The Experts below are selected from a list of 4842 Experts worldwide ranked by ideXlab platform

Bastian Blombach - One of the best experts on this subject based on the ideXlab platform.

  • Vibrio natriegens — eine neue Plattform für die Biotechnologie?
    Biospektrum, 2019
    Co-Authors: Felix L. Müller, Bastian Blombach
    Abstract:

    High productivity is a crucial factor for biotechnological processes. Vibrio natriegens is the fastest growing non-pathogenic organism on our planet and therefore has the potential to speed-up Industrial Fermentation processes, cell free protein synthesis and routines in molecular biology.

  • high substrate uptake rates empower vibrio natriegens as production host for Industrial biotechnology
    Applied and Environmental Microbiology, 2017
    Co-Authors: Eugenia Hoffart, Sebastian Grenz, Julian Lange, Robert Nitschel, Felix Muller, Andreas Schwentner, Andre Feith, Mira Lenferslucker, Ralf Takors, Bastian Blombach
    Abstract:

    : The productivity of Industrial Fermentation processes is essentially limited by the biomass-specific substrate consumption rate (qS ) of the applied microbial production system. Since qS depends on the growth rate (μ), we highlight the potential of the fastest-growing nonpathogenic bacterium, Vibrio natriegens, as a novel candidate for future biotechnological processes. V. natriegens grows rapidly in BHIN complex medium with a μ of up to 4.43 h-1 (doubling time of 9.4 min) as well as in minimal medium supplemented with various Industrially relevant substrates. Bioreactor cultivations in minimal medium with glucose showed that V. natriegens possesses an exceptionally high qS under aerobic (3.90 ± 0.08 g g-1 h-1) and anaerobic (7.81 ± 0.71 g g-1 h-1) conditions. Fermentations with resting cells of genetically engineered V. natriegens under anaerobic conditions yielded an overall volumetric productivity of 0.56 ± 0.10 g alanine liter-1 min-1 (i.e., 34 g liter-1 h-1). These inherent properties render V. natriegens a promising new microbial platform for future Industrial Fermentation processes operating with high productivity.IMPORTANCE Low conversion rates are one major challenge to realizing microbial Fermentation processes for the production of commodities operating competitively with existing petrochemical approaches. For this reason, we screened for a novel platform organism possessing characteristics superior to those of traditionally employed microbial systems. We identified the fast-growing V. natriegens, which exhibits a versatile metabolism and shows striking growth and conversion rates, as a solid candidate to reach outstanding productivities. Due to these inherent characteristics, V. natriegens can speed up common laboratory routines, is suitable for already existing production procedures, and forms an excellent foundation for engineering next-generation bioprocesses.

Murray Moo-young - One of the best experts on this subject based on the ideXlab platform.

  • Clean-in-place systems for Industrial bioreactors: Design, validation and operation
    Journal of Industrial Microbiology, 1994
    Co-Authors: Yusuf Chisti, Murray Moo-young
    Abstract:

    Guidelines for design, validation and operation of clean-in-place systems for Industrial Fermentation plant are presented. Design of vessels, surface finishes, materials of construction, types and locations of valves are some of the considerations addressed. Requisite levels of turbulence for cleaning of pipes and vessels are discussed as well as typical cleaning sequences. Recommendations for validation of cleaning are presented and the significance of design of cleaning systems in ensuring satisfactory validation is pointed out. To the extent possible, validation of cleaning should be carried out with real process soil or soil closely simulating actual Fermentation broths.

  • Short Review Clean-in-place systems for Industrial bioreactors: design, validation and operation
    1994
    Co-Authors: Yusuf Chisti, Murray Moo-young
    Abstract:

    SUMMARY Guidelines for design, validation and operation of clean-in-place systems for Industrial Fermentation plant are presented. Design of vessels, surface finishes, materials of construction, types and locations of valves are some of the considerations addressed. Requisite levels of turbulence for cleaning of pipes and vessels are discussed as well as typical cleaning sequences. Recommendations for validation of cleaning are presented and the significance of design of cleaning systems in ensuring satisfactory validation is pointed out. To the extent possible, validation of cleaning shOuld be carried out with real process soil or soil closely simulating actual Fermentation broths.

