The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Gilles Thuret - One of the best experts on this subject based on the ideXlab platform.
-
micro instillation of fluorescein with an Inoculation Loop for ocular surface staining in dry eye syndrome
Acta Ophthalmologica, 2017Co-Authors: Emilie Courrier, Didier Renault, Mathilde Kaspi, Agathe Marcon, Victor Lambert, Thibaud Garcin, F Chiambaretta, Gerhard Garhofer, Gilles ThuretAbstract:Purpose To describe and validate the micro-instillation of fluorescein on the ocular surface by a disposable calibrated Inoculation Loop to improve corneal and conjunctival staining quality. Methods Accuracy and precision of the volume of 0.5% sodium fluorescein collected by a single use 1 μl-calibrated Inoculation Loop were measured using a precision balance. Twenty patients (40 eyes) suffering from dry eye syndrome were enrolled in a prospective interventional nonrandomized study. Fluorescein was instilled with the Loop, and slit-lamp images were taken within 30 seconds using cobalt blue light with and without a yellow barrier filter. For comparison, after a washout period, the same images were retaken after instillation of one drop of fluorescein from a single-dose unit. The main outcome measure was the staining quality assessed by three experts, blind to the instillation method. Patient discomfort (tolerance, by a questionnaire) was also compared. Results The mean volume collected by the Loop was 1.18 ± 0.12 μl, compared with 33.70 ± 6.10 μl using the single-dose unit. The Loop avoided excess dye responsible for unpleasant tearing, masking of lesions and rapid diffusion into the stroma. Micro-instillation greatly improved image quality without losing information. The yellow filter further improved image contrast. Tolerance was excellent. Conclusion The 1 μl-calibrated Inoculation Loop is a safe, convenient, inexpensive, disposable, sterile, well-tolerated tool for reproducible micro-instillation of commercial fluorescein. By greatly improving staining quality, it will help standardize assessment of dry eye severity.
Emilie Courrier - One of the best experts on this subject based on the ideXlab platform.
-
micro instillation of fluorescein with an Inoculation Loop for ocular surface staining in dry eye syndrome
Acta Ophthalmologica, 2017Co-Authors: Emilie Courrier, Didier Renault, Mathilde Kaspi, Agathe Marcon, Victor Lambert, Thibaud Garcin, F Chiambaretta, Gerhard Garhofer, Gilles ThuretAbstract:Purpose To describe and validate the micro-instillation of fluorescein on the ocular surface by a disposable calibrated Inoculation Loop to improve corneal and conjunctival staining quality. Methods Accuracy and precision of the volume of 0.5% sodium fluorescein collected by a single use 1 μl-calibrated Inoculation Loop were measured using a precision balance. Twenty patients (40 eyes) suffering from dry eye syndrome were enrolled in a prospective interventional nonrandomized study. Fluorescein was instilled with the Loop, and slit-lamp images were taken within 30 seconds using cobalt blue light with and without a yellow barrier filter. For comparison, after a washout period, the same images were retaken after instillation of one drop of fluorescein from a single-dose unit. The main outcome measure was the staining quality assessed by three experts, blind to the instillation method. Patient discomfort (tolerance, by a questionnaire) was also compared. Results The mean volume collected by the Loop was 1.18 ± 0.12 μl, compared with 33.70 ± 6.10 μl using the single-dose unit. The Loop avoided excess dye responsible for unpleasant tearing, masking of lesions and rapid diffusion into the stroma. Micro-instillation greatly improved image quality without losing information. The yellow filter further improved image contrast. Tolerance was excellent. Conclusion The 1 μl-calibrated Inoculation Loop is a safe, convenient, inexpensive, disposable, sterile, well-tolerated tool for reproducible micro-instillation of commercial fluorescein. By greatly improving staining quality, it will help standardize assessment of dry eye severity.
C G De Los Reyesgavilan - One of the best experts on this subject based on the ideXlab platform.
