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Roger Y Dodd - One of the best experts on this subject based on the ideXlab platform.
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limiting and detecting bacterial contamination of apheresis platelets Inlet Line diversion and increased culture volume improve component safety
Transfusion, 2009Co-Authors: Anne F Eder, Jean M Kennedy, Beth A Dy, Edward P Notari, Robert Skeate, Gary Bachowski, David C Mair, Jonathan S Webb, Stephen J Wagner, Roger Y DoddAbstract:BACKGROUND: Septic transfusion reactions to apheresis platelets (PLTs) continue to occur despite preventive measures. This study evaluated the effect of two operational changes designed to reduce bacterial risk: 1) introducing Inlet-Line sample diversion on two-arm procedures and 2) increasing the sample volume cultured from 4 to 8 mL from all donations. STUDY DESIGN AND METHODS: Aerobic culture results and septic transfusion reactions reported between December 1, 2006, and July 31, 2008 (Period 2), were compared to March 1, 2004, to May 31, 2006 (Period 1). RESULTS: During Period 2, a total of 781,936 apheresis PLT collections were cultured, of which 130 donations (1:6015) were confirmed positive and 9 (1:86,882) had negative culture results but were associated with 11 septic reactions. Confirmed-positive cultures from two-arm procedures decreased (27.2 to 14.7 per 105 collections; odds ratio [OR], 0.54; 95% confidence interval [CI], 0.41-0.70) in Period 2, owing to a lower rate of skin flora contamination. Detection of contamination of one-arm collections significantly increased by 54% in Period 2 (13.7 vs. 21.1 per 105 collections; OR, 1.54; 95% CI, 1.05-2.27). Fewer septic transfusion reactions occurred in Period 2, but the difference did not reach significance (1.7 vs. 1.2 per 105 donations; OR, 0.68; 95% CI, 0.30-1.53). CONCLUSION: Inlet-Line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.
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Limiting and detecting bacterial contamination of apheresis platelets: Inlet‐Line diversion and increased culture volume improve component safety
Transfusion, 2009Co-Authors: Anne F Eder, Jean M Kennedy, Beth A Dy, Edward P Notari, Robert Skeate, Gary Bachowski, David C Mair, Jonathan S Webb, Stephen J Wagner, Roger Y DoddAbstract:BACKGROUND: Septic transfusion reactions to apheresis platelets (PLTs) continue to occur despite preventive measures. This study evaluated the effect of two operational changes designed to reduce bacterial risk: 1) introducing Inlet-Line sample diversion on two-arm procedures and 2) increasing the sample volume cultured from 4 to 8 mL from all donations. STUDY DESIGN AND METHODS: Aerobic culture results and septic transfusion reactions reported between December 1, 2006, and July 31, 2008 (Period 2), were compared to March 1, 2004, to May 31, 2006 (Period 1). RESULTS: During Period 2, a total of 781,936 apheresis PLT collections were cultured, of which 130 donations (1:6015) were confirmed positive and 9 (1:86,882) had negative culture results but were associated with 11 septic reactions. Confirmed-positive cultures from two-arm procedures decreased (27.2 to 14.7 per 105 collections; odds ratio [OR], 0.54; 95% confidence interval [CI], 0.41-0.70) in Period 2, owing to a lower rate of skin flora contamination. Detection of contamination of one-arm collections significantly increased by 54% in Period 2 (13.7 vs. 21.1 per 105 collections; OR, 1.54; 95% CI, 1.05-2.27). Fewer septic transfusion reactions occurred in Period 2, but the difference did not reach significance (1.7 vs. 1.2 per 105 donations; OR, 0.68; 95% CI, 0.30-1.53). CONCLUSION: Inlet-Line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.
Anne F Eder - One of the best experts on this subject based on the ideXlab platform.
