The Experts below are selected from a list of 1296 Experts worldwide ranked by ideXlab platform
Yanmei Wang - One of the best experts on this subject based on the ideXlab platform.
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synthesis of Hydroxyethylcellulose g methoxypoly ethylene glycol copolymer and its application for protein separation in ce
Journal of Applied Polymer Science, 2012Co-Authors: Xiaohua Shi, Lin Tan, Jinxing Xing, Fuhu Cao, Lijuan Chen, Zhaofeng Luo, Yanmei WangAbstract:Hydroxyethylcellulose-g-methoxypoly (ethylene glycol) (HEC-g-PEG) graft copolymers were synthesized through the etherification reaction between the hydroxyl group of Hydroxyethylcellulose (HEC) and iodinated methoxypoly (ethylene glycol) (PEG-I), which was prepared on the basis of two-step reaction. Fourier transforms infrared spectrum (FTIR), nuclear magnetic resonance (NMR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and iodide oxidation method were used to prove the success of synthesis of graft copolymer. Furthermore, the comparative studies of electro-osmotic flow (EOF) and protein separation in bare-fused silica, HEC and HEC-g-PEG-coated capillary were performed in capillary electrophoresis (CE). The results showed that HEC-g-PEG-coated capillary presented efficient EOF suppression ability and excellent resisting protein adsorption ability. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
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Hydroxyethylcellulose graft poly 4 vinylpyridine as a novel adsorbed coating for protein separation by ce
Electrophoresis, 2009Co-Authors: Runmiao Yang, Yuhai Liu, Yanmei WangAbstract:A novel graft copolymer, Hydroxyethylcellulose-graft-poly (4-vinylpyridine), used as a physically adsorbed coating of the fused-silica (FS) capillaries, was synthesized by using ceric ammonium nitrate initiator in aqueous nitric acid solution. EOF measurements showed that the above graft copolymer coating of the FS capillaries could suppress EOF effectively compared with the bare FS capillaries. The adsorbed coating exhibited minimal interactions with proteins, providing efficient protein separations with excellent repeatability. Interestingly, the electrical charge of the coated capillary wall could be modulated by varying the pH of the running buffer, which makes possible the analysis of basic and acidic proteins in the same capillary. The effects of pH on suppressing EOF and protein separation were investigated in detail. The results demonstrated that the Hydroxyethylcellulose-graft-poly (4-vinylpyridine) copolymer coatings have great potential in the diagnosis and proteomics.
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Hydroxyethylcellulose graft poly n n dimethylacrylamide copolymer as a multifunctional separation medium for ce
Electrophoresis, 2008Co-Authors: Shuhua Peng, Ronghua Shi, Runmiao Yang, Dan Zhou, Yanmei WangAbstract:A new multifunctional separation medium, Hydroxyethylcellulose-graft-poly (N,N-dimethylacrylamide) copolymer synthesized in our laboratory for application in both basic protein separation and dsDNA separation by CE, is presented in this paper. As a noncovalent coating, this medium showed a powerful capability in resisting basic protein adsorption. Highly efficient and rapid protein separation had been obtained at four different pH values. Meanwhile, the 11 fragments of the dsDNA sample could be baseline separated using this grafted copolymer as sieving matrix at an appropriate concentration.
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cationized Hydroxyethylcellulose as a novel adsorbed coating for basic protein separation by capillary electrophoresis
Electrophoresis, 2008Co-Authors: Runmiao Yang, Ronghua Shi, Shuhua Peng, Dan Zhou, Hang Liu, Yanmei WangAbstract:We present cationized Hydroxyethylcellulose (cat-HEC) synthesized in our laboratory as a novel physically adsorbed coating for CE. This capillary coating is simple and easy to obtain as it only requires flushing the capillary with polymer aqueous solution. A comparative study with and without polymers was performed. The adsorbed cat-HEC coating exhibited minimal interactions with basic proteins, providing efficient basic protein separations with excellent reproducibility. Under broad pHs, the amine groups are the main charged groups bringing about a global positive charge on the capillary wall. As a consequence, the cat-HEC coating produced an anodal EOF performance. A comparative study on the use of Hydroxyethylcellulose (HEC) and cat-HEC as physically adsorbed coatings for CE are also presented. The separation efficiency and analysis reproducibility proved that the cat-HEC polymer was efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. The long-term stability of the cat-HEC coating in consecutive protein separation runs has demonstrated the suitability of the coating for high-throughput electrophoretic protein separations.
