The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform
Songlin Chen - One of the best experts on this subject based on the ideXlab platform.
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a genome scan for quantitative trait loci associated with vibrio anguillarum Infection Resistance in japanese flounder paralichthys olivaceus by bulked segregant analysis
Marine Biotechnology, 2014Co-Authors: Yingping Zhang, Yongsheng Tian, Xiaolin Liao, Han Deng, Wenlong Li, Ying Xu, Lei Wang, Songlin ChenAbstract:A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum Resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and Resistance bulk DNA. Bulked segregant analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and Resistance bulk (P = 0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker analysis. Using two different models, 11–17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease Resistance against V. anguillarum Infection.
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mhc polymorphism and disease Resistance to vibrio anguillarum in 12 selective japanese flounder paralichthys olivaceus families
Fish & Shellfish Immunology, 2008Co-Authors: Songlin Chen, Yongsheng TianAbstract:Abstract Genetic variation in the major histocompatibility complex (MHC) class IIB was tested in Japanese flounder (Paralichthys olivaceus) for survival after challenge with bacterial Infection. The material consisted of 6000 Japanese flounder from 60 families challenged with Vibrio anguillarum, which causes significantly different mortality in flounder families. Five individuals from each of six high-Resistance (HR) and six low-Resistance (LR) families were screened for their MHC class IIB genotypes using sequence analysis. High polymorphism of MHC IIB gene and at least three loci were discovered in Japanese flounder and the rate of dN occurred at a significantly higher frequency than that of dS in PBR. Among 60 individuals, 76 alleles were discovered and 15 alleles were used to study associations between alleles and Resistance to disease. We found highly significant associations between Resistance towards infectious disease caused by V. anguillarum and MHC class IIB polymorphism in Japanese flounder. Some alleles appeared in both HR and LR families, while some alleles were only discovered in HR or LR families. One allele, Paol-DAB*4301, was significantly more prevalent in HR families than in LR families (P = 0.023). Paol-DAB*0601, Paol-DAB*0801, Paol-DAB*2001, Paol-DAB*3803 were discovered in two HR families with high frequency. One allele, Paol-DAB*1601, was discovered in three LR families. The steady heredity of MHC class IIB alleles was observed, and the family having Paol-DAB*4301 alleles was confirmed with higher Resistance to V. anguillarum. This study confirmed the association between alleles of MHC class IIB gene and disease Resistance, and also detected some alleles which might be correlated with high bacterial Infection Resistance. The disease Resistance-related MHC markers could be used for molecular marker-assisted selective breeding in the flounder.
Yongsheng Tian - One of the best experts on this subject based on the ideXlab platform.
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a genome scan for quantitative trait loci associated with vibrio anguillarum Infection Resistance in japanese flounder paralichthys olivaceus by bulked segregant analysis
Marine Biotechnology, 2014Co-Authors: Yingping Zhang, Yongsheng Tian, Xiaolin Liao, Han Deng, Wenlong Li, Ying Xu, Lei Wang, Songlin ChenAbstract:A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum Resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and Resistance bulk DNA. Bulked segregant analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and Resistance bulk (P = 0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker analysis. Using two different models, 11–17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease Resistance against V. anguillarum Infection.
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mhc polymorphism and disease Resistance to vibrio anguillarum in 12 selective japanese flounder paralichthys olivaceus families
Fish & Shellfish Immunology, 2008Co-Authors: Songlin Chen, Yongsheng TianAbstract:Abstract Genetic variation in the major histocompatibility complex (MHC) class IIB was tested in Japanese flounder (Paralichthys olivaceus) for survival after challenge with bacterial Infection. The material consisted of 6000 Japanese flounder from 60 families challenged with Vibrio anguillarum, which causes significantly different mortality in flounder families. Five individuals from each of six high-Resistance (HR) and six low-Resistance (LR) families were screened for their MHC class IIB genotypes using sequence analysis. High polymorphism of MHC IIB gene and at least three loci were discovered in Japanese flounder and the rate of dN occurred at a significantly higher frequency than that of dS in PBR. Among 60 individuals, 76 alleles were discovered and 15 alleles were used to study associations between alleles and Resistance to disease. We found highly significant associations between Resistance towards infectious disease caused by V. anguillarum and MHC class IIB polymorphism in Japanese flounder. Some alleles appeared in both HR and LR families, while some alleles were only discovered in HR or LR families. One allele, Paol-DAB*4301, was significantly more prevalent in HR families than in LR families (P = 0.023). Paol-DAB*0601, Paol-DAB*0801, Paol-DAB*2001, Paol-DAB*3803 were discovered in two HR families with high frequency. One allele, Paol-DAB*1601, was discovered in three LR families. The steady heredity of MHC class IIB alleles was observed, and the family having Paol-DAB*4301 alleles was confirmed with higher Resistance to V. anguillarum. This study confirmed the association between alleles of MHC class IIB gene and disease Resistance, and also detected some alleles which might be correlated with high bacterial Infection Resistance. The disease Resistance-related MHC markers could be used for molecular marker-assisted selective breeding in the flounder.
