Immunoglobulin Fragment

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Jian Shi - One of the best experts on this subject based on the ideXlab platform.

  • Immunoglobulin Fragment F(ab') 2 against RBD potently neutralizes SARS-CoV-2 in vitro.
    Antiviral research, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19, which is caused by the emerging human coronavirus SARS-CoV-2, has become a global pandemic that poses a serious threat to human health. To date, no vaccines or specific antiviral drugs have been approved for the treatment of this disease in clinic. Herein, therapeutic antibodies for SARS-CoV-2 were obtained from hyperimmune equine plasma. First, a recombinant SARS-CoV-2 spike protein receptor-binding domain (RBD) was obtained in gram-level quantities through high-cell density fermentation of Chinese hamster ovary cells. Then, the binding of the RBD to the SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, was verified by several biochemical methods. The efficacy of the RBD in triggering antibody response in vivo was subsequently tested in both mice and equines, and the results showed that the RBD triggered high-titer neutralizing antibody production in vivo. Immunoglobulin F(ab’)2 Fragments were prepared from equine antisera via removal of the Fc region from the Immunoglobulins. Finally, a neutralization test with live virus demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with an EC50 of 0.07 μg/ml and an EC80 of 0.18 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlight RBD-specific equine Immunoglobulin F(ab’)2 Fragment as a candidate for the treatment of SARS-CoV-2.

  • Immunoglobulin Fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro
    2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

  • Immunoglobulin Fragment f ab 2 against rbd potently neutralizes sars cov 2 in vitro
    bioRxiv, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi, Yuan Sun
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

Xiaoyan Pan - One of the best experts on this subject based on the ideXlab platform.

  • Immunoglobulin Fragment F(ab') 2 against RBD potently neutralizes SARS-CoV-2 in vitro.
    Antiviral research, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19, which is caused by the emerging human coronavirus SARS-CoV-2, has become a global pandemic that poses a serious threat to human health. To date, no vaccines or specific antiviral drugs have been approved for the treatment of this disease in clinic. Herein, therapeutic antibodies for SARS-CoV-2 were obtained from hyperimmune equine plasma. First, a recombinant SARS-CoV-2 spike protein receptor-binding domain (RBD) was obtained in gram-level quantities through high-cell density fermentation of Chinese hamster ovary cells. Then, the binding of the RBD to the SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, was verified by several biochemical methods. The efficacy of the RBD in triggering antibody response in vivo was subsequently tested in both mice and equines, and the results showed that the RBD triggered high-titer neutralizing antibody production in vivo. Immunoglobulin F(ab’)2 Fragments were prepared from equine antisera via removal of the Fc region from the Immunoglobulins. Finally, a neutralization test with live virus demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with an EC50 of 0.07 μg/ml and an EC80 of 0.18 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlight RBD-specific equine Immunoglobulin F(ab’)2 Fragment as a candidate for the treatment of SARS-CoV-2.

  • Immunoglobulin Fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro
    2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

  • Immunoglobulin Fragment f ab 2 against rbd potently neutralizes sars cov 2 in vitro
    bioRxiv, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi, Yuan Sun
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

Xiaming Jiang - One of the best experts on this subject based on the ideXlab platform.

  • Immunoglobulin Fragment F(ab') 2 against RBD potently neutralizes SARS-CoV-2 in vitro.
    Antiviral research, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19, which is caused by the emerging human coronavirus SARS-CoV-2, has become a global pandemic that poses a serious threat to human health. To date, no vaccines or specific antiviral drugs have been approved for the treatment of this disease in clinic. Herein, therapeutic antibodies for SARS-CoV-2 were obtained from hyperimmune equine plasma. First, a recombinant SARS-CoV-2 spike protein receptor-binding domain (RBD) was obtained in gram-level quantities through high-cell density fermentation of Chinese hamster ovary cells. Then, the binding of the RBD to the SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, was verified by several biochemical methods. The efficacy of the RBD in triggering antibody response in vivo was subsequently tested in both mice and equines, and the results showed that the RBD triggered high-titer neutralizing antibody production in vivo. Immunoglobulin F(ab’)2 Fragments were prepared from equine antisera via removal of the Fc region from the Immunoglobulins. Finally, a neutralization test with live virus demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with an EC50 of 0.07 μg/ml and an EC80 of 0.18 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlight RBD-specific equine Immunoglobulin F(ab’)2 Fragment as a candidate for the treatment of SARS-CoV-2.

