The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform

Haolin Chen - One of the best experts on this subject based on the ideXlab platform.

  • tcf21 mesenchymal cells contribute to testis somatic cell development homeostasis and regeneration in mice
    Nature Communications, 2021
    Co-Authors: Yuchi Shen, Meena Sukhwani, Hailey Larose, Adrienne Niederriter Shami, Lindsay Moritz, Gabriel L Manske, Xianing Zheng, Michael Czerwinski, Caleb Sultan, Haolin Chen
    Abstract:

    Testicular development and function rely on interactions between somatic cells and the germline, but similar to other organs, regenerative capacity declines in aging and disease. Whether the adult testis maintains a reserve progenitor population remains uncertain. Here, we characterize a recently identified mouse testis interstitial population expressing the transcription factor Tcf21. We found that TCF21lin cells are bipotential somatic progenitors present in fetal testis and ovary, maintain adult testis homeostasis during aging, and act as potential reserve somatic progenitors following injury. In vitro, TCF21lin cells are multipotent mesenchymal progenitors which form multiple somatic lineages including Leydig and myoid cells. Additionally, TCF21+ cells resemble resident fibroblast populations reported in other organs having roles in tissue homeostasis, fibrosis, and regeneration. Our findings reveal that the testis, like other organs, maintains multipotent mesenchymal progenitors that can be potentially leveraged in development of future therapies for Hypoandrogenism and/or infertility. Whether the adult testis harbours a somatic progenitor population is unknown. Here, the authors provide evidence that the testis interstitial cells expressing the transcription factor Tcf21 maintain adult testis homeostasis during aging, and act as potential reserve somatic progenitors following injury.

Yuchi Shen - One of the best experts on this subject based on the ideXlab platform.

  • tcf21 mesenchymal cells contribute to testis somatic cell development homeostasis and regeneration in mice
    Nature Communications, 2021
    Co-Authors: Yuchi Shen, Meena Sukhwani, Hailey Larose, Adrienne Niederriter Shami, Lindsay Moritz, Gabriel L Manske, Xianing Zheng, Michael Czerwinski, Caleb Sultan, Haolin Chen
    Abstract:

    Testicular development and function rely on interactions between somatic cells and the germline, but similar to other organs, regenerative capacity declines in aging and disease. Whether the adult testis maintains a reserve progenitor population remains uncertain. Here, we characterize a recently identified mouse testis interstitial population expressing the transcription factor Tcf21. We found that TCF21lin cells are bipotential somatic progenitors present in fetal testis and ovary, maintain adult testis homeostasis during aging, and act as potential reserve somatic progenitors following injury. In vitro, TCF21lin cells are multipotent mesenchymal progenitors which form multiple somatic lineages including Leydig and myoid cells. Additionally, TCF21+ cells resemble resident fibroblast populations reported in other organs having roles in tissue homeostasis, fibrosis, and regeneration. Our findings reveal that the testis, like other organs, maintains multipotent mesenchymal progenitors that can be potentially leveraged in development of future therapies for Hypoandrogenism and/or infertility. Whether the adult testis harbours a somatic progenitor population is unknown. Here, the authors provide evidence that the testis interstitial cells expressing the transcription factor Tcf21 maintain adult testis homeostasis during aging, and act as potential reserve somatic progenitors following injury.

  • tcf21 mesenchymal cells contribute to testis somatic cell development homeostasis and regeneration
    bioRxiv, 2020
    Co-Authors: Yuchi Shen, Meena Sukhwani, Hailey Larose, Adrienne Niederriter Shami, Lindsay Moritz, Gabriel L Manske, Xianing Zheng, Michael Czerwinski, Caleb Sultan, Jourdan Clements
    Abstract:

    Testicular development and function relies on interactions between somatic cells and the germline, but similar to other organs, regenerative capacity decline in aging and disease. Whether the adult testis maintains a reserve progenitor population with repair or regenerative capacity remains uncertain. Here, we characterized a recently identified mouse testis interstitial population expressing the transcription factor Tcf21. We found that Tcf21+ cells are bipotential somatic progenitors present in fetal testis and ovary, maintain adult testis homeostasis during aging, and act as reserve somatic progenitors following injury. In vitro, Tcf21+ cells are multipotent mesenchymal progenitors which form multiple somatic lineages including Leydig and myoid cells. Additionally, Tcf21+ cells resemble resident fibroblast populations reported in other organs having roles in tissue homeostasis, fibrosis, and regeneration. Our findings reveal that the testis, like other organs, maintains multipotent mesenchymal progenitors that can be leveraged in development of future therapies for Hypoandrogenism and/or infertility. HighlightsO_LIMultipotent Tcf21+ MPs can differentiate into somatic testis cell types C_LIO_LITcf21+ cells contribute to testis and ovary somatic cells during gonadal development C_LIO_LITcf21+ cells replenish somatic cells of the aging testis and in response to tissue injury C_LIO_LITestis Tcf21 cells resemble resident fibroblast populations in multiple organs C_LI

Jukka T Salonen - One of the best experts on this subject based on the ideXlab platform.

  • CLINICAL STUDY Sex hormones, inflammation and the metabolic syndrome: a population-based study
    2013
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Riitta Salonen, Rainer Rauramaa, Jukka T Salonen
    Abstract:

    Objective: Mild Hypoandrogenism in men is associated with features of the metabolic syndrome, but the association with the metabolic syndrome itself using an accepted definition has not been described. Design: Men with the metabolic syndrome were identified and testosterone and sex hormone-binding globulin (SHBG) levels were determined in a population-based cohort of 1896 non-diabetic middleaged Finnish men. Results: Calculated free testosterone and SHBG were 11 % and 18 % lower (P, 0.001) in men with the metabolic syndrome (n 345, World Health Organisation definition). After categorisation by tertiles and adjusting for age and body mass index, total and free testosterone and SHBG were inversely associated with concentrations of insulin, glucose, triglycerides, C-reactive protein (CRP) and CRP-adjusted ferritin and positively associated with high-density lipoprotein cholesterol. Men with free testosterone levels in the lowest third were 2.7 (95 % confidence interval (CI) 2.0–3.7) times more likely to have the metabolic syndrome in age-adjusted analyses, and 1.7 (95 % CI 1.2 –2.4) times more likely even after further adjusting for body mass index. Exclusion of men with cardiovascula

  • the metabolic syndrome and smoking in relation to hypogonadism in middle aged men a prospective cohort study
    The Journal of Clinical Endocrinology and Metabolism, 2005
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Velipekka Valkonen, Jukka T Salonen
    Abstract:

    In men, Hypoandrogenism is associated with features of the metabolic syndrome. It is not known whether men with the metabolic syndrome are at a higher risk of developing hypogonadism. We therefore assessed whether the metabolic syndrome predicts development of hypogonadism 11 yr later in 651 middle-aged Finnish men participating in a population-based cohort study. Men with the metabolic syndrome at baseline as defined by the World Health Organization (n = 114, 20%) had a 2.6-fold increased risk of developing hypogonadism as defined by total testosterone levels less than 11 nmol/liter at the 11-yr follow-up independent of age, smoking, and other potential confounders. Further adjustment for body mass index (OR, 2.0; 95% CI, 1.1-3.8) or baseline total testosterone levels (OR, 1.9; 95% CI, 1.0-3.4) attenuated the association. The association of the metabolic syndrome with hypogonadism as defined by calculated free testosterone levels less than 225 pmol/liter was similar, but weaker. The adjusted decrease in testosterone concentrations during the 11-yr follow-up was also greater in men with than without the metabolic syndrome. Smokers had a nonsignificantly lower risk of developing hypogonadism during follow-up, whereas a decrease in smoking increased the risk of hypogonadism. The metabolic syndrome predisposes to development of hypogonadism in middle-aged men. Prevention of abdominal obesity and the accompanying metabolic syndrome in middle age may decrease the risk of hypogonadism in men, especially in those who quit smoking.

