The Experts below are selected from a list of 537 Experts worldwide ranked by ideXlab platform
Mark E. Meyerhoff - One of the best experts on this subject based on the ideXlab platform.
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Improved Hemocompatibility of silicone rubber extracorporeal tubing via solvent swelling impregnation of s nitroso n acetylpenicillamine snap and evaluation in rabbit thrombogenicity model
Acta Biomaterialia, 2016Co-Authors: Elizabeth J Brisbois, Marcus J Goudie, Terry C Major, Robert H Bartlett, Mark E. Meyerhoff, Hitesh HandaAbstract:Abstract Blood-contacting devices, including extracorporeal circulation (ECC) circuits, can suffer from complications due to platelet activation and thrombus formation. Development of nitric oxide (NO) releasing polymers is one method to Improve Hemocompatibility, taking advantage of the ability of low levels of NO to prevent platelet activation/adhesion. In this study a novel solvent swelling method is used to load the walls of silicone rubber tubing with the NO donor S-nitroso-N-acetylpenicillamine (SNAP). This SNAP-silicone rubber tubing exhibits an NO flux of ca. 1 × 10−10 mol cm−2 min−1, which mimics the range of NO release from the normal endothelium, which is stable for at least 4 h. Images of the tubing before and after swelling, obtained via scanning electron microscopy, demonstrate that this swelling method has little effect on the surface properties of the tubing. The SNAP-loaded silicone rubber and silicone rubber control tubing are used to fabricate ECC circuits that are evaluated in a rabbit model of thrombogenicity. After 4 h of blood flow, the SNAP-loaded silicone rubber circuits were able to preserve the blood platelet count at 64% of baseline (vs. 12% for silicone rubber control). A 67% reduction in the degree of thrombus formation within the thrombogenicity chamber was also observed. This study demonstrates the ability to Improve the Hemocompatibility of existing/commercial silicone rubber tubing via a simple solvent swelling-impregnation technique, which may also be applicable to other silicone-based blood-contacting devices. Statement of Significance Localized nitric oxide (NO) release can be achieved from biomedical grade polymers doped with S-nitroso-N-acetylpenicillamine (SNAP). Despite the promising in vitro and in vivo biocompatibility results reported for these NO releasing polymers, many of these materials may face challenges in being translated to clinical applications, especially in the areas of polymer processing and manufacturing. In this study, we report a solvent swelling-impregnation technique to incorporate SNAP into extracorporeal circuit (ECC) tubing. These NO-releasing ECCs were able to attenuate the activation of platelets and maintain their functionality, while significantly reducing the extent of thrombus formation during 4 h blood flow in the rabbit model of thrombogenicity.
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Electrochemically Modulated Nitric Oxide (NO) Releasing Biomedical Devices via Copper(II)-Tri(2-pyridylmethyl)amine Mediated Reduction of Nitrite
2015Co-Authors: Hang Ren, Nicolai Lehnert, Terry Major, Robert H. Bartlett, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each day, exhibit more than a 100-fold decrease in the amount of surface attached live bacteria (n = 5). These results suggest that this electrochemical NO generation system could provide a robust and highly effective new approach to improving the thromboresistance and antimicrobial properties of intravascular catheters and potentially other biomedical devices
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electrochemically modulated nitric oxide no releasing biomedical devices via copper ii tri 2 pyridylmethyl amine mediated reduction of nitrite
ACS Applied Materials & Interfaces, 2014Co-Authors: Hang Ren, Terry C Major, Robert H Bartlett, Nicolai Lehnert, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each ...
Hang Ren - One of the best experts on this subject based on the ideXlab platform.
