The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Debi P. Sarkar - One of the best experts on this subject based on the ideXlab platform.
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Targeted delivery of <B>HygromycinB> B using reconstituted Sendai viral envelopes lacking hemagglutinin‐neuraminidase
FEBS letters, 1993Co-Authors: Sangeeta Bagai, Debi P. SarkarAbstract:<B>HygromycinB> B was encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes). IncuBation of loaded F-virosomes with cultured HepG2 cells resulted in fusion mediated delivery of <B>HygromycinB> B to the cell cytoplasm, as was inferred from inhiBition of DNA synthesis. Binding of the F-virosomes to HepG2 cells was mediated By the interaction of terminal β-galactose residues of fusion protein with asialoglycoprotein receptor on HepG2 cells, suBsequently leading to fusion Between the two memBranes. The cytotoxic effect of <B>HygromycinB> B enclosed in F-virosomes was comparaBle with that of F,HN-virosomes containing Both hemagglutinin-neuraminidase (HN) and F protein and F,HNred-virosomes containing HN whose disulfide Bonds were irreversiBly reduced (HNred). <B>HygromycinB> B loaded fusogenic liposomes were prepared By coreconstituting the viral envelope containing only fusion protein with exogenous lipids. These fusogenic liposomes were found to Be more active than F-virosomes at the same fusion protein concentrations.
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targeted delivery of <B>HygromycinB> B using reconstituted sendai viral envelopes lacking hemagglutinin neuraminidase
FEBS Letters, 1993Co-Authors: Sangeeta Bagai, Debi P. SarkarAbstract:<B>HygromycinB> B was encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes). IncuBation of loaded F-virosomes with cultured HepG2 cells resulted in fusion mediated delivery of <B>HygromycinB> B to the cell cytoplasm, as was inferred from inhiBition of DNA synthesis. Binding of the F-virosomes to HepG2 cells was mediated By the interaction of terminal β-galactose residues of fusion protein with asialoglycoprotein receptor on HepG2 cells, suBsequently leading to fusion Between the two memBranes. The cytotoxic effect of <B>HygromycinB> B enclosed in F-virosomes was comparaBle with that of F,HN-virosomes containing Both hemagglutinin-neuraminidase (HN) and F protein and F,HNred-virosomes containing HN whose disulfide Bonds were irreversiBly reduced (HNred). <B>HygromycinB> B loaded fusogenic liposomes were prepared By coreconstituting the viral envelope containing only fusion protein with exogenous lipids. These fusogenic liposomes were found to Be more active than F-virosomes at the same fusion protein concentrations.
Sangeeta Bagai - One of the best experts on this subject based on the ideXlab platform.
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Targeted delivery of <B>HygromycinB> B using reconstituted Sendai viral envelopes lacking hemagglutinin‐neuraminidase
FEBS letters, 1993Co-Authors: Sangeeta Bagai, Debi P. SarkarAbstract:<B>HygromycinB> B was encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes). IncuBation of loaded F-virosomes with cultured HepG2 cells resulted in fusion mediated delivery of <B>HygromycinB> B to the cell cytoplasm, as was inferred from inhiBition of DNA synthesis. Binding of the F-virosomes to HepG2 cells was mediated By the interaction of terminal β-galactose residues of fusion protein with asialoglycoprotein receptor on HepG2 cells, suBsequently leading to fusion Between the two memBranes. The cytotoxic effect of <B>HygromycinB> B enclosed in F-virosomes was comparaBle with that of F,HN-virosomes containing Both hemagglutinin-neuraminidase (HN) and F protein and F,HNred-virosomes containing HN whose disulfide Bonds were irreversiBly reduced (HNred). <B>HygromycinB> B loaded fusogenic liposomes were prepared By coreconstituting the viral envelope containing only fusion protein with exogenous lipids. These fusogenic liposomes were found to Be more active than F-virosomes at the same fusion protein concentrations.