  • Immunoglobulin Fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro
    2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

  • Immunoglobulin Fragment f ab 2 against rbd potently neutralizes sars cov 2 in vitro
    bioRxiv, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi, Yuan Sun
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

Lijuan Fang - One of the best experts on this subject based on the ideXlab platform.

  • Immunoglobulin Fragment F(ab') 2 against RBD potently neutralizes SARS-CoV-2 in vitro.
    Antiviral research, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19, which is caused by the emerging human coronavirus SARS-CoV-2, has become a global pandemic that poses a serious threat to human health. To date, no vaccines or specific antiviral drugs have been approved for the treatment of this disease in clinic. Herein, therapeutic antibodies for SARS-CoV-2 were obtained from hyperimmune equine plasma. First, a recombinant SARS-CoV-2 spike protein receptor-binding domain (RBD) was obtained in gram-level quantities through high-cell density fermentation of Chinese hamster ovary cells. Then, the binding of the RBD to the SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, was verified by several biochemical methods. The efficacy of the RBD in triggering antibody response in vivo was subsequently tested in both mice and equines, and the results showed that the RBD triggered high-titer neutralizing antibody production in vivo. Immunoglobulin F(ab’)2 Fragments were prepared from equine antisera via removal of the Fc region from the Immunoglobulins. Finally, a neutralization test with live virus demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with an EC50 of 0.07 μg/ml and an EC80 of 0.18 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlight RBD-specific equine Immunoglobulin F(ab’)2 Fragment as a candidate for the treatment of SARS-CoV-2.

  • Immunoglobulin Fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro
    2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

  • Immunoglobulin Fragment f ab 2 against rbd potently neutralizes sars cov 2 in vitro
    bioRxiv, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi, Yuan Sun
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

Weijuan Shang - One of the best experts on this subject based on the ideXlab platform.

  • Immunoglobulin Fragment F(ab') 2 against RBD potently neutralizes SARS-CoV-2 in vitro.
    Antiviral research, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19, which is caused by the emerging human coronavirus SARS-CoV-2, has become a global pandemic that poses a serious threat to human health. To date, no vaccines or specific antiviral drugs have been approved for the treatment of this disease in clinic. Herein, therapeutic antibodies for SARS-CoV-2 were obtained from hyperimmune equine plasma. First, a recombinant SARS-CoV-2 spike protein receptor-binding domain (RBD) was obtained in gram-level quantities through high-cell density fermentation of Chinese hamster ovary cells. Then, the binding of the RBD to the SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, was verified by several biochemical methods. The efficacy of the RBD in triggering antibody response in vivo was subsequently tested in both mice and equines, and the results showed that the RBD triggered high-titer neutralizing antibody production in vivo. Immunoglobulin F(ab’)2 Fragments were prepared from equine antisera via removal of the Fc region from the Immunoglobulins. Finally, a neutralization test with live virus demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with an EC50 of 0.07 μg/ml and an EC80 of 0.18 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlight RBD-specific equine Immunoglobulin F(ab’)2 Fragment as a candidate for the treatment of SARS-CoV-2.

  • Immunoglobulin Fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro
    2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.

  • Immunoglobulin Fragment f ab 2 against rbd potently neutralizes sars cov 2 in vitro
    bioRxiv, 2020
    Co-Authors: Xiaoyan Pan, Pengfei Zhou, Tiejiong Fan, Jing Zhang, Xiaoyue Shi, Weijuan Shang, Lijuan Fang, Xiaming Jiang, Jian Shi, Yuan Sun
    Abstract:

    Abstract COVID-19 caused by the emerging human coronavirus, SARS-CoV-2, has become a global pandemic, leading a serious threat to human health. So far, there is none vaccines or specific antiviral drugs approved for that. Therapeutic antibodies for SARS-CoV-2, was obtained from hyper immune equine plasma in this study. Herein, SARS-CoV-2 RBD with gram level were obtained through Chinese hamster ovary cells high-density fermentation. The binding of RBD to SARS-CoV-2 receptor, human ACE2, was verified and the efficacy of RBD in vivo was tested on mice and then on horses. As a result, RBD triggered high-titer neutralizing antibodies in vivo, and Immunoglobulin Fragment F(ab’)2 was prepared from horse antisera through removing Fc. Neutralization test demonstrated that RBD-specific F(ab’)2 inhibited SARS-CoV-2 with EC50 at 0.07 μg/ml, showing a potent inhibitory effect on SARS-CoV-2. These results highlights as RBD-specific F(ab’)2 as therapeutic candidate for SARS-CoV-2.