The Experts below are selected from a list of 33 Experts worldwide ranked by ideXlab platform
Martin Grosell - One of the best experts on this subject based on the ideXlab platform.
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fractionation of the gulf toadfish intestinal precipitate organic matrix reveals potential functions of Individual proteins
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2017Co-Authors: Kevin L Schauer, Martin GrosellAbstract:Abstract The regulatory mechanisms behind the production of CaCO 3 in the marine teleost intestine are poorly studied despite being essential for osmoregulation and responsible for a conservatively estimated 3–15% of annual oceanic CaCO 3 production. It has recently been reported that the intestinally derived precipitates produced by fish as a byproduct of their osmoregulatory strategy form in conjunction with a proteinaceous matrix containing nearly 150 unique proteins. The Individual functions of these proteins have not been the subject of investigation until now. Here, organic matrix was extracted from precipitates produced by Gulf toadfish ( Opsanus beta ) and the matrix proteins were fractionated by their charge using strong anion exchange chromatography. The precipitation regulatory abilities of the Individual fractions were then analyzed using a recently developed in vitro Calcification assay, and the protein constituents of each fraction were determined by mass spectrometry. The different fractions were found to have differing effects on both the rate of carbonate mineral production, as well as the morphology of the crystals that form. Using data collected from the Calcification assay as well as the mass spectrometry experiments, Individual Calcification promotional indices were calculated for each protein, giving the first insight into the functions each of these matrix proteins may play in regulating precipitation.
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Fractionation of the Gulf toadfish intestinal precipitate organic matrix reveals potential functions of Individual proteins Part A Molecular & integrative physiology
Comparative Biochemistry and Physiology, 2017Co-Authors: Kevin L Schauer, Martin GrosellAbstract:The regulatory mechanisms behind the production of CaCO3 in the marine teleost intestine are poorly studied despite being essential for osmoregulation and responsible for a conservatively estimated 3–15% of annual oceanic CaCO3 production. It has recently been reported that the intestinally derived precipitates produced by fish as a byproduct of their osmoregulatory strategy form in conjunction with a proteinaceous matrix containing nearly 150 unique proteins. The Individual functions of these proteins have not been the subject of investigation until now. Here, organic matrix was extracted from precipitates produced by Gulf toadfish (Opsanus beta) and the matrix proteins were fractionated by their charge using strong anion exchange chromatography. The precipitation regulatory abilities of the Individual fractions were then analyzed using a recently developed in vitro Calcification assay, and the protein constituents of each fraction were determined by mass spectrometry. The different fractions were found to have differing effects on both the rate of carbonate mineral production, as well as the morphology of the crystals that form. Using data collected from the Calcification assay as well as the mass spectrometry experiments, Individual Calcification promotional indices were calculated for each protein, giving the first insight into the functions each of these matrix proteins may play in regulating precipitation.
Kevin L Schauer - One of the best experts on this subject based on the ideXlab platform.
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fractionation of the gulf toadfish intestinal precipitate organic matrix reveals potential functions of Individual proteins
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2017Co-Authors: Kevin L Schauer, Martin GrosellAbstract:Abstract The regulatory mechanisms behind the production of CaCO 3 in the marine teleost intestine are poorly studied despite being essential for osmoregulation and responsible for a conservatively estimated 3–15% of annual oceanic CaCO 3 production. It has recently been reported that the intestinally derived precipitates produced by fish as a byproduct of their osmoregulatory strategy form in conjunction with a proteinaceous matrix containing nearly 150 unique proteins. The Individual functions of these proteins have not been the subject of investigation until now. Here, organic matrix was extracted from precipitates produced by Gulf toadfish ( Opsanus beta ) and the matrix proteins were fractionated by their charge using strong anion exchange chromatography. The precipitation regulatory abilities of the Individual fractions were then analyzed using a recently developed in vitro Calcification assay, and the protein constituents of each fraction were determined by mass spectrometry. The different fractions were found to have differing effects on both the rate of carbonate mineral production, as well as the morphology of the crystals that form. Using data collected from the Calcification assay as well as the mass spectrometry experiments, Individual Calcification promotional indices were calculated for each protein, giving the first insight into the functions each of these matrix proteins may play in regulating precipitation.
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Fractionation of the Gulf toadfish intestinal precipitate organic matrix reveals potential functions of Individual proteins Part A Molecular & integrative physiology
Comparative Biochemistry and Physiology, 2017Co-Authors: Kevin L Schauer, Martin GrosellAbstract:The regulatory mechanisms behind the production of CaCO3 in the marine teleost intestine are poorly studied despite being essential for osmoregulation and responsible for a conservatively estimated 3–15% of annual oceanic CaCO3 production. It has recently been reported that the intestinally derived precipitates produced by fish as a byproduct of their osmoregulatory strategy form in conjunction with a proteinaceous matrix containing nearly 150 unique proteins. The Individual functions of these proteins have not been the subject of investigation until now. Here, organic matrix was extracted from precipitates produced by Gulf toadfish (Opsanus beta) and the matrix proteins were fractionated by their charge using strong anion exchange chromatography. The precipitation regulatory abilities of the Individual fractions were then analyzed using a recently developed in vitro Calcification assay, and the protein constituents of each fraction were determined by mass spectrometry. The different fractions were found to have differing effects on both the rate of carbonate mineral production, as well as the morphology of the crystals that form. Using data collected from the Calcification assay as well as the mass spectrometry experiments, Individual Calcification promotional indices were calculated for each protein, giving the first insight into the functions each of these matrix proteins may play in regulating precipitation.
