The Experts below are selected from a list of 15837 Experts worldwide ranked by ideXlab platform
Rakesh Anand - One of the best experts on this subject based on the ideXlab platform.
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structure and physical map of 64 variable segments in the 3 0 8 megabase region of the human Immunoglobulin Heavy Chain locus
Nature Genetics, 1993Co-Authors: Fumihiko Matsuda, Euy Kyun Shin, Hitoshi Nagaoka, Ryusuke Matsumura, Makoto Haino, Yosho Fukita, Shigeo Takaishi, Takashi Imai, John H Riley, Rakesh AnandAbstract:Structure and physical map of 64 variable segments in the 3′ 0.8–megabase region of the human Immunoglobulin Heavy–Chain locus
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structure and physical map of 64 variable segments in the 3 0 8 megabase region of the human Immunoglobulin Heavy Chain locus
Nature Genetics, 1993Co-Authors: Fumihiko Matsuda, Euy Kyun Shin, Hitoshi Nagaoka, Ryusuke Matsumura, Makoto Haino, Yosho Fukita, Shigeo Takaishi, Takashi Imai, John H Riley, Rakesh AnandAbstract:We have constructed the physical map of the 0.8 megabase DNA fragment which contains the 3' 64 variable region (V) gene segments of the human Immunoglobulin Heavy Chain (H) locus. The organization of the VH locus showed several features that indicate dynamic reshuffling of this locus. The sequenced 64 VH segments include 31 pseudogenes, of which 24 are highly conserved except for a few point mutations. Comparison of the 64 germline VH sequences shows that each VH family has conserved sequences, suggesting that there might be some genetic or selection mechanisms involved in maintenance of each family. The total number of the human VH segments was estimated to be about 120, including at least 7 orphons.
Fumihiko Matsuda - One of the best experts on this subject based on the ideXlab platform.
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the complete nucleotide sequence of the human Immunoglobulin Heavy Chain variable region locus
Journal of Experimental Medicine, 1998Co-Authors: Fumihiko Matsuda, Kazuo Ishii, Patrice Bourvagnet, K Kuma, Hidenori Hayashida, Takashi Miyata, Tasuku HonjoAbstract:The complete nucleotide sequence of the 957-kb DNA of the human Immunoglobulin Heavy Chain variable (VH) region locus was determined and 43 novel VH segments were identified. The region contains 123 VH segments classifiable into seven different families, of which 79 are pseudogenes. Of the 44 VH segments with an open reading frame, 39 are expressed as Heavy Chain proteins and 1 as mRNA, while the remaining 4 are not found in Immunoglobulin cDNAs. Combinatorial diversity of VH region was calculated to be ∼6,000. Conservation of the promoter and recombination signal sequences was observed to be higher in functional VH segments than in pseudogenes. Phylogenetic analysis of 114 VH segments clearly showed clustering of the VH segments of each family. However, an independent branch in the tree contained a single VH, V4-44.1P, sharing similar levels of homology to human VH families and to those of other vertebrates. Comparison between different copies of homologous units that appear repeatedly across the locus clearly demonstrates that dynamic DNA reorganization of the locus took place at least eight times between 133 and 10 million years ago. One nonImmunoglobulin gene of unknown function was identified in the intergenic region.
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structure and physical map of 64 variable segments in the 3 0 8 megabase region of the human Immunoglobulin Heavy Chain locus
Nature Genetics, 1993Co-Authors: Fumihiko Matsuda, Euy Kyun Shin, Hitoshi Nagaoka, Ryusuke Matsumura, Makoto Haino, Yosho Fukita, Shigeo Takaishi, Takashi Imai, John H Riley, Rakesh AnandAbstract:Structure and physical map of 64 variable segments in the 3′ 0.8–megabase region of the human Immunoglobulin Heavy–Chain locus
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structure and physical map of 64 variable segments in the 3 0 8 megabase region of the human Immunoglobulin Heavy Chain locus
Nature Genetics, 1993Co-Authors: Fumihiko Matsuda, Euy Kyun Shin, Hitoshi Nagaoka, Ryusuke Matsumura, Makoto Haino, Yosho Fukita, Shigeo Takaishi, Takashi Imai, John H Riley, Rakesh AnandAbstract:We have constructed the physical map of the 0.8 megabase DNA fragment which contains the 3' 64 variable region (V) gene segments of the human Immunoglobulin Heavy Chain (H) locus. The organization of the VH locus showed several features that indicate dynamic reshuffling of this locus. The sequenced 64 VH segments include 31 pseudogenes, of which 24 are highly conserved except for a few point mutations. Comparison of the 64 germline VH sequences shows that each VH family has conserved sequences, suggesting that there might be some genetic or selection mechanisms involved in maintenance of each family. The total number of the human VH segments was estimated to be about 120, including at least 7 orphons.
Lisa A Steiner - One of the best experts on this subject based on the ideXlab platform.
