The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Beatriz Varela - One of the best experts on this subject based on the ideXlab platform.
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impact of an andean breakfast on biochemistry and Immunochemistry laboratory tests an evaluation on behalf colabiocli wg pre latam
Biochemia Medica, 2019Co-Authors: Wilson Bajana, Eduardo Aranda, Maria Elena Arredondo, Lorena Michele Brennanbourdon, Marise Danielle Campelo, Edgar Espinoza, Silvia Flores, Patricia Ochoa, Veronica Vega, Beatriz VarelaAbstract:Introduction In Andean countries, specifically in Ecuador, a food transition in the population has been observed because of economic growth. The Working Group for Preanalytical Phase in Latin America (WG-PRE-LATAM) of the Latin America Confederation of Clinical Biochemistry (COLABIOCLI) was established in 2017, and its main purpose is to study preanalytical variability and establish guidelines for preanalytical procedures in order to be implemented by clinical laboratories and healthcare professionals in Latin America. The aim of this study on behalf of COLABIOCLI WG-PRE-LATAM was to evaluate whether an Andean breakfast can interfere with routine biochemistry and Immunochemistry laboratory tests. Materials and methods We studied 20 healthy volunteers who consumed an Andean breakfast containing a standardized amount of carbohydrates, proteins and lipids. We collected blood specimens for laboratory tests before breakfast and 1, 2, and 4 hours thereafter. Significant differences between samples were assessed by the Wilcoxon ranked-pairs test. Results The Andean breakfast statistically (P ≤ 0.05), modified the results of the following tests: triglycerides, insulin, cortisol, thyroid stimulating hormone, free thyroxine, total protein, albumin, urea, creatinine, lactate dehydrogenase, alkaline phosphatase, amylase, lipase, total bilirubin, direct bilirubin, iron, calcium, phosphorus, magnesium, and uric acid. Conclusions Andean breakfast can influence the routine biochemistry and Immunochemistry laboratory tests and might expose patient safety to some risks. Therefore, the COLABIOCLI WG-PRE-LATAM calls attention and highlights that the fasting time needs to be carefully considered when performing blood testing in order to prevent spurious results and thus, reduce laboratory errors.
Jos P M Wielders - One of the best experts on this subject based on the ideXlab platform.
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a novel c2 transferrin variant interfering with the analysis of carbohydrate deficient transferrin
Clinica Chimica Acta, 2011Co-Authors: Holger K De Wolf, Karin Huijben, Merel Van Wijnen, Menno De Metz, Jos P M WieldersAbstract:Abstract Background Carbohydrate-deficient transferrin (CDT) is used as a marker for chronic alcohol abuse. The presence of genetic transferrin variants might affect an individual's iron status and can interfere with CDT analysis. We report on the identification of a patient carrying a novel transferrin variant. We describe the performance of the various CDT methods in its detection and the associated iron status. Methods DNA of the coding region of transferrin was sequenced and CDT levels were analysed using four different methods: high pressure liquid chromatography (HPLC), capillary zone electrophoresis (CZE), Immunochemistry and iso-electric focussing (IEF). Results A novel transferrin variant, T139M, was found as a heterozygous genotype in the index patient and all of his four living direct family members (c.416 C > p.Thr139Met). CDT analysis of the variant by HPLC and CZE was compromised as a result of the coelution of the different isoforms. CDT levels could be quantified by Immunochemistry. Similar results were obtained using IEF analysis. The presence of the C2 transferrin variant did not affect iron status in any of the investigated patients. Conclusions Transferrin T139M, present as a heterozygous genotype, interferes with CDT analysis by HPLC and CZE but not by Immunochemistry. Physiologically, it appears to be functionally normal.
Robert V Jones - One of the best experts on this subject based on the ideXlab platform.
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evaluation of enu induced gliomas in rats nomenclature Immunochemistry and malignancy
Toxicologic Pathology, 2000Co-Authors: Bernard C Zook, Samuel J Simmens, Robert V JonesAbstract:Rats developed mixed gliomas, oligodendrogliomas, and a few astrocytomas in response to transplacental ethylnitrosourea. The neoplastic cell composition of mixed gliomas must be defined; this study required a 20-80% admixture of neoplastic astrocytes and oligodendroglia for the diagnosis of mixed glioma. A battery of immunoantibodies, including Leu-7, S-100, and vimentin, were helpful in classifying rat gliomas, and the histologic features of each tumor type are described. Other brain tumor characteristics that may decide the outcome of carcinogenicity studies include incidence, multiplicity, latency, fatality, size, and malignancy. The size of tumors was determined by measuring their 3-dimensional volumes. Brain tumor volume was found to be highly correlated with malignancy and fatality. Systematic evaluation of the malignancy of brain tumors is an important but often overlooked adjunct method of measuring the effectiveness of a carcinogen. A system to estimate malignancy, one that grades 9 tumor charact...
Wilson Bajana - One of the best experts on this subject based on the ideXlab platform.
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impact of an andean breakfast on biochemistry and Immunochemistry laboratory tests an evaluation on behalf colabiocli wg pre latam
Biochemia Medica, 2019Co-Authors: Wilson Bajana, Eduardo Aranda, Maria Elena Arredondo, Lorena Michele Brennanbourdon, Marise Danielle Campelo, Edgar Espinoza, Silvia Flores, Patricia Ochoa, Veronica Vega, Beatriz VarelaAbstract:Introduction In Andean countries, specifically in Ecuador, a food transition in the population has been observed because of economic growth. The Working Group for Preanalytical Phase in Latin America (WG-PRE-LATAM) of the Latin America Confederation of Clinical Biochemistry (COLABIOCLI) was established in 2017, and its main purpose is to study preanalytical variability and establish guidelines for preanalytical procedures in order to be implemented by clinical laboratories and healthcare professionals in Latin America. The aim of this study on behalf of COLABIOCLI WG-PRE-LATAM was to evaluate whether an Andean breakfast can interfere with routine biochemistry and Immunochemistry laboratory tests. Materials and methods We studied 20 healthy volunteers who consumed an Andean breakfast containing a standardized amount of carbohydrates, proteins and lipids. We collected blood specimens for laboratory tests before breakfast and 1, 2, and 4 hours thereafter. Significant differences between samples were assessed by the Wilcoxon ranked-pairs test. Results The Andean breakfast statistically (P ≤ 0.05), modified the results of the following tests: triglycerides, insulin, cortisol, thyroid stimulating hormone, free thyroxine, total protein, albumin, urea, creatinine, lactate dehydrogenase, alkaline phosphatase, amylase, lipase, total bilirubin, direct bilirubin, iron, calcium, phosphorus, magnesium, and uric acid. Conclusions Andean breakfast can influence the routine biochemistry and Immunochemistry laboratory tests and might expose patient safety to some risks. Therefore, the COLABIOCLI WG-PRE-LATAM calls attention and highlights that the fasting time needs to be carefully considered when performing blood testing in order to prevent spurious results and thus, reduce laboratory errors.
Jerome S Perlish - One of the best experts on this subject based on the ideXlab platform.
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Immunochemistry of a keratinocyte fibroblast co culture model for reconstruction of human skin
Journal of Histochemistry and Cytochemistry, 1993Co-Authors: Raul Fleischmajer, E D Macdonald, P Contard, Jerome S PerlishAbstract:Our purpose was to determine differentiation markers of an in vitro co-culture model in which fibroblasts grown in a three-dimensional nylon mesh were recombined with human keratinocytes. The cultu...