Vitaliy V Khutoryanskiy - One of the best experts on this subject based on the ideXlab platform.
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structure and characterisation of Hydroxyethylcellulose silica nanoparticles
RSC Advances, 2018Co-Authors: Edward D H Mansfield, Yash Pandya, Ellina A Mun, Sarah E Rogers, Inbal Abutbulionita, Dganit Danino, Adrian C Williams, Vitaliy V KhutoryanskiyAbstract:Functionalising nanoparticles with polymers has gained much interest in recent years, as it aids colloidal stability and manipulation of surface properties. Here, polymer-coated thiolated silica nanoparticles were synthesised by self-condensation of 3-mercaptopropyltrimethoxysilane in the presence of Hydroxyethylcellulose. These nanoparticles were characterised by dynamic light scattering, small angle neutron scattering, Nanoparticle Tracking Analysis, Raman spectroscopy, FT-IR spectroscopy, thermogravimetric analysis, Ellman's assay, transmission electron microscopy and cryo-transmission electron microscopy. It was found that increasing the amount of Hydroxyethylcellulose in the reaction mixture increased the nanoparticle size and reduced the number of thiol groups on their surface. Additionally, by utilising small angle neutron scattering and dynamic light scattering, it was demonstrated that higher concentrations of polymer in the reaction mixture (0.5–2% w/v) resulted in the formation of aggregates, whereby several silica nanoparticles are bridged together with macromolecules of Hydroxyethylcellulose. A correlation was identified between the aggregate size and number of particles per aggregate based on size discrepancies observed between DLS and SANS measurements. This information makes it possible to control the size of aggregates during a simple one-pot synthesis; a prospect highly desirable in the design of potential drug delivery systems.
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On the Role of Specific Interactions in the Diffusion of Nanoparticles in Aqueous Polymer Solutions
2016Co-Authors: Ellina A Mun, Sarah E Rogers, Adrian C Williams, Claire Hannell, Patrick Hole, Vitaliy V KhutoryanskiyAbstract:ABSTRACT: Understanding nanoparticle diffusion within non-Newtonian biological and synthetic fluids is essential in designing novel formulations (e.g., nanomedicines for drug delivery, shampoos, lotions, coatings, paints, etc.), but is presently poorly defined. This study reports the diffusion of thiolated and PEGylated silica nanoparticles, characterized by small-angle neutron scattering, in solutions of various water-soluble polymers such as poly(acrylic acid) (PAA), poly(N-vinylpyrrolidone) (PVP), poly(ethylene oxide) (PEO), and Hydroxyethylcellulose (HEC) probed using NanoSight nano-particle tracking analysis. Results show that the diffusivity of nanoparticles is affected by their dimensions, medium viscosity, and, in particular, the specific interactions between nanoparticles and the macromolecules in solution; strong attractive interactions such as hydrogen bonding hampe
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mucoadhesive and elastic films based on blends of chitosan and Hydroxyethylcellulose
Macromolecular Bioscience, 2008Co-Authors: Kun Luo, Jingbo Yin, Olga V Khutoryanskaya, Vitaliy V KhutoryanskiyAbstract:Mucoadhesive polymeric films have been prepared based on blends of chitosan and Hydroxyethylcellulose. The blends have been characterized by IR spectroscopy, DSC, WAXD, TGA, SEM, and mechanical testing. It is demonstrated that the mechanical properties of chitosan are improved significantly upon blending with Hydroxyethylcellulose. An increase in Hydroxyethylcellulose content in the blends makes the materials more elastic. The thermal treatment of the blends at 100 degrees C leads to partial cross-linking of the polymers and formation of water-insoluble but swellable materials. The adhesion of the films towards porcine buccal mucosa decreases with increasing Hydroxyethylcellulose content in the blends.
