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Albina Altemani - One of the best experts on this subject based on the ideXlab platform.
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is the immunohistochemical study of the Inflammatory Infiltrate helpful in distinguishing villitis of unknown etiology from non specific infection villitis
Placenta, 2005Co-Authors: H Brito, Priscila Bianchi Juliano, C Altemani, Albina AltemaniAbstract:Villitis is an important placental lesion, which can be caused by specific maternal infections, e.g. rubella, toxoplasmosis, etc, but most of them are of unknown etiology [1]. The clinical significance of villitis depends on the etiology and severity of fetal infection [2] and may lead to intrauterine fetal death, abortion, malformations and fetal growth retardation. The latter has been the major clinical association of villitis of unknown etiology (VUE) [2e4]. Morphologically, infectious villitis may show no specific features and can masquerade as VUE [5]. Both lesions are characterized by chronic Inflammatory cells, mainly macrophages and lymphocytes, in the placental villous stroma [2,5]. There are few immunomorphological studies about the immune cells in infectious villitis as well as in VUE [6e9]. Labarrere et al. [6] described that in placentas with VUE the predominant Inflammatory cells were activated macrophages expressing class II major histocompatibility complex antigens and helper T cells. Few CD8C T cells and no B cells were detected in the same lesions [6]. These findings contrast with those in villitis caused by maternal infection by Trypanosoma cruzi (Chagas’ disease), in which CD8C cells outnumbered CD4C T cells [7]. The aim of this study was to analyze whether immunohistochemical study of the Inflammatory Infiltrate in chronic villitis without specific morphological features could distinguish VUE from infectious villitis. All cases of villitis should be reported, particularly in developing countries, where there is a higher incidence of intrauterine infection [10] and the immunohistochemical study could provide objectivity that conventional histological methods lack.
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immunohistochemical characterization of the Inflammatory Infiltrate in placental chagas disease a qualitative and quantitative analysis
American Journal of Tropical Medicine and Hygiene, 2000Co-Authors: Albina Altemani, Achilea L Bittencourt, Ana Maria A LanaAbstract:Chagas' disease, a systemic illness endemic to some regions of South America, is caused by the protozoan Trypanosoma cruzi. Transplacental infection may occur during any phase and cause fetal death. This study is the first to characterize the Inflammatory cells in chagasic villitis by immunohistochemistry. Paraffin sections of 8 placentas with villitis by T. cruzi (4 live births and 4 stillbirths), as well as 8 control placentas without inflammation, were stained with hematoxylin and eosin, monoclonal antibodies for CD45RO, CD20, CD45RO/OPD4, CD8, HNKI, CD15, MAC387, and CD68 proteins, and a polyclonal antibody for S-100 protein. Quantification of positive cells was performed in 3 different high-power fields. In all cases of chagasic villitis, the Inflammatory Infiltrate was composed mainly of CD68+ macrophages, T lymphocytes, and a few natural killer cells. Among T cells, CD8+ cells outnumbered CD4+ cells in all placentas (CD4+:CD8+ ratios ranged from 0.04 to 0.38). B cells were absent or rare. In stillbirths, villitis was diffuse and severe with numerous T. cruzi, while in live births it was focal with few parasites. Other features that characterized villitis in stillbirths were 1) frequent trophoblastic necrosis, 2) presence of MAC387+ macrophages and CD15+ granulocytes attached to the sites of trophoblastic necrosis, 3) low CD4+: CD8+ ratios in most cases, 4) increased numbers of S-100 positive cells in the villous stroma. In conclusion, CD68+ macrophages and CD8+ T lymphocytes were the major cell population in villitis caused by T. cruzi. However, the pattern of Inflammatory reaction differed between stillbirths and live births and was probably related to the number of parasites in the placental villi.
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Immunohistochemical study of the Inflammatory Infiltrate in villitis of unknown etiology. A qualitative and quantitative analysis.
