The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Robert M Glaeser - One of the best experts on this subject based on the ideXlab platform.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Ultramicroscopy, 2007Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Beam-induced specimen movement may be the major factor that limits the quality of high-resolution Images of organic specimens. One of the possible measures to improve the situation that was proposed by Henderson and Glaeser [Ultramicroscopy 16 (1985) 139-150], which we refer to here as "stroboscopic Image Capture", is to divide the normal exposure into many successive frames, thus reducing the amount of electron exposure--and possibly the amount of beam-induced movement--per frame. The frames would then be aligned and summed. We have performed preliminary experiments on stroboscopic imaging using a 200-kV electron microscope that was equipped with a high dynamic range Charge-coupled device (CCD) camera for Image recording and a liquid N2-cooled cryoholder. Single-layer paraffin crystals on carbon film were used as a test specimen. The ratio F(g)/F(0) of paraffin reflections, calculated from the Images, serves as our criterion for the Image quality. In the series that were evaluated, no significant improvement of the F(Image)(g)/F(Image)(0) ratio was found, even though the electron exposure per frame was reduced by a factor of 30. A frame-to-frame analysis of Image distortions showed that considerable beam-induced movement had still occurred during each frame. In addition, the paraffin crystal lattice was observed to move relative to the supporting carbon film, a fact that cannot be explained as being an electron-optical effect caused by specimen charging. We conclude that a significant further reduction of the dose per frame (than was possible with this CCD detector) will be needed in order to test whether the frame-to-frame changes ultimately become small enough for stroboscopic Image Capture to show its potential.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Lawrence Berkeley National Laboratory, 2006Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Ultramicroscopy: PDF for review Journal Article ID Title Ultramicroscopy ULTRAM_265 STROBOSCOPIC Image Capture: REDUCING THE DOSE PER FRAME BY A FACTOR OF 30 DOES NOT PREVENT BEAM- INDUCED SPECIMEN MOVEMENT IN PARAFFIN Full-length article 21 Dec 05 Version Article type Submitted Files submitted Name Fig1rev.tif Fig2rev.tif Fig3rev.tif Fig4rev.tif Fig5rev.tif Fig6rev.tif Fig7rev.tif FinalTypke.STROBO_I MAGE.doc Fig No Format Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Manuscript (Microsoft Word) Use Maybe Maybe Maybe Maybe Maybe Maybe Maybe Description
Eric R Fossum - One of the best experts on this subject based on the ideXlab platform.
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A 1.5Mpixel RGBZ CMOS Image sensor for simultaneous color and range Image Capture
Digest of Technical Papers - IEEE International Solid-State Circuits Conference, 2012Co-Authors: Wonjoo Kim, Yoondong Park, Seunghoon Lee, Wang Yibing, Ilia Ovsiannikov, Chilhee Chung, Eric R FossumAbstract:A 1.5Mpixel RGBZ Image sensor that simultaneously Captures color (RGB) and time-of-flight (ToF) range (Z) Images is presented. While ToF sensors are well documented, few, if any, monolithic sensors have been reported that Capture both range and color. In one sensor, a combined pixel structure Captures color and range, but presumably in sequential fields. This approach does not allow simultaneous capturing of range with color, and the pixel performance cannot be optimized for each mode. In thie paper, we introduce a sensor that is designed to Capture color and range simultaneously with individually optimized pixels.
Dieter Typke - One of the best experts on this subject based on the ideXlab platform.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Ultramicroscopy, 2007Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Beam-induced specimen movement may be the major factor that limits the quality of high-resolution Images of organic specimens. One of the possible measures to improve the situation that was proposed by Henderson and Glaeser [Ultramicroscopy 16 (1985) 139-150], which we refer to here as "stroboscopic Image Capture", is to divide the normal exposure into many successive frames, thus reducing the amount of electron exposure--and possibly the amount of beam-induced movement--per frame. The frames would then be aligned and summed. We have performed preliminary experiments on stroboscopic imaging using a 200-kV electron microscope that was equipped with a high dynamic range Charge-coupled device (CCD) camera for Image recording and a liquid N2-cooled cryoholder. Single-layer paraffin crystals on carbon film were used as a test specimen. The ratio F(g)/F(0) of paraffin reflections, calculated from the Images, serves as our criterion for the Image quality. In the series that were evaluated, no significant improvement of the F(Image)(g)/F(Image)(0) ratio was found, even though the electron exposure per frame was reduced by a factor of 30. A frame-to-frame analysis of Image distortions showed that considerable beam-induced movement had still occurred during each frame. In addition, the paraffin crystal lattice was observed to move relative to the supporting carbon film, a fact that cannot be explained as being an electron-optical effect caused by specimen charging. We conclude that a significant further reduction of the dose per frame (than was possible with this CCD detector) will be needed in order to test whether the frame-to-frame changes ultimately become small enough for stroboscopic Image Capture to show its potential.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Lawrence Berkeley National Laboratory, 2006Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Ultramicroscopy: PDF for review Journal Article ID Title Ultramicroscopy ULTRAM_265 STROBOSCOPIC Image Capture: REDUCING THE DOSE PER FRAME BY A FACTOR OF 30 DOES NOT PREVENT BEAM- INDUCED SPECIMEN MOVEMENT IN PARAFFIN Full-length article 21 Dec 05 Version Article type Submitted Files submitted Name Fig1rev.tif Fig2rev.tif Fig3rev.tif Fig4rev.tif Fig5rev.tif Fig6rev.tif Fig7rev.tif FinalTypke.STROBO_I MAGE.doc Fig No Format Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Manuscript (Microsoft Word) Use Maybe Maybe Maybe Maybe Maybe Maybe Maybe Description
