The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform

Michael D. Southall - One of the best experts on this subject based on the ideXlab platform.

  • low level red led light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne
    Lasers in Surgery and Medicine, 2018
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P < 0.05) reduced following red LED light at 0.2, 0.5, and 1.2 J/cm2 , from 266 ± 11 pg/ml of no-light-plus-OA-treated (OA treatment without light) controls to 216 ± 9, 231 ± 8, and 212 ± 7 pg/ml, respectively. Histological examination showed that SC thickening following OA treatment was reduced from 43% of total epidermis for no-light-plus-OA treatment to 37% and 38% of total epidermis following 0.5 and 1.1 J/cm2 red light plus OA treatment, respectively (P < 0.05). Moreover, 1.1 J/cm2 red-light-plus-OA treatment improved OA-induced TEER changes from 29% of baseline for no-light-plus-OA treatment, to 36% of baseline. CONCLUSION Low level red LED light therapy could provide beneficial effects of anti-inflammation, normalizing pilosebaceous Hyperkeratinization, and improving barrier impairment in Acne vulgaris. Lasers Surg. Med. 50:158-165, 2018. © 2017 Wiley Periodicals, Inc.

  • Low-level red LED light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne.
    Lasers in surgery and medicine, 2017
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P 

  • In vitro modeling of unsaturated free fatty acid-mediated tissue impairments seen in acne lesions
    Archives of Dermatological Research, 2017
    Co-Authors: Wen-hwa Li, Qihong Zhang, Carol R. Flach, Richard Mendelsohn, Michael D. Southall, Ramine Parsa
    Abstract:

    Acne vulgaris is a disease of pilosebaceous units with multifactorial pathogenesis, including Hyperkeratinization, increased sebum secretion, and inflammation. Recently, it was suggested that acne subjects may have also impaired skin barrier. We hypothesized that excess unsaturated free fatty acids (UFFA) present in the sebum may cause barrier impairment associated with increased follicular stratum corneum (SC) thickening and inflammation seen in acne. Therefore, epidermal and sebaceous lipid profiles from acne and healthy subjects were analyzed and an in vitro epidermal tissue model was developed to validate this hypothesis. Significantly increased levels of free fatty acids ( p  

Ramine Parsa - One of the best experts on this subject based on the ideXlab platform.

  • low level red led light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne
    Lasers in Surgery and Medicine, 2018
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P < 0.05) reduced following red LED light at 0.2, 0.5, and 1.2 J/cm2 , from 266 ± 11 pg/ml of no-light-plus-OA-treated (OA treatment without light) controls to 216 ± 9, 231 ± 8, and 212 ± 7 pg/ml, respectively. Histological examination showed that SC thickening following OA treatment was reduced from 43% of total epidermis for no-light-plus-OA treatment to 37% and 38% of total epidermis following 0.5 and 1.1 J/cm2 red light plus OA treatment, respectively (P < 0.05). Moreover, 1.1 J/cm2 red-light-plus-OA treatment improved OA-induced TEER changes from 29% of baseline for no-light-plus-OA treatment, to 36% of baseline. CONCLUSION Low level red LED light therapy could provide beneficial effects of anti-inflammation, normalizing pilosebaceous Hyperkeratinization, and improving barrier impairment in Acne vulgaris. Lasers Surg. Med. 50:158-165, 2018. © 2017 Wiley Periodicals, Inc.

  • Low-level red LED light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne.
    Lasers in surgery and medicine, 2017
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P 

  • In vitro modeling of unsaturated free fatty acid-mediated tissue impairments seen in acne lesions
    Archives of Dermatological Research, 2017
    Co-Authors: Wen-hwa Li, Qihong Zhang, Carol R. Flach, Richard Mendelsohn, Michael D. Southall, Ramine Parsa
    Abstract:

    Acne vulgaris is a disease of pilosebaceous units with multifactorial pathogenesis, including Hyperkeratinization, increased sebum secretion, and inflammation. Recently, it was suggested that acne subjects may have also impaired skin barrier. We hypothesized that excess unsaturated free fatty acids (UFFA) present in the sebum may cause barrier impairment associated with increased follicular stratum corneum (SC) thickening and inflammation seen in acne. Therefore, epidermal and sebaceous lipid profiles from acne and healthy subjects were analyzed and an in vitro epidermal tissue model was developed to validate this hypothesis. Significantly increased levels of free fatty acids ( p  

Ali Fassih - One of the best experts on this subject based on the ideXlab platform.

  • low level red led light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne
    Lasers in Surgery and Medicine, 2018
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P < 0.05) reduced following red LED light at 0.2, 0.5, and 1.2 J/cm2 , from 266 ± 11 pg/ml of no-light-plus-OA-treated (OA treatment without light) controls to 216 ± 9, 231 ± 8, and 212 ± 7 pg/ml, respectively. Histological examination showed that SC thickening following OA treatment was reduced from 43% of total epidermis for no-light-plus-OA treatment to 37% and 38% of total epidermis following 0.5 and 1.1 J/cm2 red light plus OA treatment, respectively (P < 0.05). Moreover, 1.1 J/cm2 red-light-plus-OA treatment improved OA-induced TEER changes from 29% of baseline for no-light-plus-OA treatment, to 36% of baseline. CONCLUSION Low level red LED light therapy could provide beneficial effects of anti-inflammation, normalizing pilosebaceous Hyperkeratinization, and improving barrier impairment in Acne vulgaris. Lasers Surg. Med. 50:158-165, 2018. © 2017 Wiley Periodicals, Inc.

