Indigenous Microorganism

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Liu N - One of the best experts on this subject based on the ideXlab platform.

  • Treatment of the nitrobenzene and aniline-contaminated groundwater by activated Indigenous Microorganism
    Environmental Pollution & Control, 2012
    Co-Authors: Liu N
    Abstract:

    The nitrobenzene and aniline-contaminated groundwater was selected as the object to study the performance of activated Indigenous Microorganism for nitrobenzene and aniline degradation.In this paper,different activators(lactose;Na2HPO4;Na2HPO4+lactose;ethanol;beef extract;peptone) were added to contaminated groundwater to active the Indigenous Microorganisms,the dehydrogenase activity of water sample was measured at the 3th d and water samples was analyzed by full scan GC/MS at the 9th d.The results indicated that the relative growth rate of Microorganisms was up to 157.2% after adding lactose in water sample and the degradation rate of nitrobenzene and aniline increased by 14.90% and 0.79% respectively;Na2HPO4 and ethanol had little activation effect on Indigenous Microorganism,the growth rate of Microorganisms and the pollutant degradation rate presented no obvious changes.Adding Na2HPO4+lactose in water sample,relative growth rate of Microorganisms was 180.3% and the degradation rate nitrobenzene and aniline elevated 24.20% and 1.21% respectively;adding beef extract in water sample,the relative growth rate of Microorganisms was 830.7% and the degradation rate of nitrobenzene and aniline elevated 99.99% and 99.67% respectively;adding peptone in water sample,the relative growth rate of Microorganisms was 686.0% and the degradation rate of nitrobenzene and aniline elevated 99.33% and 58.94% respectively.The full scan GC/MS spectra showed that the degradation of chloroaniline,1-methyl-4-nitrobenzene and other organic matter was enhanced after adding different activators,so it was feasible to remediate the organic contamination groundwater by activating the Indigenous microbial.

She Yuehui - One of the best experts on this subject based on the ideXlab platform.

  • the study on Indigenous Microorganism paraffin inhibition and removal technology of xinjiang high temperature petroleum reservoir
    Chemistry & Bioengineering, 2007
    Co-Authors: She Yuehui
    Abstract:

    Two kinds of Microorganism are got from the second factory eight area P2W1 of Kelamayi Oilfield,which are living with wax as carbon source.These bacteria can resist high temperature,produce biosurfactant and organic acid,and can degrade crude oil and paraffin.The mixed bacterium DPM4-2,after cultivated at 60℃ over 7 days,can lead to degrading of the paraffin deposition and paraffin by 43.1% and 54.9%,respectively.The oil viscosity decreases by 27.3% ~ 43.6%.It's concluded that this mixed bacterium is suitable for the paraffin control of the second factory eight area P2W1 of Kelamayi Oilfield.

  • pilot test of Indigenous Microorganism flooding in kongdian oilfield
    Petroleum Exploration and Development, 2005
    Co-Authors: Feng Qingxian, Yang Huaijun, Wang Jianqiang, She Yuehui, N I Fangtian
    Abstract:

    Indigenous microbial flooding technology utilizes the bacteria existing in the reservoir(Indigenous Microorganism).With residual oil near injection wells as carbon source,together with additional basic nutrients mixed with air,the useful Indigenous microbes are activated to multiply and metabolize rapidly.The Microorganisms decompose crude oil,produce flooding agents and improve reservoir ecosystem and properties of formation water and oil,thus the residual oil flow property is promoted and oil recovery enhanced.The technology is different from the MEOR which is widely carried out in the present.Based on the research of reservoir geology,the analyses of development situation and bio-chemical parameters,and the feasi bility study on Indigenous microbial flooding,the pilot test program was determined for the high porosity-permeability bottom water heavy oil reservoir in the No.2 Block of Kongdian area,Dagang Oilfield.The test with the injection of nutrient-air mixture was carried out in March,2001,and lasted for 3 years.Field performance monitoring and production tracking results show: ① the Indigenous bacteria were activated with the number increased 3-7 orders compared with pre-test,the methane metabolization rate increased 4-78 times,concentration of metabolites increased 16-208 times and sulfate reduction rate increased with no produce of H_2S;②properties of oil and formation water were improved; ③oil and gas production increased in wells,and total oil increase was 17,866t up to Feb.,2005.The Indigenous Microbial Flooding Technology is successfully used in Kongdian Oilfield,which would serve as an example to EOR development of old oilfields in high water cut stage.

