The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
Yasushi Nakagawa - One of the best experts on this subject based on the ideXlab platform.
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Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei
Neural development, 2016Co-Authors: Katie Sokolowski, Tuyen Tran, Shigeyuki Esumi, Yasmin Kamal, Livio Oboti, Julieta E. Lischinsky, Meredith Goodrich, Andrew Lam, Margaret Carter, Yasushi NakagawaAbstract:Background Neurons in the Hypothalamus Function to regulate the state of the animal during both learned and innate behaviors, and alterations in hypothalamic development may contribute to pathological conditions such as anxiety, depression or obesity. Despite many studies of hypothalamic development and Function, the link between embryonic development and innate behaviors remains unexplored. Here, focusing on the embryonically expressed homeodomain-containing gene Developing Brain Homeobox 1 (Dbx1), we explored the relationship between embryonic lineage, post-natal neuronal identity and lineage-specific responses to innate cues. We found that Dbx1 is widely expressed across multiple developing hypothalamic subdomains. Using standard and inducible fate-mapping to trace the Dbx1-derived neurons, we identified their contribution to specific neuronal subtypes across hypothalamic nuclei and further mapped their activation patterns in response to a series of well-defined innate behaviors.
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Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei
Neural Development, 2016Co-Authors: Katie Sokolowski, Tuyen Tran, Shigeyuki Esumi, Yasmin Kamal, Livio Oboti, Julieta E. Lischinsky, Meredith Goodrich, Andrew Lam, Margaret Carter, Yasushi NakagawaAbstract:Background Neurons in the Hypothalamus Function to regulate the state of the animal during both learned and innate behaviors, and alterations in hypothalamic development may contribute to pathological conditions such as anxiety, depression or obesity. Despite many studies of hypothalamic development and Function, the link between embryonic development and innate behaviors remains unexplored. Here, focusing on the embryonically expressed homeodomain-containing gene Developing Brain Homeobox 1 ( Dbx1 ), we explored the relationship between embryonic lineage, post-natal neuronal identity and lineage-specific responses to innate cues. We found that Dbx1 is widely expressed across multiple developing hypothalamic subdomains. Using standard and inducible fate-mapping to trace the Dbx1 -derived neurons, we identified their contribution to specific neuronal subtypes across hypothalamic nuclei and further mapped their activation patterns in response to a series of well-defined innate behaviors. Results Dbx1 -derived neurons occupy multiple postnatal hypothalamic nuclei including the lateral Hypothalamus (LH), arcuate nucleus (Arc) and the ventral medial Hypothalamus (VMH). Within these nuclei, Dbx1 ^+ progenitors generate a large proportion of the Pmch-, Nesfatin-, Cart-, Hcrt-, Agrp- and ERα-expressing neuronal populations, and to a lesser extent the Pomc-, TH- and Aromatase-expressing populations. Inducible fate-mapping reveals distinct temporal windows for development of the Dbx1 -derived LH and Arc populations, with Agrp^+ and Cart^+ populations in the Arc arising early (E7.5-E9.5), while Pmch^+ and Hcrt^+ populations in the LH derived from progenitors expressing Dbx1 later (E9.5-E11.5). Moreover, as revealed by c-Fos labeling, Dbx1 -derived cells in male and female LH, Arc and VMH are responsive during mating and aggression. In contrast, Dbx1 -lineage cells in the Arc and LH have a broader behavioral tuning, which includes responding to fasting and predator odor cues. Conclusion We define a novel fate map of the Hypothalamus with respect to Dbx1 expression in hypothalamic progenitor zones. We demonstrate that in a temporally regulated manner, Dbx1 -derived neurons contribute to molecularly distinct neuronal populations in the LH, Arc and VMH that have been implicated in a variety of hypothalamic-driven behaviors. Consistent with this, Dbx1 -derived neurons in the LH, Arc and VMH are activated during stress and other innate behavioral responses, implicating their involvement in these diverse behaviors.
Yasuhiko Minokoshi - One of the best experts on this subject based on the ideXlab platform.
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Hypothalamic neuronal circuits regulating hunger-induced taste modification
Nature communications, 2019Co-Authors: Yuu Iwai, Masataka Narukawa, Ayako Ishikawa, Kentaro Ishii, Ken Murata, Yumiko Yoshimura, Kazushige Touhara, Takumi Misaka, Yasuhiko MinokoshiAbstract:The gustatory system plays a critical role in sensing appetitive and aversive taste stimuli for evaluating food quality. Although taste preference is known to change depending on internal states such as hunger, a mechanistic insight remains unclear. Here, we examine the neuronal mechanisms regulating hunger-induced taste modification. Starved mice exhibit an increased preference for sweetness and tolerance for aversive taste. This hunger-induced taste modification is recapitulated by selective activation of orexigenic Agouti-related peptide (AgRP)-expressing neurons in the Hypothalamus projecting to the lateral Hypothalamus, but not to other regions. Glutamatergic, but not GABAergic, neurons in the lateral Hypothalamus Function as downstream neurons of AgRP neurons. Importantly, these neurons play a key role in modulating preferences for both appetitive and aversive tastes by using distinct pathways projecting to the lateral septum or the lateral habenula, respectively. Our results suggest that these hypothalamic circuits would be important for optimizing feeding behavior under fasting.
