The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
S.s. Garber - One of the best experts on this subject based on the ideXlab platform.
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Monitoring Cl^− Movement in Single Cells Exposed to Hypotonic Solution
The Journal of Membrane Biology, 2005Co-Authors: S.s. Garber, M.a. Messerli, M. Hubert, R. Lewis, K. Hammar, E. Indyk, P.j.s. SmithAbstract:Self-referencing ion - selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1–3 μm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparing potentials ( V _N) measured in 100 and 10 m M NaCl (Δ V _N = 57 ± 2 mV), but was slightly greater than ideal when comparing 1 and 10 m m NaCl (Δ V _N = 70 ± 3 mV). The response was also linear in the presence of 1 m m glutamate, gluconate, or acetate, 10 μ m tamoxifen, or 0.1, 1, or 10 m m HEPES at pH 7.0. The ISE was ∼3 orders of magnitude more selective for Cl^− over glutamate or gluconate but less than 2 orders of magnitude move selective for Cl^− over bicarbonate, acetate, citrate or thiosulfate. As a result this ISE is best described as an anion sensor. The ISE was ‘poisoned’ by 50 μ m 5−nitro-2-(3phenylpropyl-amino)-benzoic acid (NPPB), but not by tamoxifen. An outward anion efflux was recorded from cells challenged with Hypotonic (250 ± 5 mOsm) Solution. The increase in efflux peaked 7–8 min before decreasing, consistent with regulatory volume decreases observed in separate experiments using a similar osmotic protocol. This anion efflux was blocked by 10 μ m tamoxifen. These results establish the feasibility of using the modulation of electrochemical, anion-selective, electrodes to monitor anions and, in this case, chloride movement during volume regulatory events. The approach provides a real-time measure of anion movement during regulated volume decrease at the single-cell level.
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monitoring cl movement in single cells exposed to Hypotonic Solution
The Journal of Membrane Biology, 2005Co-Authors: S.s. Garber, M.a. Messerli, M. Hubert, R. Lewis, K. Hammar, E. Indyk, Pete SmithAbstract:Self-referencing ion - selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1–3 μm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparing potentials (VN) measured in 100 and 10 mM NaCl (ΔVN = 57 ± 2 mV), but was slightly greater than ideal when comparing 1 and 10 mm NaCl (ΔVN = 70 ± 3 mV). The response was also linear in the presence of 1 mm glutamate, gluconate, or acetate, 10 μm tamoxifen, or 0.1, 1, or 10 mm HEPES at pH 7.0. The ISE was ∼3 orders of magnitude more selective for Cl− over glutamate or gluconate but less than 2 orders of magnitude move selective for Cl− over bicarbonate, acetate, citrate or thiosulfate. As a result this ISE is best described as an anion sensor. The ISE was ‘poisoned’ by 50 μm 5−nitro-2-(3phenylpropyl-amino)-benzoic acid (NPPB), but not by tamoxifen. An outward anion efflux was recorded from cells challenged with Hypotonic (250 ± 5 mOsm) Solution. The increase in efflux peaked 7–8 min before decreasing, consistent with regulatory volume decreases observed in separate experiments using a similar osmotic protocol. This anion efflux was blocked by 10 μm tamoxifen. These results establish the feasibility of using the modulation of electrochemical, anion-selective, electrodes to monitor anions and, in this case, chloride movement during volume regulatory events. The approach provides a real-time measure of anion movement during regulated volume decrease at the single-cell level.
Min Suk Chae - One of the best experts on this subject based on the ideXlab platform.
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Effect of intraoperative Hartmann’s versus Hypotonic Solution administration on FLACC pain scale scores in children: A prospective randomized controlled trial
PLOS ONE, 2020Co-Authors: Sungwon Yang, Min Suk ChaeAbstract:Background In healthy children, an isotonic Solution containing no glucose or a small amount of glucose (1–2%) has been recommended as an intraoperative maintenance fluid due to the potential risk of hyponatremia associated with Hypotonic Solutions. However, a Hypotonic Solution with glucose is still widely used as a maintenance fluid for pediatric anesthesia. We speculated that the Hypotonic Solution may worsen postoperative discomfort and irritability in pediatric patients due to hyponatremia. Patients and methods In the current study, we compared the post-operative Face, Legs, Activity, Cry, Consolability(FLACC) scale scores of pediatric patients aged 3–10 years who received either a 1:2 dextrose Solution or Hartmann's Solution during Nuss Bar removal. Results The FLACC scale score in the post-anesthesia care unit was higher in the 1:2 dextrose Solution group(HYPO) (n = 20) than in the Hartmann’s Solution group(ISO) (n = 20) (6.30 vs 4.70, p = 0.044, mean difference and 95% Confidence Interval(CI) was 1.6 (0.04 to 3.16)). We also compared opioid consumption at the post-anesthesia care unit. Total dose of fentanyl per body weight in the post-anesthesia care unit was also higher in the HYPO (0.59 vs 0.37 mcg/kg, p = 0.042, mean difference and 95% CI was 0.22 mcg/kg (0.030 to 0.402)). Conclusions Intraoperative use of the Hypotonic Solution in children causes increased FLACC scale scores, leading to higher opioid consumption in post-anesthesia care unit.