Pathology research and practice, 1992Co-Authors: Albina AltemaniAbstract:Summary Villitis is characterized by an Inflammatory Infiltrate within the substance of the chorionic villi. Quantitative and qualitative analyses of the mononuclear Infiltrate in areas of villitis were performed in placentas with villitis of unknown etiology (VUE). We used a panel of monoclonal antibodies and immunoperoxidase technique in paraffin sections from 17 placental with VUE and 8 without VUE. Macrophages followed by T lymphocytes were the predominant Inflammatory cells in areas of villitis in virtually all cases. B lymphocytes were not observed and monocytes were present usually in small number in 58 per cent of the cases. Mononuclear cells which expressed HLA-DR antigens were found in 75 per cent of the cases. In areas of villitis with trophoblastic necrosis, we found monocytes and some T lymphocytes adhered to them. These cells apparently had migrated from the maternal circulation. We suggest that in areas of villitis with destruction of the trophoblast and its basal membrane the Inflammatory Infiltrate might have a mixture of fetal and maternal cells. The maternal monocytes and T lymphocytes might be attracted to these sites of trophoblastic necrosis and activated due to exposure to fetal MHC antigens of the villous stroma.
Luciano Artese - One of the best experts on this subject based on the ideXlab platform.
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immunohistochemical analysis of Inflammatory Infiltrate in aggressive and chronic periodontitis a comparative study
Clinical Oral Investigations, 2011Co-Authors: Luciano Artese, Maciej Simon, Adriano Piattelli, Daniel Ferrari, Luciana Ap Gouveia Cardoso, Marcelo Faveri, Tatiana Onuma, Marcello Piccirilli, Vittoria PerrottiAbstract:This immunohistochemical study evaluated the Inflammatory Infiltrate with its cluster differentiation markers (CD 4, CD 8, CD 20, and CD 68) in aggressive and chronic periodontitis gingival tissues in order to identify the specific cell distribution. Twenty-seven human gingival biopsies were obtained and analyzed. Fourteen patients were suffering from chronic periodontitis and six from aggressive periodontitis; seven patients with healthy gingiva were included as the control group. The specimens were immunohistochemically stained for anti-CD 4 (T helper cells), anti-CD 8 (T cytotoxic/suppressor), anti CD-20 (B plasma cells) and anti CD-68 (macrophages). Chronic periodontitis samples were mainly dominated by CD 4 and CD 8+ cells. On the contrary, in aggressive periodontitis patients all four cell types (CD 4, CD 8, CD 20 and CD 68 + cells, respectively) were remarkably increased. CD 20+ cells were significantly (p < 0.05) more prevalent in aggressive versus chronic periodontitis. The control samples expressed lower CD 4, CD 8, CD 20 and CD 68+ cells confirming a none Inflammatory state. The present study demonstrates prevalence for CD 20+ cells in aggressive periodontitis lesions. However, further studies need to be performed to confirm and identify a clear pattern of Inflammatory cells and hereafter the mechanisms sustaining the disease.
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Inflammatory Infiltrate microvessel density vascular endothelial growth factor nitric oxide synthase and proliferative activity in soft tissues below intraorally welded titanium bars
Journal of Periodontology, 2010Co-Authors: Stefano Fanali, Adriano Piattelli, Marcello Piccirilli, Vittoria Perrotti, Luciano Riccardi, Laura Ricci, Luciano ArteseAbstract:Background: The aim of the present study is a comparative evaluation of Inflammatory Infiltrate, microvessel density, vascular endothelial growth factor, nitric oxide synthase, and proliferative activity in soft tissues below intraorally welded titanium bars. Methods: Twenty-two patients participated in this study. All patients carried immediately loaded one-stage titanium implants splinted with intraorally welded titanium bars. Each patient underwent two gingival biopsies, a control biopsy harvested from an area of mucosa 5 mm away from the titanium bar and a test biopsy from the mucosa below the titanium bar, which were histologically and immunohistochemically processed. Results: No fractures or radiographically detectable alterations of the welded frameworks were present. In all the cases examined, the average of the modified plaque index was 1, no suppuration or bleeding on probing was present, and probing depth was £3 mm. However, the immunohistochemical analysis revealed some differences. The Inflammatory Infiltrate was mostly present in test sites and its extension was much larger than in control sites. Statistically significant differences were found in microvessel density and Ki-67 expression among control and test groups (P <0.0001). The high intensity of vascular endothelial growth factor, nitric oxide synthase 1, and nitric oxide synthase 3 expression were mainly detected in the test group, whereas the low intensities were mostly expressed in controls, with statistically significant differences (P <0.0001). Conclusions: In the present study, the immunohistochemical analysis shows that the tissues below the titanium bars underwent a higher rate of Inflammatory and reparative processes. However, further long-term studies, where clinical and immunohistochemical data are collected in parallel, should be conducted for a better understanding of the expression pattern of inflammation markers. J Periodontol 2010;81:748-757.
