The Experts below are selected from a list of 150 Experts worldwide ranked by ideXlab platform
Akio Yamazaki - One of the best experts on this subject based on the ideXlab platform.
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A novel role of RGS9: Inhibition of retinal guanylyl cyclase
Molecular and Cellular Biochemistry, 2002Co-Authors: Vladimir A. Bondarenko, Russell K. Yamazaki, Akio YamazakiAbstract:Cyclic GMP plays a key role in retinal phototransduction and its photoreceptor concentration is precisely controlled by the cooperative action of cGMP phosphodiesterase (PDE) and retinal guanylyl cyclase (retGC). However, studies of the relationship between these two systems have focused only on a Ca^2+-mediated, Indirect Connection. This article summarizes our studies strongly suggesting that RGS9-1 is directly involved in the cooperative action of PDE and retGC, and that this ingenious mechanism plays an important role in tuning of cGMP concentration in photoreceptors.
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Inhibition of retinal guanylyl cyclase by the RGS9-1 N-terminus.
Biochemical and biophysical research communications, 2001Co-Authors: Vladimir A. Bondarenko, Akio YamazakiAbstract:Abstract Cyclic GMP plays a key role in retinal phototransduction and its photoreceptor concentration is precisely controlled by the cooperative action of cGMP phosphodiesterase (PDE) and retinal guanylyl cyclase (retGC). However, studies of the relationship between these two systems have focused only on a Ca 2+ -mediated, Indirect Connection. Using a retinal “regulator of G-protein signaling” (RGS9-1) and its fragments, we show that the N-terminus of RGS9-1 inhibits retGC activity. We also indicate that the GGL domain and/or the RGS domain function as an internal suppressor against the N-terminus, suggesting that proteins bound to these domains regulate the inhibitory activity of the N-terminus. Direct interaction of retGC with RGS9-1 and its N-terminus is also proved by immunoprecipitation and an overlay technique. Since RGS9-1 also controls the lifetime of transducin-activated PDE through regulating GTPase activity of transducin, this study strongly suggests that RGS9-1 mediates the direct interaction between PDE and retGC systems, and that this ingenious mechanism plays an important role in tuning of cGMP concentration in photoreceptors.
Vladimir A. Bondarenko - One of the best experts on this subject based on the ideXlab platform.
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A novel role of RGS9: Inhibition of retinal guanylyl cyclase
Molecular and Cellular Biochemistry, 2002Co-Authors: Vladimir A. Bondarenko, Russell K. Yamazaki, Akio YamazakiAbstract:Cyclic GMP plays a key role in retinal phototransduction and its photoreceptor concentration is precisely controlled by the cooperative action of cGMP phosphodiesterase (PDE) and retinal guanylyl cyclase (retGC). However, studies of the relationship between these two systems have focused only on a Ca^2+-mediated, Indirect Connection. This article summarizes our studies strongly suggesting that RGS9-1 is directly involved in the cooperative action of PDE and retGC, and that this ingenious mechanism plays an important role in tuning of cGMP concentration in photoreceptors.
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Inhibition of retinal guanylyl cyclase by the RGS9-1 N-terminus.
Biochemical and biophysical research communications, 2001Co-Authors: Vladimir A. Bondarenko, Akio YamazakiAbstract:Abstract Cyclic GMP plays a key role in retinal phototransduction and its photoreceptor concentration is precisely controlled by the cooperative action of cGMP phosphodiesterase (PDE) and retinal guanylyl cyclase (retGC). However, studies of the relationship between these two systems have focused only on a Ca 2+ -mediated, Indirect Connection. Using a retinal “regulator of G-protein signaling” (RGS9-1) and its fragments, we show that the N-terminus of RGS9-1 inhibits retGC activity. We also indicate that the GGL domain and/or the RGS domain function as an internal suppressor against the N-terminus, suggesting that proteins bound to these domains regulate the inhibitory activity of the N-terminus. Direct interaction of retGC with RGS9-1 and its N-terminus is also proved by immunoprecipitation and an overlay technique. Since RGS9-1 also controls the lifetime of transducin-activated PDE through regulating GTPase activity of transducin, this study strongly suggests that RGS9-1 mediates the direct interaction between PDE and retGC systems, and that this ingenious mechanism plays an important role in tuning of cGMP concentration in photoreceptors.
