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D Fagan - One of the best experts on this subject based on the ideXlab platform.
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British Journal of Anaesthesia 1994; 73: 820-825 Inhalation Anaesthetic competition at high-affinity cocaine binding sites in rat brain synaptosomes
2016Co-Authors: Roderic G Eckenhoff, D FaganAbstract:We have shown previously that Inhalation Anaesthetics inhibit dopamine transport in rat synaptosomes. In order to determine if this in-hibition is associated with occupancy of the cocaine site, we examined binding of [3H](2p~-carbomethoxy-3p-(4-fluorophenyl)-tropane) (3H-CFT) in the presence of halothane or isoflurane 0.01-5 mmol litre"1 in rat brain synaptosomes. Both Anaesthetics inhibited 3H-CFT binding (mean /Ci 0.61 (SEMO.12) and 0.75 (0.21) mmol litre"1, respectively), by increasing /Cd (13.8 (0.6) and 29.8 (12.8) nmol litre"1, respectively) compared with control (8.02 (0.5) nmol litre"1) {P < 0.01). Halothane did not change fimax, but isoflurane increased it significantly. Cocaine protected CFT sites from /V-ethylmaleimide alkylation, but neither Anaesthetic did. Photoaffinity labelling with halothane significantly inhibited 3H-CFT binding compared with UV-exposed controls. We conclude that clinically relevant concentrations of both Anaesthetics inhibit high-affinity CFT binding, and the data suggest overlapping sites for halothan
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Inhalation Anaesthetic competition at high affinity cocaine binding sites in rat brain synaptosomes
BJA: British Journal of Anaesthesia, 1994Co-Authors: Roderic G Eckenhoff, D FaganAbstract:We have shown previously that Inhalation Anaesthetics inhibit dopamine transport in rat synaptosomes. In order to determine if this inhibition is associated with occupancy of the cocaine site, we examined binding of [3H] (2 beta-carbomethoxy-3 beta-(4-fluorophenyl)-tropane) (3H-CFT) in the presence of halothane or isoflurane 0.01-5 mmol litre-1 in rat brain synaptosomes. Both Anaesthetics inhibited 3H-CFT binding (mean Ki 0.61 (SEM 0.12) and 0.75 (0.21) mmol litre-1, respectively), by increasing Kd (13.8 (0.6) and 29.8 (12.8) nmol litre-1, respectively) compared with control (8.02 (0.5) nmol litre-1) (P < 0.01). Halothane did not change Bmax, but isoflurane increased it significantly. Cocaine protected CFT sites from N-ethylmaleimide alkylation, but neither Anaesthetic did. Photoaffinity labelling with halothane significantly inhibited 3H-CFT binding compared with UV-exposed controls. We conclude that clinically relevant concentrations of both Anaesthetics inhibit high-affinity CFT binding, and the data suggest overlapping sites for halothane and CFT.
Roderic G Eckenhoff - One of the best experts on this subject based on the ideXlab platform.