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Electrochemically Modulated Nitric Oxide (NO) Releasing Biomedical Devices via Copper(II)-Tri(2-pyridylmethyl)amine Mediated Reduction of Nitrite
2015Co-Authors: Hang Ren, Nicolai Lehnert, Terry Major, Robert H. Bartlett, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each day, exhibit more than a 100-fold decrease in the amount of surface attached live bacteria (n = 5). These results suggest that this electrochemical NO generation system could provide a robust and highly effective new approach to improving the thromboresistance and antimicrobial properties of intravascular catheters and potentially other biomedical devices
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electrochemically modulated nitric oxide no releasing biomedical devices via copper ii tri 2 pyridylmethyl amine mediated reduction of nitrite
ACS Applied Materials & Interfaces, 2014Co-Authors: Hang Ren, Terry C Major, Robert H Bartlett, Nicolai Lehnert, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each ...
Terry C Major - One of the best experts on this subject based on the ideXlab platform.
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Improved Hemocompatibility of silicone rubber extracorporeal tubing via solvent swelling impregnation of s nitroso n acetylpenicillamine snap and evaluation in rabbit thrombogenicity model
Acta Biomaterialia, 2016Co-Authors: Elizabeth J Brisbois, Marcus J Goudie, Terry C Major, Robert H Bartlett, Mark E. Meyerhoff, Hitesh HandaAbstract:Abstract Blood-contacting devices, including extracorporeal circulation (ECC) circuits, can suffer from complications due to platelet activation and thrombus formation. Development of nitric oxide (NO) releasing polymers is one method to Improve Hemocompatibility, taking advantage of the ability of low levels of NO to prevent platelet activation/adhesion. In this study a novel solvent swelling method is used to load the walls of silicone rubber tubing with the NO donor S-nitroso-N-acetylpenicillamine (SNAP). This SNAP-silicone rubber tubing exhibits an NO flux of ca. 1 × 10−10 mol cm−2 min−1, which mimics the range of NO release from the normal endothelium, which is stable for at least 4 h. Images of the tubing before and after swelling, obtained via scanning electron microscopy, demonstrate that this swelling method has little effect on the surface properties of the tubing. The SNAP-loaded silicone rubber and silicone rubber control tubing are used to fabricate ECC circuits that are evaluated in a rabbit model of thrombogenicity. After 4 h of blood flow, the SNAP-loaded silicone rubber circuits were able to preserve the blood platelet count at 64% of baseline (vs. 12% for silicone rubber control). A 67% reduction in the degree of thrombus formation within the thrombogenicity chamber was also observed. This study demonstrates the ability to Improve the Hemocompatibility of existing/commercial silicone rubber tubing via a simple solvent swelling-impregnation technique, which may also be applicable to other silicone-based blood-contacting devices. Statement of Significance Localized nitric oxide (NO) release can be achieved from biomedical grade polymers doped with S-nitroso-N-acetylpenicillamine (SNAP). Despite the promising in vitro and in vivo biocompatibility results reported for these NO releasing polymers, many of these materials may face challenges in being translated to clinical applications, especially in the areas of polymer processing and manufacturing. In this study, we report a solvent swelling-impregnation technique to incorporate SNAP into extracorporeal circuit (ECC) tubing. These NO-releasing ECCs were able to attenuate the activation of platelets and maintain their functionality, while significantly reducing the extent of thrombus formation during 4 h blood flow in the rabbit model of thrombogenicity.
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electrochemically modulated nitric oxide no releasing biomedical devices via copper ii tri 2 pyridylmethyl amine mediated reduction of nitrite
ACS Applied Materials & Interfaces, 2014Co-Authors: Hang Ren, Terry C Major, Robert H Bartlett, Nicolai Lehnert, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each ...
Robert H Bartlett - One of the best experts on this subject based on the ideXlab platform.