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Apheresis technology correlates with bacterial contamination of platelets and reported septic transfusion reactions
Transfusion, 2017Co-Authors: Anne F Eder, Beth A Dy, Stephen J Wagner, Barbara Demerse, Susan L. Stramer, E. Mary O'neill, Ross M. HerronAbstract:BACKGROUND Apheresis technology to collect platelet (PLT) components differs among devices. We evaluated the relationship of the plateletpheresis device with bacterial contamination and reported septic transfusion reactions. STUDY DESIGN AND METHODS Plateletpheresis was performed using Amicus (Fenwal, a Fresenius Kabi Company) or Trima (Trima Accel, TerumoBCT) from 2010 to 2014. All donations used Inlet-Line sample diversion and were tested by quality control (QC; Day 1) aerobic culture. Rates of bacterial contamination and septic reactions to PLTs were calculated for both devices. RESULTS During the 5-year study period, plateletpheresis collections using Amicus and Trima devices totaled 1,486,888 and 671,955 donations, respectively. The rate of confirmed-positive bacterial cultures of apheresis PLT donations was significantly higher with Amicus than with Trima (252 vs. 112 per 106 donations [odds ratio {OR}, 2.3; 95% confidence interval {CI}, 1.8-2.9]). Septic transfusion reactions were caused by 30 apheresis PLT units from 25 contaminated Amicus procedures and three apheresis PLT units from three contaminated Trima procedures. The overall rate of septic reactions was significantly higher with apheresis PLT components collected with Amicus than with Trima (16.8 vs. 4.5 per 106 donations [OR, 3.8; 95% CI, 1.1-12.5]). All apheresis PLT components implicated in septic transfusion reactions had negative QC culture results incubated through Day 5 (i.e., false negatives). CONCLUSION Apheresis technology affects bacterial contamination of plateletpheresis collections. The device-specific, higher rate of confirmed-positive bacterial culture results also correlated with a significantly higher rate of reported septic transfusion reactions to apheresis PLTs.
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limiting and detecting bacterial contamination of apheresis platelets Inlet Line diversion and increased culture volume improve component safety
Transfusion, 2009Co-Authors: Anne F Eder, Jean M Kennedy, Beth A Dy, Edward P Notari, Robert Skeate, Gary Bachowski, David C Mair, Jonathan S Webb, Stephen J Wagner, Roger Y DoddAbstract:BACKGROUND: Septic transfusion reactions to apheresis platelets (PLTs) continue to occur despite preventive measures. This study evaluated the effect of two operational changes designed to reduce bacterial risk: 1) introducing Inlet-Line sample diversion on two-arm procedures and 2) increasing the sample volume cultured from 4 to 8 mL from all donations. STUDY DESIGN AND METHODS: Aerobic culture results and septic transfusion reactions reported between December 1, 2006, and July 31, 2008 (Period 2), were compared to March 1, 2004, to May 31, 2006 (Period 1). RESULTS: During Period 2, a total of 781,936 apheresis PLT collections were cultured, of which 130 donations (1:6015) were confirmed positive and 9 (1:86,882) had negative culture results but were associated with 11 septic reactions. Confirmed-positive cultures from two-arm procedures decreased (27.2 to 14.7 per 105 collections; odds ratio [OR], 0.54; 95% confidence interval [CI], 0.41-0.70) in Period 2, owing to a lower rate of skin flora contamination. Detection of contamination of one-arm collections significantly increased by 54% in Period 2 (13.7 vs. 21.1 per 105 collections; OR, 1.54; 95% CI, 1.05-2.27). Fewer septic transfusion reactions occurred in Period 2, but the difference did not reach significance (1.7 vs. 1.2 per 105 donations; OR, 0.68; 95% CI, 0.30-1.53). CONCLUSION: Inlet-Line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.