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Hypothalamic neuronal circuits regulating hunger-induced taste modification
Nature Communications, 2019Co-Authors: Yuu Iwai, Masataka Narukawa, Ken Murata, Yumiko Yoshimura, Kazushige Touhara, Takumi Misaka, Yasuhiko Minokoshi, Ayako W. Ishikawa, Kentaro K. Ishii, Ken-ichiro NakajimaAbstract:Hunger modulates perception of good and bad tastes. Here, the authors report that orexigenic AgRP neurons in the Hypothalamus mediate these effects through glutamatergic lateral hypothalamic neurons that send distinct projections to the lateral septum and lateral habenula. The gustatory system plays a critical role in sensing appetitive and aversive taste stimuli for evaluating food quality. Although taste preference is known to change depending on internal states such as hunger, a mechanistic insight remains unclear. Here, we examine the neuronal mechanisms regulating hunger-induced taste modification. Starved mice exhibit an increased preference for sweetness and tolerance for aversive taste. This hunger-induced taste modification is recapitulated by selective activation of orexigenic Agouti-related peptide (AgRP)-expressing neurons in the Hypothalamus projecting to the lateral Hypothalamus, but not to other regions. Glutamatergic, but not GABAergic, neurons in the lateral Hypothalamus Function as downstream neurons of AgRP neurons. Importantly, these neurons play a key role in modulating preferences for both appetitive and aversive tastes by using distinct pathways projecting to the lateral septum or the lateral habenula, respectively. Our results suggest that these hypothalamic circuits would be important for optimizing feeding behavior under fasting.
Yuu Iwai - One of the best experts on this subject based on the ideXlab platform.
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Hypothalamic neuronal circuits regulating hunger-induced taste modification
Nature communications, 2019Co-Authors: Yuu Iwai, Masataka Narukawa, Ayako Ishikawa, Kentaro Ishii, Ken Murata, Yumiko Yoshimura, Kazushige Touhara, Takumi Misaka, Yasuhiko MinokoshiAbstract:The gustatory system plays a critical role in sensing appetitive and aversive taste stimuli for evaluating food quality. Although taste preference is known to change depending on internal states such as hunger, a mechanistic insight remains unclear. Here, we examine the neuronal mechanisms regulating hunger-induced taste modification. Starved mice exhibit an increased preference for sweetness and tolerance for aversive taste. This hunger-induced taste modification is recapitulated by selective activation of orexigenic Agouti-related peptide (AgRP)-expressing neurons in the Hypothalamus projecting to the lateral Hypothalamus, but not to other regions. Glutamatergic, but not GABAergic, neurons in the lateral Hypothalamus Function as downstream neurons of AgRP neurons. Importantly, these neurons play a key role in modulating preferences for both appetitive and aversive tastes by using distinct pathways projecting to the lateral septum or the lateral habenula, respectively. Our results suggest that these hypothalamic circuits would be important for optimizing feeding behavior under fasting.
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Hypothalamic neuronal circuits regulating hunger-induced taste modification
Nature Communications, 2019Co-Authors: Yuu Iwai, Masataka Narukawa, Ken Murata, Yumiko Yoshimura, Kazushige Touhara, Takumi Misaka, Yasuhiko Minokoshi, Ayako W. Ishikawa, Kentaro K. Ishii, Ken-ichiro NakajimaAbstract:Hunger modulates perception of good and bad tastes. Here, the authors report that orexigenic AgRP neurons in the Hypothalamus mediate these effects through glutamatergic lateral hypothalamic neurons that send distinct projections to the lateral septum and lateral habenula. The gustatory system plays a critical role in sensing appetitive and aversive taste stimuli for evaluating food quality. Although taste preference is known to change depending on internal states such as hunger, a mechanistic insight remains unclear. Here, we examine the neuronal mechanisms regulating hunger-induced taste modification. Starved mice exhibit an increased preference for sweetness and tolerance for aversive taste. This hunger-induced taste modification is recapitulated by selective activation of orexigenic Agouti-related peptide (AgRP)-expressing neurons in the Hypothalamus projecting to the lateral Hypothalamus, but not to other regions. Glutamatergic, but not GABAergic, neurons in the lateral Hypothalamus Function as downstream neurons of AgRP neurons. Importantly, these neurons play a key role in modulating preferences for both appetitive and aversive tastes by using distinct pathways projecting to the lateral septum or the lateral habenula, respectively. Our results suggest that these hypothalamic circuits would be important for optimizing feeding behavior under fasting.
Katie Sokolowski - One of the best experts on this subject based on the ideXlab platform.