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Improved Hemocompatibility of silicone rubber extracorporeal tubing via solvent swelling impregnation of s nitroso n acetylpenicillamine snap and evaluation in rabbit thrombogenicity model
Acta Biomaterialia, 2016Co-Authors: Elizabeth J Brisbois, Marcus J Goudie, Terry C Major, Robert H Bartlett, Mark E. Meyerhoff, Hitesh HandaAbstract:Abstract Blood-contacting devices, including extracorporeal circulation (ECC) circuits, can suffer from complications due to platelet activation and thrombus formation. Development of nitric oxide (NO) releasing polymers is one method to Improve Hemocompatibility, taking advantage of the ability of low levels of NO to prevent platelet activation/adhesion. In this study a novel solvent swelling method is used to load the walls of silicone rubber tubing with the NO donor S-nitroso-N-acetylpenicillamine (SNAP). This SNAP-silicone rubber tubing exhibits an NO flux of ca. 1 × 10−10 mol cm−2 min−1, which mimics the range of NO release from the normal endothelium, which is stable for at least 4 h. Images of the tubing before and after swelling, obtained via scanning electron microscopy, demonstrate that this swelling method has little effect on the surface properties of the tubing. The SNAP-loaded silicone rubber and silicone rubber control tubing are used to fabricate ECC circuits that are evaluated in a rabbit model of thrombogenicity. After 4 h of blood flow, the SNAP-loaded silicone rubber circuits were able to preserve the blood platelet count at 64% of baseline (vs. 12% for silicone rubber control). A 67% reduction in the degree of thrombus formation within the thrombogenicity chamber was also observed. This study demonstrates the ability to Improve the Hemocompatibility of existing/commercial silicone rubber tubing via a simple solvent swelling-impregnation technique, which may also be applicable to other silicone-based blood-contacting devices. Statement of Significance Localized nitric oxide (NO) release can be achieved from biomedical grade polymers doped with S-nitroso-N-acetylpenicillamine (SNAP). Despite the promising in vitro and in vivo biocompatibility results reported for these NO releasing polymers, many of these materials may face challenges in being translated to clinical applications, especially in the areas of polymer processing and manufacturing. In this study, we report a solvent swelling-impregnation technique to incorporate SNAP into extracorporeal circuit (ECC) tubing. These NO-releasing ECCs were able to attenuate the activation of platelets and maintain their functionality, while significantly reducing the extent of thrombus formation during 4 h blood flow in the rabbit model of thrombogenicity.
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electrochemically modulated nitric oxide no releasing biomedical devices via copper ii tri 2 pyridylmethyl amine mediated reduction of nitrite
ACS Applied Materials & Interfaces, 2014Co-Authors: Hang Ren, Terry C Major, Robert H Bartlett, Nicolai Lehnert, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each ...
Nicolai Lehnert - One of the best experts on this subject based on the ideXlab platform.
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Electrochemically Modulated Nitric Oxide (NO) Releasing Biomedical Devices via Copper(II)-Tri(2-pyridylmethyl)amine Mediated Reduction of Nitrite
2015Co-Authors: Hang Ren, Nicolai Lehnert, Terry Major, Robert H. Bartlett, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each day, exhibit more than a 100-fold decrease in the amount of surface attached live bacteria (n = 5). These results suggest that this electrochemical NO generation system could provide a robust and highly effective new approach to improving the thromboresistance and antimicrobial properties of intravascular catheters and potentially other biomedical devices
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electrochemically modulated nitric oxide no releasing biomedical devices via copper ii tri 2 pyridylmethyl amine mediated reduction of nitrite
ACS Applied Materials & Interfaces, 2014Co-Authors: Hang Ren, Terry C Major, Robert H Bartlett, Nicolai Lehnert, Mark E. MeyerhoffAbstract:A controllable and inexpensive electrochemical nitric oxide (NO) release system is demonstrated to Improve Hemocompatibility and reduce bacterial biofilm formation on biomedical devices. Nitric oxide is produced from the electrochemical reduction of nitrite using a copper(II)-tri(2-pyridylmethyl)amine (Cu(II)TPMA) complex as a mediator, and the temporal profile of NO release can be modulated readily by applying different cathodic potentials. Single lumen and dual lumen silicone rubber catheters are employed as initial model biomedical devices incorporating this novel NO release approach. The modified catheters can release a steady, physiologically-relevant flux of NO for more than 7 days. Both single and dual lumen catheters with continuous NO release exhibit greatly reduced thrombus formation on their surfaces after short-term 7-h intravascular placement in rabbit veins (p < 0.02, n = 6). Three day in vitro antimicrobial experiments, in which the catheters are “turned on” for only 3 h of NO release each ...