  • Low-level red LED light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne.
    Lasers in surgery and medicine, 2017
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P 

Curt Binner - One of the best experts on this subject based on the ideXlab platform.

  • low level red led light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne
    Lasers in Surgery and Medicine, 2018
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P < 0.05) reduced following red LED light at 0.2, 0.5, and 1.2 J/cm2 , from 266 ± 11 pg/ml of no-light-plus-OA-treated (OA treatment without light) controls to 216 ± 9, 231 ± 8, and 212 ± 7 pg/ml, respectively. Histological examination showed that SC thickening following OA treatment was reduced from 43% of total epidermis for no-light-plus-OA treatment to 37% and 38% of total epidermis following 0.5 and 1.1 J/cm2 red light plus OA treatment, respectively (P < 0.05). Moreover, 1.1 J/cm2 red-light-plus-OA treatment improved OA-induced TEER changes from 29% of baseline for no-light-plus-OA treatment, to 36% of baseline. CONCLUSION Low level red LED light therapy could provide beneficial effects of anti-inflammation, normalizing pilosebaceous Hyperkeratinization, and improving barrier impairment in Acne vulgaris. Lasers Surg. Med. 50:158-165, 2018. © 2017 Wiley Periodicals, Inc.

  • Low-level red LED light inhibits Hyperkeratinization and inflammation induced by unsaturated fatty acid in an in vitro model mimicking acne.
    Lasers in surgery and medicine, 2017
    Co-Authors: Ali Fassih, Ramine Parsa, Curt Binner, Michael D. Southall
    Abstract:

    BACKGROUND AND OBJECTIVE Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (Hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm2 have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm2 for acne using an in vitro model previously developed to mimic the inflammation and Hyperkeratinization observed clinically in acne. MATERIALS AND METHODS Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor Hyperkeratinization. RESULTS OA-induced IL-1α release was significantly (P 

Diane Thiboutot - One of the best experts on this subject based on the ideXlab platform.

  • Activity of 5-Alpha-Reductase and 17-Beta-Hydroxysteroid Dehydrogenase in the Infrainfundibulum of Subjects with and without Acne vulgaris
    Dermatology (Basel Switzerland), 1998
    Co-Authors: Diane Thiboutot, H. Knaggs, Kathyrn Gilliland, G. Lin
    Abstract:

    Linoleic acid deficiency, interleukin 1, retinoids and androgens have been implicated as causative factors in the follicular Hyperkeratinization seen in acne. The goal of this study was to test the hy

  • Activity of type 1 5α–reductase is greater in the follicular infrainfundibulum compared with the epidermis
    British Journal of Dermatology, 1997
    Co-Authors: Diane Thiboutot, H. Knaggs, Kathyrn Gilliland, S. Hagari
    Abstract:

    The enzyme 5 alpha-reductase converts testosterone (T) to dihydrotestosterone (DHT). Although this enzyme has been localized to various regions of the pilosebaceous unit, its activity has not been studied in the follicular portion of either vellus or sebaceous follicles. The goal of our study was to determine the relative activities of 5 alpha-reductase within various regions of these follicles with particular emphasis on the infrainfundibulum. A finding of increased 5 alpha-reductase activity in upper follicles compared to epidermis might support the hypothesis that increased follicular production of DHT is involved in the Hyperkeratinization observed in this region of the follicle in acne vulgaris. 5 alpha-reductase activity was determined at pH 5 (optimal for the type 2 isozyme) and pH 7 (optimal for the type 1 isozyme) in isolated infrainfundibular segments from sebaceous and vellus follicles, homogenized epidermis from various anatomical areas and in microdissected segments of the pilosebaceous unit from breast skin of normal subjects. Enzyme activity was also determined at pH 7 in cultured infrainfundibular keratinocytes and in interfollicular epidermal keratinocytes. Homogenates of infrainfundibular segments demonstrated significantly greater activity at pH 7 compared to pH 5 (P < 0.001), confirming activity of the type 1 5 alpha-reductase in this region. Activity of 5 alpha-reductase was much lower in homogenized epidermis and did not demonstrate a clear pH preference. Keratinocytes cultured from the infrainfundibulum demonstrated significantly greater 5 alpha-reductase activity compared to keratinocytes from interfollicular epidermis (P = 0.04). In the dissected segments of pilosebaceous units from breast skin, 5 alpha-reductase activity was greatest in the sebaceous gland followed by the sebaceous duct, infrainfundibulum, whole skin and epidermis. These data indicate that 5 alpha-reductase activity varies within regions of the pilosebaceous unit and compared with interfollicular epidermal cells, infrainfundibular keratinocytes have an increased capacity for producing androgens which may play a role in the follicular Hyperkeratinization seen in acne.

  • ACNE: An Overview of Clinical Research Findings
    Dermatologic clinics, 1997
    Co-Authors: Diane Thiboutot
    Abstract:

    Although acne represents the most common chronic skin condition seen by dermatologists, there are still many unanswered questions regarding its pathophysiology, and patients are still in need of more effective therapies, particularly those aimed at the hormonal aspects of acne. Recent clinical research has led to advances in our understanding of factors such as cytokines in follicular Hyperkeratinization and the role of androgens in acne, the emergence and significance of antibiotic resistance of P. acnes, the long-term safety and efficacy of isotretinoin, and the safety and efficacy of new topical retinoids, such as tazarotene and adapalene. Fruitful interactions between basic scientists and clinical researchers within medicine and the pharmaceutical industry will, it is hoped, provide for future advances in this area.