Bin Lai - One of the best experts on this subject based on the ideXlab platform.
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a conditioning strengthened circuit from ca1 of dorsal hippocampus to basolateral amygdala participates in morphine withdrawal memory retrieval
Frontiers in Neuroscience, 2020Co-Authors: Zixuan Cao, Da Shao, Jiaojiao Song, Huan Sheng, Li Yang, Dongyang Cui, Ming Chen, Fei Zhao, Minhua Luo, Bin LaiAbstract:Conditioned context-induced retrieval of drug withdrawal memory contributes to drug relapse. The basolateral amygdala (BLA) is an important brain region that is involved in conditioned context-induced retrieval of morphine withdrawal memory. However, the upstream pathways of the activation of the BLA by conditioned context remains to be studied. The present results show that the CA1 of dorsal hippocampus is an upstream brain region of the activation of the BLA during conditioned context-induced morphine withdrawal memory retrieval; the Indirect Connection from the CA1 of dorsal hippocampus to the BLA is enhanced in mice with conditioned place aversion (CPA); the postrhinal cortex (POR) is a brain region that connects the CA1 of dorsal hippocampus and the activation of the BLA during conditioned context-induced retrieval of morphine-withdrawal memory. These results suggest that a conditioning-strengthened Indirect circuit from the CA1 of dorsal hippocampus to the BLA through the POR participates in morphine withdrawal memory retrieval.
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a series circuit from ca1 of dorsal hippocampus to basolateral amygdala via postrhinal cortex participates in conditioned context induced retrieval of morphine withdrawal memory
Social Science Research Network, 2019Co-Authors: Zixuan Cao, Da Shao, Jiaojiao Song, Huan Sheng, Li Yang, Dongyang Cui, Ming Chen, Bin Lai, Ping ZhengAbstract:Background: Conditioned context-induced retrieval of drug withdrawal memory contributes to drug relapse. The basolateral amygdala (BLA) plays an important role in conditioned context-induced retrieval of morphine withdrawal memory. However, the upstream pathways of the activation of the BLA by conditioned context remains to be studied. Methods: Using the conditioned place aversion model, chemical-genetic method, trans-neuronal virus tracing technique and immunohistochemical method, we studied the upstream neural circuits of the activation of the BLA during conditioned context-induced retrieval of morphine withdrawal memory. Findings: The present results show that the dorsal CA1 of the hippocampus is an upstream brain region of the activation of the BLA during conditioned context-induced withdrawal memory retrieval; the Indirect Connection from dorsal CA1 of the hippocampus to the BLA is enhanced in mice with conditioned place aversion; the postrhinal cortex (POR) is a brain region that connects the dorsal CA1 of the hippocampus and the activation of the BLA in conditioned context-induced retrieval of morphine withdrawal memory. Interpretation: These results suggest that a series circuit from CA1 of dorsal hippocampus to basolateral amygdala via postrhinal cortex participates in conditioned context-induced retrieval of morphine withdrawal memory. Funding Statement: This study was supported by the National Program of Basic Research sponsored by the Ministry of Science and Technology of China (2009CB52201 and 2013CB835100) and Project of Foundation of National Natural Science of China (31121061, 91332204, 81371466 and 31070932). Declaration of Interests: All authors declare no conflicts of interest. Ethics Approval Statement: All experimental procedures conformed to Fudan University as well as international guidelines on the ethical use of animals. All efforts were made to minimize animal suffering and reduce the number of animals used.
Predrag Zekonja - One of the best experts on this subject based on the ideXlab platform.