Runmiao Yang - One of the best experts on this subject based on the ideXlab platform.
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Hydroxyethylcellulose graft poly 4 vinylpyridine as a novel adsorbed coating for protein separation by ce
Electrophoresis, 2009Co-Authors: Runmiao Yang, Yuhai Liu, Yanmei WangAbstract:A novel graft copolymer, Hydroxyethylcellulose-graft-poly (4-vinylpyridine), used as a physically adsorbed coating of the fused-silica (FS) capillaries, was synthesized by using ceric ammonium nitrate initiator in aqueous nitric acid solution. EOF measurements showed that the above graft copolymer coating of the FS capillaries could suppress EOF effectively compared with the bare FS capillaries. The adsorbed coating exhibited minimal interactions with proteins, providing efficient protein separations with excellent repeatability. Interestingly, the electrical charge of the coated capillary wall could be modulated by varying the pH of the running buffer, which makes possible the analysis of basic and acidic proteins in the same capillary. The effects of pH on suppressing EOF and protein separation were investigated in detail. The results demonstrated that the Hydroxyethylcellulose-graft-poly (4-vinylpyridine) copolymer coatings have great potential in the diagnosis and proteomics.
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Hydroxyethylcellulose graft poly n n dimethylacrylamide copolymer as a multifunctional separation medium for ce
Electrophoresis, 2008Co-Authors: Shuhua Peng, Ronghua Shi, Runmiao Yang, Dan Zhou, Yanmei WangAbstract:A new multifunctional separation medium, Hydroxyethylcellulose-graft-poly (N,N-dimethylacrylamide) copolymer synthesized in our laboratory for application in both basic protein separation and dsDNA separation by CE, is presented in this paper. As a noncovalent coating, this medium showed a powerful capability in resisting basic protein adsorption. Highly efficient and rapid protein separation had been obtained at four different pH values. Meanwhile, the 11 fragments of the dsDNA sample could be baseline separated using this grafted copolymer as sieving matrix at an appropriate concentration.
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cationized Hydroxyethylcellulose as a novel adsorbed coating for basic protein separation by capillary electrophoresis
Electrophoresis, 2008Co-Authors: Runmiao Yang, Ronghua Shi, Shuhua Peng, Dan Zhou, Hang Liu, Yanmei WangAbstract:We present cationized Hydroxyethylcellulose (cat-HEC) synthesized in our laboratory as a novel physically adsorbed coating for CE. This capillary coating is simple and easy to obtain as it only requires flushing the capillary with polymer aqueous solution. A comparative study with and without polymers was performed. The adsorbed cat-HEC coating exhibited minimal interactions with basic proteins, providing efficient basic protein separations with excellent reproducibility. Under broad pHs, the amine groups are the main charged groups bringing about a global positive charge on the capillary wall. As a consequence, the cat-HEC coating produced an anodal EOF performance. A comparative study on the use of Hydroxyethylcellulose (HEC) and cat-HEC as physically adsorbed coatings for CE are also presented. The separation efficiency and analysis reproducibility proved that the cat-HEC polymer was efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. The long-term stability of the cat-HEC coating in consecutive protein separation runs has demonstrated the suitability of the coating for high-throughput electrophoretic protein separations.
Libero Italo Giannola - One of the best experts on this subject based on the ideXlab platform.
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effects of gamma irradiation on trehalose Hydroxyethylcellulose microspheres loaded with vancomycin
European Journal of Pharmaceutics and Biopharmaceutics, 2005Co-Authors: A Bartolotta, Giulia Giandalia, Viviana De Caro, Libero Italo Giannola, M C Doca, Monica Campisi, M Brai, E CalderaroAbstract:Ionizing radiation can be used as a drug sterilization technique, provided that the drug itself is not modified and that no toxic products are produced; moreover, if the irradiated product is a drug delivery system, the drug release characteristics must not be significantly altered by radiation. The aim of this work was to study the effects of sterilization by ionizing radiation on Hydroxyethylcellulose/trehalose spherical micromatrices, containing the antibiotic vancomycin. Our experimental results showed that gamma-rays did not alter the chromophore groups of vancomycin (UV measurements), and did not modify the kinetic behavior of drug release from microspheres. Moreover, no significant changes in the shape and in the size distribution of microspheres were found after irradiation. The electron spin resonance (ESR) spectroscopy was proven to be a valid identification method of the executed radiation treatment, even after 5 years. The experimental results showed that the therapeutic application of the pharmacological system investigated was not compromised by irradiation, and that ESR spectroscopy can be used to distinguish irradiated from non-irradiated products.