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British Journal of Anaesthesia 1994; 73: 820-825 Inhalation Anaesthetic competition at high-affinity cocaine binding sites in rat brain synaptosomes
2016Co-Authors: Roderic G Eckenhoff, D FaganAbstract:We have shown previously that Inhalation Anaesthetics inhibit dopamine transport in rat synaptosomes. In order to determine if this in-hibition is associated with occupancy of the cocaine site, we examined binding of [3H](2p~-carbomethoxy-3p-(4-fluorophenyl)-tropane) (3H-CFT) in the presence of halothane or isoflurane 0.01-5 mmol litre"1 in rat brain synaptosomes. Both Anaesthetics inhibited 3H-CFT binding (mean /Ci 0.61 (SEMO.12) and 0.75 (0.21) mmol litre"1, respectively), by increasing /Cd (13.8 (0.6) and 29.8 (12.8) nmol litre"1, respectively) compared with control (8.02 (0.5) nmol litre"1) {P < 0.01). Halothane did not change fimax, but isoflurane increased it significantly. Cocaine protected CFT sites from /V-ethylmaleimide alkylation, but neither Anaesthetic did. Photoaffinity labelling with halothane significantly inhibited 3H-CFT binding compared with UV-exposed controls. We conclude that clinically relevant concentrations of both Anaesthetics inhibit high-affinity CFT binding, and the data suggest overlapping sites for halothan
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Inhalation Anaesthetic competition at high affinity cocaine binding sites in rat brain synaptosomes
BJA: British Journal of Anaesthesia, 1994Co-Authors: Roderic G Eckenhoff, D FaganAbstract:We have shown previously that Inhalation Anaesthetics inhibit dopamine transport in rat synaptosomes. In order to determine if this inhibition is associated with occupancy of the cocaine site, we examined binding of [3H] (2 beta-carbomethoxy-3 beta-(4-fluorophenyl)-tropane) (3H-CFT) in the presence of halothane or isoflurane 0.01-5 mmol litre-1 in rat brain synaptosomes. Both Anaesthetics inhibited 3H-CFT binding (mean Ki 0.61 (SEM 0.12) and 0.75 (0.21) mmol litre-1, respectively), by increasing Kd (13.8 (0.6) and 29.8 (12.8) nmol litre-1, respectively) compared with control (8.02 (0.5) nmol litre-1) (P < 0.01). Halothane did not change Bmax, but isoflurane increased it significantly. Cocaine protected CFT sites from N-ethylmaleimide alkylation, but neither Anaesthetic did. Photoaffinity labelling with halothane significantly inhibited 3H-CFT binding compared with UV-exposed controls. We conclude that clinically relevant concentrations of both Anaesthetics inhibit high-affinity CFT binding, and the data suggest overlapping sites for halothane and CFT.
Paulo Do Nascimento - One of the best experts on this subject based on the ideXlab platform.
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intravenous versus Inhalation anaesthesia for one lung ventilation
Cochrane Database of Systematic Reviews, 2013Co-Authors: Norma Sueli Pinheiro Modolo, Marilia P Modolo, Marcos A Marton, Enilze De Souza Nogueira Volpato, Vinicius Monteiro Arantes, Paulo Do NascimentoAbstract:Background This is an update of a Cochrane Review first published in The Cochrane Library, Issue 2, 2008. The technique called one-lung ventilation can confine bleeding or infection to one lung, prevent rupture of a lung cyst or, more commonly, facilitate surgical exposure of the unventilated lung. During one-lung ventilation, anaesthesia is maintained either by delivering an Inhalation Anaesthetic to the ventilated lung or by infusing an intravenous Anaesthetic. It is possible that the method chosen to maintain anaesthesia may affect patient outcomes. Inhalation Anaesthetics may impair hypoxic pulmonary vasoconstriction (HPV) and increase intrapulmonary shunt and hypoxaemia. Objectives The objective of this review was to evaluate the effectiveness and safety of intravenous versus Inhalation anaesthesia for one-lung ventilation. Search methods We searched the Cochrane Central Register of Controlled Trials (CENTRAL); The Cochrane Library (2012, Issue 11); MEDLINE (1966 to November 2012); Embase (1980 to November 2012); Literatura Latino-Americana e do Caribe em Ciencias da Saude (LILACS, 1982 to November 2012) and ISI web of Science (1945 to November 2012), reference lists of identified trials and bibliographies of published reviews. We also contacted researchers in the field. No language restrictions were applied. The date of the most recent search was 19 November 2012. The original search was performed in June 2006. We reran the search in CENTRAL, MEDLINE, Embase, LILACS, and ISI web of Science in February 2017 and found four potential studies of interest which have been added to a list of 'Studies awaiting Classification' and will be incorporated into the formal review findings during the review update. Selection criteria We included randomized controlled trials and quasi-randomized controlled trials of intravenous (e.g. propofol) versus Inhalation (e.g. isoflurane, sevoflurane, desflurane) anaesthesia for one-lung ventilation in both surgical and intensive care participants. We excluded studies of participants who had only one lung (i.e. pneumonectomy or congenital absence of one lung). Data collection and analysis Two review authors independently assessed trial quality and extracted data. We contacted study authors for additional information. Main results We included in this updated review 20 studies that enrolled 850 participants, all of which assessed surgical participants－no studies investigated one-lung ventilation performed outside the operating theatre. No evidence indicated that the drug used to maintain anaesthesia during one-lung ventilation affected participant outcomes. The methodological quality of the included studies was difficult to assess as it was reported poorly, so the predominant classification of bias was 'unclear'. Authors' conclusions Very little evidence from randomized controlled trials suggests differences in participant outcomes with anaesthesia maintained by intravenous versus Inhalational anaesthesia during one-lung ventilation. If researchers believe that the type of drug used to maintain anaesthesia during one-lung ventilation is important, they should design randomized controlled trials with appropriate participant outcomes, rather than report temporary fluctuations in physiological variables.
