to mimic the structure, mechanical anisotropy, and cell phenotype of native tendon tissue. The scaffold engineered here as a building block for multilayer constructs should have applications beyond tendon tissue engineering in the fabrication of tissue grafts that consist of both fibrous and Hydrogel components.
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Multilayered polycaprolactone/gelatin fiber-Hydrogel Composite for tendon tissue engineering.
Acta biomaterialia, 2016Co-Authors: Guang Yang, Hang Lin, Benjamin B. Rothrauff, Rocky S. TuanAbstract:Abstract Regeneration of injured tendon and ligament (T&L) remains a clinical challenge due to their poor intrinsic healing capacity. Tissue engineering provides a promising alternative treatment approach to facilitate T&L healing and regeneration. Successful tendon tissue engineering requires the use of three-dimensional (3D) biomimetic scaffolds that possess the physical and biochemical features of native tendon tissue. We report here the development and characterization of a novel Composite scaffold fabricated by co-electrospinning of poly-e-caprolactone (PCL) and methacrylated gelatin (mGLT). We found that photocrosslinking retained mGLT, resulted in a uniform distribution of mGLT throughout the depth of scaffold and also preserved scaffold mechanical strength. Moreover, photocrosslinking was able to integrate stacked scaffold sheets to form multilayered constructs that mimic the structure of native tendon tissues. Importantly, cells impregnated into the constructs remained responsive to topographical cues and exogenous tenogenic factors, such as TGF-β3. The excellent biocompatibility and highly integrated structure of the scaffold developed in this study will allow the creation of a more advanced tendon graft that possesses the architecture and cell phenotype of native tendon tissues. Statement of Significance The clinical challenges in tendon repair have spurred the development of tendon tissue engineering approaches to create functional tissue replacements. In this study, we have developed a novel Composite scaffold as a tendon graft consisting of aligned poly-e-caprolactone (PCL) microfibers and methacrylated gelatin (mGLT). Cell seeding and photocrosslinking between scaffold layers can be performed simultaneously to create cell impregnated multilayered constructs. This cell-scaffold construct combines the advantages of PCL nanofibrous scaffolds and photocrosslinked gelatin Hydrogels to mimic the structure, mechanical anisotropy, and cell phenotype of native tendon tissue. The scaffold engineered here as a building block for multilayer constructs should have applications beyond tendon tissue engineering in the fabrication of tissue grafts that consist of both fibrous and Hydrogel components.
Zhiyong Qian - One of the best experts on this subject based on the ideXlab platform.
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injectable and thermo sensitive peg pcl peg copolymer collagen n ha Hydrogel Composite for guided bone regeneration
Biomaterials, 2012Co-Authors: Beiyu Wang, Bingyang Chu, Lan Zheng, Feng Luo, Jingcong Luo, Zhiyong QianAbstract:A novel three-component biomimetic Hydrogel Composite was successfully prepared in this study, which was composed of triblock PEG-PCL-PEG copolymer (PECE), collagen and nano-hydroxyapatite (n-HA). The microstructure and thermo-responsibility of the obtained PECE/Collagen/n-HA Hydrogel Composite were characterized. Scanning electronic microscopy (SEM) showed that the Composite exhibited an interconnected porous structure. The rheological analysis revealed that the Composite existed good thermo-sensitivity. In vivo biocompatibility and biodegradability was investigated by implanting the Hydrogel Composite in muscle pouches of rats for 3, 7, and 14 days. Moreover, the osteogenic capacity was evaluated by means of implanting the Composite material in cranial defects of New Zealand White rabbits for 4, 12 and 20 weeks. In vivo performances confirmed that the biodegradable PECE/Collagen/n-HA Hydrogel Composite had good biocompatibility and better performance in guided bone regeneration than the self-healing process. Thus the thermal-response PECE/Collagen/n-HA Hydrogel Composite had the great potential in bone tissue engineering.
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Injectable and thermo-sensitive PEG-PCL-PEG copolymer/collagen/n-HA Hydrogel Composite for guided bone regeneration.