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Inflammatory Infiltrate microvessel density nitric oxide synthase expression vascular endothelial growth factor expression and proliferative activity in peri implant soft tissues around titanium and zirconium oxide healing caps
Journal of Periodontology, 2006Co-Authors: Marco Degidi, Luciano Artese, Vittoria Perrotti, Antonio Scarano, Peter Gehrke, Adriano PiattelliAbstract:Background: The aim of the present study in humans was to conduct a comparative immunohistochemical evaluation of vascular endothelial growth factor (VEGF) and nitric oxide synthase (NOS) expression, Inflammatory Infiltrate, proliferative activity expression, and microvessel density (MVD) in peri-implant soft tissues of titanium and zirconium oxide healing caps.Methods: Five patients, three men and two women (aged 30 to 66 years; mean: 49 years), participated in this study. All patients received dental implants that were 3.8 mm in diameter and 11 mm in length. All implants were left to heal in a non-submerged (single-stage) mode. Healing caps (3.8 mm in diameter and 3.0 mm in height) were inserted in all implants. Half of the implants were supplied with standard, prefabricated caps of commercially pure titanium, whereas the other half were provided with test zirconium oxide caps. After a 6-month healing period, a gingival biopsy was performed with a circular scalpel (5.5 mm in diameter) around the healing...
Adriano Piattelli - One of the best experts on this subject based on the ideXlab platform.
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immunohistochemical analysis of Inflammatory Infiltrate in aggressive and chronic periodontitis a comparative study
Clinical Oral Investigations, 2011Co-Authors: Luciano Artese, Maciej Simon, Adriano Piattelli, Daniel Ferrari, Luciana Ap Gouveia Cardoso, Marcelo Faveri, Tatiana Onuma, Marcello Piccirilli, Vittoria PerrottiAbstract:This immunohistochemical study evaluated the Inflammatory Infiltrate with its cluster differentiation markers (CD 4, CD 8, CD 20, and CD 68) in aggressive and chronic periodontitis gingival tissues in order to identify the specific cell distribution. Twenty-seven human gingival biopsies were obtained and analyzed. Fourteen patients were suffering from chronic periodontitis and six from aggressive periodontitis; seven patients with healthy gingiva were included as the control group. The specimens were immunohistochemically stained for anti-CD 4 (T helper cells), anti-CD 8 (T cytotoxic/suppressor), anti CD-20 (B plasma cells) and anti CD-68 (macrophages). Chronic periodontitis samples were mainly dominated by CD 4 and CD 8+ cells. On the contrary, in aggressive periodontitis patients all four cell types (CD 4, CD 8, CD 20 and CD 68 + cells, respectively) were remarkably increased. CD 20+ cells were significantly (p < 0.05) more prevalent in aggressive versus chronic periodontitis. The control samples expressed lower CD 4, CD 8, CD 20 and CD 68+ cells confirming a none Inflammatory state. The present study demonstrates prevalence for CD 20+ cells in aggressive periodontitis lesions. However, further studies need to be performed to confirm and identify a clear pattern of Inflammatory cells and hereafter the mechanisms sustaining the disease.
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Inflammatory Infiltrate microvessel density vascular endothelial growth factor nitric oxide synthase and proliferative activity in soft tissues below intraorally welded titanium bars
Journal of Periodontology, 2010Co-Authors: Stefano Fanali, Adriano Piattelli, Marcello Piccirilli, Vittoria Perrotti, Luciano Riccardi, Laura Ricci, Luciano ArteseAbstract:Background: The aim of the present study is a comparative evaluation of Inflammatory Infiltrate, microvessel density, vascular endothelial growth factor, nitric oxide synthase, and proliferative activity in soft tissues below intraorally welded titanium bars. Methods: Twenty-two patients participated in this study. All patients carried immediately loaded one-stage titanium implants splinted with intraorally welded titanium bars. Each patient underwent two gingival biopsies, a control biopsy harvested from an area of mucosa 5 mm away from the titanium bar and a test biopsy from the mucosa below the titanium bar, which were histologically and immunohistochemically processed. Results: No fractures or radiographically detectable alterations of the welded frameworks were present. In all the cases examined, the average of the modified plaque index was 1, no suppuration or bleeding on probing was present, and probing depth was £3 mm. However, the immunohistochemical analysis revealed some differences. The Inflammatory Infiltrate was mostly present in test sites and its extension was much larger than in control sites. Statistically significant differences were found in microvessel density and Ki-67 expression among control and test groups (P <0.0001). The high intensity of vascular endothelial growth factor, nitric oxide synthase 1, and nitric oxide synthase 3 expression were mainly detected in the test group, whereas the low intensities were mostly expressed in controls, with statistically significant differences (P <0.0001). Conclusions: In the present study, the immunohistochemical analysis shows that the tissues below the titanium bars underwent a higher rate of Inflammatory and reparative processes. However, further long-term studies, where clinical and immunohistochemical data are collected in parallel, should be conducted for a better understanding of the expression pattern of inflammation markers. J Periodontol 2010;81:748-757.
