The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Michael S Strano - One of the best experts on this subject based on the ideXlab platform.
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detection of dna hybridization using the near Infrared Band gap fluorescence of single walled carbon nanotubes
Nano Letters, 2006Co-Authors: Esther S Jeng, Anthonie E Moll, Joseph B Gastala, Michael S StranoAbstract:We demonstrate the optical detection of DNA hybridization on the surface of solution suspended single-walled carbon nanotubes (SWNTs) through a SWNT Band gap fluorescence modulation. Hybridization of a 24-mer oligonucleotide sequence with its complement produces a hypsochromic shift of 2 meV, with a detection sensitivity of 6 nM. The energy shift is modeled by correlating the surface coverage of DNA on SWNT to the exciton binding energy, yielding an estimated initial fractional coverage of 0.25 and a final coverage of 0.5. Hybridization on the nanotube surface is confirmed using Forster resonance energy transfer of fluorophore-labeled DNA oligonucleotides. This detection is enabled through a new technique to suspend SWNTs using adsorption of single-stranded DNA and subsequent removal of free DNA from solution. While the kinetics of free DNA hybridization are relatively fast (<10 min), the kinetics of the process on SWNTs are slower under comparable conditions, reaching steady state after 13 h at 25 °C. A ...
Esther S Jeng - One of the best experts on this subject based on the ideXlab platform.
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detection of dna hybridization using the near Infrared Band gap fluorescence of single walled carbon nanotubes
Nano Letters, 2006Co-Authors: Esther S Jeng, Anthonie E Moll, Joseph B Gastala, Michael S StranoAbstract:We demonstrate the optical detection of DNA hybridization on the surface of solution suspended single-walled carbon nanotubes (SWNTs) through a SWNT Band gap fluorescence modulation. Hybridization of a 24-mer oligonucleotide sequence with its complement produces a hypsochromic shift of 2 meV, with a detection sensitivity of 6 nM. The energy shift is modeled by correlating the surface coverage of DNA on SWNT to the exciton binding energy, yielding an estimated initial fractional coverage of 0.25 and a final coverage of 0.5. Hybridization on the nanotube surface is confirmed using Forster resonance energy transfer of fluorophore-labeled DNA oligonucleotides. This detection is enabled through a new technique to suspend SWNTs using adsorption of single-stranded DNA and subsequent removal of free DNA from solution. While the kinetics of free DNA hybridization are relatively fast (<10 min), the kinetics of the process on SWNTs are slower under comparable conditions, reaching steady state after 13 h at 25 °C. A ...
Mustafa Cebe - One of the best experts on this subject based on the ideXlab platform.
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Solvents effect on Infrared spectra of trimethyl phosphate in organic solvents
Vibrational Spectroscopy, 2004Co-Authors: Nalan Tekin, Mustafa CebeAbstract:Abstract Infrared spectroscopy studies of trimethyl phosphate (TMP) in 17 different pure organic solvents were undertaken to investigate the solvent–solute interactions and to correlate solvent properties such as the Kirkwood–Bauer–Magat (KBM) equation, the solvent acceptor number (AN) and the linear solvation energy relationships (LSER), respectively, with the Infrared Band shift. Poor correlation between dielectric constants and the frequencies showed that the KBM relationship was unsuitable for the investigated molecules. These frequencies exhibited a better correlation with the LSER than the solvent AN.
Austin J. Barnes - One of the best experts on this subject based on the ideXlab platform.
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Solvent effects on Infrared, 13C and 31P NMR spectra of trimethyl phosphate: Part 1. Single solvent systems
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 1999Co-Authors: Roman Streck, Austin J. BarnesAbstract:Abstract Infrared and multinuclear magnetic resonance spectroscopy (NMR) studies of trimethyl phosphate in 24 different solvents were undertaken to investigate the solute–solvent interactions and to correlate solvent properties such as acceptor number and dielectric constant with the Infrared Band shift and the 13C and 31P NMR chemical shifts. Furthermore the cross-correlation between the Infrared and NMR shifts in different solvents was studied. The results demonstrate that in general there is a correlation between IR and NMR shifts: the Infrared, 13C and 31P shifts can be predicted for most solvents used in this study, if one of them is known.
Joseph B Gastala - One of the best experts on this subject based on the ideXlab platform.
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detection of dna hybridization using the near Infrared Band gap fluorescence of single walled carbon nanotubes
Nano Letters, 2006Co-Authors: Esther S Jeng, Anthonie E Moll, Joseph B Gastala, Michael S StranoAbstract:We demonstrate the optical detection of DNA hybridization on the surface of solution suspended single-walled carbon nanotubes (SWNTs) through a SWNT Band gap fluorescence modulation. Hybridization of a 24-mer oligonucleotide sequence with its complement produces a hypsochromic shift of 2 meV, with a detection sensitivity of 6 nM. The energy shift is modeled by correlating the surface coverage of DNA on SWNT to the exciton binding energy, yielding an estimated initial fractional coverage of 0.25 and a final coverage of 0.5. Hybridization on the nanotube surface is confirmed using Forster resonance energy transfer of fluorophore-labeled DNA oligonucleotides. This detection is enabled through a new technique to suspend SWNTs using adsorption of single-stranded DNA and subsequent removal of free DNA from solution. While the kinetics of free DNA hybridization are relatively fast (<10 min), the kinetics of the process on SWNTs are slower under comparable conditions, reaching steady state after 13 h at 25 °C. A ...