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targeted delivery of <B>HygromycinB> B using reconstituted sendai viral envelopes lacking hemagglutinin neuraminidase
FEBS Letters, 1993Co-Authors: Sangeeta Bagai, Debi P. SarkarAbstract:<B>HygromycinB> B was encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes). IncuBation of loaded F-virosomes with cultured HepG2 cells resulted in fusion mediated delivery of <B>HygromycinB> B to the cell cytoplasm, as was inferred from inhiBition of DNA synthesis. Binding of the F-virosomes to HepG2 cells was mediated By the interaction of terminal β-galactose residues of fusion protein with asialoglycoprotein receptor on HepG2 cells, suBsequently leading to fusion Between the two memBranes. The cytotoxic effect of <B>HygromycinB> B enclosed in F-virosomes was comparaBle with that of F,HN-virosomes containing Both hemagglutinin-neuraminidase (HN) and F protein and F,HNred-virosomes containing HN whose disulfide Bonds were irreversiBly reduced (HNred). <B>HygromycinB> B loaded fusogenic liposomes were prepared By coreconstituting the viral envelope containing only fusion protein with exogenous lipids. These fusogenic liposomes were found to Be more active than F-virosomes at the same fusion protein concentrations.
Editte Gharakhanian - One of the best experts on this subject based on the ideXlab platform.
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<B>HygromycinB> B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.
Current genetics, 2016Co-Authors: Daniele E. Ejzykowicz, Kristopher M. Locken, Fiona Ruiz, Surya P. Manandhar, Daniel K. Olson, Editte GharakhanianAbstract:Saccharomyces cerevisiae vacuoles are functionally analogous to mammalian lysosomes. Both also serve as physical platforms for Tor Complex 1 (TORC1) signal transduction, the master regulator of cellular growth and proliferation. <B>HygromycinB> B is a eukaryotic translation inhiBitor. We recently reported on <B>HygromycinB> B hypersensitive (hhy) mutants that fail to grow at suBtranslation inhiBitory concentrations of the drug and exhiBit vacuolar defects (Banuelos et al. in Curr Genet 56:121–137, 2010). Here, we show that hhy phenotype is not due to increased sensitivity to translation inhiBition and estaBlish a super HHY (s-HHY) suBgroup of genes comprised of ARF1, CHC1, DRS2, SAC1, VPS1, VPS34, VPS45, VPS52, and VPS54 that function exclusively or inclusively at trans-Golgi and late endosome interface. Live cell imaging of s-hhy mutants revealed that <B>HygromycinB> B treatment disrupts vacuolar morphology and the localization of late endosome marker Pep12, But not that of late endosome-independent vacuolar SNARE Vam3. This, along with normal post-late endosome trafficking of the vital dye FM4-64, estaBlishes that severe hypersensitivity to <B>HygromycinB> B correlates specifically with compromised trans-Golgi and late endosome interface. We also show that Tor1p vacuolar localization and TORC1 anaBolic functions, including growth promotion and phosphorylation of its direct suBstrate Sch9, are compromised in s-hhy mutants. Thus, an intact trans-Golgi and late endosome interface is a requisite for efficient Tor1 vacuolar localization and TORC1 function.
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Genomic analysis of severe hypersensitivity to <B>HygromycinB> B reveals linkage to vacuolar defects and new vacuolar gene functions in Saccharomyces cerevisiae
Current Genetics, 2010Co-Authors: M. G. Banuelos, D. E. Moreno, D. K. Olson, Q. Nguyen, F. Ricarte, C. R. Aguilera-sandoval, Editte GharakhanianAbstract:The vacuole of Saccharomyces cerevisiae has Been a seminal model for studies of lysosomal trafficking, Biogenesis, and function. Several yeast mutants defective in such vacuolar events have Been unaBle to grow at low levels of <B>HygromycinB> B, an aminoglycoside antiBiotic. We hypothesized that such severe hypersensitivity to <B>HygromycinB> B ( hhy ) is linked to vacuolar defects and performed a genomic screen for the phenotype using a haploid deletion strain liBrary of non-essential genes. Fourteen HHY genes were initially identified and were suBjected to Bioinformatics analyses. The uncovered hhy mutants were experimentally characterized with respect to vesicular trafficking, vacuole morphology, and growth under various stress and drug conditions. The comBination of Bioinformatics analyses and phenotypic characterizations implicate defects in vesicular trafficking, vacuole fusion/fission, or vacuole function in all hhy mutants. The collection was enriched for sensitivity to monensin, indicative of vacuolar trafficking defects. Additionally, all hhy mutants showed severe sensitivities to rapamycin and caffeine, suggestive of TOR kinase pathway defects. Our experimental results also estaBlish a new role in vacuolar and vesicular functions for two genes: PAF1 , encoding a RNAP II-associated protein required for expression of cell cycle-regulated genes, and TPD3 , encoding the regulatory suBunit of protein phosphatase 2A. Thus, our results support linkage Between severe hypersensitivity to <B>HygromycinB> B and vacuolar defects.