Shuang-shuang Zhao - One of the best experts on this subject based on the ideXlab platform.
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Calcification of thyroid nodules increases shear-wave speed (SWS) measurement: using multiple Calcification-specific SWS cutoff values outperforms a single uniform cutoff value in diagnosing malignant thyroid nodules.
Oncotarget, 2016Co-Authors: Bao-ding Chen, Yi-feng Zhang, Bo-ji Liu, Le-hang Guo, Chongke Zhao, Dan Wang, Shuang-shuang ZhaoAbstract:. // Bao-Ding Chen 1,2,3,4,5 , Hui-Xiong Xu 1,2,3,4 , Yi-Feng Zhang 1,2,3,4 , Bo-Ji Liu 1,2,3,4 , Le-Hang Guo 1,2,3,4 , Dan-Dan Li 1,2,3,4 , Chong-Ke Zhao 1,2,3,4 , Xiao-Long Li 1,2,3,4 , Dan Wang 1,2,3,4 and Shuang-Shuang Zhao 5 1 Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Ultrasound Research and Educational Institute, Tongji University School of Medicine, Shanghai, China 2 Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Clinical College of Nanjing Medical University, Shanghai, China 3 Thyroid Institute, Tongji University School of Medicine, Shanghai, China 4 Shanghai Center of Thyroid Diseases, Shanghai, China 5 Department of Medical Ultrasound, Affiliated Hospital of Jiangsu University, Zhenjiang, China Correspondence to: Hui-Xiong Xu, email: // Keywords : thyroid nodule, Calcification, ultrasound, shear-wave elastography, point shear-wave measurement Received : June 30, 2016 Accepted : August 26, 2016 Published : August 31, 2016 Abstract Conventional ultrasound cannot satisfactorily distinguish malignant and benign thyroid nodules. Shear-wave elastography (SWE) can evaluate tissue stiffness and complement conventional ultrasound in diagnosing malignant nodules. However, Calcification of nodules may affect the results of SWE. The purposes of this study are to compare the differences of shear-wave speed (SWS) measurement among different Calcification groups and compare the diagnostic performance between using a single uniform SWS cutoff value and multiple Individual Calcification-specific cutoff values using technique of point SWS measurement. We retrospectively identified 517 thyroid nodules (346 benign and 171 malignant nodules) examined by conventional ultrasound and point SWS measurement. There were 177 non-calcified, 159 micro-calcified and 181 macro-calcified nodules. The diagnostic performance was evaluated by receiver operating characteristic (ROC) curve and area under the curve (AUC) was computed. The mean SWS in malignant nodules more than doubled that of benign nodules (4.81±2.03 m/s vs. 2.29±0.99 m/s, p 2.42 m/s), micro- (SWS >2.88 m/s) and macro-Calcification (SWS >3.59 m/s) nodules in the whole group, the AUC was 0.859 (95% confidence interval [CI], 0.826-0.888), which was significantly better than the AUC of 0.816 (95% CI, 0.780-0.848) if a single uniform cutoff value (SWS >2.72 m/s) was applied to all the nodules regardless of Calcification status (p=0.011). The cutoff values of SWS for different calcified nodules warrant future prospective validation.
Bao-ding Chen - One of the best experts on this subject based on the ideXlab platform.
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Calcification of thyroid nodules increases shear-wave speed (SWS) measurement: using multiple Calcification-specific SWS cutoff values outperforms a single uniform cutoff value in diagnosing malignant thyroid nodules.
