The Experts below are selected from a list of 14109 Experts worldwide ranked by ideXlab platform
Yoichiro Ito - One of the best experts on this subject based on the ideXlab platform.
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:Abstract An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches,
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches, <0.05 - 0.84%, and suggested that BTZ contamination could be reduced by appropriate adjustments in the manufacturing process. An explanation of the likely source of BTZ - as a side-reaction product in a particular step of the manufacturing process - was also presented.
Adrian Weisz - One of the best experts on this subject based on the ideXlab platform.
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:Abstract An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches,
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches, <0.05 - 0.84%, and suggested that BTZ contamination could be reduced by appropriate adjustments in the manufacturing process. An explanation of the likely source of BTZ - as a side-reaction product in a particular step of the manufacturing process - was also presented.
Marianita Perezgonzalez - One of the best experts on this subject based on the ideXlab platform.
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:Abstract An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches,
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches, <0.05 - 0.84%, and suggested that BTZ contamination could be reduced by appropriate adjustments in the manufacturing process. An explanation of the likely source of BTZ - as a side-reaction product in a particular step of the manufacturing process - was also presented.
Jackson F Wood - One of the best experts on this subject based on the ideXlab platform.
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:Abstract An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches,
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches, <0.05 - 0.84%, and suggested that BTZ contamination could be reduced by appropriate adjustments in the manufacturing process. An explanation of the likely source of BTZ - as a side-reaction product in a particular step of the manufacturing process - was also presented.
Clark D Ridge - One of the best experts on this subject based on the ideXlab platform.
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:Abstract An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches,
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identification separation by spiral high speed counter current chromatography and quantification of 7 chloro 5 methyl 2h 1 4 benzothiazin 3 4h one an impurity in the thioindigoid color additive d c red no 30 and its lakes
Journal of Chromatography A, 2021Co-Authors: Adrian Weisz, Marianita Perezgonzalez, Jackson F Wood, Clark D Ridge, Yoichiro ItoAbstract:An impurity in the color additives D&C Red No. 30 (R30) and D&C Red No. 30 lakes (R30L) was newly identified and characterized as 7-chloro-5-methyl-2H-1,4-benzothiazin-3(4H)-one (BTZ), and its extent and level in certified batches of these color additives was determined. BTZ was extracted from the dye with ethanol, resulting in a crude extract enriched to a concentration of over 60%. BTZ was then separated from a portion of the enriched extract by high-speed counter-current chromatography using a spiral-tube assembly column with intermittently pressed tubing of 60 ml capacity. It was the first reported use of such a column to separate a small, moderately Hydrophobic Compound. The two-phase solvent system was also moderately Hydrophobic, consisting of hexane-ethyl acetate-methanol-water (5:2:5:2), and the retention of the organic stationary phase measured after the separation was 83.3%. The separation yielded BTZ of two purity grades, the higher of which (~95.5%) was used as a standard to quantify the impurity in 37 batches of R30 and R30L using an HPLC method developed and validated for that purpose. Analyses revealed a wide range of BTZ levels across batches, <0.05 - 0.84%, and suggested that BTZ contamination could be reduced by appropriate adjustments in the manufacturing process. An explanation of the likely source of BTZ - as a side-reaction product in a particular step of the manufacturing process - was also presented.