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effect of intraoperative hartmann s versus Hypotonic Solution administration on flacc pain scale scores in children a prospective randomized controlled trial
PLOS ONE, 2020Co-Authors: Sungwon Yang, Min Suk ChaeAbstract:Background In healthy children, an isotonic Solution containing no glucose or a small amount of glucose (1–2%) has been recommended as an intraoperative maintenance fluid due to the potential risk of hyponatremia associated with Hypotonic Solutions. However, a Hypotonic Solution with glucose is still widely used as a maintenance fluid for pediatric anesthesia. We speculated that the Hypotonic Solution may worsen postoperative discomfort and irritability in pediatric patients due to hyponatremia. Patients and methods In the current study, we compared the post-operative Face, Legs, Activity, Cry, Consolability(FLACC) scale scores of pediatric patients aged 3–10 years who received either a 1:2 dextrose Solution or Hartmann's Solution during Nuss Bar removal. Results The FLACC scale score in the post-anesthesia care unit was higher in the 1:2 dextrose Solution group(HYPO) (n = 20) than in the Hartmann’s Solution group(ISO) (n = 20) (6.30 vs 4.70, p = 0.044, mean difference and 95% Confidence Interval(CI) was 1.6 (0.04 to 3.16)). We also compared opioid consumption at the post-anesthesia care unit. Total dose of fentanyl per body weight in the post-anesthesia care unit was also higher in the HYPO (0.59 vs 0.37 mcg/kg, p = 0.042, mean difference and 95% CI was 0.22 mcg/kg (0.030 to 0.402)). Conclusions Intraoperative use of the Hypotonic Solution in children causes increased FLACC scale scores, leading to higher opioid consumption in post-anesthesia care unit.
Romina Nassini - One of the best experts on this subject based on the ideXlab platform.
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ureteral relaxation through calcitonin gene related peptide release from sensory nerve terminals by Hypotonic Solution
International Journal of Urology, 2015Co-Authors: Serena Materazzi, Daiana Minocci, Gaetano De Siena, Silvia Benemei, Romina NassiniAbstract:Objectives To evaluate the influence of Hypotonic Solutions on ureteral relaxation mediated by the release of calcitonin gene-related peptide from intramural sensory nerve endings. Methods Urine osmolarity of Sprague–Dawley rats drinking water low in salt content (Fiuggi water) or a reference water for 7 days was measured. Release of calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter and urinary bladder by Hypotonic Solutions was assessed by an immunometric assay. The mechanism through which Hypotonic Solutions inhibit neurokinin A-induced phasic contractions of isolated rat ureters was evaluated by organ bath studies. Results A 7-day consumption of Fiuggi water in rats reduced urine osmolarity by ~40%. Exposure to Hypotonic Solutions released calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter. This response was abated in a calcium-free medium, after capsaicin desensitization, and in the presence of the unselective transient receptor potential channel antagonist, ruthenium red. Exposure of isolated rat ureteral preparations to a Hypotonic Solution inhibited neurokinin A-evoked phasic contraction. This response was attenuated by capsaicin desensitization and in the presence of the calcitonin gene-related peptide receptor antagonist, calcitonin gene-related peptide8-37. Transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4 antagonists did not affect the neurogenic and calcitonin gene-related peptide-dependent relaxation. Conclusion Present data show that Hypotonic Solution evokes calcitonin gene-related peptide release from capsaicin-sensitive intramural sensory nerves, thus inhibiting ureteral contractility, through a transient receptor potential-dependent mechanism. However, this mechanism does not involve transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4. Future studies with appropriate in vivo models should investigate the hypothesis that hypostenuric urine diffusing into the ureteral tissue might favor ureteral relaxation through this novel mechanism.