  • testosterone and sex hormone binding globulin predict the metabolic syndrome and diabetes in middle aged men
    Diabetes Care, 2004
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Velipekka Valkonen, Riitta Salonen, Jukka T Salonen
    Abstract:

    OBJECTIVE —In men, Hypoandrogenism is associated with features of the metabolic syndrome, but the role of sex hormones in the pathogenesis of the metabolic syndrome and diabetes is not well understood. We assessed the association of low levels of testosterone and sex hormone–binding globulin (SHBG) with the development of the metabolic syndrome and diabetes in men. RESEARCH DESIGN AND METHODS —Concentrations of SHBG and total and calculated free testosterone and factors related to insulin resistance were determined at baseline in 702 middle-aged Finnish men participating in a population-based cohort study. These men had neither diabetes nor the metabolic syndrome. RESULTS —After 11 years of follow-up, 147 men had developed the metabolic syndrome (National Cholesterol Education Program criteria) and 57 men diabetes. Men with total testosterone, calculated free testosterone, and SHBG levels in the lower fourth had a severalfold increased risk of developing the metabolic syndrome (odds ratio [OR] 2.3, 95% CI 1.5–3.4; 1.7, 1.2–2.5; and 2.8, 1.9–4.1, respectively) and diabetes (2.3, 1.3–4.1; 1.7, 0.9–3.0; and 4.3, 2.4–7.7, respectively) after adjustment for age. Adjustment for potential confounders such as cardiovascular disease, smoking, alcohol intake, and socioeconomic status did not alter the associations. Factors related to insulin resistance attenuated the associations, but they remained significant, except for free testosterone. CONCLUSIONS —Low total testosterone and SHBG levels independently predict development of the metabolic syndrome and diabetes in middle-aged men. Thus, Hypoandrogenism is an early marker for disturbances in insulin and glucose metabolism that may progress to the metabolic syndrome or frank diabetes and may contribute to their pathogenesis.

  • sex hormones inflammation and the metabolic syndrome a population based study
    European Journal of Endocrinology, 2003
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Riitta Salonen, Rainer Rauramaa, Jukka T Salonen
    Abstract:

    Objective: Mild Hypoandrogenism in men is associated with features of the metabolic syndrome, but the association with the metabolic syndrome itself using an accepted definition has not been described. Design: Men with the metabolic syndrome were identified and testosterone and sex hormone-binding globulin (SHBG) levels were determined in a population-based cohort of 1896 non-diabetic middleaged Finnish men. Results: Calculated free testosterone and SHBG were 11% and 18% lower (P , 0.001) in men with the metabolic syndrome (n ¼ 345, World Health Organisation definition). After categorisation by tertiles and adjusting for age and body mass index, total and free testosterone and SHBG were inversely associated with concentrations of insulin, glucose, triglycerides, C-reactive protein (CRP) and CRP-adjusted ferritin and positively associated with high-density lipoprotein cholesterol. Men with free testosterone levels in the lowest third were 2.7 (95% confidence interval (CI) 2.0 – 3.7) times more likely to have the metabolic syndrome in age-adjusted analyses, and 1.7 (95% CI 1.2 – 2.4) times more likely even after further adjusting for body mass index. Exclusion of men with cardiovascular disease did not alter the association. The inverse association of SHBG with the metabolic syndrome was somewhat stronger. Conclusions: Low testosterone and SHBG levels were strongly associated not only with components of the metabolic syndrome, but also with the metabolic syndrome itself, independently of body mass index. Furthermore, sex hormones were associated with inflammation and body iron stores. Even in the absence of late-stage consequences such as diabetes and cardiovascular disease, subtle derangements in sex hormones are present in the metabolic syndrome, and may contribute to its pathogenesis.

David E Laaksonen - One of the best experts on this subject based on the ideXlab platform.