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supply water temperature regulation problems in district heating network with both direct and Indirect Connection
Energy and Buildings, 1998Co-Authors: Branislav Jacimovic, Branislav Zivkovic, Srbislav Genic, Predrag ZekonjaAbstract:General form of equations for hot water temperature in house installation and district heating system according to the outside temperature are well known. The influence of heat exchanger type and its fouling on heat output in DH system is experimentally determined. When both direct and Indirect Connections are applied to the same district heating network, certain exploitation problems have been noticed due to real behavior of heat exchangers. Two technical possibilities are discussed in order to overcome problems.
Zixuan Cao - One of the best experts on this subject based on the ideXlab platform.
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a conditioning strengthened circuit from ca1 of dorsal hippocampus to basolateral amygdala participates in morphine withdrawal memory retrieval
Frontiers in Neuroscience, 2020Co-Authors: Zixuan Cao, Da Shao, Jiaojiao Song, Huan Sheng, Li Yang, Dongyang Cui, Ming Chen, Fei Zhao, Minhua Luo, Bin LaiAbstract:Conditioned context-induced retrieval of drug withdrawal memory contributes to drug relapse. The basolateral amygdala (BLA) is an important brain region that is involved in conditioned context-induced retrieval of morphine withdrawal memory. However, the upstream pathways of the activation of the BLA by conditioned context remains to be studied. The present results show that the CA1 of dorsal hippocampus is an upstream brain region of the activation of the BLA during conditioned context-induced morphine withdrawal memory retrieval; the Indirect Connection from the CA1 of dorsal hippocampus to the BLA is enhanced in mice with conditioned place aversion (CPA); the postrhinal cortex (POR) is a brain region that connects the CA1 of dorsal hippocampus and the activation of the BLA during conditioned context-induced retrieval of morphine-withdrawal memory. These results suggest that a conditioning-strengthened Indirect circuit from the CA1 of dorsal hippocampus to the BLA through the POR participates in morphine withdrawal memory retrieval.
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a series circuit from ca1 of dorsal hippocampus to basolateral amygdala via postrhinal cortex participates in conditioned context induced retrieval of morphine withdrawal memory
Social Science Research Network, 2019Co-Authors: Zixuan Cao, Da Shao, Jiaojiao Song, Huan Sheng, Li Yang, Dongyang Cui, Ming Chen, Bin Lai, Ping ZhengAbstract:Background: Conditioned context-induced retrieval of drug withdrawal memory contributes to drug relapse. The basolateral amygdala (BLA) plays an important role in conditioned context-induced retrieval of morphine withdrawal memory. However, the upstream pathways of the activation of the BLA by conditioned context remains to be studied. Methods: Using the conditioned place aversion model, chemical-genetic method, trans-neuronal virus tracing technique and immunohistochemical method, we studied the upstream neural circuits of the activation of the BLA during conditioned context-induced retrieval of morphine withdrawal memory. Findings: The present results show that the dorsal CA1 of the hippocampus is an upstream brain region of the activation of the BLA during conditioned context-induced withdrawal memory retrieval; the Indirect Connection from dorsal CA1 of the hippocampus to the BLA is enhanced in mice with conditioned place aversion; the postrhinal cortex (POR) is a brain region that connects the dorsal CA1 of the hippocampus and the activation of the BLA in conditioned context-induced retrieval of morphine withdrawal memory. Interpretation: These results suggest that a series circuit from CA1 of dorsal hippocampus to basolateral amygdala via postrhinal cortex participates in conditioned context-induced retrieval of morphine withdrawal memory. Funding Statement: This study was supported by the National Program of Basic Research sponsored by the Ministry of Science and Technology of China (2009CB52201 and 2013CB835100) and Project of Foundation of National Natural Science of China (31121061, 91332204, 81371466 and 31070932). Declaration of Interests: All authors declare no conflicts of interest. Ethics Approval Statement: All experimental procedures conformed to Fudan University as well as international guidelines on the ethical use of animals. All efforts were made to minimize animal suffering and reduce the number of animals used.