The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Grace Q. Chen - One of the best experts on this subject based on the ideXlab platform.
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Current progress towards the metabolic engineering of plant seed oil for Hydroxy Fatty Acids production
Plant Cell Reports, 2015Co-Authors: Grace Q. ChenAbstract:Key message Hydroxy Fatty Acids produced in plant seed oil are important industrial material. This review focuses on the use of metabolic engineering approaches for the production of Hydroxy Fatty Acids in transgenic plants. Abstract Vegetable oil is not only edible but can also be used for industrial purposes. The industrial demand for vegetable oil will increase with the continued depletion of fossil fuels and ensuing environmental issues such as climate change, caused by increased carbon dioxide in the air. Some plants accumulate high levels of unusual Fatty Acids in their seeds, and these Fatty Acids (FAs) have properties that make them suitable for industrial applications. Hydroxy Fatty Acids (HFAs) are some of the most important of these industrial FAs. Castor oil is the conventional source of HFA. However, due to the presence of toxin ricin in its seeds, castor is not cultivated on a large scale. Lesquerella is another HFA accumulator and is currently being developed as a new crop for a safe source of HFAs. The mechanisms of HFA synthesis and accumulation have been extensively studied using castor genes and the model plant Arabidopsis . HFAs accumulated to 17 % in the seed oil of Arabidopsis expressing a FA Hydroxylase gene from castor ( RcFAH12 ), but its seed oil content and plant growth decreased. When RcFAH12 gene was coexpressed with additional castor gene(s) in Arabidopsis , ~30 % HFAs were accumulated and the seed oil content and plant growth was almost restored to the wild-type level. Further advancement of our understanding of pathways, genes and regulatory mechanisms underlying synthesis and accumulation of HFAs is essential to developing and implementing effective genetic approaches for enhancing HFA production in oilseeds.
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current progress towards the metabolic engineering of plant seed oil for Hydroxy Fatty Acids production
Plant Cell Reports, 2015Co-Authors: Grace Q. ChenAbstract:Key message Hydroxy Fatty Acids produced in plant seed oil are important industrial material. This review focuses on the use of metabolic engineering approaches for the production of Hydroxy Fatty Acids in transgenic plants.
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Current progress towards the metabolic engineering of plant seed oil for Hydroxy Fatty Acids production.
Plant cell reports, 2015Co-Authors: Kyeong-ryeol Lee, Grace Q. Chen, Hyun Uk KimAbstract:KEY MESSAGE: Hydroxy Fatty Acids produced in plant seed oil are important industrial material. This review focuses on the use of metabolic engineering approaches for the production of Hydroxy Fatty Acids in transgenic plants. Vegetable oil is not only edible but can also be used for industrial purposes. The industrial demand for vegetable oil will increase with the continued depletion of fossil fuels and ensuing environmental issues such as climate change, caused by increased carbon dioxide in the air. Some plants accumulate high levels of unusual Fatty Acids in their seeds, and these Fatty Acids (FAs) have properties that make them suitable for industrial applications. Hydroxy Fatty Acids (HFAs) are some of the most important of these industrial FAs. Castor oil is the conventional source of HFA. However, due to the presence of toxin ricin in its seeds, castor is not cultivated on a large scale. Lesquerella is another HFA accumulator and is currently being developed as a new crop for a safe source of HFAs. The mechanisms of HFA synthesis and accumulation have been extensively studied using castor genes and the model plant Arabidopsis. HFAs accumulated to 17% in the seed oil of Arabidopsis expressing a FA Hydroxylase gene from castor (RcFAH12), but its seed oil content and plant growth decreased. When RcFAH12 gene was coexpressed with additional castor gene(s) in Arabidopsis, ~30% HFAs were accumulated and the seed oil content and plant growth was almost restored to the wild-type level. Further advancement of our understanding of pathways, genes and regulatory mechanisms underlying synthesis and accumulation of HFAs is essential to developing and implementing effective genetic approaches for enhancing HFA production in oilseeds.
Ljerka Kunst - One of the best experts on this subject based on the ideXlab platform.
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Production of Hydroxy Fatty Acids in Arabidopsis Thaliana
Advanced Research on Plant Lipids, 2020Co-Authors: Mark Andrew Smith, Gangamma Chowrira, Ljerka KunstAbstract:Castor bean (Ricinus communis) produces a seed oil that contains nearly 90% ricinoleic acid (12-Hydroxyoctadeca-9-cis-enoic acid: 18:1-OH). This Fatty acid is a valuable industrial raw material and is used in a wide variety of processes and products. In castor, 18:1-OH is synthesized by the 012-Hydroxylation of oleate esterified to the sn-2 position of phosphatidylcholine (Bafor et al., 1991). The reaction is catalysed by an enzyme that is closely related to a FAD2 (ER-Δ12) desaturase (van de Loo et al., 1995). We have been using Arabidopsis lines, transformed with a gene construct encoding the castor Hydroxylase, as a model system to study the synthesis and accumulation of Hydroxy Fatty Acids in the seed.