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trehalose Hydroxyethylcellulose microspheres containing vancomycin for topical drug delivery
European Journal of Pharmaceutics and Biopharmaceutics, 2001Co-Authors: Giulia Giandalia, Viviana De Caro, Lorenzo Cordone, Libero Italo GiannolaAbstract:Abstract A new formulation, in which vancomycin is entrapped into trehalose and Hydroxyethylcellulose (Natrosol ® ) spherical matrices, is described. Microspheres were produced by the solvent evaporation method. The entrapped drug was fully recovered following microspheres dissolution. Differential scanning calorimetry analyses proved that Natrosol maintains trehalose in its amorphous form. The stabilizing effects of trehalose on vancomycin were evaluated even after long storage and heating of microspheres. Calorimetric data indicated no decomposition of the entrapped drug. In vitro drug release, already performed by using a general two-compartment linear time-invariant open model, suggests that the new delivery system is suitable for topical application on extensive and purulent or burn wounds, when the skin is heavily damaged and the barrier disrupted. The system activation is determined by osmotic phenomena. The prepared new delivery system seems to have characteristics suitable for topical applications on extensive and purulent wounds. The system is able to take away serous exudates from wounds, thus letting the matrix to swell and form a viscous gel-like dispersion that, in turn, enables drug diffusion.
Robert Y M Huang - One of the best experts on this subject based on the ideXlab platform.
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pervaporation dehydration of ethanol water mixtures with chitosan Hydroxyethylcellulose cs hec composite membranes i effect of operating conditions
Journal of Membrane Science, 2002Co-Authors: Ratana Jiraratananon, Ampai Chanachai, Robert Y M Huang, Dudsadee UttapapAbstract:Abstract Composite hydrophilic pervaporation membranes were prepared from chitosan blended with Hydroxyethylcellulose using cellulose acetate as a porous support. The membranes were tested for dehydration performance of ethanol–water mixtures of ethanol concentrations 70–95 wt.% in the laminar flow region, at temperatures 50–70°C and at permeate pressures of 3–30 mmHg. The composite membrane showed an improved dehydration performance compared with dense CS/HEC membrane developed earlier. The effects of operating conditions also revealed that pervaporation of low water content feed carried out at high feed flow rate and at low temperature and permeate pressure was an advantage.
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pervaporation with chitosan Hydroxyethylcellulose cs hec blended membranes
Journal of Membrane Science, 2000Co-Authors: Ampai Chanachai, Ratana Jiraratananon, Dudsadee Uttapap, Go Young Moon, William A Anderson, Robert Y M HuangAbstract:Abstract Chitosan/Hydroxyethylcellulose (CS/HEC) blended membranes were prepared and crosslinked with a urea–formaldehyde–sulfuric acid mixture. The chemically crosslinking structure of blended membrane was analyzed by IR spectroscopy. The CS/HEC-3/1 and CS/HEC-9/1 blended membranes are appropriate for the dehydration of ethanol–water and isopropanol–water mixtures, respectively. These membranes have high selectivity and promising flux, especially at high alcohol concentration (e.g., J = 112 g m −2 h −1 , separation factor ( α w/e ) = 10,491 for 90 wt.% ethanol solution and J = 175 g m −2 h −1 , α w/i = 26091 for 90 wt.% isopropanol solution). Flux increased while the separation factor decreased with increasing water content in the feed. The pervaporation (PV) behavior of the isopropanol–water mixture is similar to that of the ethanol–water mixture. The effect of HEC on the sorption is an important factor for changes in the pervaporation performance of CS/HEC membranes.