  • CLINICAL STUDY Sex hormones, inflammation and the metabolic syndrome: a population-based study
    2013
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Riitta Salonen, Rainer Rauramaa, Jukka T Salonen
    Abstract:

    Objective: Mild Hypoandrogenism in men is associated with features of the metabolic syndrome, but the association with the metabolic syndrome itself using an accepted definition has not been described. Design: Men with the metabolic syndrome were identified and testosterone and sex hormone-binding globulin (SHBG) levels were determined in a population-based cohort of 1896 non-diabetic middleaged Finnish men. Results: Calculated free testosterone and SHBG were 11 % and 18 % lower (P, 0.001) in men with the metabolic syndrome (n 345, World Health Organisation definition). After categorisation by tertiles and adjusting for age and body mass index, total and free testosterone and SHBG were inversely associated with concentrations of insulin, glucose, triglycerides, C-reactive protein (CRP) and CRP-adjusted ferritin and positively associated with high-density lipoprotein cholesterol. Men with free testosterone levels in the lowest third were 2.7 (95 % confidence interval (CI) 2.0–3.7) times more likely to have the metabolic syndrome in age-adjusted analyses, and 1.7 (95 % CI 1.2 –2.4) times more likely even after further adjusting for body mass index. Exclusion of men with cardiovascula

  • the metabolic syndrome and smoking in relation to hypogonadism in middle aged men a prospective cohort study
    The Journal of Clinical Endocrinology and Metabolism, 2005
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Velipekka Valkonen, Jukka T Salonen
    Abstract:

    In men, Hypoandrogenism is associated with features of the metabolic syndrome. It is not known whether men with the metabolic syndrome are at a higher risk of developing hypogonadism. We therefore assessed whether the metabolic syndrome predicts development of hypogonadism 11 yr later in 651 middle-aged Finnish men participating in a population-based cohort study. Men with the metabolic syndrome at baseline as defined by the World Health Organization (n = 114, 20%) had a 2.6-fold increased risk of developing hypogonadism as defined by total testosterone levels less than 11 nmol/liter at the 11-yr follow-up independent of age, smoking, and other potential confounders. Further adjustment for body mass index (OR, 2.0; 95% CI, 1.1-3.8) or baseline total testosterone levels (OR, 1.9; 95% CI, 1.0-3.4) attenuated the association. The association of the metabolic syndrome with hypogonadism as defined by calculated free testosterone levels less than 225 pmol/liter was similar, but weaker. The adjusted decrease in testosterone concentrations during the 11-yr follow-up was also greater in men with than without the metabolic syndrome. Smokers had a nonsignificantly lower risk of developing hypogonadism during follow-up, whereas a decrease in smoking increased the risk of hypogonadism. The metabolic syndrome predisposes to development of hypogonadism in middle-aged men. Prevention of abdominal obesity and the accompanying metabolic syndrome in middle age may decrease the risk of hypogonadism in men, especially in those who quit smoking.

  • testosterone and sex hormone binding globulin predict the metabolic syndrome and diabetes in middle aged men
    Diabetes Care, 2004
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Velipekka Valkonen, Riitta Salonen, Jukka T Salonen
    Abstract:

    OBJECTIVE —In men, Hypoandrogenism is associated with features of the metabolic syndrome, but the role of sex hormones in the pathogenesis of the metabolic syndrome and diabetes is not well understood. We assessed the association of low levels of testosterone and sex hormone–binding globulin (SHBG) with the development of the metabolic syndrome and diabetes in men. RESEARCH DESIGN AND METHODS —Concentrations of SHBG and total and calculated free testosterone and factors related to insulin resistance were determined at baseline in 702 middle-aged Finnish men participating in a population-based cohort study. These men had neither diabetes nor the metabolic syndrome. RESULTS —After 11 years of follow-up, 147 men had developed the metabolic syndrome (National Cholesterol Education Program criteria) and 57 men diabetes. Men with total testosterone, calculated free testosterone, and SHBG levels in the lower fourth had a severalfold increased risk of developing the metabolic syndrome (odds ratio [OR] 2.3, 95% CI 1.5–3.4; 1.7, 1.2–2.5; and 2.8, 1.9–4.1, respectively) and diabetes (2.3, 1.3–4.1; 1.7, 0.9–3.0; and 4.3, 2.4–7.7, respectively) after adjustment for age. Adjustment for potential confounders such as cardiovascular disease, smoking, alcohol intake, and socioeconomic status did not alter the associations. Factors related to insulin resistance attenuated the associations, but they remained significant, except for free testosterone. CONCLUSIONS —Low total testosterone and SHBG levels independently predict development of the metabolic syndrome and diabetes in middle-aged men. Thus, Hypoandrogenism is an early marker for disturbances in insulin and glucose metabolism that may progress to the metabolic syndrome or frank diabetes and may contribute to their pathogenesis.