Oncotarget, 2016Co-Authors: Bao-ding Chen, Yi-feng Zhang, Bo-ji Liu, Le-hang Guo, Chongke Zhao, Dan Wang, Shuang-shuang ZhaoAbstract:. // Bao-Ding Chen 1,2,3,4,5 , Hui-Xiong Xu 1,2,3,4 , Yi-Feng Zhang 1,2,3,4 , Bo-Ji Liu 1,2,3,4 , Le-Hang Guo 1,2,3,4 , Dan-Dan Li 1,2,3,4 , Chong-Ke Zhao 1,2,3,4 , Xiao-Long Li 1,2,3,4 , Dan Wang 1,2,3,4 and Shuang-Shuang Zhao 5 1 Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Ultrasound Research and Educational Institute, Tongji University School of Medicine, Shanghai, China 2 Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Clinical College of Nanjing Medical University, Shanghai, China 3 Thyroid Institute, Tongji University School of Medicine, Shanghai, China 4 Shanghai Center of Thyroid Diseases, Shanghai, China 5 Department of Medical Ultrasound, Affiliated Hospital of Jiangsu University, Zhenjiang, China Correspondence to: Hui-Xiong Xu, email: // Keywords : thyroid nodule, Calcification, ultrasound, shear-wave elastography, point shear-wave measurement Received : June 30, 2016 Accepted : August 26, 2016 Published : August 31, 2016 Abstract Conventional ultrasound cannot satisfactorily distinguish malignant and benign thyroid nodules. Shear-wave elastography (SWE) can evaluate tissue stiffness and complement conventional ultrasound in diagnosing malignant nodules. However, Calcification of nodules may affect the results of SWE. The purposes of this study are to compare the differences of shear-wave speed (SWS) measurement among different Calcification groups and compare the diagnostic performance between using a single uniform SWS cutoff value and multiple Individual Calcification-specific cutoff values using technique of point SWS measurement. We retrospectively identified 517 thyroid nodules (346 benign and 171 malignant nodules) examined by conventional ultrasound and point SWS measurement. There were 177 non-calcified, 159 micro-calcified and 181 macro-calcified nodules. The diagnostic performance was evaluated by receiver operating characteristic (ROC) curve and area under the curve (AUC) was computed. The mean SWS in malignant nodules more than doubled that of benign nodules (4.81±2.03 m/s vs. 2.29±0.99 m/s, p 2.42 m/s), micro- (SWS >2.88 m/s) and macro-Calcification (SWS >3.59 m/s) nodules in the whole group, the AUC was 0.859 (95% confidence interval [CI], 0.826-0.888), which was significantly better than the AUC of 0.816 (95% CI, 0.780-0.848) if a single uniform cutoff value (SWS >2.72 m/s) was applied to all the nodules regardless of Calcification status (p=0.011). The cutoff values of SWS for different calcified nodules warrant future prospective validation.
Yi-feng Zhang - One of the best experts on this subject based on the ideXlab platform.
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Calcification of thyroid nodules increases shear-wave speed (SWS) measurement: using multiple Calcification-specific SWS cutoff values outperforms a single uniform cutoff value in diagnosing malignant thyroid nodules.
Oncotarget, 2016Co-Authors: Bao-ding Chen, Yi-feng Zhang, Bo-ji Liu, Le-hang Guo, Chongke Zhao, Dan Wang, Shuang-shuang ZhaoAbstract:. // Bao-Ding Chen 1,2,3,4,5 , Hui-Xiong Xu 1,2,3,4 , Yi-Feng Zhang 1,2,3,4 , Bo-Ji Liu 1,2,3,4 , Le-Hang Guo 1,2,3,4 , Dan-Dan Li 1,2,3,4 , Chong-Ke Zhao 1,2,3,4 , Xiao-Long Li 1,2,3,4 , Dan Wang 1,2,3,4 and Shuang-Shuang Zhao 5 1 Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Ultrasound Research and Educational Institute, Tongji University School of Medicine, Shanghai, China 2 Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Clinical College of Nanjing Medical University, Shanghai, China 3 Thyroid Institute, Tongji University School of Medicine, Shanghai, China 4 Shanghai Center of Thyroid Diseases, Shanghai, China 5 Department of Medical Ultrasound, Affiliated Hospital of Jiangsu University, Zhenjiang, China Correspondence to: Hui-Xiong Xu, email: // Keywords : thyroid nodule, Calcification, ultrasound, shear-wave elastography, point shear-wave measurement Received : June 30, 2016 Accepted : August 26, 2016 Published : August 31, 2016 Abstract Conventional ultrasound cannot satisfactorily distinguish malignant and benign thyroid nodules. Shear-wave elastography (SWE) can evaluate tissue stiffness and complement conventional ultrasound in diagnosing malignant nodules. However, Calcification of nodules may affect the results of SWE. The purposes of this study are to compare the differences of shear-wave speed (SWS) measurement among different Calcification groups and compare the diagnostic performance between using a single uniform SWS cutoff value and multiple Individual Calcification-specific cutoff values using technique of point SWS measurement. We retrospectively identified 517 thyroid nodules (346 benign and 171 malignant nodules) examined by conventional ultrasound and point SWS measurement. There were 177 non-calcified, 159 micro-calcified and 181 macro-calcified nodules. The diagnostic performance was evaluated by receiver operating characteristic (ROC) curve and area under the curve (AUC) was computed. The mean SWS in malignant nodules more than doubled that of benign nodules (4.81±2.03 m/s vs. 2.29±0.99 m/s, p 2.42 m/s), micro- (SWS >2.88 m/s) and macro-Calcification (SWS >3.59 m/s) nodules in the whole group, the AUC was 0.859 (95% confidence interval [CI], 0.826-0.888), which was significantly better than the AUC of 0.816 (95% CI, 0.780-0.848) if a single uniform cutoff value (SWS >2.72 m/s) was applied to all the nodules regardless of Calcification status (p=0.011). The cutoff values of SWS for different calcified nodules warrant future prospective validation.