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Limiting and detecting bacterial contamination of apheresis platelets: Inlet‐Line diversion and increased culture volume improve component safety
Transfusion, 2009Co-Authors: Anne F Eder, Jean M Kennedy, Beth A Dy, Edward P Notari, Robert Skeate, Gary Bachowski, David C Mair, Jonathan S Webb, Stephen J Wagner, Roger Y DoddAbstract:BACKGROUND: Septic transfusion reactions to apheresis platelets (PLTs) continue to occur despite preventive measures. This study evaluated the effect of two operational changes designed to reduce bacterial risk: 1) introducing Inlet-Line sample diversion on two-arm procedures and 2) increasing the sample volume cultured from 4 to 8 mL from all donations. STUDY DESIGN AND METHODS: Aerobic culture results and septic transfusion reactions reported between December 1, 2006, and July 31, 2008 (Period 2), were compared to March 1, 2004, to May 31, 2006 (Period 1). RESULTS: During Period 2, a total of 781,936 apheresis PLT collections were cultured, of which 130 donations (1:6015) were confirmed positive and 9 (1:86,882) had negative culture results but were associated with 11 septic reactions. Confirmed-positive cultures from two-arm procedures decreased (27.2 to 14.7 per 105 collections; odds ratio [OR], 0.54; 95% confidence interval [CI], 0.41-0.70) in Period 2, owing to a lower rate of skin flora contamination. Detection of contamination of one-arm collections significantly increased by 54% in Period 2 (13.7 vs. 21.1 per 105 collections; OR, 1.54; 95% CI, 1.05-2.27). Fewer septic transfusion reactions occurred in Period 2, but the difference did not reach significance (1.7 vs. 1.2 per 105 donations; OR, 0.68; 95% CI, 0.30-1.53). CONCLUSION: Inlet-Line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.
S.e. Aleman - One of the best experts on this subject based on the ideXlab platform.
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DEGB LOCA ECS power limit recommendation for the K-14. 1 subcycle
1991Co-Authors: F.g. Smith, S.e. AlemanAbstract:This report documents assembly deposited power limits and the corresponding effluent temperature limits recommended for operating the K-14.1 subcycle to ensure sufficient cooling of reactor assemblies during the ECS phase of a Double Ended Guillotine Break (DEGSS) Loss of Coolant Accident (LOCA). The ECS LOCA effluent temperature limits are computed for each flowzone of the K-14.1 charge. The recommended overall DEGB LOCA ECS power limit is 1515 MW or about 63.1% of the historical full reactor power limit (assumed to be 2400-MW) for Mark 22 assemblies. The design basis accident is a break in the plenum Inlet Line where the AC pump motors not tripped.
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DEGB LOCA ECS power limit recommendation for the K-14.1 subcycle. Revision 1
1991Co-Authors: F.g. Smith, S.e. AlemanAbstract:This report documents assembly deposited power limits and the corresponding effluent temperature limits recommended for operating the K-14.1 subcycle to ensure sufficient cooling of reactor assemblies during the ECS phase of a Double Ended Guillotine Break (DEGSS) Loss of Coolant Accident (LOCA). The ECS LOCA effluent temperature limits are computed for each flowzone of the K-14.1 charge. The recommended overall DEGB LOCA ECS power limit is 1515 MW or about 63.1% of the historical full reactor power limit (assumed to be 2400-MW) for Mark 22 assemblies. The design basis accident is a break in the plenum Inlet Line where the AC pump motors not tripped.