  • changes in sex hormone binding globulin and testosterone during weight loss and weight maintenance in abdominally obese men with the metabolic syndrome
    Diabetes Obesity and Metabolism, 2004
    Co-Authors: L Niskanen, David E Laaksonen, Kari Punnonen, Pertti Mustajoki, J Kaukua, Aila Rissanen
    Abstract:

    Background:  Mild Hypoandrogenism in men, usually defined by low levels of testosterone, is a peculiar feature of abdominal obesity that independently predicts the development of insulin resistance and diabetes mellitus. Little is known about the short- and long-term effects of weight loss on sex steroids in abdominally obese men, however. Objectives:  We assessed the effect of rapid weight loss and sustained weight maintenance on the plasma concentrations of testosterone and other sex hormones in 58 abdominally obese men (age, 46.3 ± 7.5 years; body mass index, 36.1 ± 3.8 kg/m2; waist girth, 121 ± 10 cm) with the metabolic syndrome. Results:  The men lost on average 16.3 ± 4.5 kg during a 9-week very low-calorie diet (VLCD) and maintained 14.3 ± 9.1 kg weight loss after a 12-month maintenance period (vs. baseline, p < 0.001). Sex hormone-binding globulin (SHBG) increased from 27.6 ± 11.9 to 48.1 ± 23.5 nmol/l during the VLCD but decreased to 32.6 ± 12.9 nmol/l during weight maintenance, which was still higher than at baseline (p < 0.001). Free testosterone (fT) increased from 185 ± 66 to 208 ± 70 pmol/l (p = 0.002) during the VLCD and remained high after 1 year of weight maintenance (212 ± 84 pmol/l, p = 0.002). Total testosterone levels followed a pattern intermediate between fT and SHBG. Plasma estradiol and dehydroepiandrosterone sulphate concentrations changed only transiently or not at all. Conclusions:  Rapid weight loss with successful weight maintenance in abdominally obese men with the metabolic syndrome brings about a sustained increase in fT levels. The dramatic increase in SHBG attenuated initially during weight maintenance but remained elevated. These findings may be important with regard to prevention of progressive metabolic decompensation and cardiovascular disease associated with obesity and the metabolic syndrome.

  • sex hormones inflammation and the metabolic syndrome a population based study
    European Journal of Endocrinology, 2003
    Co-Authors: David E Laaksonen, Leo Niskanen, Kari Punnonen, Kristiina Nyyssonen, Tomipekka Tuomainen, Riitta Salonen, Rainer Rauramaa, Jukka T Salonen
    Abstract:

    Objective: Mild Hypoandrogenism in men is associated with features of the metabolic syndrome, but the association with the metabolic syndrome itself using an accepted definition has not been described. Design: Men with the metabolic syndrome were identified and testosterone and sex hormone-binding globulin (SHBG) levels were determined in a population-based cohort of 1896 non-diabetic middleaged Finnish men. Results: Calculated free testosterone and SHBG were 11% and 18% lower (P , 0.001) in men with the metabolic syndrome (n ¼ 345, World Health Organisation definition). After categorisation by tertiles and adjusting for age and body mass index, total and free testosterone and SHBG were inversely associated with concentrations of insulin, glucose, triglycerides, C-reactive protein (CRP) and CRP-adjusted ferritin and positively associated with high-density lipoprotein cholesterol. Men with free testosterone levels in the lowest third were 2.7 (95% confidence interval (CI) 2.0 – 3.7) times more likely to have the metabolic syndrome in age-adjusted analyses, and 1.7 (95% CI 1.2 – 2.4) times more likely even after further adjusting for body mass index. Exclusion of men with cardiovascular disease did not alter the association. The inverse association of SHBG with the metabolic syndrome was somewhat stronger. Conclusions: Low testosterone and SHBG levels were strongly associated not only with components of the metabolic syndrome, but also with the metabolic syndrome itself, independently of body mass index. Furthermore, sex hormones were associated with inflammation and body iron stores. Even in the absence of late-stage consequences such as diabetes and cardiovascular disease, subtle derangements in sex hormones are present in the metabolic syndrome, and may contribute to its pathogenesis.