Biomaterials, 2012Co-Authors: Beiyu Wang, Bingyang Chu, Lan Zheng, Feng Luo, Jingcong Luo, Zhiyong QianAbstract:A novel three-component biomimetic Hydrogel Composite was successfully prepared in this study, which was composed of triblock PEG-PCL-PEG copolymer (PECE), collagen and nano-hydroxyapatite (n-HA). The microstructure and thermo-responsibility of the obtained PECE/Collagen/n-HA Hydrogel Composite were characterized. Scanning electronic microscopy (SEM) showed that the Composite exhibited an interconnected porous structure. The rheological analysis revealed that the Composite existed good thermo-sensitivity. In vivo biocompatibility and biodegradability was investigated by implanting the Hydrogel Composite in muscle pouches of rats for 3, 7, and 14 days. Moreover, the osteogenic capacity was evaluated by means of implanting the Composite material in cranial defects of New Zealand White rabbits for 4, 12 and 20 weeks. In vivo performances confirmed that the biodegradable PECE/Collagen/n-HA Hydrogel Composite had good biocompatibility and better performance in guided bone regeneration than the self-healing process. Thus the thermal-response PECE/Collagen/n-HA Hydrogel Composite had the great potential in bone tissue engineering.
Christophe Trojani - One of the best experts on this subject based on the ideXlab platform.
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Ectopic bone formation using an injectable biphasic calcium phosphate/Si-HPMC Hydrogel Composite loaded with undifferentiated bone marrow stromal cells
Biomaterials, 2006Co-Authors: Christophe Trojani, Florian Boukhechba, Jean-claude Scimeca, Fanny Vandenbos, Jean-françois Michiels, Guy Daculsi, Pascal Boileau, Pierre Weiss, Georges Carle, Nathalie RochetAbstract:We have used a new synthetic injectable Composite constituted of hydroxyapatite/tricalcium phosphate (HA/TCP) particles in suspension in a self-hardening Si-hydroxypropylmethylcellulose (HPMC) Hydrogel. The aim of this study was to evaluate in vivo the biocompatibility and the new bone formation efficacy of this scaffold loaded with undifferentiated bone marrow stromal cells (BMSCs). This biomaterial was mixed extemporaneously with BMSCs prepared from C57BL/6 mice, injected in subcutaneous and intramuscular sites and retrieved 4 and 8 weeks after implantation. Dissection of the implants revealed a hard consistency and the absence of a fibrous capsule reflecting a good integration into the host tissues. Histological analysis showed mineralized woven bone in the granule inter-space with numerous active osteoclasts attached to the particles as assessed by the presence of multinucleated cells positively stained for TRAP activity and for the a3 subunit of the V-ATPase. Small vessels were homogenously distributed in the whole implants. Similar results were obtained in SC and IM sites and no bone formation was observed in the control groups when cell-free and particle-free transplants were injected. These results indicate that this injectable biphasic calcium phosphate-Hydrogel Composite mixed with undifferentiated BMSCs is a new promising osteoinductive bone substitute. It also provides with an original in vivo model of osteoclast differentiation and function.
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ectopic bone formation using an injectable biphasic calcium phosphate si hpmc Hydrogel Composite loaded with undifferentiated bone marrow stromal cells
Biomaterials, 2006Co-Authors: Christophe Trojani, Florian Boukhechba, Jean-claude Scimeca, Fanny Vandenbos, Jean-françois Michiels, Guy Daculsi, Pascal Boileau, Pierre Weiss, Georges F Carle, Nathalie RochetAbstract:We have used a new synthetic injectable Composite constituted of hydroxyapatite/tricalcium phosphate (HA/TCP) particles in suspension in a self-hardening Si–hydroxypropylmethylcellulose (HPMC) Hydrogel. The aim of this study was to evaluate in vivo the biocompatibility and the new bone formation efficacy of this scaffold loaded with undifferentiated bone marrow stromal cells (BMSCs). This biomaterial was mixed extemporaneously with BMSCs prepared from C57BL/6 mice, injected in subcutaneous and intramuscular sites and retrieved 4 and 8 weeks after implantation. Dissection of the implants revealed a hard consistency and the absence of a fibrous capsule reflecting a good integration into the host tissues. Histological analysis showed mineralized woven bone in the granule inter-space with numerous active osteoclasts attached to the particles as assessed by the presence of multinucleated cells positively stained for TRAP activity and for the a3 subunit of the V-ATPase. Small vessels were homogenously distributed in the whole implants. Similar results were obtained in SC and IM sites and no bone formation was observed in the control groups when cell-free and particle-free transplants were injected. These results indicate that this injectable biphasic calcium phosphate–Hydrogel Composite mixed with undifferentiated BMSCs is a new promising osteoinductive bone substitute. It also provides with an original in vivo model of osteoclast differentiation and function.
