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M.-r. Sierakowski - One of the best experts on this subject based on the ideXlab platform.

  • Xyloglucans from Hymenaea courbaril var. stilbocarpa seeds affect Arabidopsis thaliana seedling growth by enhancing lateral root development
    Revista Brasileira de Botânica, 2010
    Co-Authors: Adriana Tourinho Salamoni, M.-r. Sierakowski, Mondher El Jaziri, Marguerite M. Quoirin
    Abstract:

    The effect of crude xyloglucan (XG) preparations from jatoba (Hymenaea courbaril var. stilbocarpa (Hayne) Y. T. Lee & Langenh.) seeds on Arabidopsis thaliana (L.) Heynh. root system development was investigated. The XG extracts exerted a dual effect on root system development by slowing down root growth and improving lateral root formation. These observed morphological changes were not due to oligosaccharides that could be generated following hydrolysis of the XG polymers, since XG hydrolysate induced a drastic inhibition of the overall growth process of the Arabidopsis thaliana seedlings. Histochemical test of GUS gene expression assay performed on seven and 14-days-old transgenic Arabidopsis thaliana plants carrying the CycB1;1-GUS fusion indicated that the improvement of the lateral root development by jatoba XG extracts was not correlated with the expression of this cell cycle marker gene in the root system. A potential agricultural application of jatoba seeds XG extract is discussed.

  • Specific modification of xyloglucan from Hymenaea courbaril seeds
    Materials Science and Engineering: C, 2009
    Co-Authors: Neoli Lucyszyn, Adriana F. Lubambo, K.f. Matos, I. Marvilla, Clayton F. De Souza, M.-r. Sierakowski
    Abstract:

    Abstract In this work, xyloglucan extracted from the seeds of Hymenaea courbaril (jatoba) collected in Rancho Alegre, Parana, Brazil (JRAXG), was oxidized at the C-6 position of galactose or glucose units, using 2,3,6,6-tetramethylpiperidine-1-oxyl, to form uronic acid-containning polysaccharides (JRAOXGs), with different degrees of oxidation (10, 23 and 34%) and high yield (~ 95%). The properties of the news polyelectrolyte were studied by intrinsic viscosity, Gel Permeation Chromatography (GPC), Infrared spectroscopy (FTIR), 13C-NMR (13C-Nuclear Magnetic Resonance), and by Atomic Force Microscopy (AFM). The FTIR and 13C-NMR spectra confirmed the oxidation process. In comparison to JRA XG the JRAOXGs derivatives showed by GPC a homogeneous profile, but with hydrodynamic volume and molecular weight (Mw) reduction. Furthermore, AFM images on mica, after dewetting hydrodynamic volume, show different states of aggregation accordingly to the degree of oxidation when compared to the native state. The native XG presents a rather compact and quite homogeneous topography with a few holes and absence of aggregates, while the oxidation products present spherical-like conformation whose diameters is in the range of 8–30 nm. The morphological changes observed by AFM to JRAOXG34 derivative, can be very important to guide to drug encapsulation, for example.

  • physico chemical aspects of galactoxyloglucan from the seeds of Hymenaea courbaril and its tetraborate complex
    Carbohydrate Polymers, 2003
    Co-Authors: S Martin, Rilton Alves De Freitas, E Obayashi, M.-r. Sierakowski
    Abstract:

    A galactoxyloglucan hydrocolloid (HXGRP) (Glu:Xyl:Gal molar ratio, ∼4:3:1) was obtained from cotyledons of seeds of Hymenaea courbaril by aqueous extraction and then purified (33% yield). Rheological measurements showed that HXGRP, in aqueous systems, interacts with tetraborate ions increasing both, viscosity and G′ values, also increasing the viscoelasticity of the system, but not forming a gel, as shown by Cox-Merz experiments. This interaction dependent of the amount of sodium tetraborate and the pH, and was analyzed in terms of hindered reptation. A temperature sweep of the HXGRP-tetraborate complex showed a characteristic cleavage of cross-links between polysaccharide and borate ions at high temperatures. An aqueous solution of HXGRP after heating at 85 °C for 2 h showed a decrease in its absolute viscosity.