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the Immunoglobulin Heavy Chain locus in zebrafish identification and expression of a previously unknown isotype Immunoglobulin z
Nature Immunology, 2005Co-Authors: Nadia Danilova, Jeroen Bussmann, Kerstin Jekosch, Lisa A SteinerAbstract:The Immunoglobulin Heavy-Chain locus in zebrafish: identification and expression of a previously unknown isotype, Immunoglobulin Z
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The Immunoglobulin Heavy-Chain locus in zebrafish: identification and expression of a previously unknown isotype, Immunoglobulin Z
Nature Immunology, 2005Co-Authors: Nadia Danilova, Jeroen Bussmann, Kerstin Jekosch, Lisa A SteinerAbstract:The only Immunoglobulin Heavy-Chain classes known so far in teleosts have been μ and δ. We identify here a previously unknown class, Immunoglobulin ζ, expressed in zebrafish and other teleosts. In the zebrafish Heavy-Chain locus, variable (V) gene segments lie upstream of two tandem diversity, joining and constant (DJC) clusters, resembling the mouse T cell receptor α ( Tcra ) and δ ( Tcrd ) locus. V genes rearrange to (DJC)_ζ or to (DJC)_μ without evidence of switch rearrangement. The zebrafish Immunoglobulin ζ gene ( ighz ) and mouse Tcrd , which are proximal to the V gene array, are expressed earlier in development. In adults, ighz was expressed only in kidney and thymus, which are primary lymphoid organs in teleosts. This additional class adds complexity to the Immunoglobulin repertoire and raises questions concerning the evolution of Immunoglobulins and the regulation of the differential expression of ighz and ighm .
Nadia Danilova - One of the best experts on this subject based on the ideXlab platform.
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the Immunoglobulin Heavy Chain locus in zebrafish identification and expression of a previously unknown isotype Immunoglobulin z
Nature Immunology, 2005Co-Authors: Nadia Danilova, Jeroen Bussmann, Kerstin Jekosch, Lisa A SteinerAbstract:The Immunoglobulin Heavy-Chain locus in zebrafish: identification and expression of a previously unknown isotype, Immunoglobulin Z
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The Immunoglobulin Heavy-Chain locus in zebrafish: identification and expression of a previously unknown isotype, Immunoglobulin Z
Nature Immunology, 2005Co-Authors: Nadia Danilova, Jeroen Bussmann, Kerstin Jekosch, Lisa A SteinerAbstract:The only Immunoglobulin Heavy-Chain classes known so far in teleosts have been μ and δ. We identify here a previously unknown class, Immunoglobulin ζ, expressed in zebrafish and other teleosts. In the zebrafish Heavy-Chain locus, variable (V) gene segments lie upstream of two tandem diversity, joining and constant (DJC) clusters, resembling the mouse T cell receptor α ( Tcra ) and δ ( Tcrd ) locus. V genes rearrange to (DJC)_ζ or to (DJC)_μ without evidence of switch rearrangement. The zebrafish Immunoglobulin ζ gene ( ighz ) and mouse Tcrd , which are proximal to the V gene array, are expressed earlier in development. In adults, ighz was expressed only in kidney and thymus, which are primary lymphoid organs in teleosts. This additional class adds complexity to the Immunoglobulin repertoire and raises questions concerning the evolution of Immunoglobulins and the regulation of the differential expression of ighz and ighm .
Euy Kyun Shin - One of the best experts on this subject based on the ideXlab platform.
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structure and physical map of 64 variable segments in the 3 0 8 megabase region of the human Immunoglobulin Heavy Chain locus
Nature Genetics, 1993Co-Authors: Fumihiko Matsuda, Euy Kyun Shin, Hitoshi Nagaoka, Ryusuke Matsumura, Makoto Haino, Yosho Fukita, Shigeo Takaishi, Takashi Imai, John H Riley, Rakesh AnandAbstract:Structure and physical map of 64 variable segments in the 3′ 0.8–megabase region of the human Immunoglobulin Heavy–Chain locus
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structure and physical map of 64 variable segments in the 3 0 8 megabase region of the human Immunoglobulin Heavy Chain locus
Nature Genetics, 1993Co-Authors: Fumihiko Matsuda, Euy Kyun Shin, Hitoshi Nagaoka, Ryusuke Matsumura, Makoto Haino, Yosho Fukita, Shigeo Takaishi, Takashi Imai, John H Riley, Rakesh AnandAbstract:We have constructed the physical map of the 0.8 megabase DNA fragment which contains the 3' 64 variable region (V) gene segments of the human Immunoglobulin Heavy Chain (H) locus. The organization of the VH locus showed several features that indicate dynamic reshuffling of this locus. The sequenced 64 VH segments include 31 pseudogenes, of which 24 are highly conserved except for a few point mutations. Comparison of the 64 germline VH sequences shows that each VH family has conserved sequences, suggesting that there might be some genetic or selection mechanisms involved in maintenance of each family. The total number of the human VH segments was estimated to be about 120, including at least 7 orphons.