Guang Yang - One of the best experts on this subject based on the ideXlab platform.
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multilayered polycaprolactone gelatin fiber Hydrogel Composite for tendon tissue engineering
Acta Biomaterialia, 2016Co-Authors: Guang Yang, Hang Lin, Benjamin B. Rothrauff, Rocky S. TuanAbstract:Abstract Regeneration of injured tendon and ligament (T&L) remains a clinical challenge due to their poor intrinsic healing capacity. Tissue engineering provides a promising alternative treatment approach to facilitate T&L healing and regeneration. Successful tendon tissue engineering requires the use of three-dimensional (3D) biomimetic scaffolds that possess the physical and biochemical features of native tendon tissue. We report here the development and characterization of a novel Composite scaffold fabricated by co-electrospinning of poly-e-caprolactone (PCL) and methacrylated gelatin (mGLT). We found that photocrosslinking retained mGLT, resulted in a uniform distribution of mGLT throughout the depth of scaffold and also preserved scaffold mechanical strength. Moreover, photocrosslinking was able to integrate stacked scaffold sheets to form multilayered constructs that mimic the structure of native tendon tissues. Importantly, cells impregnated into the constructs remained responsive to topographical cues and exogenous tenogenic factors, such as TGF-β3. The excellent biocompatibility and highly integrated structure of the scaffold developed in this study will allow the creation of a more advanced tendon graft that possesses the architecture and cell phenotype of native tendon tissues. Statement of Significance The clinical challenges in tendon repair have spurred the development of tendon tissue engineering approaches to create functional tissue replacements. In this study, we have developed a novel Composite scaffold as a tendon graft consisting of aligned poly-e-caprolactone (PCL) microfibers and methacrylated gelatin (mGLT). Cell seeding and photocrosslinking between scaffold layers can be performed simultaneously to create cell impregnated multilayered constructs. This cell-scaffold construct combines the advantages of PCL nanofibrous scaffolds and photocrosslinked gelatin Hydrogels to mimic the structure, mechanical anisotropy, and cell phenotype of native tendon tissue. The scaffold engineered here as a building block for multilayer constructs should have applications beyond tendon tissue engineering in the fabrication of tissue grafts that consist of both fibrous and Hydrogel components.
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Multilayered polycaprolactone/gelatin fiber-Hydrogel Composite for tendon tissue engineering.
Acta biomaterialia, 2016Co-Authors: Guang Yang, Hang Lin, Benjamin B. Rothrauff, Rocky S. TuanAbstract:Abstract Regeneration of injured tendon and ligament (T&L) remains a clinical challenge due to their poor intrinsic healing capacity. Tissue engineering provides a promising alternative treatment approach to facilitate T&L healing and regeneration. Successful tendon tissue engineering requires the use of three-dimensional (3D) biomimetic scaffolds that possess the physical and biochemical features of native tendon tissue. We report here the development and characterization of a novel Composite scaffold fabricated by co-electrospinning of poly-e-caprolactone (PCL) and methacrylated gelatin (mGLT). We found that photocrosslinking retained mGLT, resulted in a uniform distribution of mGLT throughout the depth of scaffold and also preserved scaffold mechanical strength. Moreover, photocrosslinking was able to integrate stacked scaffold sheets to form multilayered constructs that mimic the structure of native tendon tissues. Importantly, cells impregnated into the constructs remained responsive to topographical cues and exogenous tenogenic factors, such as TGF-β3. The excellent biocompatibility and highly integrated structure of the scaffold developed in this study will allow the creation of a more advanced tendon graft that possesses the architecture and cell phenotype of native tendon tissues. Statement of Significance The clinical challenges in tendon repair have spurred the development of tendon tissue engineering approaches to create functional tissue replacements. In this study, we have developed a novel Composite scaffold as a tendon graft consisting of aligned poly-e-caprolactone (PCL) microfibers and methacrylated gelatin (mGLT). Cell seeding and photocrosslinking between scaffold layers can be performed simultaneously to create cell impregnated multilayered constructs. This cell-scaffold construct combines the advantages of PCL nanofibrous scaffolds and photocrosslinked gelatin Hydrogels to mimic the structure, mechanical anisotropy, and cell phenotype of native tendon tissue. The scaffold engineered here as a building block for multilayer constructs should have applications beyond tendon tissue engineering in the fabrication of tissue grafts that consist of both fibrous and Hydrogel components.
