The Experts below are selected from a list of 35115 Experts worldwide ranked by ideXlab platform
Jason A Spector - One of the best experts on this subject based on the ideXlab platform.
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therapeutic delivery of Hydrogen Sulfide for salvage of ischemic skeletal muscle after the onset of critical ischemia
Journal of Vascular Surgery, 2011Co-Authors: Peter W Henderson, N Jimenez, J Ruffino, Allie M Sohn, Andrew L Weinstein, David D Krijgh, Alyssa J Reiffel, Jason A SpectorAbstract:Background Recent evidence suggests that Hydrogen Sulfide is capable of mitigating the degree of cellular damage associated with ischemia-reperfusion injury (IRI). Methods This study evaluated the potential utility of Hydrogen Sulfide in preventing IRI in skeletal muscle by using in vitro (cultured myotubes subjected to sequential hypoxia and normoxia) and in vivo (mouse hind limb ischemia, followed by reperfusion) models to determine whether intravenous Hydrogen Sulfide delivered after the ischemic event had occurred (pharmacologic postconditioning) conferred protection against IRI. Injury score and apoptotic index were determined by analysis of specimens stained with hematoxylin and eosin and terminal deoxynucleotide transferase-mediated deoxy-uridine triphosphate nick-end labeling, respectively. Results In vitro, Hydrogen Sulfide reduced the apoptotic index after 1, 3, or 5 hours of hypoxia by as much as 75% (P = .002), 80% (P = .006), and 83% (P Conclusions These findings confirm that Hydrogen Sulfide limits IRI-induced cellular damage in myotubes and skeletal muscle, even when delivered after the onset of ischemia in this murine model. These data suggest that when given in the appropriate dose and within the proper time frame, Hydrogen Sulfide may have significant therapeutic applications in multiple clinical scenarios.
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therapeutic metabolic inhibition Hydrogen Sulfide significantly mitigates skeletal muscle ischemia reperfusion injury in vitro and in vivo
Plastic and Reconstructive Surgery, 2010Co-Authors: Peter W Henderson, Andrew L Weinstein, David D Krijgh, Sunil P Singh, Vijay Nagineni, Daniel C Rafii, Daniel J Kadouch, Jason A SpectorAbstract:Background: Recent evidence suggests that Hydrogen Sulfide is capable of mitigating the degree of cellular damage associated with ischemia-reperfusion injury. The purpose of this study was to determine whether it is protective in skeletal muscle. Methods: This study used both in vitro (cultured myotubes subjected to sequential anoxia and normoxia) and in vivo (mouse hind-limb ischemia followed by reperfusion) models in which Hydrogen Sulfide (0 to 1000 M) was delivered before the onset of oxygen deficiency. Injury score and apoptotic index were determined by analysis of specimens stained with hematoxylin and eosin and terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling, respectively. Results: In vitro, Hydrogen Sulfide reduced the apoptotic index by as much as 99 percent (p 0.001), with optimal protection conferred by raising intravascular Hydrogen Sulfide to 10 M. In vivo, 10 M Hydrogen Sulfide delivered before 3 hours of hind-limb ischemia followed by 3 hours of reperfusion resulted in protection against ischemia-reperfusion injury–induced cellular changes, as evidenced by significant decreases in injury score and apoptotic index (by as much as 91 percent; p 0.001). These findings were consistent at 4 weeks after injury and reperfusion. Conclusion: These findings confirm that the preischemic delivery of Hydrogen Sulfide limits ischemia-reperfusion injury–induced cellular damage in myotubes and skeletal muscle and suggests that, when given in the appropriate dose, this molecule may have significant therapeutic applications in multiple clinical scenarios. (Plast. Reconstr. Surg. 126: 1890, 2010.)