  • enhanced viscous oil recovery by activating Indigenous Microorganism
    Journal of Oil and Gas Technology, 2005
    Co-Authors: She Yuehui, Yuan Zhihua
    Abstract:

    The microbiologic technique for enhanced oil recovery, which is based on the geochemistry activity of Indigenous Microorganisms, pilot testes hed been made during the period from 2001 to 2004 in Kongdian area of Dagang Oilfield. Its activation is obtained by injecting aerated water and compensating mineral salt containing nitrate and phosphate through injection wells. The technique induces the Microorganisms activating, which is taken place in nearby injectors first, and then performed on the area along the direction of hydrodynamics. At the first stage, the activation of oxygen-hydrocarbon degrading microbes are reduced, which results in the increase of hydrocarbonate and acetic acid; at the second stage, the methanogenic bacteria are activated in the anaerobic regions. The Indigenous MEOR pilot test indicates that the microbial indexes in formation water are related with the production in producing wells. In the study more than 16000 ton of crude oil are produced.

Max M Haggblom - One of the best experts on this subject based on the ideXlab platform.

  • reductive dehalogenation activity of Indigenous Microorganism in sediments of the hackensack river new jersey
    Environmental Pollution, 2016
    Co-Authors: Seo Yean Sohn, Max M Haggblom
    Abstract:

    Organohalogen pollutants are of concern in many river and estuarine environments, such as the New York-New Jersey Harbor estuary and its tributaries. The Hackensack River is contaminated with various metals, hydrocarbons and halogenated organics, including polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins. In order to examine the potential for microbial reductive dechlorination by Indigenous Microorganisms, sediment samples were collected from five different estuarine locations along the Hackensack River. Hexachlorobenzene (HCB), hexabromobenzene (HBB), and pentachloroaniline (PCA) were selected as model organohalogen pollutants to assess anaerobic dehalogenating potential. Dechlorinating activity of HCB and PCA was observed in sediment microcosms for all sampling sites. HCB was dechlorinated via pentachlorobenzene (PeCB) and trichlorobenzene (TriCB) to dichlorobenzene (DCB). PCA was dechlorinated via tetrachloroaniline (TeCA), trichloroanilines (TriCA), and dichloroanilines (DCA) to monochloroaniline (MCA). No HBB debromination was observed over 12 months of incubation. However, with HCB as a co-substrate slow HBB debromination was observed with production of tetrabromobenzene (TeBB) and tribromobenzene (TriBB). Chloroflexi specific 16S rRNA gene PCR-DGGE followed by sequence analysis detected Dehalococcoides species in sediments of the freshwater location, but not in the estuarine site. Analysis targeting 12 putative reductive dehalogenase (rdh) genes showed that these were enriched concomitant with HCB or PCA dechlorination in freshwater sediment microcosms.

Chen Meime - One of the best experts on this subject based on the ideXlab platform.

  • optimization of ecological conditions for heavy oil contaminated soil remediation by an Indigenous Microorganism
    Journal of Anhui Agricultural Sciences, 2013
    Co-Authors: Chen Meime
    Abstract:

    [Objective] The study aimed to discuss the optimal ecological conditions for heavy oil-contaminated soil remediation by an Indigenous Microorganism.[Method]Indigenous bacteria segregated from heavy oil-contaminated soil in Liaohe River basin were used to restore the heavy oil-contaminated soil.Firstly,the effects of pH,oil concentration,nutrient elements and inoculum volume on degradation efficiency of petroleum hydrocarbons in the soil were analyzed through a single factor test,and the optimal ecological conditions for heavy oil-contaminated soil remediation were determined through an orthogonal test.[Result]Oil concentration was the most important factor influencing the degradation efficiency of petroleum hydrocarbons,followed by inoculum volume and pH.The optimal ecological conditions for heavy oil-contaminated soil remediation were as follows: dosages of KNO3 and KH2PO4 were 0.45 and 0.10 g respectively;pH was 8;inoculum volume was 2 ml;oil concentration was 0.2%.Under the optimal ecological conditions,the degradation efficiency reached 61.87%.[Conclusion] The research could provide scientific references for microbial remediation of heavy oil-contaminated soil.

Yuan Zhihua - One of the best experts on this subject based on the ideXlab platform.

  • enhanced viscous oil recovery by activating Indigenous Microorganism
    Journal of Oil and Gas Technology, 2005
    Co-Authors: She Yuehui, Yuan Zhihua
    Abstract:

    The microbiologic technique for enhanced oil recovery, which is based on the geochemistry activity of Indigenous Microorganisms, pilot testes hed been made during the period from 2001 to 2004 in Kongdian area of Dagang Oilfield. Its activation is obtained by injecting aerated water and compensating mineral salt containing nitrate and phosphate through injection wells. The technique induces the Microorganisms activating, which is taken place in nearby injectors first, and then performed on the area along the direction of hydrodynamics. At the first stage, the activation of oxygen-hydrocarbon degrading microbes are reduced, which results in the increase of hydrocarbonate and acetic acid; at the second stage, the methanogenic bacteria are activated in the anaerobic regions. The Indigenous MEOR pilot test indicates that the microbial indexes in formation water are related with the production in producing wells. In the study more than 16000 ton of crude oil are produced.