-
invited review methods for the screening isolation and characterization of exopolysaccharides produced by lactic acid bacteria
Journal of Dairy Science, 2005Co-Authors: Patricia Ruasmadiedo, C G De Los ReyesgavilanAbstract:The ability to produce exopolysaccharides (EPS) is widespread among lactic acid bacteria (LAB), although the physiological role of these molecules has not been clearly established yet. Some EPS confer on LAB a "ropy" character that can be detected in cultures that form long strands when extended with an Inoculation Loop. When EPS are produced in situ during milk fermentation they can act as natural biothickeners, giving the product a suitable consistency, improving viscosity, and reducing syneresis. In addition, some of these EPS may have beneficial effects on human health. The increasing demand by consumers of novel dairy products requires a better understanding of the effect of EPS on existing products and, at the same time, the search for new EPS-producing strains with desirable properties. The use of genetically modified organisms capable of producing high levels of EPS or newly designed biopolymers is still very limited. Therefore, exploration of the biodiversity of wild LAB strains from natural ecological environments is currently the most suitable approach to search for the desired EPS-phenotype. The screening of ropy strains and the isolation and characterization of EPS responsible for this characteristic have led to the application over the past years of a wide variety of techniques. This review summarizes the available information on methods and procedures used for research on this topic. The information provided deals with methods for screening of EPS-producing LAB, detection of the ropy phenotype, and the physicochemical and structural characterization of these molecules, including parameters related to their viscosifying properties. To our knowledge, this is the first compilation of methods available for the study of EPS produced by LAB.
Didier Renault - One of the best experts on this subject based on the ideXlab platform.
-
micro instillation of fluorescein with an Inoculation Loop for ocular surface staining in dry eye syndrome
Acta Ophthalmologica, 2017Co-Authors: Emilie Courrier, Didier Renault, Mathilde Kaspi, Agathe Marcon, Victor Lambert, Thibaud Garcin, F Chiambaretta, Gerhard Garhofer, Gilles ThuretAbstract:Purpose To describe and validate the micro-instillation of fluorescein on the ocular surface by a disposable calibrated Inoculation Loop to improve corneal and conjunctival staining quality. Methods Accuracy and precision of the volume of 0.5% sodium fluorescein collected by a single use 1 μl-calibrated Inoculation Loop were measured using a precision balance. Twenty patients (40 eyes) suffering from dry eye syndrome were enrolled in a prospective interventional nonrandomized study. Fluorescein was instilled with the Loop, and slit-lamp images were taken within 30 seconds using cobalt blue light with and without a yellow barrier filter. For comparison, after a washout period, the same images were retaken after instillation of one drop of fluorescein from a single-dose unit. The main outcome measure was the staining quality assessed by three experts, blind to the instillation method. Patient discomfort (tolerance, by a questionnaire) was also compared. Results The mean volume collected by the Loop was 1.18 ± 0.12 μl, compared with 33.70 ± 6.10 μl using the single-dose unit. The Loop avoided excess dye responsible for unpleasant tearing, masking of lesions and rapid diffusion into the stroma. Micro-instillation greatly improved image quality without losing information. The yellow filter further improved image contrast. Tolerance was excellent. Conclusion The 1 μl-calibrated Inoculation Loop is a safe, convenient, inexpensive, disposable, sterile, well-tolerated tool for reproducible micro-instillation of commercial fluorescein. By greatly improving staining quality, it will help standardize assessment of dry eye severity.
Mathilde Kaspi - One of the best experts on this subject based on the ideXlab platform.
-
micro instillation of fluorescein with an Inoculation Loop for ocular surface staining in dry eye syndrome
Acta Ophthalmologica, 2017Co-Authors: Emilie Courrier, Didier Renault, Mathilde Kaspi, Agathe Marcon, Victor Lambert, Thibaud Garcin, F Chiambaretta, Gerhard Garhofer, Gilles ThuretAbstract:Purpose To describe and validate the micro-instillation of fluorescein on the ocular surface by a disposable calibrated Inoculation Loop to improve corneal and conjunctival staining quality. Methods Accuracy and precision of the volume of 0.5% sodium fluorescein collected by a single use 1 μl-calibrated Inoculation Loop were measured using a precision balance. Twenty patients (40 eyes) suffering from dry eye syndrome were enrolled in a prospective interventional nonrandomized study. Fluorescein was instilled with the Loop, and slit-lamp images were taken within 30 seconds using cobalt blue light with and without a yellow barrier filter. For comparison, after a washout period, the same images were retaken after instillation of one drop of fluorescein from a single-dose unit. The main outcome measure was the staining quality assessed by three experts, blind to the instillation method. Patient discomfort (tolerance, by a questionnaire) was also compared. Results The mean volume collected by the Loop was 1.18 ± 0.12 μl, compared with 33.70 ± 6.10 μl using the single-dose unit. The Loop avoided excess dye responsible for unpleasant tearing, masking of lesions and rapid diffusion into the stroma. Micro-instillation greatly improved image quality without losing information. The yellow filter further improved image contrast. Tolerance was excellent. Conclusion The 1 μl-calibrated Inoculation Loop is a safe, convenient, inexpensive, disposable, sterile, well-tolerated tool for reproducible micro-instillation of commercial fluorescein. By greatly improving staining quality, it will help standardize assessment of dry eye severity.