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a condensing enzyme from the seeds of lesquerella fendleri that specifically elongates Hydroxy Fatty Acids
Plant Physiology, 2001Co-Authors: Hangsik Moon, Mark Andrew Smith, Ljerka KunstAbstract:Lesquerella fendleri seed oil contains up to 60% Hydroxy Fatty Acids, nearly all of which is the 20-carbon Hydroxy Fatty acid lesquerolic acid (d-14-Hydroxyeicos-cis-11-enoic acid). Previous work suggested that lesquerolic acid in L . fendleri was formed by the elongation of the 18-carbon Hydroxy Fatty acid, ricinoleic acid. To identify a gene encoding the enzyme involved in Hydroxy Fatty acid elongation, an L . fendleri genomic DNA library was screened using the coding region of the Arabidopsis Fatty Acid Elongation1 gene as a probe. A gene, LfKCS3 , with a high sequence similarity to known very long-chain Fatty acid condensing enzymes, was isolated. LfKCS3 has a 2,062-bp open reading frame interrupted by two introns, which encodes a polypeptide of 496 amino Acids. LfKCS3 transcripts accumulated only in the embryos of L . fendleri and first appeared in the early stages of development. Fusion of the LfKCS3 promoter to the uidA reporter gene and expression in transgenic Arabidopsis resulted in a high level of β-glucuronidase activity exclusively in developing embryos. Seeds of Arabidopsis plants transformed with LfKCS3 showed no change in their very long-chain Fatty acid content. However, when these Arabidopsis plants were crossed with the transgenic plants expressing the castor oleate 12-Hydroxylase, significant amounts of 20-carbon Hydroxy Fatty Acids accumulated in the seed, indicating that the LfKCS3 condensing enzyme specifically catalyzes elongation of 18-carbon Hydroxy Fatty Acids.
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Production of Hydroxy Fatty Acids in the seeds of Arabidopsis thaliana.
Biochemical Society Transactions, 2000Co-Authors: Mark Andrew Smith, Hangsik Moon, Ljerka KunstAbstract:Seed-specific expression in Arabidopsis thaliana of oleate Hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of Hydroxy Fatty Acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n - 3 desaturase (FAD3) and the FAEl condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain Hydroxy Fatty Acids, respectively. In Arabidopsis plants with an active ER A1 2-oleate desaturase the presence of Hydroxy Fatty Acids corresponded to an increase in the levels of 18 : 1 and a decrease in 18 : 2 levels. Expression in yeast indicates that the castor Hydroxylase also has a low level of desaturase activity.
Zeper Abliz - One of the best experts on this subject based on the ideXlab platform.
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Strategy for Global Profiling and Identification of 2- and 3-Hydroxy Fatty Acids in Plasma by UPLC-MS/MS.
Analytical Chemistry, 2020Co-Authors: Jiangshuo Li, Jing Xu, Ruiping Zhang, Jiuming He, Yanhua Chen, Guanggen Jiao, Zeper AblizAbstract:2-Hydroxy Fatty Acids (2-OHFAs) and 3-Hydroxy Fatty Acids (3-OHFAs) with the same carbon backbone are isomers, both of which are closely related to diseases involving Fatty acid oxidation disorder....
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strategy for global profiling and identification of 2 and 3 Hydroxy Fatty Acids in plasma by uplc ms ms
Analytical Chemistry, 2020Co-Authors: Jiangshuo Li, Jing Xu, Ruiping Zhang, Jiuming He, Yanhua Chen, Guanggen Jiao, Zeper AblizAbstract:2-Hydroxy Fatty Acids (2-OHFAs) and 3-Hydroxy Fatty Acids (3-OHFAs) with the same carbon backbone are isomers, both of which are closely related to diseases involving Fatty acid oxidation disorder....
Hangsik Moon - One of the best experts on this subject based on the ideXlab platform.
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a condensing enzyme from the seeds of lesquerella fendleri that specifically elongates Hydroxy Fatty Acids
Plant Physiology, 2001Co-Authors: Hangsik Moon, Mark Andrew Smith, Ljerka KunstAbstract:Lesquerella fendleri seed oil contains up to 60% Hydroxy Fatty Acids, nearly all of which is the 20-carbon Hydroxy Fatty acid lesquerolic acid (d-14-Hydroxyeicos-cis-11-enoic acid). Previous work suggested that lesquerolic acid in L . fendleri was formed by the elongation of the 18-carbon Hydroxy Fatty acid, ricinoleic acid. To identify a gene encoding the enzyme involved in Hydroxy Fatty acid elongation, an L . fendleri genomic DNA library was screened using the coding region of the Arabidopsis Fatty Acid Elongation1 gene as a probe. A gene, LfKCS3 , with a high sequence similarity to known very long-chain Fatty acid condensing enzymes, was isolated. LfKCS3 has a 2,062-bp open reading frame interrupted by two introns, which encodes a polypeptide of 496 amino Acids. LfKCS3 transcripts accumulated only in the embryos of L . fendleri and first appeared in the early stages of development. Fusion of the LfKCS3 promoter to the uidA reporter gene and expression in transgenic Arabidopsis resulted in a high level of β-glucuronidase activity exclusively in developing embryos. Seeds of Arabidopsis plants transformed with LfKCS3 showed no change in their very long-chain Fatty acid content. However, when these Arabidopsis plants were crossed with the transgenic plants expressing the castor oleate 12-Hydroxylase, significant amounts of 20-carbon Hydroxy Fatty Acids accumulated in the seed, indicating that the LfKCS3 condensing enzyme specifically catalyzes elongation of 18-carbon Hydroxy Fatty Acids.