M. Kulmala - One of the best experts on this subject based on the ideXlab platform.
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Operation of the Airmodus A11 nano Condensation Nucleus Counter at various Inlet pressures, various operation temperatures and design of a new Inlet system
Atmospheric Measurement Techniques Discussions, 2015Co-Authors: J. Kangasluoma, A. Franchin, J. Duplissy, L. Ahonen, F. Korhonen, M. Attoui, J. Mikkilä, K. Lehtipalo, J. Vanhanen, M. KulmalaAbstract:Abstract. Measuring sub-3 nm particles outside of controlled laboratory conditions is a challenging task, as many of the instruments are operated at their limits and are subjected to changing ambient conditions. In this study, we advance the current understanding on the operation of Airmodus A11 nano Condensation Nucleus Counter (nCNC), which consists of a A10 Particle Size Magnifier (PSM) and A20 condensation particle counter (CPC). We explore the effect of the Inlet Line pressure on the measured particle concentration. We identify two different regions inside the instrument where supersaturation of working fluid can take place. We show the possibility of varying the cut-off of the instrument from 1 to 6 nm, a wider size range than the one usually covered by the PSM. We also present a new Inlet system, which allows automated measurements of the background, minimizes the diffusion losses in the sampling Line and is equipped with an electrostatic filter to remove ions. Finally, our view of the guideLines for optimal use of the Airmodus nCNC are provided.
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Operation of the Airmodus A11 nano Condensation Nucleus Counter at variousInlet pressures and various operation temperatures, and design of a new Inletsystem
Atmospheric Measurement Techniques, 2015Co-Authors: J. Kangasluoma, A. Franchin, J. Duplissy, L. Ahonen, F. Korhonen, M. Attoui, J. Mikkilä, K. Lehtipalo, J. Vanhanen, M. KulmalaAbstract:Abstract. Measuring sub-3 nm particles outside of controlled laboratory conditions is a challenging task, as many of the instruments are operated at their limits and are subject to changing ambient conditions. In this study, we advance the current understanding of the operation of the Airmodus A11 nano Condensation Nucleus Counter (nCNC), which consists of an A10 Particle Size Magnifier (PSM) and an A20 Condensation Particle Counter (CPC). The effect of the Inlet Line pressure on the measured particle concentration was measured, and two separate regions inside the A10, where supersaturation of working fluid can take place, were identified. The possibility of varying the lower cut-off diameter of the nCNC was investigated; by scanning the growth tube temperature, the range of the lower cut-off was extended from 1–2.5 to 1–6 nm. Here we present a new Inlet system, which allows automated measurement of the background concentration of homogeneously nucleated droplets, minimizes the diffusion losses in the sampling Line and is equipped with an electrostatic filter to remove ions smaller than approximately 4.5 nm. Finally, our view of the guideLines for the optimal use of the Airmodus nCNC is provided.
Beth A Dy - One of the best experts on this subject based on the ideXlab platform.
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Apheresis technology correlates with bacterial contamination of platelets and reported septic transfusion reactions
Transfusion, 2017Co-Authors: Anne F Eder, Beth A Dy, Stephen J Wagner, Barbara Demerse, Susan L. Stramer, E. Mary O'neill, Ross M. HerronAbstract:BACKGROUND Apheresis technology to collect platelet (PLT) components differs among devices. We evaluated the relationship of the plateletpheresis device with bacterial contamination and reported septic transfusion reactions. STUDY DESIGN AND METHODS Plateletpheresis was performed using Amicus (Fenwal, a Fresenius Kabi Company) or Trima (Trima Accel, TerumoBCT) from 2010 to 2014. All donations used Inlet-Line sample diversion and were tested by quality control (QC; Day 1) aerobic culture. Rates of bacterial contamination and septic reactions to PLTs were calculated for both devices. RESULTS During the 5-year study period, plateletpheresis collections using Amicus and Trima devices totaled 1,486,888 and 671,955 donations, respectively. The rate of confirmed-positive bacterial cultures of apheresis PLT donations was significantly higher with Amicus than with Trima (252 vs. 112 per 106 donations [odds ratio {OR}, 2.3; 95% confidence interval {CI}, 1.8-2.9]). Septic transfusion reactions were caused by 30 apheresis PLT units from 25 contaminated Amicus procedures and three apheresis PLT units from three contaminated Trima procedures. The overall rate of septic reactions was significantly higher with apheresis PLT components collected with Amicus than with Trima (16.8 vs. 4.5 per 106 donations [OR, 3.8; 95% CI, 1.1-12.5]). All apheresis PLT components implicated in septic transfusion reactions had negative QC culture results incubated through Day 5 (i.e., false negatives). CONCLUSION Apheresis technology affects bacterial contamination of plateletpheresis collections. The device-specific, higher rate of confirmed-positive bacterial culture results also correlated with a significantly higher rate of reported septic transfusion reactions to apheresis PLTs.