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Inflammatory Infiltrate microvessel density nitric oxide synthase expression vascular endothelial growth factor expression and proliferative activity in peri implant soft tissues around titanium and zirconium oxide healing caps
Journal of Periodontology, 2006Co-Authors: Marco Degidi, Luciano Artese, Vittoria Perrotti, Antonio Scarano, Peter Gehrke, Adriano PiattelliAbstract:Background: The aim of the present study in humans was to conduct a comparative immunohistochemical evaluation of vascular endothelial growth factor (VEGF) and nitric oxide synthase (NOS) expression, Inflammatory Infiltrate, proliferative activity expression, and microvessel density (MVD) in peri-implant soft tissues of titanium and zirconium oxide healing caps.Methods: Five patients, three men and two women (aged 30 to 66 years; mean: 49 years), participated in this study. All patients received dental implants that were 3.8 mm in diameter and 11 mm in length. All implants were left to heal in a non-submerged (single-stage) mode. Healing caps (3.8 mm in diameter and 3.0 mm in height) were inserted in all implants. Half of the implants were supplied with standard, prefabricated caps of commercially pure titanium, whereas the other half were provided with test zirconium oxide caps. After a 6-month healing period, a gingival biopsy was performed with a circular scalpel (5.5 mm in diameter) around the healing...
Vittoria Perrotti - One of the best experts on this subject based on the ideXlab platform.
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immunohistochemical analysis of Inflammatory Infiltrate in aggressive and chronic periodontitis a comparative study
Clinical Oral Investigations, 2011Co-Authors: Luciano Artese, Maciej Simon, Adriano Piattelli, Daniel Ferrari, Luciana Ap Gouveia Cardoso, Marcelo Faveri, Tatiana Onuma, Marcello Piccirilli, Vittoria PerrottiAbstract:This immunohistochemical study evaluated the Inflammatory Infiltrate with its cluster differentiation markers (CD 4, CD 8, CD 20, and CD 68) in aggressive and chronic periodontitis gingival tissues in order to identify the specific cell distribution. Twenty-seven human gingival biopsies were obtained and analyzed. Fourteen patients were suffering from chronic periodontitis and six from aggressive periodontitis; seven patients with healthy gingiva were included as the control group. The specimens were immunohistochemically stained for anti-CD 4 (T helper cells), anti-CD 8 (T cytotoxic/suppressor), anti CD-20 (B plasma cells) and anti CD-68 (macrophages). Chronic periodontitis samples were mainly dominated by CD 4 and CD 8+ cells. On the contrary, in aggressive periodontitis patients all four cell types (CD 4, CD 8, CD 20 and CD 68 + cells, respectively) were remarkably increased. CD 20+ cells were significantly (p < 0.05) more prevalent in aggressive versus chronic periodontitis. The control samples expressed lower CD 4, CD 8, CD 20 and CD 68+ cells confirming a none Inflammatory state. The present study demonstrates prevalence for CD 20+ cells in aggressive periodontitis lesions. However, further studies need to be performed to confirm and identify a clear pattern of Inflammatory cells and hereafter the mechanisms sustaining the disease.