  • Physico–chemical aspects of galactoxyloglucan from the seeds of Hymenaea courbaril and its tetraborate complex
    Carbohydrate Polymers, 2003
    Co-Authors: S Martin, Rilton Alves De Freitas, E Obayashi, M.-r. Sierakowski
    Abstract:

    A galactoxyloglucan hydrocolloid (HXGRP) (Glu:Xyl:Gal molar ratio, ∼4:3:1) was obtained from cotyledons of seeds of Hymenaea courbaril by aqueous extraction and then purified (33% yield). Rheological measurements showed that HXGRP, in aqueous systems, interacts with tetraborate ions increasing both, viscosity and G′ values, also increasing the viscoelasticity of the system, but not forming a gel, as shown by Cox-Merz experiments. This interaction dependent of the amount of sodium tetraborate and the pH, and was analyzed in terms of hindered reptation. A temperature sweep of the HXGRP-tetraborate complex showed a characteristic cleavage of cross-links between polysaccharide and borate ions at high temperatures. An aqueous solution of HXGRP after heating at 85 °C for 2 h showed a decrease in its absolute viscosity.

  • Xyloglucan octasaccharide XXLGol derived from the seeds of Hymenaea courbaril acts as a signaling molecule
    Plant physiology, 1998
    Co-Authors: Carem Gledes Vargas-rechia, Fany Reicher, M.-r. Sierakowski, Alain Heyraud, Hugues Driguez, Yvette Lienart
    Abstract:

    Treatment of the xyloglucan isolated from the seeds of Hymenaea courbaril with Humicola insolens endo-1,4-β-d-glucanase I produced xyloglucan oligosaccharides, which were then isolated and characterized. The two most abundant compounds were the heptasaccharide (XXXG) and the octasaccharide (XXLG), which were examined by reference to the biological activity of other structurally related xyloglucan compounds. The reduced oligomer (XXLGol) was shown to promote growth of wheat ( Triticum aestivum ) coleoptiles independently of the presence of 2,4-dichlorophenoxyacetic acid (2,4-D). In the presence of 2,4-D, XXLGol at nanomolar concentrations increased the auxin-induced response. It was found that XXLGol is a signaling molecule, since it has the ability to induce, at nanomolar concentrations, a rapid increase in an α-l-fucosidase response in suspended cells or protoplasts of Rubus fruticosus L. and to modulate 2,4-D or gibberellic acid-induced α-l-fucosidase.

Marcos Silveira Buckeridge - One of the best experts on this subject based on the ideXlab platform.

  • Diurnal changes in storage carbohydrate metabolism in cotyledons of the tropical tree Hymenaea courbaril L. (Leguminosae)
    Brazilian Journal of Botany, 2012
    Co-Authors: Lourdes Isabel Velho Do Amaral, Henrique Pereira Dos Santos, Davi Rodrigo Rossatto, Marcos Silveira Buckeridge
    Abstract:

    The cotyledons of Hymenaea courbaril store large amounts of xyloglucan, a cell wall polysaccharide that is believed to serve as storage for the period of seedling establishment. During storage mobilisation, xyloglucan seems to be degraded by a continuous process that starts right after radicle protrusion and follows up to the establishment of photosynthesis. Here we show evidence that events related to the hydrolases activities and production (α-xylosidase, β-galactosidase, β-glucosidase and xyloglucan endo-β-transglucosilase) as well as auxin, showed changes that follow the diurnal cycle. The period of higher hydrolases activities was between 6pm and 6am, which is out of phase with photosynthesis. Among the enzymes, α-xilosidase seems to be more important than β-glucosidase and β-galactosidase in the xyloglucan disassembling mechanism. Likewise, the sugars related with sucrose metabolism followed the rhythm of the hydrolases, but starch levels were shown to be practically constant. A high level of auxin was observed during the night, what is compatible with the hypothesis that this hormone would be one of the regulators of the whole process. The probable biological meaning of the existence of such a complex control mechanism during storage mobilisation is likely to be related to a remarkably high level of efficiency of carbon usage by the growing seedling of Hymenaea courbaril, allowing the establishment of very vigorous seedlings in the tropical forest.