Meena Sukhwani - One of the best experts on this subject based on the ideXlab platform.

  • tcf21 mesenchymal cells contribute to testis somatic cell development homeostasis and regeneration in mice
    Nature Communications, 2021
    Co-Authors: Yuchi Shen, Meena Sukhwani, Hailey Larose, Adrienne Niederriter Shami, Lindsay Moritz, Gabriel L Manske, Xianing Zheng, Michael Czerwinski, Caleb Sultan, Haolin Chen
    Abstract:

    Testicular development and function rely on interactions between somatic cells and the germline, but similar to other organs, regenerative capacity declines in aging and disease. Whether the adult testis maintains a reserve progenitor population remains uncertain. Here, we characterize a recently identified mouse testis interstitial population expressing the transcription factor Tcf21. We found that TCF21lin cells are bipotential somatic progenitors present in fetal testis and ovary, maintain adult testis homeostasis during aging, and act as potential reserve somatic progenitors following injury. In vitro, TCF21lin cells are multipotent mesenchymal progenitors which form multiple somatic lineages including Leydig and myoid cells. Additionally, TCF21+ cells resemble resident fibroblast populations reported in other organs having roles in tissue homeostasis, fibrosis, and regeneration. Our findings reveal that the testis, like other organs, maintains multipotent mesenchymal progenitors that can be potentially leveraged in development of future therapies for Hypoandrogenism and/or infertility. Whether the adult testis harbours a somatic progenitor population is unknown. Here, the authors provide evidence that the testis interstitial cells expressing the transcription factor Tcf21 maintain adult testis homeostasis during aging, and act as potential reserve somatic progenitors following injury.

  • tcf21 mesenchymal cells contribute to testis somatic cell development homeostasis and regeneration
    bioRxiv, 2020
    Co-Authors: Yuchi Shen, Meena Sukhwani, Hailey Larose, Adrienne Niederriter Shami, Lindsay Moritz, Gabriel L Manske, Xianing Zheng, Michael Czerwinski, Caleb Sultan, Jourdan Clements
    Abstract:

    Testicular development and function relies on interactions between somatic cells and the germline, but similar to other organs, regenerative capacity decline in aging and disease. Whether the adult testis maintains a reserve progenitor population with repair or regenerative capacity remains uncertain. Here, we characterized a recently identified mouse testis interstitial population expressing the transcription factor Tcf21. We found that Tcf21+ cells are bipotential somatic progenitors present in fetal testis and ovary, maintain adult testis homeostasis during aging, and act as reserve somatic progenitors following injury. In vitro, Tcf21+ cells are multipotent mesenchymal progenitors which form multiple somatic lineages including Leydig and myoid cells. Additionally, Tcf21+ cells resemble resident fibroblast populations reported in other organs having roles in tissue homeostasis, fibrosis, and regeneration. Our findings reveal that the testis, like other organs, maintains multipotent mesenchymal progenitors that can be leveraged in development of future therapies for Hypoandrogenism and/or infertility. HighlightsO_LIMultipotent Tcf21+ MPs can differentiate into somatic testis cell types C_LIO_LITcf21+ cells contribute to testis and ovary somatic cells during gonadal development C_LIO_LITcf21+ cells replenish somatic cells of the aging testis and in response to tissue injury C_LIO_LITestis Tcf21 cells resemble resident fibroblast populations in multiple organs C_LI