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Ureteral relaxation through calcitonin gene‐related peptide release from sensory nerve terminals by Hypotonic Solution
International Journal of Urology, 2015Co-Authors: Serena Materazzi, Daiana Minocci, Gaetano De Siena, Silvia Benemei, Romina NassiniAbstract:Objectives To evaluate the influence of Hypotonic Solutions on ureteral relaxation mediated by the release of calcitonin gene-related peptide from intramural sensory nerve endings. Methods Urine osmolarity of Sprague–Dawley rats drinking water low in salt content (Fiuggi water) or a reference water for 7 days was measured. Release of calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter and urinary bladder by Hypotonic Solutions was assessed by an immunometric assay. The mechanism through which Hypotonic Solutions inhibit neurokinin A-induced phasic contractions of isolated rat ureters was evaluated by organ bath studies. Results A 7-day consumption of Fiuggi water in rats reduced urine osmolarity by ~40%. Exposure to Hypotonic Solutions released calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter. This response was abated in a calcium-free medium, after capsaicin desensitization, and in the presence of the unselective transient receptor potential channel antagonist, ruthenium red. Exposure of isolated rat ureteral preparations to a Hypotonic Solution inhibited neurokinin A-evoked phasic contraction. This response was attenuated by capsaicin desensitization and in the presence of the calcitonin gene-related peptide receptor antagonist, calcitonin gene-related peptide8-37. Transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4 antagonists did not affect the neurogenic and calcitonin gene-related peptide-dependent relaxation. Conclusion Present data show that Hypotonic Solution evokes calcitonin gene-related peptide release from capsaicin-sensitive intramural sensory nerves, thus inhibiting ureteral contractility, through a transient receptor potential-dependent mechanism. However, this mechanism does not involve transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4. Future studies with appropriate in vivo models should investigate the hypothesis that hypostenuric urine diffusing into the ureteral tissue might favor ureteral relaxation through this novel mechanism.
Sungwon Yang - One of the best experts on this subject based on the ideXlab platform.
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Effect of intraoperative Hartmann’s versus Hypotonic Solution administration on FLACC pain scale scores in children: A prospective randomized controlled trial
PLOS ONE, 2020Co-Authors: Sungwon Yang, Min Suk ChaeAbstract:Background In healthy children, an isotonic Solution containing no glucose or a small amount of glucose (1–2%) has been recommended as an intraoperative maintenance fluid due to the potential risk of hyponatremia associated with Hypotonic Solutions. However, a Hypotonic Solution with glucose is still widely used as a maintenance fluid for pediatric anesthesia. We speculated that the Hypotonic Solution may worsen postoperative discomfort and irritability in pediatric patients due to hyponatremia. Patients and methods In the current study, we compared the post-operative Face, Legs, Activity, Cry, Consolability(FLACC) scale scores of pediatric patients aged 3–10 years who received either a 1:2 dextrose Solution or Hartmann's Solution during Nuss Bar removal. Results The FLACC scale score in the post-anesthesia care unit was higher in the 1:2 dextrose Solution group(HYPO) (n = 20) than in the Hartmann’s Solution group(ISO) (n = 20) (6.30 vs 4.70, p = 0.044, mean difference and 95% Confidence Interval(CI) was 1.6 (0.04 to 3.16)). We also compared opioid consumption at the post-anesthesia care unit. Total dose of fentanyl per body weight in the post-anesthesia care unit was also higher in the HYPO (0.59 vs 0.37 mcg/kg, p = 0.042, mean difference and 95% CI was 0.22 mcg/kg (0.030 to 0.402)). Conclusions Intraoperative use of the Hypotonic Solution in children causes increased FLACC scale scores, leading to higher opioid consumption in post-anesthesia care unit.
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effect of intraoperative hartmann s versus Hypotonic Solution administration on flacc pain scale scores in children a prospective randomized controlled trial
PLOS ONE, 2020Co-Authors: Sungwon Yang, Min Suk ChaeAbstract:Background In healthy children, an isotonic Solution containing no glucose or a small amount of glucose (1–2%) has been recommended as an intraoperative maintenance fluid due to the potential risk of hyponatremia associated with Hypotonic Solutions. However, a Hypotonic Solution with glucose is still widely used as a maintenance fluid for pediatric anesthesia. We speculated that the Hypotonic Solution may worsen postoperative discomfort and irritability in pediatric patients due to hyponatremia. Patients and methods In the current study, we compared the post-operative Face, Legs, Activity, Cry, Consolability(FLACC) scale scores of pediatric patients aged 3–10 years who received either a 1:2 dextrose Solution or Hartmann's Solution during Nuss Bar removal. Results The FLACC scale score in the post-anesthesia care unit was higher in the 1:2 dextrose Solution group(HYPO) (n = 20) than in the Hartmann’s Solution group(ISO) (n = 20) (6.30 vs 4.70, p = 0.044, mean difference and 95% Confidence Interval(CI) was 1.6 (0.04 to 3.16)). We also compared opioid consumption at the post-anesthesia care unit. Total dose of fentanyl per body weight in the post-anesthesia care unit was also higher in the HYPO (0.59 vs 0.37 mcg/kg, p = 0.042, mean difference and 95% CI was 0.22 mcg/kg (0.030 to 0.402)). Conclusions Intraoperative use of the Hypotonic Solution in children causes increased FLACC scale scores, leading to higher opioid consumption in post-anesthesia care unit.