  • Fine structure of a mixed-oligomer storage xyloglucan from seeds of Hymenaea courbaril
    Carbohydrate Polymers, 2006
    Co-Authors: Marco Aurélio Silva Tiné, Clovis Oliveira Silva, Denis Ubeda De Lima, Nicholas C. Carpita, Marcos Silveira Buckeridge
    Abstract:

    Seed storage xyloglucans of Hymenaea courbaril possess a structure composed of xylocellopentaosyl (XXXXG) and xylocellohexaosyl (XXXXXG) backbone units in addition to the more common xylocellotetraosyl (XXXG) units. Electrospray mass spectrometry confirmed that both types of units exist in the same polymer. The storage xyloglucan gives a xylocellotetraose:xylocellopentaose:xylocellohexaose ratio of about 2:1:0.2. Incomplete digestion of xyloglucan gave dimers of these units in ratios of tetramer–tetramer (T-T), tetramer–pentamer (T-P), and pentamer–pentamer (P-P) backunits of 3.1:2.1:1.0, far from the 4:4:1 ratio expected if the assembly were random. Although discovery of the xylocellopentaosyl units requires a re-evaluation of the cellobiosyl/cellotetraosyl unit mechanism of backbone synthesis, a 4–5–5–4 unit ordered assembly that accounts for the dimer ratios observed preserves a mechanism by which the even-number cellodextrin units are added. Three distinct, singly galactosylated oligomers from the xylocellopentaosyl units were isolated and characterized, with XXXLG being the predominant oligomer of this type in the polymer. Examination of galactosylation profiles of the dimers indicates that the position of these subtending residues is also not random.

  • The control of storage xyloglucan mobilization in cotyledons of Hymenaea courbaril.
    Plant physiology, 2004
    Co-Authors: Henrique Pessoa Dos Santos, Eduardo Purgatto, Helenice Mercier, Marcos Silveira Buckeridge
    Abstract:

    Hymenaea courbaril is a leguminous tree species from the neotropical rain forests. Its cotyledons are largely enriched with a storage cell wall polysaccharide (xyloglucan). Studies of cell wall storage polymers have been focused mostly on the mechanisms of their disassembly, whereas the control of their mobilization and the relationship between their metabolism and seedling development is not well understood. Here, we show that xyloglucan mobilization is strictly controlled by the development of first leaves of the seedling, with the start of its degradation occurring after the beginning of eophyll (first leaves) expansion. During the period of storage mobilization, an increase in the levels of xyloglucan hydrolases, starch, and free sugars were observed in the cotyledons. Xyloglucan mobilization was inhibited by shoot excision, darkness, and by treatment with the auxin-transport inhibitor N-1-naphthylphthalamic acid. Analyses of endogenous indole-3-acetic acid in the cotyledons revealed that its increase in concentration is followed by the rise in xyloglucan hydrolase activities, indicating that auxin is directly related to xyloglucan mobilization. Cotyledons detached during xyloglucan mobilization and treated with 2,4-dichlorophenoxyacetic acid showed a similar mobilization rate as in attached cotyledons. This hormonal control is probably essential for the ecophysiological performance of this species in their natural environment since it is the main factor responsible for promoting synchronism between shoot growth and reserve degradation. This is likely to increase the efficiency of carbon reserves utilization by the growing seedling in the understorey light conditions of the rain forest.

  • Effect of atmospheric CO2 enrichment on the establishment of seedlings of Jatobá, Hymenaea courbaril L. (Leguminosae, Caesalpinioideae)
    Biota Neotropica, 2002
    Co-Authors: Marcos Pereira Marinho Aidar, Carlos Alberto Martinez, A.c. Costa, P.m.f. Costa, S.m.c. Dietrich, Marcos Silveira Buckeridge
    Abstract:

    Plântulas de jatoba crescidas em ambiente com concentracoes elevadas de CO2 podem exibir aclimatacao fotossintetica ou retro-inibicao, a qual e caracterizada pela reducao das taxas fotossinteticas. Em muitos casos de aclimatacao induzida por alto CO2, taxas reduzidas de fotossintese sao observadas, mas ainda sao mais altas que aquelas detectadas em plantas crescendo em concentracoes atuais de CO2 atmosferico (360ppm). No presente trabalho, realizamos um estudo do comportamento de plântulas de Hymenaea courbaril, uma especie secundaria tardia/climax de grande importância em florestas tropicais maduras nas Americas. Em nossos experimentos, sementes recem germinadas foram cultivadas em câmaras de topo aberto com concentracoes alteradas de CO2 (720 ppm e controle a 340 ppm). Para testar os efeitos da presenca de compostos de reserva sobre as respostas de plântulas em crescimento, cotiledones foram destacados antes do inicio da mobilizacao do polissacarideo de reserva (xiloglucano) e parâmetros tais como massa seca, area foliar, assimilacao de CO2 e fluorescencia da clorofila a, foram medidos durante 98 dias. A comparacao entre plântulas crescendo em atmosfera de CO2 a 360 e 720 ppm, mostraram aumento siginificativo em area foliar apenas nos metafilos de plântulas crescendo nas concentracoes mais altas. No entanto, um efeito marcado e consistente de aumento na fotossintese (assimilacao de CO2) foi observado em todos os casos (com e sem cotiledones). Acredita-se que as variacoes nos niveis de sacarose podem funcionar como um mecanismo sinalizador de alteracoes nos estados de armazenamento ou desenvolvimento de tecidos vegetais. Portanto, uma possivel explicacao para nossas observacoes gerais de que uma resposta diferencial em termos de crescimento de plântulas deixa de existir quando a mobilizacao de reservas esta em curso, pode estar relacionada com o fato de que altos niveis de sacarose sao produzidos quando ha degradacao de compostos de reservas. De acordo com nossos resultados, parece que as plantas crescidas sob concentracoes enriquecidas de CO2 nao aclimataram e portanto, sob as condicoes climaticas previstas com base nos niveis atmosfericos atuais, plântulas de Hymenaea courbaril deverao estabelecer mais rapidamente em seu ambiente natural e podem tambem servir como um mecanismo eficiente de sequestro de carbono pela floresta.schottiana Mart.. Phytoseiidae foi a segunda familia mais numerosa (11,2% dos acaros coletados), sendo a mais abundante em foliolos.

  • Xyloglucan mobilisation in cotyledons of developing plantlets of Hymenaea courbaril L. (Leguminosae-Caesalpinoideae)
    Plant science : an international journal of experimental plant biology, 2000
    Co-Authors: Marco Aurélio Silva Tiné, Angelo Luiz Cortelazzo, Marcos Silveira Buckeridge
    Abstract:

    Many seeds contain storage compounds that are used by the embryo/plantlet as a source of nutrients after germination. In seeds of Hymenaea courbaril, a leguminous tree, the main reserve consists of a structurally unusual xyloglucan stored in thickened walls of the cotyledon cells. The present work aimed to study H. courbaril xyloglucan metabolism during and after germination in order to compare its degrading system with the other known xyloglucan containing seeds. Polysaccharide degradation occurred after germination between 35 and 55 days after planting. The activities of α-xylosidase, β-glucosidase, β-galactosidase and XET rose during the period of xyloglucan disassembling but a low level of endo-β-glucanase activity was detected, suggesting that this XET has high affinity for the oligosaccharides. The pH optimum of β-galactosidase was different from the α-xylosidase, β-glucosidase and XET optima suggesting that the former may be important in the control of the mobilisation process. A tentative model for xyloglucan disassembling in vivo is proposed, where β-galactosidase allows the free oligosaccharides to bypass a transglycosylation cycle and be disassembled by the other exo-enzymes. Some ecophysiological comparisons among H. courbaril and other xyloglucan storing seeds are discussed.

Maria Do Rosário Rodrigues Silva - One of the best experts on this subject based on the ideXlab platform.

  • Antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril L. and its major constituent fisetin.
    BMC complementary and alternative medicine, 2014
    Co-Authors: Maysa Paula Da Costa, Marize Campos Valadares Bozinis, Wanessa Machado Andrade, Carolina Rodrigues Costa, Alessandro Lopes Da Silva, Cecília M.a. De Oliveira, Lucilia Kato, Orionalda De Fátima Lisboa Fernandes, Lúcia Kioko Hasimoto E Souza, Maria Do Rosário Rodrigues Silva
    Abstract:

    The great potential of plants as Hymenaea courbaril L (jatoba) has not yet been throughly explored scientifically and therefore it is very important to investigate their pharmacological and toxicological activities to establish their real efficacy and safety. This study investigated the cytotoxicity of xylem sap of Hymenaea courbaril L and its bioactivity against the fungi Cryptococcus neoformans species complex and dermatophytes. The fresh xylem sap of H. courbaril was filtered resulting in an insoluble brown color precipitate and was identified as fisetin. In the filtrate was identified the mixture of fisetinediol, fustin, 3-O-methyl-2,3-trans-fustin and taxifolin, which were evaluated by broth microdilution antifungal susceptibility testing against C. neoformans species complex and dermatophytes. The fresh xylem sap and fisetin were screened for cytotoxicity against the 3T3-A31 cells of Balb/c using neutral red uptake (NRU) assay. The fresh xylem sap and the fisetin showed higher in vitro activity than the filtrate. The xylem sap of H. courbaril inhibited the growth of dermatophytes and of C. neoformans with minimal inhibition concentration (MIC) 

  • antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril l and its major constituent fisetin
    BMC Complementary and Alternative Medicine, 2014
    Co-Authors: Maysa Paula Da Costa, Marize Campos Valadares Bozinis, Wanessa Machado Andrade, Carolina Rodrigues Costa, Alessandro Lopes Da Silva, Cecília M.a. De Oliveira, Lucilia Kato, Orionalda De Fátima Lisboa Fernandes, Lucia Kioko Hasimoto E Souza, Maria Do Rosário Rodrigues Silva
    Abstract:

    The great potential of plants as Hymenaea courbaril L (jatoba) has not yet been throughly explored scientifically and therefore it is very important to investigate their pharmacological and toxicological activities to establish their real efficacy and safety. This study investigated the cytotoxicity of xylem sap of Hymenaea courbaril L and its bioactivity against the fungi Cryptococcus neoformans species complex and dermatophytes. The fresh xylem sap of H. courbaril was filtered resulting in an insoluble brown color precipitate and was identified as fisetin. In the filtrate was identified the mixture of fisetinediol, fustin, 3-O-methyl-2,3-trans-fustin and taxifolin, which were evaluated by broth microdilution antifungal susceptibility testing against C. neoformans species complex and dermatophytes. The fresh xylem sap and fisetin were screened for cytotoxicity against the 3T3-A31 cells of Balb/c using neutral red uptake (NRU) assay. The fresh xylem sap and the fisetin showed higher in vitro activity than the filtrate. The xylem sap of H. courbaril inhibited the growth of dermatophytes and of C. neoformans with minimal inhibition concentration (MIC) < 256 μg/mL, while the fisetin showed MIC < 128 μg/mL for these fungi. Fisetin showed lower toxicity (IC50 = 158 μg/mL) than the fresh xylem sap (IC50 = 109 μg/mL). Naturally occurring fisetin can provide excellent starting points for clinical application and can certainly represent a therapeutic potential against fungal infections, because it showed in vitro antifungal activity and low toxicity on animal cells.

Fany Reicher - One of the best experts on this subject based on the ideXlab platform.

  • Rheological properties of thermally xyloglucan gel from the seeds of Hymenaea courbaril
    Materials Science and Engineering: C, 2009
    Co-Authors: Ana P. Busato, Fany Reicher, Rafael Campos Serra Domingues, Joana Léa Meira Silveira
    Abstract:

    Abstract A Brazilian source of galactoxyloglucan was obtained from seeds of Hymenaea courbaril and investigated before (JN) and after the hydrolytic removal of galactose (JH). The native polysaccharide contained glucose, xylose and galactose in a ~ 4.0:2.7:1 molar ratio. Gelation occurred when ~ 51% of the galactose residues was removed from the xyloglucan by fungal β-galactosidase. The rheological properties of JN and JH were analyzed. Dynamic oscillatory measurements characterized JN as a viscoelastic solution and JH as a true gel. JH achieved the maximum storage modulus G′ at temperatures above 40 °C and was found to increase with the increasing concentration. The gel point temperatures of JH fraction were determined at the cross-over point of G′ and G″ and by calculating the inflexion point of the G′ plot.