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Hydrogen Sulfide attenuates ischemia reperfusion injury in in vitro and in vivo models of intestine free tissue transfer
Plastic and Reconstructive Surgery, 2010Co-Authors: Peter W Henderson, Andrew L Weinstein, Josephine Sung, Sunil P Singh, Vijay Nagineni, Jason A SpectorAbstract:BACKGROUND: Ischemia-reperfusion injury is the propagation of injury following reintroduction of oxygen to previously ischemic tissue. The purpose of this study was to evaluate whether Hydrogen Sulfide provides protection against ischemia-reperfusion injury in enteric tissue. METHODS: In vitro (enterocyte anoxia-normoxia) and in vivo (rat intestinal ischemia-reperfusion) models of ischemia-reperfusion injury were tested with or without the addition of Hydrogen Sulfide. Apoptotic index was determined in vitro, and gross appearance, histology, and villus height (a measure of mucosal integrity) were assessed in vivo. Statistical analysis was performed, and significance was defined as p < 0.05. RESULTS: In vitro, cells treated with 10 microM Hydrogen Sulfide after 1-hour anoxia experienced a significant decrease in apoptotic index compared with untreated control (0.5 +/- 0.3 percent versus 2.8 +/- 0.7 percent); after 3 hours of anoxia, cells treated with 1 microM, 10 microM, and 100 microM Hydrogen Sulfide experienced significant decreases in apoptotic index versus untreated control (1.6 +/- 0.8 percent, 1.8 +/- 0.9 percent, and 2.8 +/- 0.7 percent versus 8.6 +/- 1.7 percent). In vivo, intestine treated with [10 microM] or [100 microM] Hydrogen Sulfide retained normal coloration and villus architecture after 1-hour ischemia; after 2 hours of ischemia, only intestine treated with [10 microM] Hydrogen Sulfide appeared uninjured. After 1, 2, or 3 hours of ischemia, villus heights of intestine treated with [10 microM] or [100 microM] Hydrogen Sulfide were significantly higher than heights of non-Hydrogen Sulfide-treated villi. CONCLUSIONS: Hydrogen Sulfide significantly attenuates ischemia-reperfusion injury in intestinal tissue in vitro and in vivo. These results have significant implications for enteric free tissue transfers and other gastrointestinal procedures in which ischemic intervals may be anticipated.
Ming Xian - One of the best experts on this subject based on the ideXlab platform.
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Hydrogen Sulfide h2s releasing agents chemistry and biological applications
ChemInform, 2014Co-Authors: Yu Zhao, Tyler D Biggs, Ming XianAbstract:Hydrogen Sulfide (H2S) is a newly recognized signaling molecule with very potent cytoprotective actions. The fields of H2S physiology and pharmacology have been rapidly growing in recent years, but a number of fundamental issues must be addressed to advance our understanding of the biology and clinical potential of H2S in the future. Hydrogen Sulfide releasing agents (also known as H2S donors) have been widely used in these fields. These compounds are not only useful research tools, but also potential therapeutic agents. It is therefore important to study the chemistry and pharmacology of exogenous H2S and to be aware of the limitations associated with the choice of donors used to generate H2S in vitro and in vivo. In this review we summarized the developments and limitations of currently available donors including H2S gas, Sulfide salts, garlic-derived sulfur compounds, Lawesson's reagent/analogs, 1,2-dithiole-3-thiones, thiol-activated donors, photo-caged donors, and thioamino acids. Some biological applications of these donors were also discussed.
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fluorescent probes based on nucleophilic substitution cyclization for Hydrogen Sulfide detection and bioimaging
Chemistry: A European Journal, 2014Co-Authors: Bo Peng, Chunrong Liu, Yu Zhao, Wei Chen, Ethan W Rosser, Armando Pacheco, Hector C Aguilar, Ming XianAbstract:The design, synthesis, properties, and cell imaging applications of a series of 2-pyridyl diSulfide based fluorescent probes (WSP1, WSP2, WSP3, WSP4 and WSP5) for Hydrogen Sulfide detection are reported. The strategy is based on the dual-nucleophilicity of Hydrogen Sulfide. A Hydrogen Sulfide mediated tandem nucleophilic substitution-cyclization reaction is used to release the fluorophores and turn on the fluorescence. The probes showed high sensitivity and selectivity for Hydrogen Sulfide over other reactive sulfur species, including cysteine and glutathione.
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Reaction based fluorescent probes for Hydrogen Sulfide
Organic Letters, 2012Co-Authors: Chunrong Liu, Chung Min Park, A. Richard Whorton, Sheng Li, Bo Peng, Ming XianAbstract:A reaction based fluorescence turn-on strategy for Hydrogen Sulfide (H2S) was developed. This strategy was based on a H2S-specific Michael addition?cyclization sequence. Other biological thiols such as cysteine and glutathione did not pursue the reaction and therefore did not turn on the fluorescence/consume the substrates. The probes showed good selectivity and sensitivity for Hydrogen Sulfide.
Peter W Henderson - One of the best experts on this subject based on the ideXlab platform.