Serena Materazzi - One of the best experts on this subject based on the ideXlab platform.
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ureteral relaxation through calcitonin gene related peptide release from sensory nerve terminals by Hypotonic Solution
International Journal of Urology, 2015Co-Authors: Serena Materazzi, Daiana Minocci, Gaetano De Siena, Silvia Benemei, Romina NassiniAbstract:Objectives To evaluate the influence of Hypotonic Solutions on ureteral relaxation mediated by the release of calcitonin gene-related peptide from intramural sensory nerve endings. Methods Urine osmolarity of Sprague–Dawley rats drinking water low in salt content (Fiuggi water) or a reference water for 7 days was measured. Release of calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter and urinary bladder by Hypotonic Solutions was assessed by an immunometric assay. The mechanism through which Hypotonic Solutions inhibit neurokinin A-induced phasic contractions of isolated rat ureters was evaluated by organ bath studies. Results A 7-day consumption of Fiuggi water in rats reduced urine osmolarity by ~40%. Exposure to Hypotonic Solutions released calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter. This response was abated in a calcium-free medium, after capsaicin desensitization, and in the presence of the unselective transient receptor potential channel antagonist, ruthenium red. Exposure of isolated rat ureteral preparations to a Hypotonic Solution inhibited neurokinin A-evoked phasic contraction. This response was attenuated by capsaicin desensitization and in the presence of the calcitonin gene-related peptide receptor antagonist, calcitonin gene-related peptide8-37. Transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4 antagonists did not affect the neurogenic and calcitonin gene-related peptide-dependent relaxation. Conclusion Present data show that Hypotonic Solution evokes calcitonin gene-related peptide release from capsaicin-sensitive intramural sensory nerves, thus inhibiting ureteral contractility, through a transient receptor potential-dependent mechanism. However, this mechanism does not involve transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4. Future studies with appropriate in vivo models should investigate the hypothesis that hypostenuric urine diffusing into the ureteral tissue might favor ureteral relaxation through this novel mechanism.
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Ureteral relaxation through calcitonin gene‐related peptide release from sensory nerve terminals by Hypotonic Solution
International Journal of Urology, 2015Co-Authors: Serena Materazzi, Daiana Minocci, Gaetano De Siena, Silvia Benemei, Romina NassiniAbstract:Objectives To evaluate the influence of Hypotonic Solutions on ureteral relaxation mediated by the release of calcitonin gene-related peptide from intramural sensory nerve endings. Methods Urine osmolarity of Sprague–Dawley rats drinking water low in salt content (Fiuggi water) or a reference water for 7 days was measured. Release of calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter and urinary bladder by Hypotonic Solutions was assessed by an immunometric assay. The mechanism through which Hypotonic Solutions inhibit neurokinin A-induced phasic contractions of isolated rat ureters was evaluated by organ bath studies. Results A 7-day consumption of Fiuggi water in rats reduced urine osmolarity by ~40%. Exposure to Hypotonic Solutions released calcitonin gene-related peptide-like immunoreactivity from slices of rat ureter. This response was abated in a calcium-free medium, after capsaicin desensitization, and in the presence of the unselective transient receptor potential channel antagonist, ruthenium red. Exposure of isolated rat ureteral preparations to a Hypotonic Solution inhibited neurokinin A-evoked phasic contraction. This response was attenuated by capsaicin desensitization and in the presence of the calcitonin gene-related peptide receptor antagonist, calcitonin gene-related peptide8-37. Transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4 antagonists did not affect the neurogenic and calcitonin gene-related peptide-dependent relaxation. Conclusion Present data show that Hypotonic Solution evokes calcitonin gene-related peptide release from capsaicin-sensitive intramural sensory nerves, thus inhibiting ureteral contractility, through a transient receptor potential-dependent mechanism. However, this mechanism does not involve transient receptor potential vanilloid 1 or transient receptor potential vanilloid 4. Future studies with appropriate in vivo models should investigate the hypothesis that hypostenuric urine diffusing into the ureteral tissue might favor ureteral relaxation through this novel mechanism.