Lei Wang - One of the best experts on this subject based on the ideXlab platform.
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a genome scan for quantitative trait loci associated with vibrio anguillarum Infection Resistance in japanese flounder paralichthys olivaceus by bulked segregant analysis
Marine Biotechnology, 2014Co-Authors: Yingping Zhang, Yongsheng Tian, Xiaolin Liao, Han Deng, Wenlong Li, Ying Xu, Lei Wang, Songlin ChenAbstract:A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum Resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and Resistance bulk DNA. Bulked segregant analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and Resistance bulk (P = 0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker analysis. Using two different models, 11–17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease Resistance against V. anguillarum Infection.
Beatriz Gutiérrez-gil - One of the best experts on this subject based on the ideXlab platform.
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Exploring the mechanisms of Resistance to Teladorsagia circumcincta Infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes
Veterinary Research, 2018Co-Authors: Praveen K. Chitneedi, Aroa Suárez-vega, María Martínez-valladares, Juan José Arranz, Beatriz Gutiérrez-gilAbstract:AbstractThe present study exploited the RNA-seq technology to analyze the transcriptome of target tissues affected by the Teladorsagia circumcincta Infection in two groups of adult ewes showing different statuses against gastrointestinal nematode (GIN) Infection with the aim of identifying genes linked to GIN Infection Resistance in sheep. For this, based on the accumulated faecal egg count of 18 adult Churra ewes subjected to a first experimental Infection with T. circumcincta, six ewes were classified as resistant and six others as susceptible to the Infection. These 12 animals were dewormed and infected again. After humanitarian sacrifice of these 12 animals at day 7 post-Infection, RNA samples were obtained from abomasal mucosa and lymph node tissues and RNA-Seq datasets were generated using an Illumina HiSeq 2000 sequencer. The distribution of the genes based on their expression level were very similar among the two different tissues and conditions. The differential expression analysis performed with two software (DESeq and EdgeR) only identified common differentially expressed genes (DEGs), a total of 106, in the lymph node samples which were considered as GIN-activated. The enrichment analysis performed for these GIN-activated genes identified some pathways related to cytokine-mediated immune response and the PPARG signaling pathway as well as disease terms related to inflammation and gastro-intestinal diseases as enriched. A systematic comparison with the results of previous studies confirmed the involvement of genes such as ITLN2, CLAC1 and galectins, in the immune mechanism activated against T. circumcincta in resistant sheep.
Hirotaka Kanuka - One of the best experts on this subject based on the ideXlab platform.
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activation of imd pathway in hemocyte confers Infection Resistance through humoral response in drosophila
Biochemical and Biophysical Research Communications, 2013Co-Authors: Bryce Nelson, Tina Freisinger, Kenichi Ishii, Kiyoshi Okado, Naoaki Shinzawa, Shinya Fukumoto, Hirotaka KanukaAbstract:Abstract Upon microbial invasion the innate immune system of Drosophila melanogaster mounts a response that comes in two distinct but complimentary forms, humoral and cellular. A screen to find genes capable of conferring Resistance to the Gram-positive Staphylococcus aureus upon ectopic expression in immune response tissues uncovered imd gene. This Resistance was not dependent on cellular defenses but rather likely a result of upregulation of the humoral response through increased expression of antimicrobial peptides, including a Toll pathway reporter gene drosomycin . Taken together it appears that Imd pathway is capable of playing a role in Resistance to the Gram-positive S. aureus , counter to notions of traditional roles of the Imd pathway thought largely to responsible for Resistance to Gram-negative bacteria.