  • New 4-O-substituted xylosyl units in the xyloglucan from leaves of Hymenaea courbaril.
    International journal of biological macromolecules, 2005
    Co-Authors: Ana P. Busato, Carem Gledes Vargas-rechia, Philip A.j. Gorin, Carmen Lúcia De Oliveira Petkowicz, Cesar A. Tischer, Renato Bochicchio, Fany Reicher
    Abstract:

    Abstract A homogeneous fucogalactoxyloglucan, isolated from the leaves of Hymenaea courbaril , was analysed by methylation-GC–MS. These procedures involved derived partially O -methylated alditol acetates and acetylated aldononitriles, which demonstrated the presence of both 2- O - and 4- O -substituted Xyl p units in the side-chains. The presence of the unusual, latter structure was confirmed by 2D NMR spectroscopy with a correlated HMQC C-4/H-4 signal at δ 77.8/3.73. A similar 4- O -substituted xylosyl structure was present in a decasaccharide Glc 4 Xyl 3 Gal 2 Fuc obtained via endo -glucanase treatment of the polysaccharide, which gave rise to a molecular ion with m / z 1555 (ESI-MS, Na + form).

  • Xyloglucan from the leaves of Hymenaea courbaril.
    Phytochemistry, 2001
    Co-Authors: Ana P. Busato, Carem Gledes Vargas-rechia, Fany Reicher
    Abstract:

    Abstract A fucosylated xyloglucan was isolated from the leaves of Hymenaea courbaril by alkaline extraction, followed by ethanol precipitation and ion-exchange chromatography. The isolated polysaccharide showed Glc:Xyl:Gal:Fuc in molar ratio of 8:5:2.5:1 and α D 25 +40.5°. Composition and linkage analyses, supported by NMR spectroscopic measurements, showed that the polysaccharide has a glucan backbone which is highly substituted at O -6 with d -xylopyranose residues, about a half of which are substituted at O -2 by d -galactopyranosyl units. Some of the galactose residues are further substituted by l -fucopyranose at O -2. The M r , as determined by HPSEC, was 49,500.

  • Xyloglucan octasaccharide XXLGol derived from the seeds of Hymenaea courbaril acts as a signaling molecule
    Plant physiology, 1998
    Co-Authors: Carem Gledes Vargas-rechia, Fany Reicher, M.-r. Sierakowski, Alain Heyraud, Hugues Driguez, Yvette Lienart
    Abstract:

    Treatment of the xyloglucan isolated from the seeds of Hymenaea courbaril with Humicola insolens endo-1,4-β-d-glucanase I produced xyloglucan oligosaccharides, which were then isolated and characterized. The two most abundant compounds were the heptasaccharide (XXXG) and the octasaccharide (XXLG), which were examined by reference to the biological activity of other structurally related xyloglucan compounds. The reduced oligomer (XXLGol) was shown to promote growth of wheat ( Triticum aestivum ) coleoptiles independently of the presence of 2,4-dichlorophenoxyacetic acid (2,4-D). In the presence of 2,4-D, XXLGol at nanomolar concentrations increased the auxin-induced response. It was found that XXLGol is a signaling molecule, since it has the ability to induce, at nanomolar concentrations, a rapid increase in an α-l-fucosidase response in suspended cells or protoplasts of Rubus fruticosus L. and to modulate 2,4-D or gibberellic acid-induced α-l-fucosidase.

  • OLIGOSACCHARIDES DERIVED FROM THE XYLOGLUCAN ISOLATED FROM THE SEEDS OF Hymenaea courbaril VAR. STILBOCARPA
    International journal of biological macromolecules, 1995
    Co-Authors: Nery N. Lima, Fany Reicher, Carem Gledes Vargas Rechia, J.l.m.s Ganter, M.-r. Sierakowski
    Abstract:

    On aqueous extraction, Hymenaea courbaril var. stilbocarpa, known in Brazil as jatoba, furnishes a high yield of viscous xyloglucan (45%) from its seeds. The crude polysaccharide (B1) was hydrolysed and the products, analysed as alditol acetates, were glucose, xylose, galactose and arabinose in the ratio 50:35:13:2. After further fractionation on DEAE-cellulose column (chloride form), the main fraction (70% yield, B2) was obtained. The basic structure of the xyloglucan was determined as a cellulose-type (1-->4)-linked beta-D-glucan backbone partially substituted with side chains at O6 of alpha-D-xylopyranose, some of which were themselves substituted at O2 by the units of beta-D-galactopyranose. Treatment of the xyloglucan (B2) with commercial cellulase from Trichoderma sp. yielded six oligosaccharides. These oligosaccharides were isolated by preparative paper chromatography, and their structures were determined by gas-liquid chromatography-mass spectroscopy of the derived partially O-methylated alditol acetates. These results confirm the structure proposed for jatoba seed xyloglucan.