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Inflammatory Infiltrate microvessel density vascular endothelial growth factor nitric oxide synthase and proliferative activity in soft tissues below intraorally welded titanium bars
Journal of Periodontology, 2010Co-Authors: Stefano Fanali, Adriano Piattelli, Marcello Piccirilli, Vittoria Perrotti, Luciano Riccardi, Laura Ricci, Luciano ArteseAbstract:Background: The aim of the present study is a comparative evaluation of Inflammatory Infiltrate, microvessel density, vascular endothelial growth factor, nitric oxide synthase, and proliferative activity in soft tissues below intraorally welded titanium bars. Methods: Twenty-two patients participated in this study. All patients carried immediately loaded one-stage titanium implants splinted with intraorally welded titanium bars. Each patient underwent two gingival biopsies, a control biopsy harvested from an area of mucosa 5 mm away from the titanium bar and a test biopsy from the mucosa below the titanium bar, which were histologically and immunohistochemically processed. Results: No fractures or radiographically detectable alterations of the welded frameworks were present. In all the cases examined, the average of the modified plaque index was 1, no suppuration or bleeding on probing was present, and probing depth was £3 mm. However, the immunohistochemical analysis revealed some differences. The Inflammatory Infiltrate was mostly present in test sites and its extension was much larger than in control sites. Statistically significant differences were found in microvessel density and Ki-67 expression among control and test groups (P <0.0001). The high intensity of vascular endothelial growth factor, nitric oxide synthase 1, and nitric oxide synthase 3 expression were mainly detected in the test group, whereas the low intensities were mostly expressed in controls, with statistically significant differences (P <0.0001). Conclusions: In the present study, the immunohistochemical analysis shows that the tissues below the titanium bars underwent a higher rate of Inflammatory and reparative processes. However, further long-term studies, where clinical and immunohistochemical data are collected in parallel, should be conducted for a better understanding of the expression pattern of inflammation markers. J Periodontol 2010;81:748-757.
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Inflammatory Infiltrate microvessel density nitric oxide synthase expression vascular endothelial growth factor expression and proliferative activity in peri implant soft tissues around titanium and zirconium oxide healing caps
Journal of Periodontology, 2006Co-Authors: Marco Degidi, Luciano Artese, Vittoria Perrotti, Antonio Scarano, Peter Gehrke, Adriano PiattelliAbstract:Background: The aim of the present study in humans was to conduct a comparative immunohistochemical evaluation of vascular endothelial growth factor (VEGF) and nitric oxide synthase (NOS) expression, Inflammatory Infiltrate, proliferative activity expression, and microvessel density (MVD) in peri-implant soft tissues of titanium and zirconium oxide healing caps.Methods: Five patients, three men and two women (aged 30 to 66 years; mean: 49 years), participated in this study. All patients received dental implants that were 3.8 mm in diameter and 11 mm in length. All implants were left to heal in a non-submerged (single-stage) mode. Healing caps (3.8 mm in diameter and 3.0 mm in height) were inserted in all implants. Half of the implants were supplied with standard, prefabricated caps of commercially pure titanium, whereas the other half were provided with test zirconium oxide caps. After a 6-month healing period, a gingival biopsy was performed with a circular scalpel (5.5 mm in diameter) around the healing...
H Brito - One of the best experts on this subject based on the ideXlab platform.
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is the immunohistochemical study of the Inflammatory Infiltrate helpful in distinguishing villitis of unknown etiology from non specific infection villitis
Placenta, 2005Co-Authors: H Brito, Priscila Bianchi Juliano, C Altemani, Albina AltemaniAbstract:Villitis is an important placental lesion, which can be caused by specific maternal infections, e.g. rubella, toxoplasmosis, etc, but most of them are of unknown etiology [1]. The clinical significance of villitis depends on the etiology and severity of fetal infection [2] and may lead to intrauterine fetal death, abortion, malformations and fetal growth retardation. The latter has been the major clinical association of villitis of unknown etiology (VUE) [2e4]. Morphologically, infectious villitis may show no specific features and can masquerade as VUE [5]. Both lesions are characterized by chronic Inflammatory cells, mainly macrophages and lymphocytes, in the placental villous stroma [2,5]. There are few immunomorphological studies about the immune cells in infectious villitis as well as in VUE [6e9]. Labarrere et al. [6] described that in placentas with VUE the predominant Inflammatory cells were activated macrophages expressing class II major histocompatibility complex antigens and helper T cells. Few CD8C T cells and no B cells were detected in the same lesions [6]. These findings contrast with those in villitis caused by maternal infection by Trypanosoma cruzi (Chagas’ disease), in which CD8C cells outnumbered CD4C T cells [7]. The aim of this study was to analyze whether immunohistochemical study of the Inflammatory Infiltrate in chronic villitis without specific morphological features could distinguish VUE from infectious villitis. All cases of villitis should be reported, particularly in developing countries, where there is a higher incidence of intrauterine infection [10] and the immunohistochemical study could provide objectivity that conventional histological methods lack.