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Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei
Neural development, 2016Co-Authors: Katie Sokolowski, Tuyen Tran, Shigeyuki Esumi, Yasmin Kamal, Livio Oboti, Julieta E. Lischinsky, Meredith Goodrich, Andrew Lam, Margaret Carter, Yasushi NakagawaAbstract:Background Neurons in the Hypothalamus Function to regulate the state of the animal during both learned and innate behaviors, and alterations in hypothalamic development may contribute to pathological conditions such as anxiety, depression or obesity. Despite many studies of hypothalamic development and Function, the link between embryonic development and innate behaviors remains unexplored. Here, focusing on the embryonically expressed homeodomain-containing gene Developing Brain Homeobox 1 (Dbx1), we explored the relationship between embryonic lineage, post-natal neuronal identity and lineage-specific responses to innate cues. We found that Dbx1 is widely expressed across multiple developing hypothalamic subdomains. Using standard and inducible fate-mapping to trace the Dbx1-derived neurons, we identified their contribution to specific neuronal subtypes across hypothalamic nuclei and further mapped their activation patterns in response to a series of well-defined innate behaviors.
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Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei
Neural Development, 2016Co-Authors: Katie Sokolowski, Tuyen Tran, Shigeyuki Esumi, Yasmin Kamal, Livio Oboti, Julieta E. Lischinsky, Meredith Goodrich, Andrew Lam, Margaret Carter, Yasushi NakagawaAbstract:Background Neurons in the Hypothalamus Function to regulate the state of the animal during both learned and innate behaviors, and alterations in hypothalamic development may contribute to pathological conditions such as anxiety, depression or obesity. Despite many studies of hypothalamic development and Function, the link between embryonic development and innate behaviors remains unexplored. Here, focusing on the embryonically expressed homeodomain-containing gene Developing Brain Homeobox 1 ( Dbx1 ), we explored the relationship between embryonic lineage, post-natal neuronal identity and lineage-specific responses to innate cues. We found that Dbx1 is widely expressed across multiple developing hypothalamic subdomains. Using standard and inducible fate-mapping to trace the Dbx1 -derived neurons, we identified their contribution to specific neuronal subtypes across hypothalamic nuclei and further mapped their activation patterns in response to a series of well-defined innate behaviors. Results Dbx1 -derived neurons occupy multiple postnatal hypothalamic nuclei including the lateral Hypothalamus (LH), arcuate nucleus (Arc) and the ventral medial Hypothalamus (VMH). Within these nuclei, Dbx1 ^+ progenitors generate a large proportion of the Pmch-, Nesfatin-, Cart-, Hcrt-, Agrp- and ERα-expressing neuronal populations, and to a lesser extent the Pomc-, TH- and Aromatase-expressing populations. Inducible fate-mapping reveals distinct temporal windows for development of the Dbx1 -derived LH and Arc populations, with Agrp^+ and Cart^+ populations in the Arc arising early (E7.5-E9.5), while Pmch^+ and Hcrt^+ populations in the LH derived from progenitors expressing Dbx1 later (E9.5-E11.5). Moreover, as revealed by c-Fos labeling, Dbx1 -derived cells in male and female LH, Arc and VMH are responsive during mating and aggression. In contrast, Dbx1 -lineage cells in the Arc and LH have a broader behavioral tuning, which includes responding to fasting and predator odor cues. Conclusion We define a novel fate map of the Hypothalamus with respect to Dbx1 expression in hypothalamic progenitor zones. We demonstrate that in a temporally regulated manner, Dbx1 -derived neurons contribute to molecularly distinct neuronal populations in the LH, Arc and VMH that have been implicated in a variety of hypothalamic-driven behaviors. Consistent with this, Dbx1 -derived neurons in the LH, Arc and VMH are activated during stress and other innate behavioral responses, implicating their involvement in these diverse behaviors.
Ken-ichiro Nakajima - One of the best experts on this subject based on the ideXlab platform.
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Hypothalamic neuronal circuits regulating hunger-induced taste modification
Nature Communications, 2019Co-Authors: Yuu Iwai, Masataka Narukawa, Ken Murata, Yumiko Yoshimura, Kazushige Touhara, Takumi Misaka, Yasuhiko Minokoshi, Ayako W. Ishikawa, Kentaro K. Ishii, Ken-ichiro NakajimaAbstract:Hunger modulates perception of good and bad tastes. Here, the authors report that orexigenic AgRP neurons in the Hypothalamus mediate these effects through glutamatergic lateral hypothalamic neurons that send distinct projections to the lateral septum and lateral habenula. The gustatory system plays a critical role in sensing appetitive and aversive taste stimuli for evaluating food quality. Although taste preference is known to change depending on internal states such as hunger, a mechanistic insight remains unclear. Here, we examine the neuronal mechanisms regulating hunger-induced taste modification. Starved mice exhibit an increased preference for sweetness and tolerance for aversive taste. This hunger-induced taste modification is recapitulated by selective activation of orexigenic Agouti-related peptide (AgRP)-expressing neurons in the Hypothalamus projecting to the lateral Hypothalamus, but not to other regions. Glutamatergic, but not GABAergic, neurons in the lateral Hypothalamus Function as downstream neurons of AgRP neurons. Importantly, these neurons play a key role in modulating preferences for both appetitive and aversive tastes by using distinct pathways projecting to the lateral septum or the lateral habenula, respectively. Our results suggest that these hypothalamic circuits would be important for optimizing feeding behavior under fasting.