Thomas Ledowski - One of the best experts on this subject based on the ideXlab platform.
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augmentation of the neuromuscular blocking effects of cisatracurium during desflurane sevoflurane isoflurane or total i v anaesthesia
BJA: British Journal of Anaesthesia, 1998Co-Authors: Hinnerk Wulf, M Kahl, Thomas LedowskiAbstract:We have evaluated the enhancement of cisatracurium-induced neuromuscular block by potent Inhalation Anaesthetic agents, by constructing dose-effect curves for cisatracurium in 84 patients during anaesthesia with 1.5 MAC (70% nitrous oxide) desflurane, sevoflurane, isoflurane or total i.v. anaesthesia (TIVA). Acceleromyography (TOF-Guard) and train-of-four (TOF) stimulation of the ulnar nerve were used (2 Hz every 12 s). Cisatracurium was administered in increments of 15 micrograms kg-1 until depression of T1/T0 > 95% was reached. ANOVA was used for statistical analysis (alpha = 0.05, beta = 0.2). Depression of T1/T0 during potent Inhalation anaesthesia was enhanced compared with TIVA. ED50 and ED95 values of cisatracurium were 15 (SD 5) and 34 (10) micrograms kg-1 for desflurane; 15 (4) and 32 (7) micrograms kg-1 for sevoflurane; and 15 (5) and 33 (9) micrograms kg-1 for isoflurane. These were significantly lower than the values for TIVA (21 (4) and 51 (13) micrograms kg-1) (P < 0.01 in each case). After equi-effective dosing, times to T1/T0 = 25% were similar in all groups (19 (7), 19 (5), 20 (5) vs 16 (4) min). Recovery index25-75% and time to a TOF ration of 0.70 were prolonged significantly by desflurane and sevoflurane compared with TIVA (18 (5), 19 (8) vs 12 (4) min and 43 (11), 44 (10) vs 35 (5) min, respectively), whereas the difference was not significant for isoflurane (14 (6) and 41 (7) min).
P. F. M. M. Correia - One of the best experts on this subject based on the ideXlab platform.
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Adsorption of the Inhalation Anaesthetic isoflurane by activated carbon fibres with reference data on non-porous carbon
Adsorption, 2020Co-Authors: M. Ribeiro Carrott, P. J. M. Carrott, P. F. M. M. CorreiaAbstract:Activated carbon fibres with a range of pore sizes, prepared from pre-oxidized acrylic fibre by physical activation in CO_2 at different temperatures and times, were investigated as adsorbents for the Inhalation Anaesthetic isoflurane (2-chloro-2-(difluoromethoxy)-1,1,1-trifluoroethane). Adsorption–desorption isotherms of isoflurane were determined at 293 K and were analysed by the α_S method using reference adsorption data, determined also at 293 K, on non-porous carbon. One of the samples obtained showed a much higher adsorption uptake at 100 mbar than reported in previous studies on different materials, while another sample not only showed a higher adsorption uptake than reported previously, but also a higher adsorption uptake at a pressure as low as 1 mbar showing potential for the capture of isoflurane. The results indicate that isoflurane does not adsorb in fine ultramicropores, of width less than about 0.5 nm. Comparison of the pore size distributions of these materials indicated that the presence of supermicropores, as well as ultramicropores, is relevant for the adsorption of isoflurane even at trace levels. Reference adsorption data for isoflurane on non-porous carbon is also presented.