Maysa Paula Da Costa - One of the best experts on this subject based on the ideXlab platform.

  • Antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril L. and its major constituent fisetin.
    BMC complementary and alternative medicine, 2014
    Co-Authors: Maysa Paula Da Costa, Marize Campos Valadares Bozinis, Wanessa Machado Andrade, Carolina Rodrigues Costa, Alessandro Lopes Da Silva, Cecília M.a. De Oliveira, Lucilia Kato, Orionalda De Fátima Lisboa Fernandes, Lúcia Kioko Hasimoto E Souza, Maria Do Rosário Rodrigues Silva
    Abstract:

    The great potential of plants as Hymenaea courbaril L (jatoba) has not yet been throughly explored scientifically and therefore it is very important to investigate their pharmacological and toxicological activities to establish their real efficacy and safety. This study investigated the cytotoxicity of xylem sap of Hymenaea courbaril L and its bioactivity against the fungi Cryptococcus neoformans species complex and dermatophytes. The fresh xylem sap of H. courbaril was filtered resulting in an insoluble brown color precipitate and was identified as fisetin. In the filtrate was identified the mixture of fisetinediol, fustin, 3-O-methyl-2,3-trans-fustin and taxifolin, which were evaluated by broth microdilution antifungal susceptibility testing against C. neoformans species complex and dermatophytes. The fresh xylem sap and fisetin were screened for cytotoxicity against the 3T3-A31 cells of Balb/c using neutral red uptake (NRU) assay. The fresh xylem sap and the fisetin showed higher in vitro activity than the filtrate. The xylem sap of H. courbaril inhibited the growth of dermatophytes and of C. neoformans with minimal inhibition concentration (MIC) 

  • antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril l and its major constituent fisetin
    BMC Complementary and Alternative Medicine, 2014
    Co-Authors: Maysa Paula Da Costa, Marize Campos Valadares Bozinis, Wanessa Machado Andrade, Carolina Rodrigues Costa, Alessandro Lopes Da Silva, Cecília M.a. De Oliveira, Lucilia Kato, Orionalda De Fátima Lisboa Fernandes, Lucia Kioko Hasimoto E Souza, Maria Do Rosário Rodrigues Silva
    Abstract:

    The great potential of plants as Hymenaea courbaril L (jatoba) has not yet been throughly explored scientifically and therefore it is very important to investigate their pharmacological and toxicological activities to establish their real efficacy and safety. This study investigated the cytotoxicity of xylem sap of Hymenaea courbaril L and its bioactivity against the fungi Cryptococcus neoformans species complex and dermatophytes. The fresh xylem sap of H. courbaril was filtered resulting in an insoluble brown color precipitate and was identified as fisetin. In the filtrate was identified the mixture of fisetinediol, fustin, 3-O-methyl-2,3-trans-fustin and taxifolin, which were evaluated by broth microdilution antifungal susceptibility testing against C. neoformans species complex and dermatophytes. The fresh xylem sap and fisetin were screened for cytotoxicity against the 3T3-A31 cells of Balb/c using neutral red uptake (NRU) assay. The fresh xylem sap and the fisetin showed higher in vitro activity than the filtrate. The xylem sap of H. courbaril inhibited the growth of dermatophytes and of C. neoformans with minimal inhibition concentration (MIC) < 256 μg/mL, while the fisetin showed MIC < 128 μg/mL for these fungi. Fisetin showed lower toxicity (IC50 = 158 μg/mL) than the fresh xylem sap (IC50 = 109 μg/mL). Naturally occurring fisetin can provide excellent starting points for clinical application and can certainly represent a therapeutic potential against fungal infections, because it showed in vitro antifungal activity and low toxicity on animal cells.