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therapeutic delivery of Hydrogen Sulfide for salvage of ischemic skeletal muscle after the onset of critical ischemia
Journal of Vascular Surgery, 2011Co-Authors: Peter W Henderson, N Jimenez, J Ruffino, Allie M Sohn, Andrew L Weinstein, David D Krijgh, Alyssa J Reiffel, Jason A SpectorAbstract:Background Recent evidence suggests that Hydrogen Sulfide is capable of mitigating the degree of cellular damage associated with ischemia-reperfusion injury (IRI). Methods This study evaluated the potential utility of Hydrogen Sulfide in preventing IRI in skeletal muscle by using in vitro (cultured myotubes subjected to sequential hypoxia and normoxia) and in vivo (mouse hind limb ischemia, followed by reperfusion) models to determine whether intravenous Hydrogen Sulfide delivered after the ischemic event had occurred (pharmacologic postconditioning) conferred protection against IRI. Injury score and apoptotic index were determined by analysis of specimens stained with hematoxylin and eosin and terminal deoxynucleotide transferase-mediated deoxy-uridine triphosphate nick-end labeling, respectively. Results In vitro, Hydrogen Sulfide reduced the apoptotic index after 1, 3, or 5 hours of hypoxia by as much as 75% (P = .002), 80% (P = .006), and 83% (P Conclusions These findings confirm that Hydrogen Sulfide limits IRI-induced cellular damage in myotubes and skeletal muscle, even when delivered after the onset of ischemia in this murine model. These data suggest that when given in the appropriate dose and within the proper time frame, Hydrogen Sulfide may have significant therapeutic applications in multiple clinical scenarios.
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therapeutic metabolic inhibition Hydrogen Sulfide significantly mitigates skeletal muscle ischemia reperfusion injury in vitro and in vivo
Plastic and Reconstructive Surgery, 2010Co-Authors: Peter W Henderson, Andrew L Weinstein, David D Krijgh, Sunil P Singh, Vijay Nagineni, Daniel C Rafii, Daniel J Kadouch, Jason A SpectorAbstract:Background: Recent evidence suggests that Hydrogen Sulfide is capable of mitigating the degree of cellular damage associated with ischemia-reperfusion injury. The purpose of this study was to determine whether it is protective in skeletal muscle. Methods: This study used both in vitro (cultured myotubes subjected to sequential anoxia and normoxia) and in vivo (mouse hind-limb ischemia followed by reperfusion) models in which Hydrogen Sulfide (0 to 1000 M) was delivered before the onset of oxygen deficiency. Injury score and apoptotic index were determined by analysis of specimens stained with hematoxylin and eosin and terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling, respectively. Results: In vitro, Hydrogen Sulfide reduced the apoptotic index by as much as 99 percent (p 0.001), with optimal protection conferred by raising intravascular Hydrogen Sulfide to 10 M. In vivo, 10 M Hydrogen Sulfide delivered before 3 hours of hind-limb ischemia followed by 3 hours of reperfusion resulted in protection against ischemia-reperfusion injury–induced cellular changes, as evidenced by significant decreases in injury score and apoptotic index (by as much as 91 percent; p 0.001). These findings were consistent at 4 weeks after injury and reperfusion. Conclusion: These findings confirm that the preischemic delivery of Hydrogen Sulfide limits ischemia-reperfusion injury–induced cellular damage in myotubes and skeletal muscle and suggests that, when given in the appropriate dose, this molecule may have significant therapeutic applications in multiple clinical scenarios. (Plast. Reconstr. Surg. 126: 1890, 2010.)