Kenneth H Downing - One of the best experts on this subject based on the ideXlab platform.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Ultramicroscopy, 2007Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Beam-induced specimen movement may be the major factor that limits the quality of high-resolution Images of organic specimens. One of the possible measures to improve the situation that was proposed by Henderson and Glaeser [Ultramicroscopy 16 (1985) 139-150], which we refer to here as "stroboscopic Image Capture", is to divide the normal exposure into many successive frames, thus reducing the amount of electron exposure--and possibly the amount of beam-induced movement--per frame. The frames would then be aligned and summed. We have performed preliminary experiments on stroboscopic imaging using a 200-kV electron microscope that was equipped with a high dynamic range Charge-coupled device (CCD) camera for Image recording and a liquid N2-cooled cryoholder. Single-layer paraffin crystals on carbon film were used as a test specimen. The ratio F(g)/F(0) of paraffin reflections, calculated from the Images, serves as our criterion for the Image quality. In the series that were evaluated, no significant improvement of the F(Image)(g)/F(Image)(0) ratio was found, even though the electron exposure per frame was reduced by a factor of 30. A frame-to-frame analysis of Image distortions showed that considerable beam-induced movement had still occurred during each frame. In addition, the paraffin crystal lattice was observed to move relative to the supporting carbon film, a fact that cannot be explained as being an electron-optical effect caused by specimen charging. We conclude that a significant further reduction of the dose per frame (than was possible with this CCD detector) will be needed in order to test whether the frame-to-frame changes ultimately become small enough for stroboscopic Image Capture to show its potential.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Lawrence Berkeley National Laboratory, 2006Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Ultramicroscopy: PDF for review Journal Article ID Title Ultramicroscopy ULTRAM_265 STROBOSCOPIC Image Capture: REDUCING THE DOSE PER FRAME BY A FACTOR OF 30 DOES NOT PREVENT BEAM- INDUCED SPECIMEN MOVEMENT IN PARAFFIN Full-length article 21 Dec 05 Version Article type Submitted Files submitted Name Fig1rev.tif Fig2rev.tif Fig3rev.tif Fig4rev.tif Fig5rev.tif Fig6rev.tif Fig7rev.tif FinalTypke.STROBO_I MAGE.doc Fig No Format Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Manuscript (Microsoft Word) Use Maybe Maybe Maybe Maybe Maybe Maybe Maybe Description
Christopher J Gilpin - One of the best experts on this subject based on the ideXlab platform.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Ultramicroscopy, 2007Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Beam-induced specimen movement may be the major factor that limits the quality of high-resolution Images of organic specimens. One of the possible measures to improve the situation that was proposed by Henderson and Glaeser [Ultramicroscopy 16 (1985) 139-150], which we refer to here as "stroboscopic Image Capture", is to divide the normal exposure into many successive frames, thus reducing the amount of electron exposure--and possibly the amount of beam-induced movement--per frame. The frames would then be aligned and summed. We have performed preliminary experiments on stroboscopic imaging using a 200-kV electron microscope that was equipped with a high dynamic range Charge-coupled device (CCD) camera for Image recording and a liquid N2-cooled cryoholder. Single-layer paraffin crystals on carbon film were used as a test specimen. The ratio F(g)/F(0) of paraffin reflections, calculated from the Images, serves as our criterion for the Image quality. In the series that were evaluated, no significant improvement of the F(Image)(g)/F(Image)(0) ratio was found, even though the electron exposure per frame was reduced by a factor of 30. A frame-to-frame analysis of Image distortions showed that considerable beam-induced movement had still occurred during each frame. In addition, the paraffin crystal lattice was observed to move relative to the supporting carbon film, a fact that cannot be explained as being an electron-optical effect caused by specimen charging. We conclude that a significant further reduction of the dose per frame (than was possible with this CCD detector) will be needed in order to test whether the frame-to-frame changes ultimately become small enough for stroboscopic Image Capture to show its potential.
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stroboscopic Image Capture reducing the dose per frame by a factor of 30 does not prevent beam induced specimen movement in paraffin
Lawrence Berkeley National Laboratory, 2006Co-Authors: Dieter Typke, Christopher J Gilpin, Kenneth H Downing, Robert M GlaeserAbstract:Ultramicroscopy: PDF for review Journal Article ID Title Ultramicroscopy ULTRAM_265 STROBOSCOPIC Image Capture: REDUCING THE DOSE PER FRAME BY A FACTOR OF 30 DOES NOT PREVENT BEAM- INDUCED SPECIMEN MOVEMENT IN PARAFFIN Full-length article 21 Dec 05 Version Article type Submitted Files submitted Name Fig1rev.tif Fig2rev.tif Fig3rev.tif Fig4rev.tif Fig5rev.tif Fig6rev.tif Fig7rev.tif FinalTypke.STROBO_I MAGE.doc Fig No Format Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Figures (TIFF) Manuscript (Microsoft Word) Use Maybe Maybe Maybe Maybe Maybe Maybe Maybe Description
