The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform
James Galagan - One of the best experts on this subject based on the ideXlab platform.
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Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Thuy Nguyen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, R. Baer, Sébastien Lecommandoux, Catherine Klapperich, James Galagan, Allison DennisAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.
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surface Immobilized Nucleic Acid transcription factor quantum dots for biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, Sébastien Lecommandoux, Catherine Klapperich, Thuy T Nguyen, R C Baer, James GalaganAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real-world applications. Here the preparation, characterization, and evaluation of a surface-bound transcription factor-Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)-the analyte-the TetR-QDs release from the surface-bound DNA, resulting in loss of the Forster resonance energy transfer signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µm aTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.
Chloé Grazon - One of the best experts on this subject based on the ideXlab platform.
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Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Thuy Nguyen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, R. Baer, Sébastien Lecommandoux, Catherine Klapperich, James Galagan, Allison DennisAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.
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surface Immobilized Nucleic Acid transcription factor quantum dots for biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, Sébastien Lecommandoux, Catherine Klapperich, Thuy T Nguyen, R C Baer, James GalaganAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real-world applications. Here the preparation, characterization, and evaluation of a surface-bound transcription factor-Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)-the analyte-the TetR-QDs release from the surface-bound DNA, resulting in loss of the Forster resonance energy transfer signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µm aTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.
Mingfu Chen - One of the best experts on this subject based on the ideXlab platform.
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Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Thuy Nguyen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, R. Baer, Sébastien Lecommandoux, Catherine Klapperich, James Galagan, Allison DennisAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.
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surface Immobilized Nucleic Acid transcription factor quantum dots for biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, Sébastien Lecommandoux, Catherine Klapperich, Thuy T Nguyen, R C Baer, James GalaganAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real-world applications. Here the preparation, characterization, and evaluation of a surface-bound transcription factor-Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)-the analyte-the TetR-QDs release from the surface-bound DNA, resulting in loss of the Forster resonance energy transfer signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µm aTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.
Allison Dennis - One of the best experts on this subject based on the ideXlab platform.
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Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Thuy Nguyen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, R. Baer, Sébastien Lecommandoux, Catherine Klapperich, James Galagan, Allison DennisAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.
Nitinun Varongchayakul - One of the best experts on this subject based on the ideXlab platform.
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Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Thuy Nguyen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, R. Baer, Sébastien Lecommandoux, Catherine Klapperich, James Galagan, Allison DennisAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.
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surface Immobilized Nucleic Acid transcription factor quantum dots for biosensing
Advanced Healthcare Materials, 2020Co-Authors: Mingfu Chen, Nitinun Varongchayakul, Chloé Grazon, Margaret Chern, Sébastien Lecommandoux, Catherine Klapperich, Thuy T Nguyen, R C Baer, James GalaganAbstract:Immobilization of biosensors on surfaces is a key step toward development of devices for real-world applications. Here the preparation, characterization, and evaluation of a surface-bound transcription factor-Nucleic Acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)-the analyte-the TetR-QDs release from the surface-bound DNA, resulting in loss of the Forster resonance energy transfer signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µm aTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.