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Hydrogen Sulfide attenuates ischemia reperfusion injury in in vitro and in vivo models of intestine free tissue transfer
Plastic and Reconstructive Surgery, 2010Co-Authors: Peter W Henderson, Andrew L Weinstein, Josephine Sung, Sunil P Singh, Vijay Nagineni, Jason A SpectorAbstract:BACKGROUND: Ischemia-reperfusion injury is the propagation of injury following reintroduction of oxygen to previously ischemic tissue. The purpose of this study was to evaluate whether Hydrogen Sulfide provides protection against ischemia-reperfusion injury in enteric tissue. METHODS: In vitro (enterocyte anoxia-normoxia) and in vivo (rat intestinal ischemia-reperfusion) models of ischemia-reperfusion injury were tested with or without the addition of Hydrogen Sulfide. Apoptotic index was determined in vitro, and gross appearance, histology, and villus height (a measure of mucosal integrity) were assessed in vivo. Statistical analysis was performed, and significance was defined as p < 0.05. RESULTS: In vitro, cells treated with 10 microM Hydrogen Sulfide after 1-hour anoxia experienced a significant decrease in apoptotic index compared with untreated control (0.5 +/- 0.3 percent versus 2.8 +/- 0.7 percent); after 3 hours of anoxia, cells treated with 1 microM, 10 microM, and 100 microM Hydrogen Sulfide experienced significant decreases in apoptotic index versus untreated control (1.6 +/- 0.8 percent, 1.8 +/- 0.9 percent, and 2.8 +/- 0.7 percent versus 8.6 +/- 1.7 percent). In vivo, intestine treated with [10 microM] or [100 microM] Hydrogen Sulfide retained normal coloration and villus architecture after 1-hour ischemia; after 2 hours of ischemia, only intestine treated with [10 microM] Hydrogen Sulfide appeared uninjured. After 1, 2, or 3 hours of ischemia, villus heights of intestine treated with [10 microM] or [100 microM] Hydrogen Sulfide were significantly higher than heights of non-Hydrogen Sulfide-treated villi. CONCLUSIONS: Hydrogen Sulfide significantly attenuates ischemia-reperfusion injury in intestinal tissue in vitro and in vivo. These results have significant implications for enteric free tissue transfers and other gastrointestinal procedures in which ischemic intervals may be anticipated.
Christopher J Chang - One of the best experts on this subject based on the ideXlab platform.
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azide based fluorescent probes imaging Hydrogen Sulfide in living systems
Methods in Enzymology, 2015Co-Authors: Vivian S Lin, Christopher J Chang, Alexander R LippertAbstract:Hydrogen Sulfide is a redox active sulfur species that is endogenously generated in mammalian systems as an antioxidant and signaling molecule to support cellular function. The fundamental and ubiquitous actions of Hydrogen Sulfide demand sensitive and specific methods to track this biomolecule as it is produced within living organisms with temporal and spatial regulation. In this context, the Hydrogen Sulfide-mediated reduction of an azide to an amine is a useful method for organic synthesis, and this reaction has successfully been exploited to yield biocompatible fluorescent probes for Hydrogen Sulfide detection in vitro and in cells. This chapter provides protocols and guidelines for applying azide-based fluorescence probes to detecting Hydrogen Sulfide in living systems, including a protocol that was used to detect endogenous Hydrogen Sulfide in living single cells using a confocal microscope.
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fluorescent probes for sensing and imaging biological Hydrogen Sulfide
Current Opinion in Chemical Biology, 2012Co-Authors: Vivian S Lin, Christopher J ChangAbstract:Hydrogen Sulfide (H2S) has long been recognized as a toxic molecule in biological systems. However, emerging studies now link controlled fluxes of this reactive sulfur species to cellular regulation and signaling events akin to other small molecule messengers, such as nitric oxide, Hydrogen peroxide, and carbon monoxide. Progress in the development of fluorescent small-molecule indicators with high selectivity for Hydrogen Sulfide offers a promising approach for studying its production, trafficking, and downstream physiological and/or pathological effects.
Bo Peng - One of the best experts on this subject based on the ideXlab platform.
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fluorescent probes based on nucleophilic substitution cyclization for Hydrogen Sulfide detection and bioimaging
Chemistry: A European Journal, 2014Co-Authors: Bo Peng, Chunrong Liu, Yu Zhao, Wei Chen, Ethan W Rosser, Armando Pacheco, Hector C Aguilar, Ming XianAbstract:The design, synthesis, properties, and cell imaging applications of a series of 2-pyridyl diSulfide based fluorescent probes (WSP1, WSP2, WSP3, WSP4 and WSP5) for Hydrogen Sulfide detection are reported. The strategy is based on the dual-nucleophilicity of Hydrogen Sulfide. A Hydrogen Sulfide mediated tandem nucleophilic substitution-cyclization reaction is used to release the fluorophores and turn on the fluorescence. The probes showed high sensitivity and selectivity for Hydrogen Sulfide over other reactive sulfur species, including cysteine and glutathione.
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Reaction based fluorescent probes for Hydrogen Sulfide
Organic Letters, 2012Co-Authors: Chunrong Liu, Chung Min Park, A. Richard Whorton, Sheng Li, Bo Peng, Ming XianAbstract:A reaction based fluorescence turn-on strategy for Hydrogen Sulfide (H2S) was developed. This strategy was based on a H2S-specific Michael addition?cyclization sequence. Other biological thiols such as cysteine and glutathione did not pursue the reaction and therefore did not turn on the fluorescence/consume the substrates. The probes showed good selectivity and sensitivity for Hydrogen Sulfide.