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Production of Hydroxy Fatty Acids in the seeds of Arabidopsis thaliana.
Biochemical Society Transactions, 2000Co-Authors: Mark Andrew Smith, Hangsik Moon, Ljerka KunstAbstract:Seed-specific expression in Arabidopsis thaliana of oleate Hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of Hydroxy Fatty Acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n - 3 desaturase (FAD3) and the FAEl condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain Hydroxy Fatty Acids, respectively. In Arabidopsis plants with an active ER A1 2-oleate desaturase the presence of Hydroxy Fatty Acids corresponded to an increase in the levels of 18 : 1 and a decrease in 18 : 2 levels. Expression in yeast indicates that the castor Hydroxylase also has a low level of desaturase activity.
Mark Andrew Smith - One of the best experts on this subject based on the ideXlab platform.
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Production of Hydroxy Fatty Acids in Arabidopsis Thaliana
Advanced Research on Plant Lipids, 2020Co-Authors: Mark Andrew Smith, Gangamma Chowrira, Ljerka KunstAbstract:Castor bean (Ricinus communis) produces a seed oil that contains nearly 90% ricinoleic acid (12-Hydroxyoctadeca-9-cis-enoic acid: 18:1-OH). This Fatty acid is a valuable industrial raw material and is used in a wide variety of processes and products. In castor, 18:1-OH is synthesized by the 012-Hydroxylation of oleate esterified to the sn-2 position of phosphatidylcholine (Bafor et al., 1991). The reaction is catalysed by an enzyme that is closely related to a FAD2 (ER-Δ12) desaturase (van de Loo et al., 1995). We have been using Arabidopsis lines, transformed with a gene construct encoding the castor Hydroxylase, as a model system to study the synthesis and accumulation of Hydroxy Fatty Acids in the seed.
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a condensing enzyme from the seeds of lesquerella fendleri that specifically elongates Hydroxy Fatty Acids
Plant Physiology, 2001Co-Authors: Hangsik Moon, Mark Andrew Smith, Ljerka KunstAbstract:Lesquerella fendleri seed oil contains up to 60% Hydroxy Fatty Acids, nearly all of which is the 20-carbon Hydroxy Fatty acid lesquerolic acid (d-14-Hydroxyeicos-cis-11-enoic acid). Previous work suggested that lesquerolic acid in L . fendleri was formed by the elongation of the 18-carbon Hydroxy Fatty acid, ricinoleic acid. To identify a gene encoding the enzyme involved in Hydroxy Fatty acid elongation, an L . fendleri genomic DNA library was screened using the coding region of the Arabidopsis Fatty Acid Elongation1 gene as a probe. A gene, LfKCS3 , with a high sequence similarity to known very long-chain Fatty acid condensing enzymes, was isolated. LfKCS3 has a 2,062-bp open reading frame interrupted by two introns, which encodes a polypeptide of 496 amino Acids. LfKCS3 transcripts accumulated only in the embryos of L . fendleri and first appeared in the early stages of development. Fusion of the LfKCS3 promoter to the uidA reporter gene and expression in transgenic Arabidopsis resulted in a high level of β-glucuronidase activity exclusively in developing embryos. Seeds of Arabidopsis plants transformed with LfKCS3 showed no change in their very long-chain Fatty acid content. However, when these Arabidopsis plants were crossed with the transgenic plants expressing the castor oleate 12-Hydroxylase, significant amounts of 20-carbon Hydroxy Fatty Acids accumulated in the seed, indicating that the LfKCS3 condensing enzyme specifically catalyzes elongation of 18-carbon Hydroxy Fatty Acids.
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Production of Hydroxy Fatty Acids in the seeds of Arabidopsis thaliana.
Biochemical Society Transactions, 2000Co-Authors: Mark Andrew Smith, Hangsik Moon, Ljerka KunstAbstract:Seed-specific expression in Arabidopsis thaliana of oleate Hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of Hydroxy Fatty Acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n - 3 desaturase (FAD3) and the FAEl condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain Hydroxy Fatty Acids, respectively. In Arabidopsis plants with an active ER A1 2-oleate desaturase the presence of Hydroxy Fatty Acids corresponded to an increase in the levels of 18 : 1 and a decrease in 18 : 2 levels. Expression in yeast indicates that the castor Hydroxylase also has a low level of desaturase activity.