L Ventura - One of the best experts on this subject based on the ideXlab platform.
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Transformation of Aspergillus terreus with the <B>HygromycinB> B resistance marker from Escherichia coli.
FEMS microbiology letters, 1991Co-Authors: L Ventura, D RamónAbstract:Aspergillus terreus was transformed to <B>HygromycinB> B resistance using a Bacterial resistance gene under the control of Aspergillus nidulans regulatory sequences. Southern hyBridization of transformants indicated that in most of the cases the vector DNA was integrated into the recipient chromosome in the form of tandem arrays. Transformants were mitotically staBle in Both selective and non-selective medium and retained their capacity to produce xylanase or glucoamylase activities.
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Research letterTransformation of Aspergillus terreus with the <B>HygromycinB> B resistance marker from Escherichia coli
Fems Microbiology Letters, 1991Co-Authors: L Ventura, Daniel RamónAbstract:Aspergillus terruss was transformed to <B>HygromycinB> B resistance using a Bacterial resistance gene under the control of Aspergillus nidulans regulatory sequences. Southern hyBridization of transformants indicated that in most of the cases the vector DNA was integrated into the recipient chromosome in the form of tandem arrays. Transformants were mitotically staBle in Both selective and non-selective medium and retained their capacity to produce xylanase or glucoamylase activities.
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Transformation of Aspergillus terreus with the <B>HygromycinB> B resistance marker from Escherichia coli.
FEMS Microbiology Letters, 1991Co-Authors: L Ventura, Daniel RamónAbstract:Aspergillus terruss was transformed to <B>HygromycinB> B resistance using a Bacterial resistance gene under the control of Aspergillus nidulans regulatory sequences. Southern hyBridization of transformants indicated that in most of the cases the vector DNA was integrated into the recipient chromosome in the form of tandem arrays. Transformants were mitotically staBle in Both selective and non-selective medium and retained their capacity to produce xylanase or glucoamylase activities.
R Anderson - One of the best experts on this subject based on the ideXlab platform.
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<B>HygromycinB> B inhiBits synthesis of murine coronavirus RNA.
Antimicrobial agents and chemotherapy, 1991Co-Authors: G Macintyre, D E Woods, R AndersonAbstract:The aminoglycoside <B>HygromycinB> B inhiBits the infection of mouse hepatitis virus (MHV) A59 Both in vitro and in vivo. In proBing the mechanism By which <B>HygromycinB> B exerts its antiviral effect, we descriBe here studies which point to inhiBition of viral RNA synthesis as the key step in virus replication which is affected By the drug. Cells which are infected with MHV do not take up higher levels of <B>HygromycinB> B than do uninfected ones. Comparative assays of MHV replication and MHV protein synthesis in the presence of <B>HygromycinB> B and another aminoglycoside, neomycin, indicate that <B>HygromycinB> B is the more-effective antiviral agent and that its antiviral activity likely does not involve phosphoinositide-mediated processes such as those inhiBited By neomycin.
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<B>HygromycinB> B therapy of a murine coronaviral hepatitis.
Antimicrobial agents and chemotherapy, 1991Co-Authors: G Macintyre, B Curry, Fred Wong, R AndersonAbstract:Hepatitis caused By mouse hepatitis virus (MHV-A59), a murine coronavirus, is accompanied By direct infection and replication of virus within the liver. We demonstrate here that the aminoglycoside <B>HygromycinB> B is aBle to eliminate MHV-A59 infection from mouse peritoneal macrophages and cultured liver cells in vitro and is also aBle to reduce levels of virus replication and necrotic liver foci in vivo.