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limiting and detecting bacterial contamination of apheresis platelets Inlet Line diversion and increased culture volume improve component safety
Transfusion, 2009Co-Authors: Anne F Eder, Jean M Kennedy, Beth A Dy, Edward P Notari, Robert Skeate, Gary Bachowski, David C Mair, Jonathan S Webb, Stephen J Wagner, Roger Y DoddAbstract:BACKGROUND: Septic transfusion reactions to apheresis platelets (PLTs) continue to occur despite preventive measures. This study evaluated the effect of two operational changes designed to reduce bacterial risk: 1) introducing Inlet-Line sample diversion on two-arm procedures and 2) increasing the sample volume cultured from 4 to 8 mL from all donations. STUDY DESIGN AND METHODS: Aerobic culture results and septic transfusion reactions reported between December 1, 2006, and July 31, 2008 (Period 2), were compared to March 1, 2004, to May 31, 2006 (Period 1). RESULTS: During Period 2, a total of 781,936 apheresis PLT collections were cultured, of which 130 donations (1:6015) were confirmed positive and 9 (1:86,882) had negative culture results but were associated with 11 septic reactions. Confirmed-positive cultures from two-arm procedures decreased (27.2 to 14.7 per 105 collections; odds ratio [OR], 0.54; 95% confidence interval [CI], 0.41-0.70) in Period 2, owing to a lower rate of skin flora contamination. Detection of contamination of one-arm collections significantly increased by 54% in Period 2 (13.7 vs. 21.1 per 105 collections; OR, 1.54; 95% CI, 1.05-2.27). Fewer septic transfusion reactions occurred in Period 2, but the difference did not reach significance (1.7 vs. 1.2 per 105 donations; OR, 0.68; 95% CI, 0.30-1.53). CONCLUSION: Inlet-Line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.
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Limiting and detecting bacterial contamination of apheresis platelets: Inlet‐Line diversion and increased culture volume improve component safety
Transfusion, 2009Co-Authors: Anne F Eder, Jean M Kennedy, Beth A Dy, Edward P Notari, Robert Skeate, Gary Bachowski, David C Mair, Jonathan S Webb, Stephen J Wagner, Roger Y DoddAbstract:BACKGROUND: Septic transfusion reactions to apheresis platelets (PLTs) continue to occur despite preventive measures. This study evaluated the effect of two operational changes designed to reduce bacterial risk: 1) introducing Inlet-Line sample diversion on two-arm procedures and 2) increasing the sample volume cultured from 4 to 8 mL from all donations. STUDY DESIGN AND METHODS: Aerobic culture results and septic transfusion reactions reported between December 1, 2006, and July 31, 2008 (Period 2), were compared to March 1, 2004, to May 31, 2006 (Period 1). RESULTS: During Period 2, a total of 781,936 apheresis PLT collections were cultured, of which 130 donations (1:6015) were confirmed positive and 9 (1:86,882) had negative culture results but were associated with 11 septic reactions. Confirmed-positive cultures from two-arm procedures decreased (27.2 to 14.7 per 105 collections; odds ratio [OR], 0.54; 95% confidence interval [CI], 0.41-0.70) in Period 2, owing to a lower rate of skin flora contamination. Detection of contamination of one-arm collections significantly increased by 54% in Period 2 (13.7 vs. 21.1 per 105 collections; OR, 1.54; 95% CI, 1.05-2.27). Fewer septic transfusion reactions occurred in Period 2, but the difference did not reach significance (1.7 vs. 1.2 per 105 donations; OR, 0.68; 95% CI, 0.30-1.53). CONCLUSION: Inlet-Line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.