Patrick N. Royce - One of the best experts on this subject based on the ideXlab platform.

Eugenia Hoffart - One of the best experts on this subject based on the ideXlab platform.

  • high substrate uptake rates empower vibrio natriegens as production host for Industrial biotechnology
    Applied and Environmental Microbiology, 2017
    Co-Authors: Eugenia Hoffart, Sebastian Grenz, Julian Lange, Robert Nitschel, Felix Muller, Andreas Schwentner, Andre Feith, Mira Lenferslucker, Ralf Takors, Bastian Blombach
    Abstract:

    : The productivity of Industrial Fermentation processes is essentially limited by the biomass-specific substrate consumption rate (qS ) of the applied microbial production system. Since qS depends on the growth rate (μ), we highlight the potential of the fastest-growing nonpathogenic bacterium, Vibrio natriegens, as a novel candidate for future biotechnological processes. V. natriegens grows rapidly in BHIN complex medium with a μ of up to 4.43 h-1 (doubling time of 9.4 min) as well as in minimal medium supplemented with various Industrially relevant substrates. Bioreactor cultivations in minimal medium with glucose showed that V. natriegens possesses an exceptionally high qS under aerobic (3.90 ± 0.08 g g-1 h-1) and anaerobic (7.81 ± 0.71 g g-1 h-1) conditions. Fermentations with resting cells of genetically engineered V. natriegens under anaerobic conditions yielded an overall volumetric productivity of 0.56 ± 0.10 g alanine liter-1 min-1 (i.e., 34 g liter-1 h-1). These inherent properties render V. natriegens a promising new microbial platform for future Industrial Fermentation processes operating with high productivity.IMPORTANCE Low conversion rates are one major challenge to realizing microbial Fermentation processes for the production of commodities operating competitively with existing petrochemical approaches. For this reason, we screened for a novel platform organism possessing characteristics superior to those of traditionally employed microbial systems. We identified the fast-growing V. natriegens, which exhibits a versatile metabolism and shows striking growth and conversion rates, as a solid candidate to reach outstanding productivities. Due to these inherent characteristics, V. natriegens can speed up common laboratory routines, is suitable for already existing production procedures, and forms an excellent foundation for engineering next-generation bioprocesses.

Mario L. Lopes - One of the best experts on this subject based on the ideXlab platform.

  • Yeast selection for fuel ethanol production in Brazil
    FEMS Yeast Research, 2008
    Co-Authors: Leonardo Cruz Basso, Henrique V. De Amorim, Antonio J. De Oliveira, Mario L. Lopes
    Abstract:

    Brazil is one of the largest ethanol biofuel producers and exporters in the world and its production has increased steadily during the last three decades. The increasing efficiency of Brazilian ethanol plants has been evident due to the many technological contributions. As far as yeast is concerned, few publications are available regarding the Industrial Fermentation processes in Brazil. The present paper reports on a yeast selection program performed during the last 12 years aimed at selecting Saccharomyces cerevisiae strains suitable for Fermentation of sugar cane substrates (cane juice and molasses) with cell recycle, as it is conducted in Brazilian bioethanol plants. As a result, some evidence is presented showing the positive impact of selected yeast strains in increasing ethanol yield and reducing production costs, due to their higher Fermentation performance (high ethanol yield, reduced glycerol and foam formation, maintenance of high viability during recycling and very high implantation capability into Industrial fermenters). Results also suggest that the great yeast biodiversity found in distillery environments could be an important source of strains. This is because during yeast cell recycling, selective pressure (an adaptive evolution) is imposed on cells, leading to strains with higher tolerance to the stressful conditions of the Industrial Fermentation.